Thiers-sur-Thève, France
Thiers-sur-Thève, France

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Mameri H.,French National Institute for Agricultural Research | Brossard C.,French National Institute for Agricultural Research | Gaudin J.-C.,French National Institute for Agricultural Research | Gohon Y.,French National Institute for Agricultural Research | And 10 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

Wheat products cause IgE-mediated allergies. The present study aimed to decipher the molecular basis of α- and γ-gliadin allergenicity. Gliadins and their domains, the repetitive N-terminal and the nonrepetitive C-terminal domains, were cloned and expressed in Escherichia coli. Their secondary structures and their IgE binding capacity were compared with those of natural proteins before and after reduction/alkylation. Allergenicity was evaluated with sera from patients who had a wheat food allergy or baker's asthma. The secondary structures of natural and recombinant proteins were slightly different. Compared with natural gliadins, recombinant proteins retained IgE binding but with reduced reactivity. Reduction/alkylation decreased IgE binding for both natural and recombinant gliadins. Although more continuous epitopes were identified in the N-terminal domains of α- and γ-gliadins, both the N-terminal and C-terminal domains contributed to IgE binding. As for other members of the prolamin superfamily, disulfide bonds appear to be of high importance for IgE binding. © 2015 American Chemical Society.

Moneret-Vautrin D.-A.,Nancy University Hospital Center | Petit N.,Center hospitalier | Parisot L.,Nancy University Hospital Center | Dumont P.,Nancy University Hospital Center | And 15 more authors.
Revue Francaise d'Allergologie | Year: 2010

Background: Peanut allergy (PA) is persistent and severe, so that therapeutical trials using oral immunotherapy (OIT) are in the spotlight. The primary objective of this pilot study conducted from 2007 to 2009 is the evaluation of the efficiency and the safety of this procedure. Methods: Fifty-one subjects aged 2 to 20 years with persistent PA documented by DBPCFC are enrolled. Doses of roasted peanut powder are weighted in the hospital. The duration of the escalation phase is 17 or 34 weeks according to the severity of PA and the threshold of reactivity. The final dose is 12 grams per week. Controls at specified times are carried out by prick-test and specific IgE to peanut. Adverse reactions (AR) are studied and risk factors are searched out. Results: Forty-eight subjects completed the escalation phase. The duration was extended in two cases. 18 patients have been controlled after six months of the maintenance phase. The global efficiency is estimated at 92.1%. A decrease of prick-tests and an increase of specific IgEs are observed at the end of the escalation phase and evolve conversely six months later. AR occur during both phases, benign in 84% of cases, serious in 16%. They characterize 36.8% patients of group 1, 53.1% of patients in group 2 (0.26% and 0.48% of doses respectively). The relationship of RI with a lower basal threshold is confirmed: 763 ± 363 mg versus 1988 ± 1995 mg (p<0.05). Risk factors are aspirin, exercise sunbathe, viral gastroenteritis, pollinosis, discontinuation of daily doses, intake in decubitus. Eighty-eight percent of RI remain still unpredictable. Conclusions: These results show a benefit of OIT and lead to propose further trials. The duration of the daily dose maintenance and the optimal dose have to be monitored by the immunological profile of IgEs and IgG4s to recombinant peanut major allergens. © 2010 Elsevier Masson SAS.

Moneret-Vautrin D.-A.,University of Lorraine | Vitte J.,Laboratoire dimmunologie | Vitte J.,Aix - Marseille University | Vitte J.,Laboratoire Genclis | And 11 more authors.
Revue Francaise d'Allergologie | Year: 2011

Specific IgE-reactivities have first been implemented by the use of allergenic extracts then purified or recombinant major allergens. The frequent cross-reactivity (CR) due to vegetal and insect carbohydrate determinants has been suppressed by the use of bacteria originated recombinant allergens. CR linked to homology of amino acid sequences leads to classification of allergens, either species specific or cross-reactive ones. The micro array technology (ISAC) makes a component-resolved diagnosis possible by the analysis of 103 allergens. The assessment of this technique may be applied to different pathologies. Negative tests in eight cases of idiopathic anaphylaxis are reported. The ISAC microassay applied to atopic dermatitis shows different polysensitization profiles in 22 children compared to adults. The extensive association of aeroallergen and food allergen sensitizations is a hallmark of eosinophilic esophagitis. Another indication is to set a patient-taylored immunotherapy to pollens and mites. Present restrictions are the CR without clinical relevance (PR-10 family and tropomyosins), the poor performance of certain allergens (wheat allergens, Ana c 2, Ana o 3), the absence of allergens from the following allergenic sources: mustard, lupine, lentils, almond, walnut, buckwheat. In the near future, ISAC could be applied to epidemiological studies and to the follow-up of immunotherapies studied by specific IgG4s. Present prospects are to conduct thorough investigations about the efficiency of the currently available allergens, and to develop computerized algorithms taking into account clinical profiles and patterns of sensitization to improve the diagnosis of clinically relevant sensitization and to achieve the prediction of persistence and severity. © 2011 Elsevier Masson SAS.

