Smederevska Palanka, Serbia
Smederevska Palanka, Serbia

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Dordevic R.,Serbian Institute for Vegetable Crops | Zecevic B.,Serbian Institute for Vegetable Crops | Zdravkovic J.,Serbian Institute for Vegetable Crops | Todorovic G.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic
Genetika | Year: 2010

The aim of the present study was to estimate, on the basis of diallel crossing, superior-parent heterosis, components of genetic variability and trait heritability for three yield components in tomato, and to perform the VrWr regression analysis. Six different tomato inbred lines originating from local and introduced breeding material were selected for the study. The hybrids expressed greater mean values for the majority of the traits than the inbred lines. The value of additive component of variance (D) was higher than the value of the dominant variance (H 1 and H 2) for the number of fruits per plant and the average fruit weight, while the value of the dominant component of variance was higher for the fruit weight per plant. Positive values of additive x dominant genetic effect interaction (F) for the observed traits point to a greater participation of dominant alleles in the inheritance of these traits, which was confirmed by the coefficients H 2/4H 1 (0.208-0.228) and by the ratio KD/KR being greater than unity (1.129-1.536). The values of the average degree of dominance √H 1/D, lower than unity for the number of fruits per plant and the average fruit weight, indicate that these traits were inherited by partial dominance. Furthermore, values of the degree of dominance greater than unity for the fruit weight indicate that this trait was inherited by dominance or superdominance. These conclusions were also confirmed by high values of the broad- and narrow-sense heritability that varied from 98.88% to 99.44%, i.e., from 45.06 to 87.51%, respectively, as well as, by the VrWr regression for the observed traits in the F 1 generation.


Zdravkovic J.,Serbian Institute for Vegetable Crops | Pavlovic N.,Serbian Institute for Vegetable Crops | Girek Z.,Serbian Institute for Vegetable Crops | Brdar-Jokanovic M.,Serbian Institute for Vegetable Crops | And 2 more authors.
Pakistan Journal of Botany | Year: 2011

Tomato yield depends on two components viz., fruit weight and number of fruit per flower branch. These traits are quantitative andtherefore influenced by multiple genes. The objective of this study was to estimate the main gene effects (additive, dominant and digenicepistasis) and to determine the mode of inheritance for yield components and yield in three tomato cross combinations by generation meananalysis. The trial included genotypes differing in morphology and yield: line DAT, cultivar SP-109 and local population KGZ. The analyzedgenotypes belong to tomato germplasm collection of the Institute for Vegetable Crops, Smederevska Palanka, Serbia. Six families per hybrid,including parents, have been tested. Besides, the additive and dominance gene effects, non-allelic gene interactions have been detected for yieldcomponents and yield. Duplicate type of epistasis was confirmed for fruit weight and yield in all cases characterized by significant dominanceand dominance/dominance effects, which diminishes the effect of dominant genes and makes breeding for yield increase more difficult.Therefore, number of fruits per flower branch increase would be the most efficient strategy for increasing tomato yielding ability.


Cosic M.,University of Belgrade | Djurovic N.,University of Belgrade | Todorovic M.,Mediterranean Agronomic Institute of Bari | Maletic R.,University of Belgrade | And 2 more authors.
Agricultural Water Management | Year: 2015

The present paper reports research that focused on the effect of kaolin on the yield, quality and water use efficiency of the sweet pepper Capsicum annuum L., grown under different irrigation regimes. The research was conducted in an open field with carbonate chernozem soil, at Stara Pazova (40. km north of Belgrade, Serbia). It lasted for three years (2011, 2012, and 2013). Three irrigation regimes and two kaolin treatments were compared. The irrigation regimes were: (i) full irrigation (F) ensuring 100% of crop evapotranspiration (ETc), (ii) deficit irrigation at 80% ETc (R1), and (iii) deficit irrigation at 70% ETc (R2). The kaolin treatments were: (i) control without kaolin (C) and (ii) treatment with kaolin application (K). The setup was a two-factorial, completely random block system, with three replications. The first factor was the irrigation regime and the second kaolin application.On average, the highest fresh sweet pepper yields were achieved under full irrigation (10kgm-2). Also, FC and FK treatments resulted in the highest first-class, second-class and first+second class yields. On average, the lowest percentage of sunburn was noted in the case of the FK treatment (10%), and the highest with the R2C treatment (about 27%). The sugar content of the pepper was quite consistent, while the organic acid content varied from 15.0mLg-1 with R1C to 18.7mLg-1 with FK. The application of kaolin and the irrigation regime did not have a statistically significant effect on the antioxidant activity of the pepper and ranged from 5538.4 to 6447.4μmol TUg-1. The highest yield water use efficiency (yWUE) of first-class and first+second class yields was recorded with the FC, FK and R1C treatments. Throughout the study period, yWUE levels of the second-class yields, and of the total yield, were rather uniform, regardless of the type of treatment. © 2015 Elsevier B.V.


