Time filter

Source Type

Sofronic-Milosavljevic L.,University of Belgrade | Djordjevic M.,Serbian Institute of Meat Hygiene and Technology | Plavsic B.,Ministry of Agriculture | Grgic B.,Institute of Public Health of Serbia Milan Jovanovic Batut
Veterinary Parasitology | Year: 2013

In Serbia, infection with Trichinella spp. has been recognized as a human health and animal husbandry problem for almost a century. The rate of swine infection gradually decreased from 0.14% to 0.02% between 2001 and 2010. For the past 5 years, Trichinella infections among swine were detected at levels higher than 0.05% in 3 districts of Serbia while prevalence persisted at lower levels for the rest of the country. During this 10-year period, there were 2257 cases of human trichinellosis, including 3 deaths; however, a significant decrease in the number of cases was reported during the last 5 years (fewer than 200 cases per year). The fact that prevalence data presented here are similar to prevalence data from 1990 indicates that this period of 10 years was needed to overcome the re-emergence of Trichinella infection in swine and humans that occurred during the last decade of the previous century. © 2013 Elsevier B.V.


Ljubojevic D.,Scientific Veterinary Institute Novi Sad | Radosavljevic V.,Institute of Veterinary Medicine of Serbia | Puvaca N.,University of Novi Sad | Zivkov Balos M.,Scientific Veterinary Institute Novi Sad | And 3 more authors.
Journal of Food Composition and Analysis | Year: 2015

Fish meal and fish oil represent main protein and lipid sources in fish feed, but there is a growing need for sustainable alternatives to these ingredients. In this study, four isolipid extruded diets for carp (. Cyprinus carpio L.) were used: ROHP (rapeseed oil-high protein), FOHP (fish oil-high protein); ROLP (rapeseed oil-low protein) and FOLP (fish oil-low protein). The trial lasted 75 days. No statistically significant effect of oil source or protein level or interactions of these factors was observed in final body weight, growth parameters or in feed conversion ratio. Protein sparing effect and omega-3 sparing effect were observed in the present study. The results of this experiment showed no negative effects on growth parameters, or major detrimental effects on fatty acid composition of muscle tissue in common carp when fish fed with diets supplemented with rapeseed oil (RO). Furthermore, an enhanced protein sparing effect was observed, when fish fed with lower protein diets, but significant accumulation of crude lipid was observed as a result of lowering protein level. The results of this study showed that diets supplemented with rapeseed oil can be used successfully in common carp cage production. © 2014 Elsevier Inc.


Trailovic J.N.,University of Belgrade | Stefanovic S.,Serbian Institute of Meat Hygiene and Technology | Trailovic S.M.,University of Belgrade
British Poultry Science | Year: 2013

1. The objective of this study was to investigate in vitro and in vivo (in broiler chickens) ochratoxin A (OTA) adsorption efficiency of three different adsorbents: inorganic (modified zeolite); organic (esterified glucomannans) and mixed (inorganic and organic components plus enzymes). 2. The aim of the study was to investigate which of these adsorbents provided the best protection against the presence of residues of OTA in the pectoral muscle and liver of broilers given an OTA-contaminated diet. In addition, it was important to test and compare the results of adsorbent efficiency using two different in vitro methods. 3. The results from classical in vitro investigations carried out in the artificial intestinal fluid, showed that the inorganic adsorbent (Mz), exhibited the highest adsorption, having adsorbed 80.86 ± 1.85% of OTA, whereas average in vitro adsorption abilities of organic (30.52 ± 3.50%) and mixed (32.00 ± 2.60%) adsorbents were significantly lower. 4. In the investigation of absorption in everted sacs of broiler duodenal segments (Everted Duodenal Sacs Procedure), higher OTA adsorption in gut was exhibited by organic adsorbent, 74.26 ± 4.48%. Furthermore, the mean adsorption efficiency of mixed and inorganic adsorbent was 65.26 ± 4.76% and 45.75 ± 7.14%, respectively. 5. In the in vivo investigation, broilers were fed for 21 d on diets containing 2 mg/kg of OTA and supplemented with inorganic (Mz), organic (Ms) or mixed adsorbent (Mf) at the recommended concentration of 2 g/kg of feed. All three adsorbents significantly decreased OTA residue concentrations in the pectoral muscle and livers, but the order of effectiveness was mixed > organic > inorganic. The most efficient was the mixed adsorbent which decreased residue concentration by 72.50% in pectoral muscle and 94.47% in livers. 6. The Everted Duodenal Sac in vitro method provided results similar to those obtained in the in vivo study. However, further studies are required to investigate the efficiencies of adsorbents against various mycotoxins using this method. © 2013 Copyright British Poultry Science Ltd.