Demoly P.,Academie Nationale de Medecine | Mertes M.,French Institute of Health and Medical Research | Moneret-Vautrin A.-D.,University of Lorraine | Michel F.-B.,Service dAllergologie
Bulletin de l'Academie Nationale de Medecine | Year: 2011

Drug allergies are heterogeneous and multifactorial. They can be life-threatening and lead to market withdrawal of particularly risky drugs. Immunological research has improved our understanding of drug allergies but has not yet been able to prevent them. In contrast, epidemiological research not only provides information on the incidence and risk factors of such reactions, but can now, in conjunction the most recent immunological data, also help to prevent some of them. For example, prior pholcodine consumption has been shown to increase the risk of anaphylaxis due to neuromuscular blocking agents during general anesthesia. This review examines the place of interventional epidemiology in the field of drug allergy, taking as examples both myorelaxant-induced anaphylaxis and abacavirinduced DRESS.

Castelain-Hacquet C.,Service dallergologie | Anton M.,Nantes University Hospital Center | Bocquel N.,Pole mere enfant | Cordebar V.,Nancy University Hospital Center | And 8 more authors.
Revue Francaise d'Allergologie | Year: 2011

Severe food allergies, sometimes involving the vital forecast of the patients, are frequent and often carry away consultations and hospitalizations. Treatments according to the indications are still often the allergen eviction to avoid the accidents or the administration of the oral tolerance protocols, which require information and skill on behalf of the patients and of their family. The Research group in therapeutically education in food allergy (Groupe de recherche en éducation thérapeutique dans l'allergie alimentaire), in the light of the recommendations of the French Health Authorities (HAS) in patients therapeutically education, has written the reference table of skills to be acquired by the patients to train in the educational tools. It would specify the way of creating and using them, in connection with the objectives of the reference table of skills. They were estimated and validated, with the aim of harmonizing the practices in this domain; these tools should arise to the largest number of teams interested in the therapeutic education in food allergy. © 2011.

Omidi A.,Service dophtalmologie | Sauvage C.,Service dallergologie | Vandezande L.M.,Service de pneumo allergologie | Wallaert B.,Service de pneumologie et dimmuno allergologie
Revue Francaise d'Allergologie | Year: 2015

Cannabis is a cannabaceae family plant from Central Asia that is now cultivated around the world. It is a low priced, soft drug, readily accessible in some countries because of legislation, in spite of the effects it can cause. Here we describe 5 cases of cannabis LTP (Can s3) sensitization which began before food LTP allergy. Skin tests and serum IgE assays revealed an LTP profile for all the patients. Homologies between cannabis LTP and other LTPs have been described, which suggests that cross reactivity can exist between these LTPs. We suspect that two pathways for cannabis sensitization exist in our patients: a respiratory pathway for all of them and as well as a cutaneous pathway for the three who presented with contact urticaria. As others have reported, cannabis LTP sensitization can be responsible for subsequent food allergy. We propose that food LTP allergy can develop in individuals who have been previously sensitized to cannabis LTP through a cutaneous pathway. © 2015.

Castelain-Hacquet C.,Service dallergologie
Revue Francaise d'Allergologie | Year: 2010

The perspective of taking an airplane when one has food allergies can lead to reflexion and worry in a good number of patients. There are currently few published data on this question and it is difficult to obtain reliable statistics from airline companies. The most recent studies carried out using standardized questionnaires have found that the majority of reactions are due to peanuts, tree-nuts and seeds. Most often, patients treat themselves without alerting the cabin crew. Emergency evacuations are exceptional. Moreover, the quality of the information given to passengers with allergies by customer service departments is very patchy, often incomplete and erroneous. The contents of the on-board emergency kit deserve to be standardized; also, the patient is often not allowed to carry his adrenalin kit on board. © 2010 Elsevier Masson SAS. All rights reserved.

In the 1980s, hypersensitivity to food additives and their adverse effects on health were widely publicized. Today, in spite of its low prevalence, about 1%, it still exists. After a review of some epidemiologic data and a short history of regulatory mechanisms in Europe and USA, additives can be separated into three groups to facilitate diagnosis: IgE-dependent (cochineal, erythrosine, red rice, annatto, carrageenans, adragant gums, lyzozyme), additives confirmed by DBPCFC (tartrazine, sulfites, benzoates, nitrites, diacetyl, fragrances), and disputed substances (monosodium glutamate, aspartame). Allergists are encouraged to rigorously investigate different food categories, using prick-tests correlated with IgE levels and confirmation by DBPCFC when continuing to look for the cause of these reactions. © 2010 Elsevier Masson SAS. All rights reserved.

Vilain A.-C.,Service dallergologie
Revue Francaise d'Allergologie | Year: 2010

Milk is produced by the mammary glands of mammals. It provides the primary source of nutrition for young mammals. It is rich in proteins and carbohydrates. Numerous dairy products are made from milk. Its components are used as ingredients in industrial products. Cow milk is an allergen which must be declared on labels. © 2010 Elsevier Masson SAS. All rights reserved.

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