Service dAnatomie Pathologique et Histologie Cytologie

Larrey, France

Service dAnatomie Pathologique et Histologie Cytologie

Larrey, France
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Vaillant O.,Max Mousseron Institute of Biomolecules | Cheikh K.E.,Max Mousseron Institute of Biomolecules | Warther D.,Charles Gerhardt Institute | Brevet D.,Charles Gerhardt Institute | And 15 more authors.
Angewandte Chemie - International Edition | Year: 2015

The development of personalized and non-invasive cancer therapies based on new targets combined with nanodevices is a major challenge in nanomedicine. In this work, the over-expression of a membrane lectin, the cation-independent mannose 6-phosphate receptor (M6PR), was specifically demonstrated in prostate cancer cell lines and tissues. To efficiently target this lectin a mannose-6-phosphate analogue was synthesized in six steps and grafted onto the surface of functionalized mesoporous silica nanoparticles (MSNs). These MSNs were used for in vitro and ex vivo photodynamic therapy to treat prostate cancer cell lines and primary cell cultures prepared from patient biopsies. The results demonstrated the efficiency of M6PR targeting for prostate cancer theranostic. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Vaillant O.,Max Mousseron Institute of Biomolecules | Cheikh K.E.,Max Mousseron Institute of Biomolecules | Warther D.,Charles Gerhardt Institute | Brevet D.,Charles Gerhardt Institute | And 16 more authors.
Angewandte Chemie - International Edition | Year: 2015

The development of personalized and non-invasive cancer therapies based on new targets combined with nanodevices is a major challenge in nanomedicine. In this work, the over-expression of a membrane lectin, the cation-independent mannose 6-phosphate receptor (M6PR), was specifically demonstrated in prostate cancer cell lines and tissues. To efficiently target this lectin a mannose-6-phosphate analogue was synthesized in six steps and grafted onto the surface of functionalized mesoporous silica nanoparticles (MSNs). These MSNs were used for in vitro and ex vivo photodynamic therapy to treat prostate cancer cell lines and primary cell cultures prepared from patient biopsies. The results demonstrated the efficiency of M6PR targeting for prostate cancer theranostic. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Gomez-Brouchet A.,Service dAnatomie Pathologique et Histologie Cytologie | Blaes N.,University Paul Sabatier | Mouledous L.,CNRS Institute of Pharmacology and Structural Biology | Fourcade O.,Toulouse University Hospital Center | And 4 more authors.
Journal of Translational Medicine | Year: 2015

Background: Diabetic neuropathy is one of the most common complications of diabetes and causes various problems in daily life. The aim of this study was to assess the effect of regional anaesthesia on post surgery opioid induced hyperalgesia in diabetic and non-diabetic mice. Methods: Diabetic and non-diabetic mice underwent plantar surgery. Levobupivacaine and sufentanil were used before surgery, for sciatic nerve block (regional anaesthesia) and analgesia, respectively. Diabetic and non-diabetic groups were each randomly assigned to three subgroups: control, no sufentanil and no levobupivacaine; sufentanil and no levobupivacaine; sufentanil and levobupivacaine. Three tests were used to assess pain behaviour: mechanical nociception; thermal nociception and guarding behaviours using a pain scale. Results: Sufentanil, alone or in combination with levobupivacaine, produced antinociceptive effects shortly after administration. Subsequently, sufentanil induced hyperalgesia in diabetic and non-diabetic mice. Opioid-induced hyperalgesia was enhanced in diabetic mice. Levobupivacaine associated to sufentanil completely prevented hyperalgesia in both groups of mice. Conclusion: The results suggest that regional anaesthesia can decrease opioid-induced hyperalgesia in diabetic as well as in non-diabetic mice. These observations may be clinically relevant for the management of diabetic patients. © 2015 Gomez-Brouchet et al.