Kurcubic V.S.,University of Kragujevac | Maskovic P.Z.,University of Kragujevac | Vujic J.M.,University of Kragujevac | Vranic D.V.,Serbian Institute of Meat Hygiene and Technology | And 3 more authors.
Meat Science | Year: 2014

Fermented dry sausages (FDS) without nitrite added, fortified with bioactive phenol and flavonoid compounds originating from the ethanol extract of Kitaibelia vitifolia were food matrix for investigation of its antioxidant and antimicrobial potency. These activities were researched in order to improve the sausages' shelf-life, safety, and provide health benefits to consumers as well. The oxidative stability of the FDS, containing two different levels of natural preservative, was evaluated using five different contemporary methods for antioxidative activity. The activity was tested on the 20th day of the refrigerated storage. Minimum inhibitory concentrations of the sausage extract were determined against six microorganisms, using a micro dilution method. Determined optimal effective concentration of dissolved K. vitifolia extract (12.5. g/kg of meat dough) revealed strong antioxidant activity, and moderate antimicrobial activity against Escherichia coli (minimum inhibitory concentrations = 15.625. μg/mL). The modified sausages had typical chemical-physical characteristics of FDS, controlled on 0, 13, 26. d of ripening and 20, 40 and 60. d of storage. © 2014 Elsevier Ltd.


Antic D.,University of Novi Sad | Blagojevic B.,University of Novi Sad | Ducic M.,University of Novi Sad | Mitrovic R.,Serbian Institute of Meat Hygiene and Technology | And 2 more authors.
Meat Science | Year: 2010

A solution of natural, food-grade resin (Shellac) in ethanol was evaluated to treat samples of visually clean and dry cattle hides with the aim to reduce bacterial removability from the hides by swabbing. Hide treatment by 23% Shellac-in-ethanol solution reduced sponge-swabbing recoveries of general microflora (TVC) by a factor of 6.6 logs (>1000-fold larger than the 2.9 log reduction observed by ethanol alone), and of generic Escherichia coli and Enterobacteriaceae by factors of at least 2.9 and 4.8 logs, respectively. These reductions were superior to those achieved by a sanitizer rinse-vacuum hide treatment. Significantly greater reductions of TVC recoveries from hides were achieved when using higher Shellac concentrations (23 and 30% rather than 4.8-16.7%) and when Shellac solution temperatures were 20-40 °C rather than 50-60 °C. Furthermore, the Shellac-based treatment also markedly reduced the E. coli O157 prevalence (3.7-fold reduction) on natural, uninoculated hides, as well as the counts of E. coli O157 on artificially inoculated hides (2.1 log reduction). This preliminary study indicated that a "bacterial on-hide immobilisation" approach to reducing transmission of microorganisms from cattle hide is promising and so will be further explored. © 2009 Elsevier Ltd. All rights reserved.