Djordjevic M.,Serbian Institute for Vegetable Crops | Djordjevic O.,University of Belgrade | Djordjevic R.,Serbian Institute for Vegetable Crops | Mijatovic M.,Serbian Institute for Vegetable Crops | And 3 more authors.
Pakistan Journal of Botany | Year: 2013

Essential oils of Mentha piperita, Eucaliptus globulus, Pinus sylvestris, Rosmarinus officinalis, Pimpinella anisum and Origanum vulgare were studied for antifungal effect of their volatile phase toward tomato pathogen (Fusarium oxysporum f.sp. lycopersici) In vitro. Antifungal effect of oils was expressed through calculating percentage of inhibition of radial growth of mycelia of pathogen, and by determining MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration). Results indicated that all of examined oils expressed antifungal activity in different concentrations. Essential oil of oregano (Origanum vulgare) was the most efficient in inhibition of mycelial growth with total inhibition applied at lowest concentration of 0.04 μl/ml of air. MIC and MFC for this oil were also found at 0.04 μl/ml of air. Oils of anise (Pimpinella anisum) and menthe (Mentha piperita) were also very effective. Anise oil had totally inhibited mycelium applied at 0.1 μl/ml of air. MIC value was 0.3 μl/ml of air, while MFC was higher than maximal applied concentration (>0.6 μl/ml of air). Menthe oil had total inhibition of mycelial growth applied at 0.3 μl/ml of air and the MIC value was at the same point. MFC was higher than maximal applied concentration (>0.6 μl/ml of air). Other essential oils expressed high inhibition rate but it wasn't 100%. The results of this research indicate that essential oil of oregano followed by oils of menthe and anise has potential to suppress the mycelial growth Fusarium oxysporum f. sp. lycopersici, In vitro. Further research should provide answers for final goal - applying essential oils In vivo.


Pavlovic S.,Serbian Institute for Vegetable Crops | Vinterhalter B.,University of Belgrade | Zdravkovic-Korac S.,University of Belgrade | Vinterhalter D.,University of Belgrade | And 3 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2013

A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower. © 2012 Springer Science+Business Media Dordrecht.


Zdravkovic J.,Serbian Institute for Vegetable Crops | Pavlovic N.,Serbian Institute for Vegetable Crops | Girek Z.,Serbian Institute for Vegetable Crops | Zdravkovic M.,Serbian Institute for Vegetable Crops | Cvikic D.,Serbian Institute for Vegetable Crops
Genetika | Year: 2010

The remarkable development and application of new genetic The Institute for Vegetable Crops possesses a rich germplasm collection of vegetables, utilized as gene resource for breeding specific traits. Onion and garlic breeding programs are based on chemical composition improvement. There are programs for identification and use of genotypes characterized by high tolerance to economically important diseases. Special attention is paid to breeding cucumber and tomato lines tolerant to late blight. As a result, late blight tolerant pickling cucumber line, as well as late blight tolerant tomato lines and hybrids are realized. Research on bean drought stress tolerance is initiated. Lettuce breeding program including research on spontaneous flora is started and interspecies hybrids were observed as possible genetic variability source. It is important to have access to a broad range of vegetable genotypes in order to meet the needs of organic agriculture production. Appreciating the concept of sustainable agriculture, it is important to introduce organic agriculture programs in breeding institutions.


Milosevic M.,Ministry of Agriculture | Vujakovic M.,Serbian Institute for Vegetable Crops | Karagic D.,Serbian Institute for Vegetable Crops
Genetika | Year: 2010

Seed marks the beginning of each plant production and therefore ensuring its quality is the priority of modern seed science and a prerequisite for obtaining high yields of all plant species. Determination of seed quality and its viability indicates what seed lots can be placed onto the market, and for that reason it is very important to have reliable methods and tests to be used for seed quality and seed vigour testing. The term vigour of viability is used to describe the physiological characteristics of seeds that control its ability to germinate rapidly in the soil and to tolerate various, mostly negative environmental factors. MCDONALD grouped vigour tests into three groups: Physical tests - determine seed characteristics such as size and mass. These tests are inexpensive, quick, can be applied to large number of samples, and are positively correlated with seed vigour. The main feature of seed development is accumulation of nutritive materials, which is also in direct correlation with vigour, i.e. with size and mass of seed; Physiological tests - using germination and growth parameters. There are two types of these tests. First type, when germination is done under favourable conditions (standard laboratory germination, and test of growth intensity). Second type, when seed is exposed to unfavourable environmental conditions (cold test, accelerated aging test, and Hiltner test); Biochemical tests - are considered as indirect methods for estimation of seed value. These are Tetrazolijum test, conductometric measurements, enzyme activity and respiration.


