Serbian Institute for Medicinal Plant Research Dr Josif Pancic

Belgrade, Serbia

Serbian Institute for Medicinal Plant Research Dr Josif Pancic

Belgrade, Serbia
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Stojkovic D.S.,University of Belgrade | Zivkovic J.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Sokovic M.,University of Belgrade | Glamoclija J.,University of Belgrade | And 3 more authors.
Food and Chemical Toxicology | Year: 2013

This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L. monocytogenes development in cream cheese, at room temperature and in refrigerator (25. °C and 4. °C, respectively), after 3. days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. © 2013 Elsevier Ltd.


Zivkovic J.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Cebovic T.,University of Novi Sad | Maksimovic Z.,University of Belgrade
Central European Journal of Biology | Year: 2012

The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe 2+/g, and DPPH IC 50 values from 12.58 to 66.34 μg/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe 2+/g; DPPH IC 50: 9.57 μg/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl 4). Pretreatment with 100 mg/kg b. w. of Veronica extracts inhibited CCl 4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl 4-induced liver damage by their antioxidative effect on hepatocytes. © 2012 Versita Warsaw and Springer-Verlag Wien.


Ivanovic J.,University of Belgrade | Dimitrijevic-Brankovic S.,University of Belgrade | Misic D.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Zizovic I.,University of Belgrade
Journal of Functional Foods | Year: 2013

For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56mgGAE/gextract). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC≤1.25μg/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products. © 2012 Elsevier Ltd.


Ivanovic J.,University of Belgrade | Misic D.,University of Belgrade | Zizovic I.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic
Food Control | Year: 2012

Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 μg/ml, followed by rosemary (MIC = 320-1280 μg/ml) and sage (MIC = 160-2560 μg/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method. © 2011 Elsevier Ltd.


Popovic Z.,University of Belgrade | Smiljanic M.,University of Belgrade | Kostic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Nikic P.,University of Belgrade | Jankovic S.,Serbian Institute for Science Application in Agriculture
Indian Journal of Traditional Knowledge | Year: 2014

This study reports data on traditional phytotherapy regarding wild flora in the Deliblato Sands, the Special Nature Reserve located in Serbia, South East Europe. Though interviewing local people on identification of plants, plant parts used, methods of remedy preparation and administration, 231 wild plant species of medicinal importance from 172 genera and 62 families, and 101 original preparations were documented. A high degree of consensus (ICF > 0.45) was observed among informants when treating the most common ailments: wound infections, insect/snake bites, blood purification, menstrual and nervous disorders, bronchial/lung catarrhs, cough, cardiac disorders, constipation, diarrhea, urinary infections, rheuma. Most of medicinal plants listed by informants were available, while seven of them were classified as endangered and entered into the system of statutory protection. This study confirmed the extensive indigenous knowledge on medicinal plants, and pointed to some ways of usage that have not been documented in the literature.


Glisic S.B.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Skala D.U.,University of Belgrade
Ultrasonics Sonochemistry | Year: 2011

A wide spectrum of phytochemicals could be isolated from sage (Salvia officinalis L.) using different extraction or distillation technique: the supercritical fluid extraction (SFE), the volatiles compounds (monoterpenes and sesquiterpenes) isolation using hydrodistillation or higher molecular compounds with Soxhlet extraction or ultrasound-assisted extraction. The combination of ultrasound-assisted extraction followed by re-extraction of obtained extract with supercritical CO2 was performed in this study. The goal of performed investigation was to concentrate diterpenes present in sage extract which are generally considered to be responsible for antioxidant activity of extracted compounds. The fractionation using the supercritical CO2, and different combination of the ultrasound-assisted solvent extractions (water-ethanol mixture or only water) followed by supercritical CO2 re-extraction of obtained extract or treated plant material were analyzed and compared. Based on the results of these investigations it could be proposed the best extraction procedure: the ultrasound pretreatment of plant material with distilled water and re-extraction of plant material (residue) using supercritical CO2. That procedure gives two valuable products: the ultrasound extract which is rich in sugars and possess the immunomodulatory activity and supercritical extract which is rich in diterpenes and sesquiterpenes. © 2010 Elsevier B.V.


