Serbian Institute of Occupational Health Dr Dragomir Karajovic

Belgrade, Serbia

Serbian Institute of Occupational Health Dr Dragomir Karajovic

Belgrade, Serbia

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Pantelic G.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Pantelic G.,Institute of Veterinary Medicine of Serbia | Vuletic V.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Mitrovic R.,Institute of Veterinary Medicine of Serbia
Applied Radiation and Isotopes | Year: 2010

This paper presents the statistical evaluation of results from the analysis of 8 radionuclides in water samples within the frame of the First Proficiency Test of gamma spectrometry Laboratories in Serbia organized in 2008. The water samples used in this proficiency test were prepared using certified radioactive solution containing gamma emitting radionuclides. This solution was diluted and used as a master solution for preparation of test samples. The overall performance evaluation showed that 64.7% of all reported results met the individual proficiency test criteria, where 26.5% of all reported results did not pass the overall PT acceptance criterion. © 2009 Elsevier Ltd.


Pajic J.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Rakic B.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Jovicic D.,Singidunum University | Milovanovic A.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Milovanovic A.,University of Belgrade
Mutation Research - Genetic Toxicology and Environmental Mutagenesis | Year: 2014

Biological dosimetry using chromosome damage biomarkers is a valuable dose assessment method in cases of radiation overexposure with or without physical dosimetry data. In order to estimate dose by biodosimetry, any biological dosimetry service have to have its own dose response calibration curve. This paper reveals the results obtained after irradiation of blood samples from fourteen healthy male and female volunteers in order to establish biodosimetry in Serbia and produce dose response calibration curves for dicentrics and micronuclei. Taking into account pooled data from all the donors, the resultant fitted curve for dicentrics is: Ydic=0.0009 (±0.0003)+0.0421 (±0.0042)×D+0.0602 (±0.0022)×D2; and for micronuclei: Ymn=0.0104 (±0.0015)+0.0824 (±0.0050)×D+0.0189 (±0.0017)×D2. Following establishment of the dose response curve, a validation experiment was carried out with four blood samples. Applied and estimated doses were in good agreement. On this basis, the results reported here give us confidence to apply both calibration curves for future biological dosimetry requirements in Serbia. © 2014 Elsevier B.V.


Pajic J.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Rakic B.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Jovicic D.,Singidunum University | Milovanovic A.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Milovanovic A.,University of Belgrade
International Archives of Occupational and Environmental Health | Year: 2016

Purpose: The health risk of chronic exposure to radionuclides includes changes in the genome (e.g., chromosomal aberrations and micronuclei) that increase chromosomal instability. There are also other phenomena, which seem to appear more frequently in metaphases of exposed persons (such as premature centromere division). The aim of this study was to discover whether or not there is correlation between incidence of named cytogenetic changes in persons occupationally exposed to radionuclides in comparison with unexposed control group, and if significant correlation is determined, can premature centromere division be consider as a biomarker of radiation exposure? Methods: The exposed group comprised 50 individuals occupationally exposed to radionuclides. The reference control group consisted of 40 unexposed individuals. Chromosomal aberrations, micronuclei and premature centromere division were analyzed according to a standard International Atomic Energy Agency protocol. Statistical analyses were performed using SPSS 17.0 statistics. Results: The means for analyzed cytogenetic changes were significantly higher in the exposed group. Positive correlation between them was found in exposed group. Premature centromere division parameter PCD5-10 was selected as particularly suitable for separating groups (exposed/unexposed). Conclusions: Identification of other phenomena related to radionuclide exposure, beside well known, may clarify recent problems in radiobiology concerning the biological response to low doses of ionizing radiation and its consequences. © 2015, Springer-Verlag Berlin Heidelberg.


Pajic J.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Jovicic D.,Singidunum University | PS Milovanovic A.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | PS Milovanovic A.,University of Belgrade
Biomarkers | Year: 2016

Context: Genotoxicity assays are widely employed in human biomonitoring studies to assess genetic damage inflicted by genotoxic agents. Objective: Evaluation of micronuclei (MN) as a screening marker of occupational ionizing radiation (IR) exposure. Materials and methods: Using micronucleus test, peripheral blood lymphocytes (PBL) of 402 control and exposed subjects were screened for genetic damage. Results: The mean frequencies of micronucleus test parameters were significantly higher in exposed persons. Increase of micronucleus yield with duration of exposure (DOE) by 0.303MN/year was revealed. Discussion and conclusion: The obtained data encourage us to consider MN as valuable markers for preventive medical screening of occupationally exposed groups. © 2016 Informa UK Limited, trading as Taylor & Francis Group


PubMed | University of Belgrade, Singidunum University and Serbian Institute of Occupational Health Dr Dragomir Karajovic
Type: | Journal: Mutation research. Genetic toxicology and environmental mutagenesis | Year: 2014

Biological dosimetry using chromosome damage biomarkers is a valuable dose assessment method in cases of radiation overexposure with or without physical dosimetry data. In order to estimate dose by biodosimetry, any biological dosimetry service have to have its own dose response calibration curve. This paper reveals the results obtained after irradiation of blood samples from fourteen healthy male and female volunteers in order to establish biodosimetry in Serbia and produce dose response calibration curves for dicentrics and micronuclei. Taking into account pooled data from all the donors, the resultant fitted curve for dicentrics is: Ydic=0.0009 (0.0003)+0.0421 (0.0042)D+0.0602 (0.0022)D(2); and for micronuclei: Ymn=0.0104 (0.0015)+0.0824 (0.0050)D+0.0189 (0.0017)D(2). Following establishment of the dose response curve, a validation experiment was carried out with four blood samples. Applied and estimated doses were in good agreement. On this basis, the results reported here give us confidence to apply both calibration curves for future biological dosimetry requirements in Serbia.