Brdar-Jokanovic M.,Serbian Institute of Field and Vegetable Crops | Girek Z.,Serbian Institute for Vegetable Crops | Pavlovic S.,Serbian Institute for Vegetable Crops | Ugrinovic M.,Serbian Institute for Vegetable Crops | Zdravkovic J.,Serbian Institute for Vegetable Crops
Genetika | Year: 2014

This research was conducted with the aim to among forty-one tested tomato (Lycopersicon esculentum Mill) populations distinguish those tolerant to limited water supply. Tolerance assessments were performed by using sixteen drought stress selection indices calculated on the basis of tomato shoot and root dry weight yields determined at water stress and non-stress conditions. Populations were differentiated in groups using the method of cluster analysis. The pot experiment was set in controlled greenhouse conditions and comprised optimally irrigated control and drought treatment (35.0 and 20.9% volumetric soil water content, respectively), imposed at the phase of intensive vegetative growth. The experiment was conducted at the Institute for Vegetable Crops in Smederevska Palanka, Serbia. The analyzed tomatoes exhibited significant differences in terms of response to limited irrigation, which had more pronounced effect on shoot dry weight than on the roots (average decrease of 64.4 and 35.7%, respectively). Consequently, root fraction in the total dry weight increased at drought for 68.2% on average. Shoot and root dry weights were positively correlated at optimal irrigation but not in drought, implying genotypic differences in terms of root adjustments to stress conditions. As for the calculated selection indices, substantial variation was found among the populations enabling their ranking in terms of drought tolerance. Since ranking was not the same in all cases, clustering the populations was performed taking into account all sixteen selection indices. The results of this analysis indicate that populations designated with numbers 126, 124, 131, 125, 128, 105, 101, 138, 110, 132 and 109 in Institute for Vegetable Crops germplasm collection exhibit satisfactory level of drought tolerance at vegetative phase and therefore may be used as parents in breeding programs.


Zdravkovic J.M.,Serbian Institute for Vegetable Crops | Acamovic-Djokovic G.S.,University of Kragujevac | Mladenovic J.D.,University of Kragujevac | Pavlovic R.M.,University of Kragujevac | Zdravkovic M.S.,Serbian Institute for Vegetable Crops
Hemijska Industrija | Year: 2014

The antioxidant activity of three lettuce varieties (Lactuca sativa L.) Emerald, Vera and Neva, cultivated in two kinds of protected spaces, a glasshouse and a plastic greenhouse, under controlled conditions, was determined. The content of antioxidant compounds: total phenols, flavonoids, L-ascorbic acid, β-carotene and lycopene, were determined in etha- nolic extracts of the lettuce with spectrophotometric methods. The largest content of total phenols (78.98±0.67 mg GAE/g of dry extract) was found in ethanolic extract of the lettuce variety Neva cultivated in a plastic greenhouse, whereas the largest content of flavonoids (35.45±0.95 mg RU/g of dry extract) was displayed in the lettuce Emerald cultivated in a glasshouse. It was observed that the lettuce cultivated in the glasshouse contained a somewhat higher content of L-ascorbic acid than the lettuce same variety from plastic greenhouse. The content of lycopene in the examined lettuce is negligible, and the content of β-carotene is low. On the other hand, the high content of phenolic components causes favourable antioxidant properties found in all varieties of examined lettuce.


Mihailovic V.,Serbian Institute for Vegetable Crops | Mikic A.,Serbian Institute for Vegetable Crops | Cupina B.,Serbian Institute for Vegetable Crops | Krstic D.,Serbian Institute for Vegetable Crops | And 2 more authors.
Legume Research | Year: 2013

Grass pea (Lathyrus sativus L.) is a grain and forage legume crop. A small plot trial was carried out from 2004to 2006, comprising ten grass pea accessions from the Annual Forage Legumes Collection of the Institute of Held and Vegetable Crops in Novi Sad, Serbia. PL 114 615 had the highest green forage yield (50.7 t ha-1), while Le Cambou had the highest hay yield (9.0t ha-1). Green forage yield (t ha-1) was highly correlated with number of intemodes (r = 0.947 and r = 0.820), while hay yield (t ha-1) was highly correlated with green forage yields (r = 0.835 and r = 0.898). Grass pea has considerable potential to produce high yields of green forage and hay under conditions in Serbia, especially its northern province of Vojvodina with its rich chernozem soils.

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