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Ryu K.Y.,Research Policy Bureau | Lee M.-H.,NICS | Jung C.-S.,NICS | Yoon Y.,Sookmyung Womens University | And 3 more authors.
Korean Journal of Food Science and Technology | Year: 2012

This study was conducted to evaluate the bactericidal activity of weakly alkalic electrolyzed water (WEW) against Salmonella Typhimurium and Staphylococcus aureus on perilla leaves. The influences of organic matter, inform of bovine serum, and the ratio of WEW to perilla leaves on bactericidal activity of WEW were also examined. Treatment of these organisms with 25, 50, 75, and 100 ppm WEW was performed for 1 min, 3 min and 5 min, respectively. Higher bactericidal activity was observed after a treatment with 100 ppm WEW compared to a treatment with 25 ppm WEW by 0.7 log10CFU/g. The bactericidal activity of WEW also decreased with increasing bovine serum concentration. At the ratio of 10:1 (WEW: perilla leave), levels of Salmonella Typhimurium and Staphylococcus aureus were only reduced by 0.57 and 0.79 log10CFU/g, respectively. It is suggested that the removal of organic debris prior to application of sanitizers and treatment above the ratio of 25:1 (WEW: perilla leave) is needed in order to improve WEW activity. © The Korean Society of Food Science and Technology. Source


Sung I.K.,Gyeongin Regional Korea Food and Drug Administration | Park S.J.,Seoul Regional Korea Food and Drug Administration | Kang K.,Gyeongin Regional Korea Food and Drug Administration | Kim M.Y.,Jeju National University | Cho S.,Seoul National University
Korean Journal for Food Science of Animal Resources | Year: 2015

β-agonists are anabolic compounds that promote fat loss and muscle gain, and their administration to livestock may provide economic benefits by increasing growth rate and feed efficiency. For these reasons, β-agonists are also commonly added to livestock feed as growth promoters. This can introduce a significant risk of secondary human poisoning through intake of contaminated meat. A new method for the simultaneous determination of three β-agonists (clenbuterol, ractopamine, and zilpaterol) was developed in this study and applied to various meat samples. The limits of quantification, derived through a validation test following Codex guidelines, were 0.2 μg/kg for clenbuterol and zilpaterol, and 0.4 μg/kg for ractopamine. The average recoveries for clenbuterol, ractopamine, and zilpaterol ranged from 109.1% to 118.3%, 95.3% to 109.0%, and 94.1% to 120.0%, respectively. The recovery and coefficient of variation (CV) values fell within the acceptable range according to the Codex guidelines. This method reduced the analysis time without decreasing detection efficiency by modifying the pretreatment steps. This method could be utilized to manage the safety of imported meat products from countries where zilpaterol use is still permitted, thereby improving public health and preventing β-agonist poisoning due to secondary contamination. Source


Lee H.J.,Daegu Regional Korea Food and Drug Administration | Kang Y.W.,Daegu Regional Korea Food and Drug Administration | Lee S.M.,Daegu Regional Korea Food and Drug Administration | An K.A.,Daegu Regional Korea Food and Drug Administration | And 8 more authors.
Korean Journal of Food Science and Technology | Year: 2014

Penicillin and its salts, including the benzatine, procaine, and sodium salts, have been widely used in human and veterinary medicine. Owing to their low toxicity, they currently form the most important group of antibiotics. However, overdose and abuse of these antibiotics may lead to potential risk in human health. Therefore, this study was conducted to validate the analysis method established by the Korea Food Code in 2012 and to monitor the levels of benzylpenicillin residues in products with reference to the maximum residue level (MRL). Of the 232 product samples tested, benzylpenicillin was detected in 11 livestock products and 2 marine products. Benzylpenicillin concentrations were found to be lower than the MRL in 12 products; however, the concentration of benzylpenicillin was found to be greater than the MRL in 1 pork product. The limit of quantification (LOQ) for benzylpenicillin was found to be 0.001-0.002 mg/kg, with an average recovery of 90.4-115.3%. Calibrations showed good linearity of 0.995 over a range of 0.002-0.05 mg/kg. © The Korean Society of Food Science and Technology. Source


Kim H.Y.,Seoul Regional Korea Food and Drug Administration | Choi S.H.,Seoul Regional Korea Food and Drug Administration | Chung S.Y.,Seoul Regional Korea Food and Drug Administration | Choi H.J.,Seoul Regional Korea Food and Drug Administration | And 9 more authors.
Korean Journal of Food Science and Technology | Year: 2011

The purpose of this study was to develop a methodology to detect spinosad which are difficult to analyze by multi-component simultaneous analysis of pesticide residues. We monitored spinosad due to the paucity of related information. The spinosad was determined using HPLC with UV detector at 250 nm. Correlation coefficient (r2) for standard curve of spinosad A and D at standard concentration of 0.1-5.0 mg/kg were 0.999, respectively. Limit of quantitation (LOQ) of HPLC analysis was 0.005 mg/kg while limit of detection (LOD) was 0.001 mg/kg. Recovery experiments were conducted on five representative agricultural products to validate the analytical method. The recovery of proposed methods ranged from 74.9% to 104.0% and relative standard deviations were less than 10%. Spinosad residues were investigated in 16 commodities collected from 22 provinces. In this study, residues on all samples were not detected. © The Korean Society of Food Science and Technology. Source


Kim H.-Y.,Seoul Regional Korea Food and Drug Administration | Eom J.-Y.,Seoul Regional Korea Food and Drug Administration | Seo E.-C.,Seoul Regional Korea Food and Drug Administration | Choi S.-H.,Seoul Regional Korea Food and Drug Administration | And 2 more authors.
Korean Journal of Food Science and Technology | Year: 2010

To revise the dithiocarbamates residue analysis method and survey the residues in agricultural products that were treated with these fungicides in Korea, we purchased 20 types of foodstuffs (rice, potato, cabbage, apple etc.) from markets in five major cities. 236 samples of the purchased foodstuffs were then analyzed for the presence of dithiocarbamates by HPLC/UV and HPLC/APCI-MS. The R2, LOD and LOQ in the range of 0.5-107.3 mg/L were as follows: DCC: y=174.34x+18.315, R2=0.9999, 0.01 mg/L, and 0.04 mg/L; EBDC: y=227.38x-14.715, R2=1.0000, 0.01 mg/L and 0.02 mg/L; PBDC: y=38.46x-21.412, R2=0.9999, 0.04 mg/L, and 0.1 mg/L; ETU: y=52.752x-4.4819, R2=0.9-998, 0.02 mg/L and 0.03 mg/L; PTU: y=128.28x+4.4624, R2=0.9998, 0.02 mg/L, and 0.04 mg/L. The levels of DDC, EBDC, PBDC, ETU and PTU in 20 agricultural products fortified to 10.0-107.3 mg/L ranged from 61.7-117.5%, 65.3-110.1%, 61.5-109.6%, 69.3-116.3% and 70.2-97.2%, respectively. Overall, dithiocarbamates were detected in 100 samples and the detection ratio was 42.4%. Among these, only 3 samples (1.3%) of Lycii fructus had residue levels that were above the action limits, while the remaining samples (233 samples) contained levels of dithiocarbamates below the detection limit or below the Korea MRLs (Maximum Residue Limits). © The Korean Society of Food Science and Technology. Source

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