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Gimcheon, South Korea

Sim S.-C.,Sejong University | Hong J.-H.,Seed Testing & Research Center | Kwon Y.-S.,Dong - A University
Horticulture Environment and Biotechnology | Year: 2015

Pumpkin (Cucurbita spp.) is a major vegetable crop grown worldwide. Three species, C. pepo, C. moschata, and C. maxima, are economically important cultivated pumpkins. To develop a core set of markers for DNA profiling and cultivar identification, we used a total of 300 SSRs consisting of 158 CMTp and 142 CMTm that were previously identified in C. pepo and C. moshata, respectively. Polymorphisms in these primers were tested using a subset of 22 cultivars selected from a collection of 160 commercial cultivars. A total of 12 CMTp and 28 CMTm markers were selected based on polymorphism and number of alleles, and these 40 markers were used to genotype all 160 cultivars. Of these, 29 markers (5 CMTp and 24 CMTm) accurately detected a total of 215 alleles with an average of 7.41 alleles per marker in our collection of pumpkin cultivars. Their PIC values ranged from 0.327 to 0.894 with an average of 0.674. Analysis of genetic similarity using the 29 SSR markers revealed that the 160 cultivars were divided into five major clusters representing C. maxima×C. moshata hybrids (cluster I), C. moshata (cluster II), C. maxima (cluster III), C. pepo (cluster IV), and C. ficifolia (cluster V). In clusters I-IV, the cultivars were further separated into 2-3 sub-clusters. In addition, we found that 29 SSR markers were able to differentiate all 160 cultivars. Results from our study will facilitate genetic study and protection of breeders’ intellectual property rights in pumpkins. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH. Source

Sim S.-C.,Sejong University | Hong J.-H.,Seed Testing & Research Center | Choi K.-J.,South Korean National Institute of Animal Science | Kim D.-H.,Dong - A University | Kwon Y.-S.,Dong - A University
Horticulture Environment and Biotechnology | Year: 2015

Radish (Raphanus sativus L.) is a major vegetable cultivated worldwide. As a member of the family Brassicaceae, this species has diverse morphological characteristics in root. Radish cultivars have been classified based on morphological traits, including root shape and color. Despite its economic importance in Asia, genomic research in radish is less well developed relative to Brassica rapa, a close relative of radish. In this study, we developed genomic simple sequence repeat (SSR) markers using a SSR-enriched library and investigated genetic diversity in a collection of 144 radish cultivars. A total of 237 primer pairs for SSRs were designed and 184 (77.6%) primer pairs produced PCR amplicons. Of these, we selected 27 SSR markers (14.7%) based on polymorphism in a subset of 11 cultivars and then used to assess genetic relationships in the germplasm panel. For these markers, the number of alleles per marker ranged from 2 to 18 with an average of 7.77 alleles and the polymorphism information content (PIC) values ranged from 0.491 to 0.906. The estimates of pairwise Fst revealed significant genetic differentiation between the five market classes of 135 radish cultivars (74 big, 15 small, 29 young, 12 Altari, and 5 processing). Clustering analysis using NTSYS-pc and STRUCTURE software also found that the 74 big radishes were divided into 5–7 clusters. In addition, all 27 SSR markers were able to differentiate 64 big radish cultivars based on the UPGMA dendrogram and each of the 23 markers independently identified 1 to 17 big radish cultivars. These results suggest that cultivated radishes have different patterns of genetic variation and breeding practices should be a driving force for the genetic differentiation between and within market classes. The SSR markers developed in this study will be a useful resource for genetic study and protection of plant breeder’s intellectual property right through cultivar identification. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH. Source

Soh E.H.,Seed Testing & Research Center | Lee W.M.,National Institute of Horticultural and Herbal Science | Park K.W.,Chungnam National University | Choi K.J.,National Institute of Horticultural and Herbal Science | Yoon M.K.,Seed Testing & Research Center
Korean Journal of Horticultural Science and Technology | Year: 2014

Seed viability is affected by storage conditions and rapid loss of viability in storage is the major cause for low germination. This study was carried out to examine the effect of packaging materials and storage temperature on seed germination rate over 10 years in two species (Capsicum annuum L. and Brassica rapa L. ssp. pekinensis) and determine effective storage conditions for maintaining seed viability. Seeds were packaged in aluminum poly pouches under vacuum, polyethylene bottles, and paper bags containing silica gel and stored under one of two controlled conditions (15°C, RH 40% or 5°C, RH 30%) or at ambient condition. Seed germination was recorded at 6-month intervals for 10 years. The seeds of both species showed no decline in viability until 6.5 years at 15 or 5°C, irrespective of packaging materials. However, under ambient conditions, the seeds of chili pepper and Chinese cabbage in paper bags lost viability after 4 and 5 years, respectively. By contrast, seeds of both species in vacuum-aluminum poly pouches exhibited a 99% germination rate after 6 years under ambient conditions. Pepper seeds in the vacuum-aluminum poly pouches maintained a 93% germination rate after 10 years in ambient conditions. These results indicated that a special seed storage facility for maintaining viability of chili pepper and Chinese cabbage seed might not be essential and seed testing would not be necessary for 10 years, if chili pepper and Chinese cabbage seeds were packed in ambient/vacuum-aluminum poly pouches or 5°C/vacuum-aluminum poly pouches. © 2014 Korean Society for Horticultural Science. Source

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