Antic D.,University of Novi Sad | Blagojevic B.,University of Novi Sad | Ducic M.,University of Novi Sad | Nastasijevic I.,Serbian Institute of Meat Hygiene and Technology | And 2 more authors.
Food Control | Year: 2010

In the study, total numbers and distribution of hide microflora, as well as its transferability to meat via direct contact, were investigated. Average total viable counts of bacteria (TVC) and Enterobacteriaceae counts (EC) on 40 bovine hides (cattle from three geographic regions; slaughtered at single abattoir) were 6.7 and 4.3 log cfu/cm2, respectively. All hides contained generic Escherichia coli (GEC) but Salmonella spp. was not isolated from any bovine hide examined. Distal leg (metacarpus) and brisket areas were contaminated significantly heavier, in terms of TVC (6.9 and 7.1 log cfu/cm2, respectively), EC (4.5 and 4.3 log cfu/cm2, respectively) and GEC (occurrence 75% and 65%, respectively), than rump, flank and neck areas. No statistically significant differences in TVC, EC and GEC between the top layer and the lower layer (closer to skin) of the hide hair on visually clean hides were found. Only a small proportion (between 0.5 and 0.00002%) of total microflora (TVC) of hide was transmitted onto meat via direct contact under applied contact conditions (0.5-2 kg for 2 s; stationary or "sliding" contact). Nevertheless, the associated meat safety risks are assessed as significant, due to both high microbial loads existing on hide and high frequency of the hide-origin carcass cross contamination occurring in practice. © 2009 Elsevier Ltd. All rights reserved.


Blagojev N.,University of Novi Sad | Skrinjar M.,University of Novi Sad | Veskovic-Moracanin S.,Serbian Institute of Meat Hygiene and Technology | Soso V.,University of Novi Sad
Romanian Biotechnological Letters | Year: 2012

A wide spectrum of filamentous fungi is often found in various food commodities, where they can cause extensive damage and lead to sizable economic losses. The occurrence of their toxic metabolites - mycotoxins - constitutes a high risk for human and animal health. Although prevention of fungal growth and mycotoxin production on plants and in feedstuffs is usually considered as the best approach to impede the harmful effects on animal and human health, decontamination/detoxification of contaminated products is also of prime importance. Since the general public requires high quality, preservative free, safe but mildly processed food with extended shelf life, biopreservation, the control of one organism by another, has received much attention lately. Among the different potential decontaminating microorganisms, the group of the lactic acid bacteria has been considered as the most promising natural biological antagonists. Data have shown that many lactic acid bacteria can inhibit mould growth and that some of them have the potential to interact with mycotoxins. This review summarizes recent data about potential control of mould growth and mycotoxin production by lactic acid bacteria and highlights that they are very promising biological agents for food safety. © 2012 University of Bucharest.


Milicevic D.R.,Serbian Institute of Meat Hygiene and Technology | Skrinjar M.,University of Novi Sad | Baltic T.,Serbian Institute of Meat Hygiene and Technology
Toxins | Year: 2010

Mycotoxins are toxic compounds, produced by the secondary metabolism of toxigenic moulds in the Aspergillus, Alternar ia, Claviceps, Fusarium, Penicilliu m and Stachybotrys genera occurring in food and feed commodities both pre- and post-harvest. Adverse human health effects from the consumption of mycotoxins have occurred for many centuries. When ingested, mycotoxins may cause a mycotoxicosis which can result in an acute or chronic disease episode. Chronic conditions have a much greater impact, numerically, on human health in general, and induce diverse and powerful toxic effects in test systems: some are carcinogenic, mutagenic, teratogenic, estrogenic, hemorrhagic, immunotoxic, nephrotoxic, hepatotoxic, dermotoxic and neurotoxic. Although mycotoxin contamination of agricultural products still occurs in the developed world, the application of modern agricultural practices and the presence of a legislatively regulated food processing and marketing system have greatly reduced mycotoxin exposure in these populations. However, in developing countries, where climatic and crop storage conditions are frequently conducive to fungal growth and mycotoxin production, much of the population relies on subsistence farming or on unregulated local markets. Therefore both producers and governmental control authorities are directing their efforts toward the implementation of a correct and reliable evaluation of the real status of contamination of a lot of food commodity and, consequently, of the impact of mycotoxins on human and animal health. © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.


