Selye Janos University in Komarno

Komárno, Slovakia

Selye Janos University in Komarno

Komárno, Slovakia
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PubMed | University of Medicine and Pharmacy, Cluj-Napoca, Selye Janos University in Komarno, Laboratory of Veterinary Histopathology in Komarno, Czech University of Life Sciences and 3 more.
Type: Comparative Study | Journal: Pathology, research and practice | Year: 2014

Non-alcoholic-fatty-liver-disease (NAFLD) is a clinicopathologic entity characterized by a variety of hepatic injury patterns without significant alcohol use. It has a close association with obesity, so treatment includes weight loss, control of insulin sensitivity, interventions directed at inflammation and fibrosis. There is a certain relationship between the grade and duration of food restriction and hepatic function. The objective of this work was to describe the relationship between biochemistry, autoantibodies, insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein 3 (IGFBP-3), and liver morphology in experimental rabbit groups with food restriction as compared to controls with ad libitum food (ADL) income. The experiment was performed on a total of 24 rabbits of a weaning age of 25-81 days. The first group (R1) was restricted between 32 and 39 days of age to 50 g of food per rabbit a day. The second group (R2) was also restricted between 32 and 39 days, but the rabbits received 65 g of food per rabbit a day. At the end of the experiment, the blood and liver samples were collected at necropsy. NAFLD has developed in all three groups. There was any autoantibody positivity in all three groups. IGF-I is moderately higher in R1 and R2 group, as compared to the control group (P > 0.05). IGFBP-3 is without statistical significance in all three groups. Alkaline phosphatase (ALP) is the only liver biochemical parameter that has significantly increased following food restriction (P > 0.039). Single one-week restriction has any protective effect on NAFLD development.


Makovicky P.,Academy of Sciences of the Czech Republic | Kopecky O.,Czech University of Life Sciences | Makovicky P.,Selye Janos University in Komarno | Matlach R.,Forensic Expert in the Field of Healthcare
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2015

Skeletochronology is a widely used method for age determination in amphibians. This method is based mainly on the histological examination of the finger bones. However, the lengths of utilized severed fingers have not been specified in previous studies. The objective of this study was to analyse the structure of line arrested growth (LAGs) involving taking only the last two phalanges of a finger, and using the entire finger of Alpine newts (Mesotriton alpestris). Altogether 432 fingers were taken from four localities in the Czech Republic during the newt breeding period. The first group (group A) contained fingers that consisted of the last two phalanges (330 samples), and the second (group B) contained complete fingers with all phalanges (102 samples). All fingers were processed using standard histological methods and stained with hematoxylin-eosin. Phalange cross-section slides were made, and the ages of the individuals were determined by the number of LAGs. From two phalanges it is determine the age of 17.87% of newts; however, age determination was successful in 49.01% of newts when using whole fingers. Age determination success rate differences between groups were significant (P < 0.001). This is a histological study and it is recommended utilizing whole fingers in future Caudata amphibian screening age studies. © 2015, Mendel University of Agriculture and Forestry Brno. All rights reserved.


Makovicky P.,Laboratory of Veterinary Histopathology in Komarno | Tumova E.,Czech University of Life Sciences | Volek Z.,Institute of Animal Science in Prague Uhrineves | Makovicky P.,Selye Janos University in Komarno | And 10 more authors.
Pathology, research and practice | Year: 2014

Non-alcoholic-fatty-liver-disease (NAFLD) is a clinicopathologic entity characterized by a variety of hepatic injury patterns without significant alcohol use. It has a close association with obesity, so treatment includes weight loss, control of insulin sensitivity, interventions directed at inflammation and fibrosis. There is a certain relationship between the grade and duration of food restriction and hepatic function. The objective of this work was to describe the relationship between biochemistry, autoantibodies, insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein 3 (IGFBP-3), and liver morphology in experimental rabbit groups with food restriction as compared to controls with ad libitum food (ADL) income. The experiment was performed on a total of 24 rabbits of a weaning age of 25-81 days. The first group (R1) was restricted between 32 and 39 days of age to 50 g of food per rabbit a day. The second group (R2) was also restricted between 32 and 39 days, but the rabbits received 65 g of food per rabbit a day. At the end of the experiment, the blood and liver samples were collected at necropsy. NAFLD has developed in all three groups. There was any autoantibody positivity in all three groups. IGF-I is moderately higher in R1 and R2 group, as compared to the control group (P > 0.05). IGFBP-3 is without statistical significance in all three groups. Alkaline phosphatase (ALP) is the only liver biochemical parameter that has significantly increased following food restriction (P > 0.039). Single one-week restriction has any protective effect on NAFLD development. Copyright © 2014 Elsevier GmbH. All rights reserved.

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