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Aleksic D.,Mother and Child Health Care Institute | Djokic D.,Mother and Child Health Care Institute | Golubicic I.,Serbian Institute for Oncology and Radiology of Serbia | Jakovljevic V.,University of Kragujevac | Djuric D.,Serbian Institute of Physiology
Journal of B.U.ON. | Year: 2013

Purpose: Hyperhomocysteinemia is associated with carcino genesis. Since only little research exists on hyperhomocysteinemia and malignancy in children, the possible relationship between homocysteine and childhood malignancies remains unknown. The aim of the present study was to determine the serum levels of homocysteine, folic acid and vitamin 1312 in children with malignant and benign tumors prior to therapy (surgical treatment and/or chemotherapy), and after treatment of malignant diseases as well. Methods: Forty-six children with newly diagnosed malignant diseases (solid tumors and lymphoprolferative/ myeloproliferative (LP/MP) malignancies) and 6 children with benign tumors were included in the present study. The patient age ranged between 2 months and 18 years. Results: Significantly increased homocysteine concentrations were identified in children with malignant diseases compared with those with benign tumors (pO.O5). The concentration of vitamin B12 in children with malignant diseases (solid tumors and LP/MP diseases) increased significantly following treatment (p


Vujovic T.,Serbian Institute of Physiology | Ruzic Dj.,Serbian Institute of Physiology | Cerovic R.,Maize Research Institute Zemun Polje
Horticultural Science | Year: 2012

In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes, The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.


Veselinovic M.V.,Clinical Center Kragujevac | Toncev S.,Clinical Center Kragujevac | Tasic N.,Dedinje Institute for Cardiovascular Disease | Bogdanovic V.,Health Center Zvezdara | Djuric D.M.,Serbian Institute of Physiology
Vasa - Journal of Vascular Diseases | Year: 2012

Background: Cardiovascular (CV) morbidity and mortality are increased in patients with rheumatoid arthritis (RA). Our study aim was to determine the relationship between carotid artery intima-media wall thickness (IMT) and flowmediated endothelium-dependent vasodilatation (FMD) in a patients with RA, in context with clinical and laboratory measurements. Patients and methods: Fift y-two patients with RA and 30 matched healthy controls without clinically evident CV disease were studied. Brachial and carotid ultrasonography was performed to determine FMD and IMT, respectively. We also assayed immunological, inflammatory and metabolic laboratory markers. Results: IMT was significantly higher in RA patients (1.00 ± 0.16 mm) patients than in controls (0.89 ± 0.13 mm) (P = 0.001). FMD was significantly lower in RA (9.16 ± 7.03) as compared to controls (12.60 ± 5.49) (p = 0.005). RA patients had significant positive correlations between erythrocyte sedimentation rate (ESR) (r=0.395 p = 0.021) and IMT and negative correlation between visual analog scale (VAS) (r= -0.311, p= 0.025) and IMT. RA patients who used low doses of corticosteroids have, statistically, significantly better FMD, than those who do not use corticosteroids. Linear regression analysis revealed that IMT was related to tender joint count (p = 0.008), VAS (p < 0.001), ESR (p = 0.048) and total cholesterol/ high density lipoprotein cholesterol ratio (p = 0.039). Conclusions: In patients with RA, FMD was impaired and IMT was increased, indicating early endothelial dysfunction and accelerated atherosclerosis. Early treatment of disease may reduce the risk of atherosclerosis in RA. © 2012 Hans Huber Publishers, Hogrefe AG, Bern.


Omar K.,Aristotle University of Thessaloniki | Geronikaki A.,Aristotle University of Thessaloniki | Zoumpoulakis P.,National Hellenic Research Foundation | Sokovic M.,Serbian Institute of Physiology | And 2 more authors.
Bioorganic and Medicinal Chemistry | Year: 2010

As part of ongoing studies in developing new antimicrobials, a class of structurally novel 4-thiazolidinone derivatives incorporating three known bioactive nuclei such as thiazole, thiazolidinone and adamantane was synthesized by the multi-step reaction protocol, already reported in the literature. NMR and Molecular Modeling techniques were employed for structure elucidation and Z/E potential isomerism configuration of the analogues. Evaluation of antibacterial and antifungal activity showed that almost all compounds exhibited better results than reference drugs thus they could be promising candidates for novel drugs. © 2009 Elsevier Ltd. All rights reserved.