Pantelic G.,Vinča Institute of Nuclear Sciences | Savkovic M.E.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Zivanovic M.,Vinča Institute of Nuclear Sciences | Nikolic J.,Vinča Institute of Nuclear Sciences | And 2 more authors.
Applied Radiation and Isotopes | Year: 2014

Active charcoal detectors are used for testing the concentration of radon in dwellings. The method of measurement is based on radon adsorption on coal and measurement of gamma radiation of radon daughters. The contributions to the final measurement uncertainty are identified, based on the equation for radon activity concentration calculation. Different methods for setting the region of interest for gamma spectrometry of canisters were discussed and evaluated. The obtained radon activity concentration and uncertainties do not depend on peak area determination method. © 2013 Elsevier Ltd.


Forkapic S.,University of Novi Sad | Bikit K.,University of Novi Sad | Arsic V.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | Ilic J.,Serbian Institute of Occupational Health Dr Dragomir Karajovic | And 2 more authors.
Nukleonika | Year: 2016

Results and conclusions of interlaboratory comparison of indoor radon in 2015 in Serbia are presented. The participants were three accredited laboratories from Serbia: Serbian Institute of Occupational Health "Dr Dragomir Karajovic", Laboratory for Radioactivity and Dose Measurements at the Faculty of Sciences, University of Novi Sad and Radiation and Environmental Protection Department, Vinca Institute of Nuclear Science. The laboratories make use of the same method for radon measurement, using charcoal canisters according to US Environmental Protection Agency (EPA) protocol 520/5-87-005. Calibration of detection efficiency was performed using EPA radium standard. Radon activity concentrations were determined on the basis of the intensity of short-living radon daughters, 214Bi and 214Pb, gamma lines. The results of intercomparison were evaluated by using the u-test, which was calculated according to the International Atomic Energy Agency criteria. In this paper, not only limitations but also the advantages and possibilities of application of this method for measuring levels of human exposure to radon are discussed.


PubMed | Serbian Institute Of Occupational Health Dr Dragomir Karajovic
Type: Journal Article | Journal: International journal of public health | Year: 2012

Middle- and low-income countries rarely have national surveillance data on smoking in pregnancy. This nationwide population-representative survey investigated pre- and post-partum smoking and their predictors in Serbia.Using stratified two-stage random cluster sampling, 2,721 women in 66 health care centres were interviewed at 3 and 6 months post-partum.37.2% of women smoked at some point in pregnancy (average 8.8 cigarettes/per day). Smoking at pregnancy onset and during pregnancy was associated with smoking by others in the home and lower education and family socio-economic status. Almost a quarter of women (23.2%) who quit smoking during pregnancy did not relapse 6 months post-partum. Older women, primiparae, university students and white-collar workers were more likely to successfully quit smoking. More than a half of women were exposed to SHS in their homes (57.6%) and 84.6% allowed smoking in their homes.Smoking during pregnancy in Serbia was two- to threefold higher than in the most affluent western countries. Target groups for action are women with lower education and socio-economic status, as well as health professionals and family members who smoke.


PubMed | Serbian Institute of Occupational Health Dr Dragomir Karajovic
Type: Journal Article | Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine | Year: 2010

This paper presents the statistical evaluation of results from the analysis of 8 radionuclides in water samples within the frame of the First Proficiency Test of gamma spectrometry Laboratories in Serbia organized in 2008. The water samples used in this proficiency test were prepared using certified radioactive solution containing gamma emitting radionuclides. This solution was diluted and used as a master solution for preparation of test samples. The overall performance evaluation showed that 64.7% of all reported results met the individual proficiency test criteria, where 26.5% of all reported results did not pass the overall PT acceptance criterion.


PubMed | Serbian Institute of Occupational Health Dr Dragomir Karajovic
Type: Comparative Study | Journal: Radiation and environmental biophysics | Year: 2015

Ionizing radiation can induce a wide range of DNA damage that leads to chromosomal aberrations. Some of those aberrations (dicentrics and micronuclei) are applied in biodosimetry. Biological dosimetry assumes similar radiosensitivity of each donor, but it does not exclude inter-individual variations in radiation susceptibility. Therefore, for biological reasons, it is always challenging to investigate inter-individual variability in response to radiation. For mechanistic reasons, it is also interesting to investigate the correlation between dicentric and micronuclei formation in response to radiation. In this experiment, irradiated blood specimens from 14 healthy male and female donors have been used to evaluate inter-individual variability in response to the genotoxic effects of X-ray radiation, as well as the dose-response relationship and test sensitivity using two endpoints (dicentrics and micronuclei). The results showed similar patterns of cytogenetic biomarker distribution between donors, but differences in the response of some donors at some doses. Data also showed that responses of male donors were better detected using the dicentric test, while for females, micronucleus frequencies were higher in response to the same dose of radiation. No influence of smoking status or age on specific responses was observed. Group variability in response to radiation was evaluated using coefficient of variation for each group of individuals irradiated with the same doses; as the dose increases, group variability becomes substantially lower. Despite sporadic inter-individual variability, trend of radiation-induced changes was similar. Produced calibration curves for both types of damage revealed dicentrics as genetic damage more typical for radiation than micronuclei.

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