Separation Science Laboratory

Kempton, South Africa

Separation Science Laboratory

Kempton, South Africa
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Korsgren P.,Separation Science Laboratory | Weinmann A.L.,Astrazeneca
American Pharmaceutical Review | Year: 2012

Purification of crude compounds in drug discovery is often performed using RP HPLC with mixed (UV/MS) triggered fraction collection. This technique allows high-throughput achiral separations to be performed using traditional reversed-phase stationary phases, but the platform is not successful for all compounds. Recent developments in SFC-MS have allowed implementation of an alternative technique that offers advantages such as complementary selectivity and faster separations when compared to RP HPLC. The columns in SFC are not as generic as in RP HPLC and therefore a screening of columns has to be performed prior to purification. This article describes how the Separation Science Laboratory at AstraZeneca R&D Molndal has selected five columns for SFC screen. The selection of columns has been validated by analyzing a large set of crude in-house compounds. The results show that when using the selected column set and a generic gradient, up to 90% of all in-house compounds may be purified using SFC-MS.


Vos J.D.,National Metrology Institute of South Africa | Quinn L.,National Metrology Institute of South Africa | Roos C.,North West University South Africa | Pieters R.,North West University South Africa | And 7 more authors.
TrAC - Trends in Analytical Chemistry | Year: 2013

We outline the experiences and the challenges of optimizing two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOF-MS) in conjunction with the H4IIE-. luc bio-assay for analyzing polychlorinated dibenzo-. p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in the South African context. Investigating such alternative analytical methods can assist countries with developing economies to meet their obligations under the Stockholm Convention. © 2013 Elsevier Ltd.


Fanali S.,National Research Council Italy | D'Orazio G.,National Research Council Italy | Lomsadze K.,Separation Science Laboratory | Samakashvili S.,Separation Science Laboratory | Chankvetadze B.,Separation Science Laboratory
Journal of Chromatography A | Year: 2010

Amylose tris(5-chloro-2-methylphenylcarbamate) was coated onto native and aminopropylsilanized silica in order to prepare chiral stationary phases (CSP) for enantioseparations using nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC). The effect of the nature of silica, the particle size and pore diameter, the chiral selector loading onto silica, the mobile phase composition and pH, as well as separation variables such as a linear flow rate of the mobile phase, applied voltage in CEC, etc. on the separation of enantiomers was studied. It was found that CSPs based on amylose tris(5-chloro-2-methylphenylcarbamate) can be used for preparation of stable capillary columns for enantioseparations by nano-LC and CEC in combination with polar organic and aqueous-organic mobile phases. Higher peak efficiency was observed in CEC than in nano-LC. © 2009 Elsevier B.V. All rights reserved.

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