Camoutsis C.,University of Patras | Geronikaki A.,Aristotle University of Thessaloniki | Ciric A.,Serbian Institute of Physiology | Sokovic M.,Serbian Institute of Physiology | And 2 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2010

A series of thirteen new thiadiazole compounds were synthesized and evaluated for in vitro antifungal and antibacterial activity. All compound tested showed significant antifungal activity against all the micromycetes, compared to the commercial fungicide bifonazole. Differences in their activity depend on the substitution of different reactive groups. More specifically, best antifungal activity was shown for the synthetic analogue with methylpiperazine reactive group. Furthermore, it is apparent that different compounds reacted on different ways against bacteria. An effort was made to correlate the above mentioned differences in activity with lipophilicity studies. Furthermore, NMR and molecular modelling were used to obtain the main conformational features of a potent analogue, for future in silico studies. © 2010 Pharmaceutical Society of Japan.


Prasad P.V.V.,Kansas State University | Pisipati S.R.,Kansas State University | Momcilovic I.,Kansas State University | Momcilovic I.,Serbian Institute of Physiology | Ristic Z.,Plant Science and Entomology Research Unit
Journal of Agronomy and Crop Science | Year: 2011

High temperature and drought stress are among the two most important environmental factors influencing crop growth, development and yield processes. These two stresses commonly occur in combination. Objectives of this research were to investigate the independent and combined effects of high temperature and drought stress during grain filling on physiological, vegetative and yield traits and expression of a chloroplast protein synthesis elongation factor (EF-Tu) of wheat (Triticum aestivum L.). Two spring wheat cultivars (Pavon-76 and Seri-82) were grown at control temperatures (CT; day/night, 24/14°C; 16/8h photo/dark period) from sowing to heading. Thereafter, one half of the plants were exposed to high temperature stress (HT; 31/18°C in Exp. 1 and 34/22°C in Exp. 2), drought stress (withholding water), or a combination of both HT and drought stress. There were significant influences of HT and/or drought stress on physiological, growth and yield traits. There was no cultivar or cultivar by temperature or cultivar by drought interaction effects on most traits. The decreases in leaf photosynthesis were greater at HT compared with drought alone throughout the stress period, and the combination of HT and drought had the lowest leaf photosynthetic rates. Overall, HT or drought had similar effects (about 48-56% decrease) on spikelet fertility, grain numbers and grain yield. High temperature decreased grain numbers (by 56% averaged across both experiments) and individual grain weight (by 25%), while, respective decreases due to drought were 48% and 35%. This suggests that the grain numbers were more sensitive to HT and grain weights to drought for the range of temperatures tested in this research. The interaction between HT and drought stress was significant for total dry weights, harvest index and spikelet fertility, particularly when HT stress was severe (34/22°C). The combined effects of HT and drought were greater than additive effects of HT or drought alone for leaf chlorophyll content, grain numbers and harvest index. High temperature stress and the combination of HT and drought stress but not drought stress alone resulted in the overexpression of EF-Tu in both spring wheat cultivars. © 2011 Blackwell Verlag GmbH.


Jakovljevic V.Lj.,University of Kragujevac | Djordjevic D.Z.,University of Kragujevac | Djuric D.M.,Serbian Institute of Physiology
General Physiology and Biophysics | Year: 2011

The aim of this study was to assess the effects of vitamin C (ascorbic acid) on coronary flow and oxidative stress markers with or without non-specific inhibition of nitric oxide synthase by N ω-nitro-L-arginine monomethyl ester (L-NAME) in isolated rat hearts. The hearts of male Wistar albino rats (n = 12, age 8 weeks, body mass 180-200 g) were retrograde perfused according to the Langendorff technique at gradually increased constant perfusion pressure (40-120 cm H 2O). Coronary flow, nitrite outflow, superoxide anion production, and index of lipid peroxidation (by measuring thiobarbituric acid reactive substances) in coronary effluent were determined. The experiments were performed during control conditions and in presence of vitamin C (100 μM) alone or vitamin C (100 μM) + L-NAME (30 μM). Administration of vitamin C induced only increase of nitrite levels, while vitamin C + L-NAME induced significant decrease of coronary flow above autoregulatory range, i.e. especially at higher coronary perfusion pressure (CPP) values, accompanied with similar dynamic in nitrite outflow. Vitamin C + L-NAME also induced significant decrease in TBARS production. The results of our study show no significant effects of vitamin C administration either on ROS levels or on coronary flow in isolated rat heart.


