Kitakyushu, Japan

Seinan Jo Gakuin University

www.seinan-jo.ac.jp
Kitakyushu, Japan

Seinan Jo Gakuin University is a private women's college in Kitakyushu, Fukuoka, Japan. The predecessor of the school was founded in 1922, and it was chartered as a university in 1994. Wikipedia.


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Okada K.,Fukuoka National Hospital | Miyazaki C.,Fukuoka West Rehabilitation Center for Children | Kino Y.,Chemo Sero Therapeutic Research Institute Kaketsuken | Ozaki T.,Konan Kosei Hospital | And 2 more authors.
Journal of Infectious Diseases | Year: 2013

Background. Phase II and III clinical studies were conducted to evaluate immunogenicity and safety of a novel DTaP-IPV vaccine consisting of Sabin inactivated poliovirus vaccine (sIPV) and diphtheria-tetanus-acellular pertussis vaccine (DTaP).Methods. A Phase II study was conducted in 104 healthy infants using Formulation H of the DTaP-sIPV vaccine containing high-dose sIPV (3, 100, and 100 D-antigen units for types 1, 2, and 3, respectively), and Formulations M and L, containing half and one-fourth of the sIPV in Formulation H, respectively. Each formulation was administered 3 times for primary immunization and once for booster immunization. A Phase III study was conducted in 342 healthy infants who received either Formulation M + oral polio vaccine (OPV) placebo or DTaP + OPV. The OPV or OPV placebo was orally administered twice between primary and booster immunizations.Results. Formulation M was selected as the optimum dose. In the Phase III study, the seropositive rate was 100% for all Sabin strains after primary immunization, and the neutralizing antibody titer after booster immunization was higher than in the control group (DTaP + OPV). All adverse reactions were clinically acceptable.Conclusions. DTaP-sIPV was shown to be a safe and immunogenic vaccine.Clinical Trials Registration. JapicCTI-121902 for Phase II study, JapicCTI-101075 for Phase III study (http://www.clinicaltrials.jp/user/cte-main.jsp). © 2013 The Author 2013. All rights reserved.


Miyazaki C.,Fukuoka West Rehabilitation Center for Children | Okada K.,Fukuoka Dental College | Ozaki T.,Konan Kosei Hospital | Hirose M.,Hirose Pediatric Clinic | And 5 more authors.
Clinical and Vaccine Immunology | Year: 2014

The immunogenicity and safety of an inactivated cell culture Japanese encephalitis vaccine (CC-JEV) were compared with those of an inactivated mouse brain-derived Japanese encephalitis vaccine (MB-JEV) in phase III clinical multicenter trials conducted in children. The vaccines contain the same Japanese encephalitis virus strain, the Beijing-1 strain. Two independent clinical trials (trials 1 and 2) were conducted. Trial 1 was conducted in 468 healthy children. Each subject was injected with 17 μg per dose of either CC-JEV or MB-JEV, and the immunogenicity and safety of the vaccines were investigated. Trial 1 showed that CC-JEV was more immunogenic and reactive than MB-JEV at the same dose. Therefore, to adjust the immunogenicity of CC-JEV to that of MB-JEV, a vaccine that has had a good track record regarding its efficacy for a long time, trial 2 was conducted in 484 healthy children. To improve the stability, CC-JEV was converted from a liquid type to a freeze-dried type of vaccine. Each subject was injected subcutaneously with either 4 μg per dose of CC-JEV, 8 μg per dose of CC-JEV, or 17 μg per dose of MB-JEV twice, at an interval of 2 to 4 weeks, followed by an additional booster immunization 1 to 15 months after the primary immunization. Based on the results of trial 2, 4 μg per dose of the freeze-dried CC-JEV (under the label Encevac) was selected as a substitute for the MB-JEV. Encevac was approved and launched in 2011 and has since been in use as a 2nd-generation Japanese encephalitis vaccine in Japan. (These studies have been registered at the JapicCTI under registration no. JapicCTI-132063 and JapicCTI-080586 for trials 1 and 2, respectively.) Copyright © 2014, American Society for Microbiology. All Rights Reserved.


Azekoshi Y.,University of Ryukyus | Yasu T.,University of Ryukyus | Watanabe S.,University of Ryukyus | Tagawa T.,University of Ryukyus | And 7 more authors.
Hypertension | Year: 2010

Release of free fatty acid (FFA) from adipose tissue is implicated in insulin resistance and endothelial dysfunction in patients with visceral fat obesity. We demonstrated previously that increased FFA levels cause endothelial dysfunction that is prevented by inhibition of the renin-angiotensin system (RAS) in humans. However, the mechanisms for FFA-mediated activation of RAS and the resultant endothelial dysfunction were not elucidated. We investigated effects of elevated FFA on activity of circulating and vascular RAS, angiotensin II-forming activity of leukocytes, and leukocyte activation of normotensive subjects. We showed that increased FFA levels significantly enhanced angiotensin II-forming activity in human mononuclear (mean fold increase: 3.5 at 180 minutes; P=0.0016) and polymorphonuclear (2.0; P=0.0012) cells, whereas parameters of the circulating and vascular RAS were not affected. We also showed that FFA caused angiotensin II-dependent leukocyte activation, which impaired endothelial function partly via increased myeloperoxidase release and presumably enhanced adhesion of leukocytes. We propose that the enhanced production of angiotensin II by FFA in mononuclear and polymorphonuclear cells causes activation of leukocytes that consequently impairs endothelial function. RAS in leukocytes may regulate the leukocyte-vasculature interaction as the mobile RAS in humans. © 2010 American Heart Association, Inc.


