SERVA Electrophoresis GmbH

Heidelberg, Germany

SERVA Electrophoresis GmbH

Heidelberg, Germany
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Market Research Report on Epoxy Propane market 2016 is a professional and in-depth study on the current state of the Epoxy Propane worldwide. First of all,"Global Epoxy Propane Market 2016" report provides a basic overview of the Epoxy Propane industry including definitions, classifications, applications and Epoxy Propane industry chain structure. Global Epoxy Propane Industry Trends, Share, Research, Forecast, Demand, Gross Margin, Growth Analysis, Revenue, Cost, Supply, Major Regions Status and R&D Status. The analysis is provided for the Epoxy Propane international market including development history, Epoxy Propane industry competitive landscape analysis.  This report "Worldwide Epoxy Propane Market 2016" also states import/export, supply and consumption figures and Epoxy Propane market cost, price, revenue and Epoxy Propane market's gross margin by regions (United States, EU, China and Japan), as well as other regions can be added in Epoxy Propane Market area. Major Manufacturers are covered in this research report are Acros Organics  Riedel-de Haen AG Cambridge Isotope Laboratories SIGMA-RBI DuPont Specialty Colorants & Additives SERVA Electrophoresis GmbH  Dow Chemical Company Sumitomo Chemical Singapore Pte Ltd  Shell Nederland Chemie B.V. BASF Corporation This report studies Epoxy Propane in Global market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top manufacturers in global market, with sales, price, revenue and market share. Then, the report focuses on worldwide Epoxy Propane market key players with information such as company profiles with product picture as well as specification. Related information to Epoxy Propane market- capacity, production, price, cost, revenue and contact information. Aslo includes Epoxy Propane industry's - Upstream raw materials, equipment and downstream consumers analysis is also carried out. What’s more, the Epoxy Propane market development trends and Epoxy Propane industry marketing channels are analyzed. Finally, "Worldwide Epoxy Propane Market" Analysis- feasibility of new investment projects is assessed, and overall research conclusions are offered.


Griebel A.,SERVA Electrophoresis GmbH | Obermaier C.,SERVA Electrophoresis GmbH | Westermeier R.,SERVA Electrophoresis GmbH | Moche M.,University of Greifswald | Buttner K.,University of Greifswald
Archives of Physiology and Biochemistry | Year: 2013

A new fluorescent amino-reactive dye has been tested for both labelling proteins prior to electrophoretic separations and between the two steps of two-dimensional electrophoresis. A series of experiments showed, that the labelling of lysines with this dye is compatible with all standard additives used for sample preparation, including reducing substances and carrier ampholytes. Using this dye for pre-labelling considerably simplifies the electrophoresis and detection workflow and provides highly sensitive and quantitative visualisation of proteins. © 2013 Informa UK Ltd.


Griebel A.,SERVA Electrophoresis GmbH | Obermaier C.,SERVA Electrophoresis GmbH | Westermeier R.,SERVA Electrophoresis GmbH | Moche M.,University of Greifswald | Buttner K.,University of Greifswald
BioSpektrum | Year: 2013

Fluorescent pre-labelling of proteins prior to electrophoretic separations is not only advantageous for multiplex detection strategies, like DIGE (difference gel electrophoresis), but also as a general protein visualization procedure. We introduce a fluorescent amino-reactive dye which is compatible with all standard additives used for protein sample preparation including reductants. Fluorescent pre-labelling is less time-consuming and as sensitive as silver staining with additionally excellent quantification properties. © 2013 Springer-Verlag Berlin Heidelberg.