Veselinovic M.,Clinical Center Kragujevac | Barudzic N.,University of Kragujevac | Vuletic M.,University of Kragujevac | Zivkovic V.,University of Kragujevac | And 3 more authors.
Molecular and Cellular Biochemistry | Year: 2014

The aim of this study was to assess the oxidative stress status in rheumatoid arthritis (RA) by measuring markers of free radical production, systemic activity of disease, and levels of antioxidant. 52 RA patients and 30 healthy controls were included in the study, and clinical examination and investigations were performed and disease activity was assessed. Peripheral blood samples were used for all the assays. We assessed the markers of oxidative stress, including plasma levels of index of lipid peroxidation-thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O 2), superoxide anion radical (O2 -), nitric oxide (NO), and superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione levels in erythrocytes. In the RA group, levels of H 2O2, O2 -, and TBARS were significantly higher than in controls (4.08 ± 0.31 vs. 2.39 ± 0.13 nmol/l, p < 0.01; 8.90 ± 1.28 vs. 3.04 ± 0.38 nmol/l, p < 0.01, 3.65 ± 0.55 vs. 1.06 ± 0.17 μmol/l, p < 0.01). RA patients had significantly increased SOD activity compared with healthy controls (2,918.24 ± 477.14 vs. 643.46 ± 200.63UgHbx103, p < 0.001). Patients had significantly higher levels of pro-oxidants (O2 -, H2O2, and TBARS) compared to controls, despite significantly higher levels of SOD. Significant differences were also observed in serum levels of NO in patients with high-diseases activity. Our findings support an association between oxidative/nitrosative stress and RA. Stronger response in samples with higher diseases activity suggests that oxidative/nitrosative stress markers may be useful in evaluating the progression of RA as well as in elucidating the mechanisms of disease pathogenesis. © 2014 Springer Science+Business Media.


Djordjevic D.,University of Kragujevac | Cubrilo D.,University of Kragujevac | MacUra M.,University of Belgrade | Barudzic N.,University of Kragujevac | And 2 more authors.
Molecular and Cellular Biochemistry | Year: 2011

Although exercise-induced oxidative stress receives considerable scientific attention, there is still little information available regarding exercise-induced adaptations of the antioxidant defence system in adolescent and child athletes. The aim of our study was to establish the effects of long-term exercise training on the redox state of adolescents, and to find correlations between elements of redox homeostasis and aerobic power. Thirty-three handball players and 14 non-athletes, 16-19-years old, were subjected to blood sampling to measure levels of nitric oxide (NO; estimated through nitrites (NO 2 -)), superoxide anion radical (O2 -), hydrogen peroxide (H2O2), lipid peroxidation (estimated through TBARS), superoxide dismutase (SOD) and catalase (CAT). Subjects were also subjected to maximal progressive exercise test to estimate their maximal oxygen consumption ( $$ {{{\text{VO}}-{ 2} { \max }}} $$ ). Athletes had significantly (P < 0.05) higher SOD activity and lower CAT activity compared with non-athletes (SOD: 2175.52 ± 362.07 compared with 1172.16 ± 747.40 U/g of hemoglobin × 10 , 3 and CAT: 2.19 ± 0.31 compared with 3.08 ± 0.47 U/g of hemoglobin × 103). These differences were the most obvious when comparing non-athletes and athletes with poor/average aerobic power. H2O2 and TBARS levels differed among subjects with poor, average or good aerobic power (P < 0.01, and P < 0.05, respectively). Sports engagament and aerobic capacity are important factors in inducing changes in redox status. © 2011 Springer Science+Business Media, LLC.


PubMed | University of Sfax, Aristotle University of Thessaloniki, Serbian Institute of Physiology, CHC Bezanijska kosa and 2 more.
Type: Journal Article | Journal: Hormones (Athens, Greece) | Year: 2016

There is a need for a simple and accurate method for the assessment of cardiovascular risk in polycystic ovary syndrome (PCOS). Lipid accumulation product (LAP) is based on the assessment of waist circumference and serum triglycerides that yield an estimation of lipid overaccumulation. We aimed to determine whether LAP is associated with metabolic syndrome (MetS) in Caucasian women with PCOS.We studied 222 women with PCOS who were diagnosed using the Rotterdam criteria. In all the subjects and controls, LAP was determined and the MetS was assessed using three different international criteria, NCEP-ATP III, IDF, and JIS. ROC curve and logistic regression analyses were performed to determine and analyze associations with the MetS.In the study population the prevalence of MetS was 16.2-19.4%. The cut-off value of 25.9 determined that LAP has the strongest association with MetS whichever international criteria are used, followed by HDL (NCEP-ATP III and JIS) and glucose (IDF).LAP is used as an independent clinical indicator for MetS in our PCOS women of Caucasian origin. The high diagnostic accuracy of LAP is superseding the need for the use of multiple clinical indicators for the assessment of lipid accumulation as a prerequisite for diagnosis of metabolic and cardiovascular diseases in PCOS women.

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