Abdel-Rahman M.A.,Kyushu University | Abdel-Rahman M.A.,Al - Azhar University of Egypt | Tashiro Y.,Seinan Jo Gakuin University | Sonomoto K.,Kyushu University
Journal of Biotechnology | Year: 2010

Lactic acid is an industrially important product with a large and rapidly expanding market due to its attractive and valuable multi-function properties. The economics of lactic acid production by fermentation is dependent on many factors, of which the cost of the raw materials is very significant. It is very expensive when sugars, e.g., glucose, sucrose, starch, etc., are used as the feedstock for lactic acid production. Therefore, lignocellulosic biomass is a promising feedstock for lactic acid production considering its great availability, sustainability, and low cost compared to refined sugars. Despite these advantages, the commercial use of lignocellulose for lactic acid production is still problematic. This review describes the " conventional" processes for producing lactic acid from lignocellulosic materials with lactic acid bacteria. These processes include: pretreatment of the biomass, enzyme hydrolysis to obtain fermentable sugars, fermentation technologies, and separation and purification of lactic acid. In addition, the difficulties associated with using this biomass for lactic acid production are especially introduced and several key properties that should be targeted for low-cost and advanced fermentation processes are pointed out. We also discuss the metabolism of lignocellulose-derived sugars by lactic acid bacteria. © 2011 Elsevier B.V..


Abdel-Rahman M.A.,Kyushu University | Abdel-Rahman M.A.,Al - Azhar University of Egypt | Tashiro Y.,Seinan Jo Gakuin University | Zendo T.,Kyushu University | And 3 more authors.
Applied and Environmental Microbiology | Year: 2011

Enterococcus mundtii QU 25, a newly isolated lactic acid bacterium, efficiently metabolized xylose into L-lactate. In batch fermentations, the strain produced 964 mM L-(+)-lactate from 691 mM xylose, with a yield of 1.41 mol/mol xylose consumed and an extremely high optical purity of ≥99.9% without acetate production. © 2011, American Society for Microbiology.


Tanaka S.,Saga University | Tanaka S.,Osaka Municipal Technical Research Institute | Tashiro Y.,Seinan Jo Gakuin University | Tashiro Y.,Kyushu University | And 4 more authors.
Bioresource Technology | Year: 2012

A polytetrafluoroethylene (PTFE) membrane was used in membrane-assisted extractive (MAE) fermentation of acetone-butanol-ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4. The growth inhibition effects of 1-dodecanol, which has a high partition coefficient for butanol, can be prevented by employing 1-dodecanol as an extractant when using a PTFE membrane. Compared to conventional fermentation, MAE-ABE fermentation with 1-dodecanol decreased butanol inhibition and increased glucose consumption from 59.4 to 86.0g/L, and total butanol production increased from 16.0 to 20.1g/L. The maximum butanol production rate increased from 0.817 to 0.979g/L/h. The butanol productivity per membrane area was remarkably high with this system, i.e., 78.6g/L/h/m 2. Therefore, it is expected that this MAE fermentation system can achieve footprint downsizing. © 2012 Elsevier Ltd.


Baba S.-I.,Kyushu University | Tashiro Y.,Seinan Jo Gakuin University | Shinto H.,Kyushu University | Sonomoto K.,Kyushu University
Journal of Biotechnology | Year: 2012

Living cells are alive and have the butanol-producing ability but not much proliferation under nitrogen source-limited condition. We investigated various butanol production systems with high density of living cells of Clostridium saccharoperbutylacetonicum N1-4 supplemented with methyl viologen (MV) as an electron carrier and nutrient dosing for activity regeneration. In continuous butanol production with high density of living cells, butanol yield was drastically increased from 0.365C-mol/C-mol with growing cells to 0.528C-mol/C-mol at a dilution rate of 0.85h -1, being increased with the butanol to total solvent ratio. This yield was increased to 0.591C-mol/C-mol by adding 0.01mM MV. MV addition increased not only butanol yield but also butanol concentration and productivity as compared to those without MV addition. However, living cells lost their activity with incubation time, which lowered the operational stability of the system. Therefore, to maintain constant stability, activity regeneration was carried out with high density of living cells and MV. This system produced butanol at high concentration (9.40gl -1) and productivity (7.99gl -1h -1) for approximately 100h with maintenance of considerably high yield of butanol (0.686C-mol/C-mol). Thus, we established a high-speed and highly efficient butanol production system. © 2011 Elsevier B.V.


