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Mórahalom, Hungary

Tukacs-Hajos A.,GazInnov Ltd | Pap B.,Seqomics Biotechnology Ltd. | Maroti G.,Hungarian Academy of Sciences | Szendefy J.,Biogaz Fejleszto Ltd. | And 2 more authors.
Bioresource Technology | Year: 2014

Anaerobe fermentation of sugar beet pressed pulp was investigated in pilot-scale digesters. Thermophilic adaptation of mesophilic culture was monitored using chemical analysis and metagenomic characterization of the sludge. Temperature adaptation was achieved by increasing the temperature gradually (2°Cday-1) and by greatly decreasing the OLR. During stable run, the OLR was increased gradually to 11.29kgVSm-3d-1 and biogas yield was 5% higher in the thermophilic reactor. VFA levels increased in the thermophilic reactor with increased OLR (acetic acid 646mgL-1, propionic acid 596mgL-1), then VFA decreased and the operation was manageable beside the relative high tVFA (1300-2000mgL-1). The effect of thermophilic adaptation on the microbial communities was studied using a sequencing-based metagenomic approach. Connections between physico-chemical parameters and populations of bacteria and methanogen archaea were revealed. © 2014 Elsevier Ltd.

Svab D.,Hungarian Academy of Sciences | Balint B.,Seqomics Biotechnology Ltd. | Maroti G.,Hungarian Academy of Sciences | Toth I.,Hungarian Academy of Sciences
Infection, Genetics and Evolution | Year: 2015

Shiga toxin-producing Escherichia coli (STEC), and especially enterohaemorrhagic E. coli (EHEC) are important, highly virulent zoonotic and food-borne pathogens. The genes encoding their key virulence factors, the Shiga toxins, are distributed by converting bacteriophages, the Stx phages. In this study we isolated a new type of inducible Stx phage carrying the stx1 gene cluster from the prototypic EHEC O157:H7 Sakai strain. The phage showed Podoviridae morphology, and was capable of converting the E. coli K-12 MG1655 strain to Shiga toxin-producing phenotype. The majority of the phage genes originate from the stx2-encoding Sakai prophage Sp5, with major rearrangements in its genome. Beside certain minor recombinations, the genomic region originally containing the stx2 genes in Sp5 was replaced by a region containing six open reading frames from prophage Sp15 including stx1 genes. The rearranged genome, together with the carriage of stx1 genes, the morphology and the capability of lysogenic conversion represent a new type of recombinant Stx1 converting phage from the Sakai strain. © 2014 Elsevier B.V.

Toth I.,Hungarian Academy of Sciences | Svab D.,Hungarian Academy of Sciences | Balint B.,Seqomics Biotechnology Ltd. | Brown-Jaque M.,University of Barcelona | Maroti G.,Hungarian Academy of Sciences
Infection, Genetics and Evolution | Year: 2016

Here we report the first complete nucleotide sequence of a Shiga toxin (Stx) converting phage from a Shigella sonnei clinical isolate that harbors stx1 operon, first identified in the chromosome of Shigella dysenteriae type 1. The phage named Shigella phage 75/02 Stx displayed Podoviridae morphology. It proved to be transferable to Escherichia coli K-12 strains, and cytotoxicity of the lysogenized strains was demonstrated in Vero cell cultures. Genomic analysis revealed that the prophage genome is circular and its size is 60,875 nt that corresponds to 76 ORFs. The genome of Shigella phage 75/02 Stx shows a great degree of mosaic structure and its architecture is related to lambdoid phages. All the deduced proteins, including the 37 hypothetical proteins showed significant homologies to Stx phage proteins present in databases. The phage uniformly inserted into the ynfG oxidoreductase gene framed by phage integrase and antirepressor genes in parental S. sonnei and in the three lysogenized K-12 strains C600, DH5α and MG1655. The Stx1 prophage proved to be stable in its bacterial hosts and remained inducible. © 2015 Elsevier B.V..

Boboescu I.Z.,Polytechnic University of Timisoara | Gherman V.D.,Polytechnic University of Timisoara | Mirel I.,Polytechnic University of Timisoara | Pap B.,Seqomics Biotechnology Ltd. | And 8 more authors.
International Journal of Hydrogen Energy | Year: 2014

Biohydrogen production from synthetic wastewater as substrate was studied in anaerobic small scale batch reactors. Enriched anaerobic mixed consortia sampled from various environments were used as parent inocula to start the bioreactors. Selective enrichments were achieved by various physical and chemical pretreatments and changes in the microbial communities were monitored by metagenomic and molecular diagnostics approaches. Experimental data showed the feasibility of biohydrogen production using synthetic wastewater as substrate. The hydrogen generation capability of the different mixed consortia is clearly dependent on the pretreatment methods. The described approach opens the possibility for an alternative way towards simultaneous wastewater treatment and renewable energy generation. Copyright © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Nagymihaly M.,Hungarian Academy of Sciences | Szucs A.,Hungarian Academy of Sciences | Kereszt A.,Hungarian Academy of Sciences | Kereszt A.,Seqomics Biotechnology Ltd.
Acta Biologica Szegediensis | Year: 2015

Next-Generation Sequencing (NGS) originally refers to high-throughput, massively parallel sequencing methods that allow the sequencing of up to billions of small (50-1000 bp), amplified DNA fragments at the same time but nowadays, there are NGS techniques that determine the sequence of long (up to 50 kbp) single molecules. Over the past years, NGS technologies become widely available with increasing throughput and decreasing sequencing costs per base making them more cost effective than the previously used capillary sequencing methods based on Sanger biochemistry. Nowadays, high-throughput DNA sequencing is routinely used on a wide range of important fields of biology and medicine enabling large-scale sequencing projects like analysis of complete genomes, disease association studies, whole transcriptomes, methylomes and provide new insights into complex biological systems. In addition, more and more NGS-based diagnostic tools are being introduced into the clinical practice, for example, on the fields of oncology, inherited and infectious diseases or pre-implantation and prenatal genetic screenings.

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