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Le Touquet – Paris-Plage, France

Grosso F.,University of Porto | Quinteira S.,University of Porto | Poirel L.,Service de Bacteriologie Virologie | Novais A.,University of Porto | Peixe L.,University of Porto
Antimicrobial Agents and Chemotherapy | Year: 2012

The spread of OXA-24/OXA-40 (OXA-24/40)-producing Acinetobacter spp. in the Iberian Peninsula has been strongly influenced by clonal expansion, but the role of horizontal gene transfer has scarcely been explored. bla OXA-24/OXA-40-carrying plasmids and genetic environments were characterized in representative (n = 15) Acinetobacter species clinical isolates (obtained between 2001 and 2007) by Acinetobacter baumannii PCR-based replicon typing, sequencing, hybridization, and restriction fragment length polymorphism. Besides the identification of bla OXA-24/40 within the chromosomes of some isolates, the circulation of common bla OXA-24/40-carrying plasmids (30-kb repA-AB; 10-kb aci2) and genetic backbones among Acinetobacter spp. was demonstrated. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source


Poirel L.,Service de Bacteriologie Virologie | Castanheira M.,JMI Laboratories | Carrer A.,Service de Bacteriologie Virologie | Rodriguez C.P.,Service de Bacteriologie Virologie | And 2 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2011

Two bla OXA-48-like-positive isolates (Klebsiella pneumoniae and Enterobacter cloacae) were recovered in Argentina in 2008 as part of a large-scale survey focused on multidrug resistance in Enterobacteriaceae. In both cases, sequencing identified β-lactamase OXA-163, differing from OXA-48 by a single amino substitution and a 4-amino-acid deletion. OXA-163 hydrolyzed penicillins, ceftazidime, and cefotaxime, whereas OXA-48 did not. However, OXA-163 had a much lower ability to hydrolyze carbapenems than OXA-48, therefore barely being considered a carbapenemase. In both isolates, the bla OXA-163 gene was located on plasmids that differed in structure and size. However, a detailed genetic analysis revealed a similar genetic context in those isolates, with the bla OXA-163 gene being bracketed by novel transposase genes, making this genetic environment different from that reported for the bla OXA-48 gene. This study identified the first class D β-lactamase compromising both extended-spectrum cephalosporin and carbapenem activities. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source


Dortet L.,Service de Bacteriologie Virologie | Poirel L.,Service de Bacteriologie Virologie | Nordmann P.,Service de Bacteriologie Virologie
Journal of Clinical Microbiology | Year: 2012

A novel biochemical technique, the Carba NP test, has been evaluated to detect carbapenemase production in Pseudomonas spp. This test was specific (100%), sensitive (94.4%), and rapid (<2 h). This cost-effective test, which could be implemented in any microbiology laboratory, offers a reliable technique for identification of carbapenemase-producing Pseudomonas spp. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source


Poirel L.,Service de Bacteriologie Virologie | Bonnin R.A.,Service de Bacteriologie Virologie | Nordmann P.,Service de Bacteriologie Virologie
Antimicrobial Agents and Chemotherapy | Year: 2011

The resistome of the multidrug-resistant Escherichia coli strain 271 carrying the plasmid-mediated blaNDM-1carbapenemase gene was analyzed by high-throughput genome sequencing. The p271A plasmid carrying the blaNDM-1 gene was 35.9 kb in size and possessed an IncN-type backbone that harbored a novel replicase gene. Acquisition of the blaNDM-1 gene on plasmid p271A had been likely the result of a cointegration event involving the transposase of Tn5403. The expression of blaNDM-1 was associated with the insertion sequence ISAba125 likely originating from Acinetobacter baumannii. E. coli 271 accumulated multiple resistance determinants, including five β-lactamase genes (comprising the extended-spectrum β-lactamase CTX-M-15), two 16S RNA methylase ArmA- and RmtB-encoding genes, and the qepA gene encoding an efflux pump involved in resistance to fluoroquinolones. These resistance genes were located on three additional plasmids, of 160 kb (IncA/C), 130 kb (IncF), and 110 kb (IncI1). In addition, several chromosomally encoded resistance determinants were identified, such as topoisomerase mutations, porin modifications and truncations, and the intrinsic ampC gene of E. coli that was weakly expressed. The multidrug resistance pattern observed for E. coli 271 was therefore the result of combined chromosome- and plasmid-encoded mechanisms. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source


Cuzon G.,Service de Bacteriologie Virologie | Naas T.,Service de Bacteriologie Virologie | Villegas M.-V.,International Center for Medical Research and Training | Correa A.,International Center for Medical Research and Training | And 2 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2011

Ten bla KPC-2-harboring Pseudomonas aeruginosa isolates from hospitals located in five different Colombian cities have been characterized. Isolates were multidrug resistant, belonged to five different pulsotypes, and possessed naturally chromosome-encoded bla AmpC and bla OXA-50 genes and the acquired bla KPC-2 gene. In most cases, the bla KPC-2 genes were carried by plasmids of different sizes and were associated with Tn4401b or a new structure containing only part of the Tn4401 sequence. This study revealed that several clones of P. aeruginosa producing bla KPC-2 are disseminating in Colombia. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source

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