Moche M.,University of Greifswald | Albrecht D.,University of Greifswald | Maass S.,University of Greifswald | Hecker M.,University of Greifswald | And 2 more authors.
Electrophoresis | Year: 2013

A principally new type of an electrophoresis setup for the second dimension of 2DE named HPE (high performance electrophoresis) has recently become available that provides excellent reproducibility much superior to traditional 2DE. It takes up ideas from early beginnings of 2DE which could not be satisfactory realized at that time. The new HPE system is in contrast to all other established systems a horizontal electrophoresis that employs a new type of precast polyacrylamide gels on film-backing and runs on a multilevel flatbed electrophoresis apparatus. In a systematic approach we compared its features to traditional 2DE for the cytosolic proteome of Bacillus subtilis. Not only the reproducibility is enhanced, but also nearly all qualitative parameters as resolution, sensitivity, the number of protein spots (25% more), and the number of different proteins (also additional 25%) are markedly increased. More than 200 proteins were exclusively found in HPE. This new electrophoresis system does not use buffer tanks. No glass plates are needed. Therefore handling of gels is greatly facilitated and very simple to use even for personnel with low technical skills. The new HPE system is technically at the beginnings and further development with increased performance can be expected. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Hanneken M.,University of Munster | Ackermann D.,University of Munster | Konig S.,University of Munster | Thesseling G.,SERVA Electrophoresis GmbH
BioSpektrum | Year: 2014

A major problem in two-dimensional protein gel electrophoresis is the little comparability of gel images. A reference grid of marker proteins in parallel to the separation of the analyte by use of two fluorescent dyes allows the correction of protein coordinates. The deviation from mean is improved by an order of magnitude. The technology called comparative 2D fluorescence gel electrophoresis (CoFGE) can be carried out in both vertical and horizontal electrophoresis instrumentation. © Springer-Verlag 2014.


Since recent years the separation of soluble and membrane protein complexes is thought to be a powerful tool for protein characterizations and assembly studies of high molecular weight complexes.


2D electrophoresis of intact proteins is the central separation method of the top-down approach in proteomics. Hence it is important to exploit all its technical potentials for optimizing separation results and reproducibility.


PubMed | Uppsala University, University of Oxford and Serva Electrophoresis GmbH
Type: Journal Article | Journal: Transplantation direct | Year: 2016

Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI as essential enzymes for human islet isolation.Human islets were isolated using 4 different enzyme mixtures composed of CII plus either intact (CI-115) or degraded CI (CI-100). Blends were administered either with or without NP/CP. Purified islets were cultured for 3 to 4 days before islet quality assessment.Whereas using intact CI-115 without NP/CP did not significantly reduce islet yield (3429 631 vs 3087 970 islet equivalent/g, nonsignificant), administration of degraded CI-100 without NP/CP decreased islet yield from 3501 580 to 1312 244 islet equivalent/g (P < 0.01), doubled the amount of undigested tissue from 11.8 1.6 to 24.4 1.2% (P < 0.01) and triplicated the percentage of trapped islets from 7.7 2.8 to 22.5 3.6% (P < 0.05). Islet yield did not vary between supplemented CI-115 and CI-100, but was increased using CI-115 when NP/CP was omitted (P < 0.05). A trend toward higher viability and increased secretory insulin response was noted in both CI-100 and CI-115 when NP/CP was not added.This study suggests that NP/CP can compensate reduced CI activity. Future attempts to optimize enzyme blends should consider the possibility to increase the proportion of collagenase CI to reduce the need for potentially harmful NPs.


PubMed | SERVA Electrophoresis GmbH
Type: | Journal: Methods in molecular biology (Clifton, N.J.) | Year: 2015

Protein labeling methods prior to separation and analysis have become indispensable approaches for proteomic profiling. Basically, three different types of tags are employed: stable isotopes, mass tags, and fluorophores. While proteins labeled with stable isotopes and mass tags are measured and differentiated by mass spectrometry, fluorescent labels are detected with fluorescence imagers. The major purposes for protein labeling are monitoring of biological processes, reliable quantification of compounds and specific detection of protein modifications and isoforms in multiplexed samples, enhancement of detection sensitivity, and simplification of detection workflows. Proteins can be labeled during cell growth by incorporation of amino acids containing different isotopes, or in biological fluids, cells or tissue samples by attaching specific groups to the -amino group of lysine, the N-terminus, or the cysteine residues. The principles and the modifications of the different labeling approaches on the protein level are described; benefits and shortcomings of the methods are discussed.

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