Sato Y.,Seinan Jo Gakuin University | Fukahara A.,Seinan Jo Gakuin University
[Nihon kōshū eisei zasshi] Japanese journal of public health | Year: 2015

OBJECTIVES: It is important that commissioned child welfare volunteers (CCWVs) fully understand the needs, objectives, and prospective results of working on the Visits to All Families with Infants Program. This study aimed to explore the factors that enhanced volunteers' recognition of the need for the program and to reveal issues that need to be addressed to improve support for CCWVs.METHODS: All 259 CCWV who were engaged in the Visits to All Families with Infants Program and who were living in Kitakyushu City, Japan, participated in the study between August and December, 2013. We questioned the volunteers about their recognition of the need for the program, asking, "Do you think that it is necessary for CCWVs to visit families with infants four months of age or younger?" In addition, we asked about their demographic characteristics, experience in the program, and activities aside from the program. Their recognition was measured with scores on a 5-point scale, and the score was divided into 1 (good recognition) or 0 (poor recognition). Multiple logistic regression was used for statistical analysis and to calculate the odds ratio (OR) and 95% confidence interval (CI). Participants who had a "good experience" in the program were asked to explain these experiences by free description and these were analyzed qualitatively using content analysis.RESULTS: The response rate of the study was 92.1%, and the final analysis involved 154 CCWV (59.5%). Good recognition was ascertained for 69 individuals (44.8%) and poor recognition for 85 individuals (55.2%). Statistical analysis revealed that the volunteers' recognition of the need for the program was associated with qualifications related to medicine, health, or welfare (OR: 2.57, 95% CI: 1.12-6.20), and whether the volunteers had a "good experience" when they visited families (OR: 18.35, 95% CI: 6.41-67.60). Regarding the content analysis of "good experience," 2 main categories, 7 categories, and 18 sub-categories were outlined.CONCLUSION: This study indicated that if CCWVs have a qualification or good experience through the program, then their awareness of the social needs to work in the Visits to All Families with Infants Program would be strengthened.


Sonomoto K.,Kyushu University | Oshiro M.,Kyushu University | Hanada K.,Kyushu University | Tashiro Y.,Seinan Jo Gakuin University
Applied Microbiology and Biotechnology | Year: 2010

In order to achieve high butanol production by Clostridium saccharoperbutylacetonicum N1-4, the effect of lactic acid on acetone-butanol-ethanol fermentation and several fed-batch cultures in which lactic acid is fed have been investigated. When a medium containing 20 g/l glucose was supplemented with 5 g/l of closely racemic lactic acid, both the concentration and yield of butanol increased; however, supplementation with more than 10 g/l lactic acid did not increase the butanol concentration. It was found that when fed a mixture of lactic acid and glucose, the final concentration of butanol produced by a fed-batch culture was greater than that produced by a batch culture. In addition, a pH-controlled fed-batch culture resulted in not only acceleration of lactic acid consumption but also a further increase in butanol production. Finally, we obtained 15.5 g/l butanol at a production rate of 1.76 g/l/h using a fed-batch culture with a pH-stat continuous lactic acid and glucose feeding method. To confirm whether lactic acid was converted to butanol by the N1-4 strain, we performed gas chromatography-mass spectroscopy (GC-MS) analysis of butanol produced by a batch culture during fermentation in a medium containing [1,2,3-13C3] lactic acid as the initial substrate. The results of the GC-MS analysis confirmed the bioconversion of lactic acid to butanol. © Springer-Verlag 2010.


Tashiro Y.,Seinan Jo Gakuin University | Kaneko W.,Kyushu University | Sun Y.,Kyushu University | Shibata K.,Kyushu University | And 3 more authors.
Applied Microbiology and Biotechnology | Year: 2011

We isolated and characterized a d-lactic acid-producing lactic acid bacterium (d-LAB), identified as Lactobacillus delbrueckii subsp. lactis QU 41. When compared to Lactobacillus coryniformis subsp. torquens JCM 1166T and L. delbrueckii subsp. lactis JCM 1248T, which are also known as d-LAB, the QU 41 strain exhibited a high thermotolerance and produced d-lactic acid at temperatures of 50°C and higher. In order to optimize the culture conditions of the QU 41 strain, we examined the effects of pH control, temperature, neutralizing reagent, and initial glucose concentration on d-lactic acid production in batch cultures. It was found that the optimal production of 20.1 g/l d-lactic acid was acquired with high optical purity (>99.9% of d-lactic acid) in a pH 6.0-controlled batch culture, by adding ammonium hydroxide as a neutralizing reagent, at 43°C in MRS medium containing 20 g/l glucose. As a result of product inhibition and low cell density, continuous cultures were investigated using a microfiltration membrane module to recycle flow-through cells in order to improve d-lactic acid productivity. At a dilution rate of 0.87 h-1, the high cell density continuous culture exhibited the highest d-lactic acid productivity of 18.0 g/l/h with a high yield (ca. 1.0 g/g consumed glucose) and a low residual glucose (<0.1 g/l) in comparison with systems published to date. © 2010 Springer-Verlag.

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