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Sun S.-H.,ShenYang Agricultural University | Li Y.-P.,ShenYang Agricultural University | Zheng Y.-N.,ShenYang Agricultural University | Xu X.-R.,ShenYang Agricultural University | And 5 more authors.
Annals of the Entomological Society of America | Year: 2011

Selenophosphate synthetase (Sps), the product of the SelD gene, produces a biologically active selenium donor compound from ATP and selenide. We have isolated and characterized the Sps gene from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae), an economically important insect. The resulting 1601 bp cDNA sequence contains an open reading frame of 1209 bp encoding a polypeptide of 402 amino acids, with 87% sequence identity to that from Drosophila melanogaster (Meigen). Semiquantitative reverse transcription-polymerase chain reaction (PCR) analysis showed that the Sps gene was transcribed during four developmental stages (egg, larva, pupa, and adult) and in all the tissues tested (blood, fat body, midgut, silk glands, body wall, spermaries and ovaries), suggesting that ApSps plays an important role in the development of A. pernyi. From a database search, Sps protein homologs were found in prokaryotes and eukaryotes, including bacteria, fungi, invertebrates and vertebrates, with 47-98% amino acid sequence identities between eukaryotes, suggesting that they were highly conserved during the evolution of eukaryotes. Phylogenetic analysis, based on Sps protein homolog sequences, clearly separated the known bacterial, fungal, invertebrate and vertebrate Sps proteins, consistent with the topology tree of classical systematics, suggesting the potential value of the Sps protein sequence in phylogenetic inference. © 2011 Entomological Society of America. Source

Liu Y.,ShenYang Agricultural University | Li Y.,ShenYang Agricultural University | Li X.,Sericultural Institute of Liaoning Province | Qin L.,ShenYang Agricultural University
Journal of Insect Science | Year: 2010

Sericulture is one of the great inventions of the ancient Chinese. Besides the mulberry silkworm (Bombyx mori), Chinese farmers developed rearing of the Chinese oak silkworm (Antheraea pernyi) about 400 years ago. In this paper, the historic records of the origins and dispersal of the domesticated Chinese oak silkworm in China are summarized. The first document with clearly recorded oak silkworm artificial rearing appeared in 1651, although Chinese oak silkworm was documented in about 270 AD. All of the evidence in the available historic records suggests that the domesticated Chinese oak silkworm originated in central and southern areas of Shandong Province in China around the 16th century, and then was introduced directly and indirectly by human commerce into the present habitations in China after the late 17th century. The results strongly support the hypothesis that only one geographically distinct event occurred in domestication of the modern Chinese oak silkworm. Source

Chen M.-M.,ShenYang Agricultural University | Li Y.,ShenYang Agricultural University | Chen M.,ShenYang Agricultural University | Wang H.,ShenYang Agricultural University | And 7 more authors.
Gene | Year: 2014

Mitochondrial genome (mitogenome) can provide information for genomic structure as well as for phylogenetic analysis and evolutionary biology. In this study, we present the complete mitogenome of the atlas moth, Attacus atlas (Lepidoptera: Saturniidae), a well-known silk-producing and ornamental insect with the largest wing surface area of all moths. The mitogenome of A. atlas is a circular molecule of 15,282. bp long, and its nucleotide composition shows heavily biased towards As and Ts, accounting for 79.30%. This genome comprises 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an A. +. T-rich region. It is of note that this genome exhibits a slightly positive AT skew, which is different from the other known Saturniidae species. All PCGs are initiated by ATN codons, except for COI with CGA instead. Only six PCGs use a common stop codon of TAA or TAG, whereas the remaining seven use an incomplete termination codon T or TA. All tRNAs have the typical clover-leaf structure, with an exception for tRNASer(AGN). The A. atlas A. +. T-rich region contains non-repetitive sequences, but harbors several features common to the Bombycoidea insects. The phylogenetic relationships based on Maximum Likelihood method provide a well-supported outline of Saturniidae, which is in accordance with the traditional morphological classification and recent molecular works. © 2014 Elsevier B.V. Source

Chen M.,ShenYang Agricultural University | Chen M.-M.,ShenYang Agricultural University | Yao R.,ShenYang Agricultural University | Li Y.,ShenYang Agricultural University | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi, an important agricultural and edible insect, designated ApFKBP12 A and B, respectively. Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity. Phylogenetic analysis showed that FKBP12 B sequences of A. pernyi, Bombyx mori, and Danaus plexippus were clearly separated from FKBP12 A sequences of these three species, suggesting that insect FKBP12 A and B may have been evolving independently. RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues, and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than that of the ApFKBP12 B gene. After heat shock treatment, expressions of the two FKBP12 genes were up-regulated, but at different time points. The results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A. pernyi. © 2013 American Chemical Society. Source

Jiang D.-F.,Sericultural Institute of Liaoning Province | Jiang D.-F.,ShenYang Agricultural University | Liu Y.-Q.,Sericultural Institute of Liaoning Province | Liu Y.-Q.,ShenYang Agricultural University | And 2 more authors.
African Journal of Biotechnology | Year: 2010

It has been known that the abnormal wing disc (awd) gene encodes a nucleoside diphosphate kinase and is closely related to wing development in Drosophila melanogaster and Bombyx mori. In the present study, the awd gene was isolated and characterized from Antheraea pernyi, a well-known wild silkmoth. The isolated cDNA sequence is 666 bp in length with an open reading frame of 462 bp encoding a polypeptide of 153 amino acids, which contains a putative nucleoside diphosphate kinases active site motif and conserved multimer interface. The deduced A. pernyi awd protein sequence reveals 75, 82 and 96% identity with its homologue of Homo sapiens, D. melanogaster, and B. mori, respectively. Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that the awd gene was transcribed during all four developmental stages (egg, larva, pupa, and moth), and present in all tissues tested (blood, midgut, silk glands, Malpighian tublues, spermaries, ovaries, brain, muscle, fat body and body wall), with the highest abundance in Malpighian tubules. Interestingly, mRNA expression level in pupal fat body was significantly down-regulated after cold shock (4°C) compared with the control (26°C) and significantly up-regulated after heat shock (46°C). The results indicated that the A. pernyi awd gene is inducible, and that its expression effect is different after cold stress and heat stress. Consequently, we refer that the product of the awd gene may contribute to its temperature tolerance. © 2010 Academic Journals. Source

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