Ivanovic J.,University of Belgrade | Zizovic I.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Stamenic M.,University of Belgrade | Skala D.,University of Belgrade
Journal of Supercritical Fluids | Year: 2011

The goal of present work was to investigate and explain kinetics and mass transfer phenomena occurring during the SFE from the mixture of two plants with different initial composition. The extractions from pure clove, oregano and thyme as well as from clove/oregano (C/O) and clove/thyme (C/T) mixtures with various initial compositions of plant material were carried out using supercritical CO2 at 10 MPa and 40 °C. The results indicated that presence of light compounds in supercritical CO2 originated from the oregano leaves or thyme at the beginning of extraction process increases the extraction rate of compounds from clove bud. Only small added amounts of oregano or thyme to clove bud (C/O - 90:10%, w/w; or C/T - 84:16%, w/w) in the starting plant mixture had the same effect resulted in the similar and the highest increase of the extraction rate and had negligible influence on total extraction yield compared to extract isolated from pure clove. Different mathematical models were used for simulation of experimental data which showed that the highest increase of the solubility of extractable compounds in supercritical CO2 as well as the highest mass transfer rate in the solid phase during extractions existed during extraction from C/O (90:10, w/w) and C/T (84:16, w/w) mixtures. Decrease of SC CO2 consumption or shorter time of extraction necessary for achieving desired extract yield in the case of SFE of the clove buds could be important for industrial-scale application. © 2010 Elsevier B.V. All rights reserved.


Ivanovic J.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Skala D.,University of Belgrade
Journal of Supercritical Fluids | Year: 2011

Kinetics and selectivity of supercritical carbon dioxide (SC CO 2) extraction of Helichrysum italicum flowers were analyzed at pressures in the range of 10-20 MPa and temperatures of 40 °C and 60 °C (density of SC CO2 from 290 to 841 kg/m3) and also at 10 MPa and 40 °C using flowers with different moisture contents (10.5% and 28.4%). Increased moisture content of H. italicum flowers resulted in enchased solubility of solute enabling decrease of SC CO2 consumption necessary for achieving desired extraction yield. The most abundant compounds in the supercritical extracts are sesquiterpenes and waxes while monoterpenes and sesquiterpenes are the main constituents of essential oil obtained by hydrodistillation. The optimal set of working parameters with respect to extraction yield, SC CO2 consumption and chemical composition of extract were defined related to moisture content of raw material and SC CO 2 density. © 2011 Elsevier B.V.


Stojkovic D.,University of Belgrade | Sokovic M.,University of Belgrade | Glamoclija J.,University of Belgrade | Dzamic A.,University of Belgrade | And 3 more authors.
Food Chemistry | Year: 2011

The following study deals with the chemical composition, antimicrobial activity of essential oils of Vitex agnus-castus L. and their main constituents in vitro and in vivo. The main compounds in the oil of unripe fruits were sabinene (17.8%) and 1,8-cineole (17.5%), while in the oil of the ripe fruits dominant compounds were 1,8-cineole (16.3%) and sabinene (13.4%). The leaves oil contained an abundance of 1,8-cineole (22.0%), as well. All of the oils tested were rich sources of α-pinene (12.2%, 9.4% and 9.4%, respectively). Antimicrobial activity was tested using bacterial and fungal strains by the microdilution method. Using the same technique 1,8-cineole and α-pinene showed very high antimicrobial potency as well. As 1,8-cineole was the predominant constituent of the oils, we have chosen to test it further in an in vivo experiment. Randomly chosen apples were treated with 1,8-cineol solution and infected with Aspergillus niger in order to provoke Aspergillus rot in apples. Disease incidence was recorded. © 2011 Elsevier Ltd. All rights reserved.


Pavel M.,University of Bucharest | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Stevic T.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic
Journal of the Serbian Chemical Society | Year: 2010

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), γ-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and β-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 μl/ml. 2009 Copyright (CC) SCS.

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