Milicevic D.R.,Serbian Institute of Meat Hygiene and Technology | Stefanovic S.,Serbian Institute of Meat Hygiene and Technology | Jankovic S.,Serbian Institute of Meat Hygiene and Technology | Radicevic T.,Serbian Institute of Meat Hygiene and Technology
Veterinary World | Year: 2012

Aim: The aim of this study was to check the current status of OTA contamination of the Serbian pork and chicken meat in naturally exposed animals which are part of chain production. The knowledge of these factors, their importance and significance, can be used as a tool from the food safety point of view to predict if OTA content in pork and chicken tissues poses a risk to human health and deleterious effects on animals' health and well-being. Materials and Methods: A total of 540 samples of pigs blood, kidney, liver and chicken gizzard were randomly selected from slaughtered pigs (n=90) and chicken (n=90) and analyzed for the presence of ochratoxin A (OTA) by High-performance liquid chromatography (HPLC-FL). Results: In porcine tissue samples, of the 90 liver samples, 26.6% contained OTA in the range of 0.22-14.5 ng/g. The incidence of OTA in serum and kidneys were very similar (31% and 33.3%, respectively), with a maximum concentration of 220.8 ng/mL, and 52.5 ng/g, respectively. Majority of chickens' tissues samples, were not found to contain measurable amounts of OTA. Moreover, the OTA levels found in analysed tissues were low in general. Conclusion: The results of this preliminary research indicate that the content of OTA in the examined tissues are far below the values that represent hazard to the health of consumers. However, the results of this study show that in Serbia, consumers are nevertheless frequently exposed to ochratoxins.


Spiric A.,Serbian Institute of Meat Hygiene and Technology | Trbovic D.,Serbian Institute of Meat Hygiene and Technology | Vranic D.,Serbian Institute of Meat Hygiene and Technology | Djinovic J.,Serbian Institute of Meat Hygiene and Technology | And 2 more authors.
Analytica Chimica Acta | Year: 2010

Studies performed on lipid extraction from animal and fish tissues do not provide information on its influence on fatty acid composition of the extracted lipids as well as on cholesterol content. Data presented in this paper indicate the impact of extraction procedures on fatty acid profile of fish lipids extracted by the modified Soxhlet and ASE (accelerated solvent extraction) procedure. Cholesterol was also determined by direct saponification method, too. Student's paired t-test used for comparison of the total fat content in carp fish population obtained by two extraction methods shows that differences between values of the total fat content determined by ASE and modified Soxhlet method are not statistically significant. Values obtained by three different methods (direct saponification, ASE and modified Soxhlet method), used for determination of cholesterol content in carp, were compared by one-way analysis of variance (ANOVA). The obtained results show that modified Soxhlet method gives results which differ significantly from the results obtained by direct saponification and ASE method. However the results obtained by direct saponification and ASE method do not differ significantly from each other. The highest quantities for cholesterol (37.65 to 65.44. mg/100. g) in the analyzed fish muscle were obtained by applying direct saponification method, as less destructive one, followed by ASE (34.16 to 52.60. mg/100. g) and modified Soxhlet extraction method (10.73 to 30.83. mg/100. g). Modified Soxhlet method for extraction of fish lipids gives higher values for n-6 fatty acids than ASE method (tpaired=3.22 tc=2.36), while there is no statistically significant difference in the n-3 content levels between the methods (tpaired=1.31). The UNSFA/SFA ratio obtained by using modified Soxhlet method is also higher than the ratio obtained using ASE method (tpaired=4.88 tc=2.36). Results of Principal Component Analysis (PCA) showed that the highest positive impact to the second principal component (PC2) is recorded by C18:3 n-3, and C20:3 n-6, being present in a higher amount in the samples treated by the modified Soxhlet extraction, while C22:5 n-3, C20:3 n-3, C22:1 and C20:4, C16 and C18 negatively influence the score values of the PC2, showing significantly increased level in the samples treated by ASE method. Hotelling's paired T-square test used on the first three principal components for confirmation of differences in individual fatty acid content obtained by ASE and Soxhlet method in carp muscle showed statistically significant difference between these two data sets (T2=161.308, p<0.001). © 2010 Elsevier B.V.

Loading Serbian Institute of Meat Hygiene and Technology collaborators
Loading Serbian Institute of Meat Hygiene and Technology collaborators