Gomez-Brouchet A.,Service dAnatomie Pathologique et Histologie Cytologie | Blaes N.,University Paul Sabatier | Mouledous L.,CNRS Institute of Pharmacology and Structural Biology | Fourcade O.,Toulouse University Hospital Center | And 5 more authors.
Journal of Translational Medicine | Year: 2015

Background: Diabetic neuropathy is one of the most common complications of diabetes and causes various problems in daily life. The aim of this study was to assess the effect of regional anaesthesia on post surgery opioid induced hyperalgesia in diabetic and non-diabetic mice. Methods: Diabetic and non-diabetic mice underwent plantar surgery. Levobupivacaine and sufentanil were used before surgery, for sciatic nerve block (regional anaesthesia) and analgesia, respectively. Diabetic and non-diabetic groups were each randomly assigned to three subgroups: control, no sufentanil and no levobupivacaine; sufentanil and no levobupivacaine; sufentanil and levobupivacaine. Three tests were used to assess pain behaviour: mechanical nociception; thermal nociception and guarding behaviours using a pain scale. Results: Sufentanil, alone or in combination with levobupivacaine, produced antinociceptive effects shortly after administration. Subsequently, sufentanil induced hyperalgesia in diabetic and non-diabetic mice. Opioid-induced hyperalgesia was enhanced in diabetic mice. Levobupivacaine associated to sufentanil completely prevented hyperalgesia in both groups of mice. Conclusion: The results suggest that regional anaesthesia can decrease opioid-induced hyperalgesia in diabetic as well as in non-diabetic mice. These observations may be clinically relevant for the management of diabetic patients. © 2015 Gomez-Brouchet et al.


Franchet C.,Service de medecine legale | Savall F.,Service de medecine legale | Guilbeau-Frugier C.,Service danatomie pathologique et histologie cytologie | Dedouit F.,Service de medecine legale | And 3 more authors.
Revue de Medecine Legale | Year: 2013

Electrocution is an uncommon cause of death. Few articles describe histopathological findings in deaths by electrocution. We report two cases of electrocution, which appeared different for both clinical aspects and final conclusions. For each case, autopsical and histopathological findings are described. Furthermore, the contribution of histological results to the medico-legal purpose is discussed. First case concerned a woman who was found lying near a 380. volts bare electric cable. The autopsy found pulmonary lesions suggesting asphyxia and several skin burns consistent with electrical burns. Microscopic examination confirmed mechanical asphyxia syndrome and observed signs of cutaneous burns with skin metallization. The skin metallization confirmed direct contact with the electric cable. The mechanical asphyxia syndrome was suggestive of respiratory spasms in the context of a "blue electrocuted" The second case concerned a young man whose ladder fell against a power line carrying 20,000. volts. He was given emergency treatment, and then was sent to the hospital, where he died. The autopsy found electric current marks on the upper and the lower limbs. The pathological examination found an ischemic and hemorrhagic aspect of the heart with features of ventricular fibrillation. In both cases, the histopathological examination provided some information, which, added to the accident investigation data and the autopsy findings, permitted to propose some hypothesis about mechanism of death by electrocution shock. © 2013 Elsevier Masson SAS.


Foussal C.,French Institute of Health and Medical Research | Foussal C.,Institut Universitaire de France | Lairez O.,French Institute of Health and Medical Research | Calise D.,British Petroleum | And 9 more authors.
FEBS Letters | Year: 2010

Adipose tissue secretes a variety of bioactive factors, which can regulate cardiomyocyte hypertrophy via reactive oxygen species (ROS). In the present study we investigated whether apelin affects ROS-dependent cardiac hypertrophy. In cardiomyocytes apelin inhibited the hypertrophic response to 5-HT and oxidative stress induced by 5-HT- or H2O2 in a dose-dependent manner. These effects were concomitant to the increase in mRNA expression and activity of catalase. Chronic treatment of mice with apelin attenuated pressure-overload-induced left ventricular hypertrophy. The prevention of hypertrophy by apelin was associated with increased myocardial catalase activity and decreased plasma lipid hydroperoxide, as an index of oxidative stress. These results show that apelin behaves as a catalase activator and prevents cardiac ROS-dependent hypertrophy. © 2010 Federation of European Biochemical Societies.

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