Entity

Time filter

Source Type


Qadeer S.,Pmas Arid Agriculture University | Khan M.A.,Pmas Arid Agriculture University | Ansari M.S.,University of Gujrat | Rakha B.A.,Pmas Arid Agriculture University | And 6 more authors.
Animal Reproduction Science | Year: 2015

Experiments were conducted to evaluate the effect of Antarctic fish antifreeze glycoproteins, (AFGP) size 1-5 (34-10.5kDa) and 7-8 (3.2 and 2.4kDa) in extender on buffalo bull sperm at cooling (4°C) and at post thawing. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina for 3 weeks. Qualifying ejaculates from each buffalo bull were diluted (at 37°C having 50×106sperm/mL) in tris-citric acid extender containing AFGP at 0 (control), 0.1, 1 and 10μg/mL. An aliquot of diluted semen was evaluated for sperm progressive motility and plasma membrane integrity, while the remaining fraction was cooled to 4°C in 2h. Further, an aliquot of cooled semen was evaluated for the previously described variables and the remaining fraction was cryopreserved (-196°C). After 24h of storage, straws were thawed at 37°C for 30s to assess post-thaw sperm quality. Inclusion of AFGP in the extender did not affect (P>0.05) sperm progressive motility and plasma membrane integrity of buffalo bull sperm at cooling stage (4°C). However, at post thawing, improvement (P<0.05) in sperm progressive motility and plasma membrane integrity was recorded in extender containing AFGP 1-5 and AFGP 7-8 at 1μg/mL compared to the control. Percentage of live sperm with an intact acrosome remained similar (P>0.05) in extenders containing different amounts of AFGP and control. In conclusion, supplementation of 1μg/ml of AFGP in extender improved the motility and plasma membrane integrity of Nili-Ravi buffalo sperm after thawing. © 2015 Elsevier B.V. Source


Qadeer S.,Pmas Arid Agriculture University | Khan M.A.,Pmas Arid Agriculture University | Ansari M.S.,University of Gujrat | Rakha B.A.,Pmas Arid Agriculture University | And 6 more authors.
Animal Reproduction Science | Year: 2014

Lower fertility in buffaloes with frozen-thawed semen is attributed to sperm damage that is believed to be due to formation of ice crystals during freeze/thaw process. It was hypothesized that antifreeze proteins in the extender may improve the post thaw quality of buffalo bull sperm. For this purpose, two separate experiments were conducted to evaluate antifreeze proteins III (AFP III) at 0 (control), 0.1, 1 and 10μg/mL (Experiment I) and 0 (control), 0.01, 0.1 and 1μg/mL (Experiment II) for its effect on post thaw quality of buffalo bull semen. Semen was collected from three Nili-Ravi buffalo (Bubalus bubalis) bulls with artificial vagina (42°C) for three weeks (replicate) per experiment. For each experiment, qualifying ejaculates (6ejaculates/bull) were divided into four aliquots and diluted (at 37°C having 50×106sperm/mL) in tris-citric acid extender containing above mentioned concentrations of AFP III. Diluted semen was cooled to 4°C in 2h, equilibrated for 4h, filled in 0.5mL straws, kept over liquid nitrogen vapors for 10min and plunged in the liquid nitrogen. After 24h of storage, semen straws were thawed at 37°C for 30s to assess sperm progressive motility (SM), plasma membrane integrity (PMI), viability (live sperm with intact acrosome) and normal epical ridge (NAR). In experiment I, improvement (P<0.05) in percentage SM and sperm PMI was recorded in extender containing 0.1μg/mL AFP III compared to control, the higher concentrations (1μg/mL and 10μg/mL) being inefficient. While evaluating the lower concentration (experiment II), 0.01μg/mL of AFP III in the extender it was found to be ineffective to improve semen quality parameters, while 0.1μg/mL AFP III in extender was found better in terms of progressive motility and plasma membrane integrity of buffalo bull semen compared to control. Sperm viability and NAR remained similar (P>0.05) in extenders containing different concentrations of AFP III and control in both of experiments. In conclusion addition of AFP III in the extender at 0.1μg/mL improved the progressive motility and plasma membrane integrity of cryopreserved buffalo bull semen. © 2014 Elsevier B.V. Source


Ahmad E.,University of Veterinary and Animal Sciences | Ahmad N.,University of Veterinary and Animal Sciences | Naseer Z.,University of Veterinary and Animal Sciences | Aleem M.,University of Veterinary and Animal Sciences | And 3 more authors.
Tropical Animal Health and Production | Year: 2011

The objective of the present study was to determine the relationship of age to body weight (BW), scrotal circumference (SC), number of pixels of testicular ultrasonograms (NP), and semen quality in Sahiwal bulls. The study was based on 128 Sahiwal bulls of different age groups (from 0 to >100 months of age). Bulls were evaluated for SC, BW, and NP. Semen was evaluated once a week for five consecutive weeks from regularly collected donor bulls (n = 86) ranging in age from 25-30 to >100 months. Ejaculate volume, sperm motility, sperm concentration, sperm morphology, percent live sperms, sperm plasma membrane integrity, and normal acrosome were compared among different age groups. Mean SC and BW increased (P < 0.05) in a curvilinear manner from birth to >100 months of age. Mean NP of testicular ultrasonograms increased (P < 0.05) from 0 to 24 months and then plateaued until >100 months of age. Body weight, SC, and NP were positively correlated with age from birth until >100 months (r = 0.91, 0.87, and 0.40, respectively). Ejaculate volume (5.7 ± 0.2 vs. 4.6 ± 0. 09 ml) and sperm concentration (1,281.6 ± 17. 7 vs. 1,115. 8 ± 55. 9 × 106/ml) increased (P < 0.05) in mature bulls compared to younger ones. However, motility (68.6 ± 0.3%), plasma membrane integrity (50.8 ± 1.0%), and normal acrosome (74.8 ± 0.5%) remained insignificant due to age. In six of eight age groups studied, morphological abnormalities were well within the range (18.1 ± 0.3%). In conclusion, the BW, SC, and NP of testicular ultrasonograms, ejaculate volume, and concentration increased with age. Moreover, semen quality is fairly independent of age except volume and concentration in Sahiwal bulls. © 2010 Springer Science+Business Media B.V. Source

Discover hidden collaborations