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Zivkovic J.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Cebovic T.,University of Novi Sad | Maksimovic Z.,University of Belgrade
Central European Journal of Biology | Year: 2012

The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe 2+/g, and DPPH IC 50 values from 12.58 to 66.34 μg/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe 2+/g; DPPH IC 50: 9.57 μg/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl 4). Pretreatment with 100 mg/kg b. w. of Veronica extracts inhibited CCl 4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl 4-induced liver damage by their antioxidative effect on hepatocytes. © 2012 Versita Warsaw and Springer-Verlag Wien.

Ivanovic J.,University of Belgrade | Dimitrijevic-Brankovic S.,University of Belgrade | Misic D.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Zizovic I.,University of Belgrade
Journal of Functional Foods | Year: 2013

For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56mgGAE/gextract). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC≤1.25μg/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products. © 2012 Elsevier Ltd.

Ivanovic J.,University of Belgrade | Misic D.,University of Belgrade | Zizovic I.,University of Belgrade | Ristic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic
Food Control | Year: 2012

Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 μg/ml, followed by rosemary (MIC = 320-1280 μg/ml) and sage (MIC = 160-2560 μg/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method. © 2011 Elsevier Ltd.

Popovic Z.,University of Belgrade | Smiljanic M.,University of Belgrade | Kostic M.,Serbian Institute for Medicinal Plant Research Dr Josif Pancic | Nikic P.,University of Belgrade | Jankovic S.,Serbian Institute for Science Application in Agriculture
Indian Journal of Traditional Knowledge | Year: 2014

This study reports data on traditional phytotherapy regarding wild flora in the Deliblato Sands, the Special Nature Reserve located in Serbia, South East Europe. Though interviewing local people on identification of plants, plant parts used, methods of remedy preparation and administration, 231 wild plant species of medicinal importance from 172 genera and 62 families, and 101 original preparations were documented. A high degree of consensus (ICF > 0.45) was observed among informants when treating the most common ailments: wound infections, insect/snake bites, blood purification, menstrual and nervous disorders, bronchial/lung catarrhs, cough, cardiac disorders, constipation, diarrhea, urinary infections, rheuma. Most of medicinal plants listed by informants were available, while seven of them were classified as endangered and entered into the system of statutory protection. This study confirmed the extensive indigenous knowledge on medicinal plants, and pointed to some ways of usage that have not been documented in the literature.

Stojkovic D.,University of Belgrade | Sokovic M.,University of Belgrade | Glamoclija J.,University of Belgrade | Dzamic A.,University of Belgrade | And 3 more authors.
Food Chemistry | Year: 2011

The following study deals with the chemical composition, antimicrobial activity of essential oils of Vitex agnus-castus L. and their main constituents in vitro and in vivo. The main compounds in the oil of unripe fruits were sabinene (17.8%) and 1,8-cineole (17.5%), while in the oil of the ripe fruits dominant compounds were 1,8-cineole (16.3%) and sabinene (13.4%). The leaves oil contained an abundance of 1,8-cineole (22.0%), as well. All of the oils tested were rich sources of α-pinene (12.2%, 9.4% and 9.4%, respectively). Antimicrobial activity was tested using bacterial and fungal strains by the microdilution method. Using the same technique 1,8-cineole and α-pinene showed very high antimicrobial potency as well. As 1,8-cineole was the predominant constituent of the oils, we have chosen to test it further in an in vivo experiment. Randomly chosen apples were treated with 1,8-cineol solution and infected with Aspergillus niger in order to provoke Aspergillus rot in apples. Disease incidence was recorded. © 2011 Elsevier Ltd. All rights reserved.

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