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Namakkal District, India

Ponmurugan P.,KS Rangasamy College of Technology | Manjukarunambika K.,KS Rangasamy College of Technology | Gnanamangai B.M.,Sengunthar Arts and Science College
Australasian Plant Pathology

The effect of various foliar diseases such as blister blight, brown blight, grey blight, bird’s eye spot and sooty mould on physiological functions, important biochemical, volatile and quality parameters of green and black teas were compared and evaluated. The results revealed that there was a considerable reduction in physiological activities such as photosynthetic and transpiration rates. Under natural condition, the diseases caused significant decrease in polyphenols, catechins, and aminoacids contents of green leaf which are known to be important precursors determining the quality of black tea. Consequently, a drastic variation existed between volatile flavor components of diseased leaves when compared to healthy ones. A corresponding decrease in the principal tea quality constituents like theaflavin and thearubigin were substantially reduced in made tea. Due to pathogen invasion in tea leaves, the degree of tea quality is varied in varying levels. Among the various tea pathogens, Exobasidium Vexans affected the overall tea quality extensively when compared to other tea pathogens. In addition, the infestation of diseases declined flavour index value and created difficulty in withering and fermentation during tea processing which ultimately affect the overall quality of tea. © 2016, Australasian Plant Pathology Society Inc. Source

Jayaprakashvel M.,University u s 3 of Act 1956 | Guru R.,Sengunthar Arts and Science College | Surendiran G.,University of Manitoba | Chelvan Y.,University u s 3 of Act 1956 | And 3 more authors.
Biosciences Biotechnology Research Asia

Probiotic bacteria are finding newer and wider applications in the food and allied industries. They were increasingly used as biopreservatives. Seafood processing and packaging is emerging as a strong industry now a days. In this context, probiotic organisms having the capacity to inhibit the pathogens in seafood shall have immediate applications. Hence, we have made a study on exploring potential probiotic lactobacillus and their antagonistic mechanisms against sea food pathogens and few human pathogens. Thirty different bacterial strains were isolated from commercial and house hold curd samples from Chennai. They were found to be Lactobacillus spp. based on their microscopic and biochemical properties. They were tested for their antimicrobial potential against common sea food pathogens viz., Salmonella sp., Shigella sp. and Vibrio sp. and human pathogens such as Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus. While screening for bactericidal activity, out of thirty, a strain Lactobacillus sp. AMET2017 exhibited remarkable inhibitory activity against one seafood and one human pathogens (maximum of 14 mm zone of inhibition) followed by Lactobacillus sp. AMET2025 in comparison with the other strains against both sea food and human pathogens. Crude ethyl acetate extracts (EAE) and crude bacteriocin (CB) were separated from the broth culture of Lactobacillus sp. AMET2017 and their Inhibitory Concentration (IC50) was tested using MTT 3- (4, 5- Dimethylthiazol-2-yl) - 2, 5- Diphenyltetrazolium bromide) assay. It has been found that the comparatively the EAE had better performance and hence it alone subjected to further studies. The antibacterial mechanism of EAE from Lactobacillus sp. AMET2017 was analyzed. There has been no considerable change in protein synthesis but the DNA integrity was found to be affected in the tested pathogens. Hence, it is concluded that the EAE of Lactobacillus sp. AMET2017 inhibits the sea food and human pathogens by affecting their DNA integrity and thus they can be effectively characterized for the use as biopreservatives of sea foods. Source

Suresh D.,Sengunthar Arts and Science College | Silambarasan T.,Periyar University | Dhandapani R.,Periyar University
International Journal of Pharma and Bio Sciences

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L - 1.5mg/L) and Indole acetic acid (IAA) (0.5mg/L - 1.5mg/L) for shoot induction. The best shoot proliferation was observed at the concentration of 1.0mg/L of BAP and 1.0 mg/L of IAA from only nodal explants of Canavalia gladiata after 4 weeks of culture. Combination of PGRs (BAP and IAA) was found to be more effective for shoot induction of nodal explants of Canavalia gladiata. For callus regeneration the nodal explants of Canavalia gladiata were used and the MS medium was supplemented with various concentration of a-naphthalene acetic acid (NAA) (0.5mg/L - 3.0mg/L). Highest callus induction was observed at the concentration of 1.5mg/L of NAA, and in this concentration highly desirable creamy white friable callus was observed. Source

Mohan Kumar A.,Government of Tamilnadu | Arumugam S.,Sengunthar Arts and Science College
International Journal of Pharma and Bio Sciences

Curvacin A, a type of bacteriocin produced by Lactobacillus curvatus LC05 has large spectrum of inhibition against human pathogenic, food spoilage microorganisms and many strains of Lactobacillus were isolated as a test organisms. The Curvacin A inhibited Enterococcus faecalis EF1 Escherichia coli NCTC 10418 but did not inhibit Candida albicans ATCC 10321. The antibacterial activity of the strains were observed between mid lograthmic and stationary phase of growth. Larger quantity of Curvacin A was found produced when the medium is supplemented with yeast extract (3.0%) sodium chloride (1.0-2.0%), glucose (1%) and Tween 80 (0.5%). Supplementation of sodium acetate, magnesium sulphate, tri ammonium citrate and dipotassium phosphate had no effect on production of Curvacin A. Source

Mohankumar A.,Government of Tamilnadu | Ranjitha P.,Sengunthar Arts and Science College
Indian Journal of Marine Sciences

Bioluminescence bacterium Vibrio fischeri was produces esterase enzyme when the medium contained specific substrate. Esterase was purified from the culture supernatant. Most active fractions were obtained using the technique of precipitation with 1N HCl. Precipitated fraction was purified by ion exchange chromatography (DEAE-Cellulose) and gel filtration chromatography (Sephadex G200). Enzyme purity was determined by RP-HPLC. Purified active fraction exhibiting final specific activity of 300U/mg and characterized; the optimum pH was 7.5, the optimum temperature was 30°C. Enzyme was very stable at the temperature 30°C and at wide range of pH. Enzyme was monomeric protein having molecular mass of 37 kDa estimated by native PAGE assay. Source

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