Entity

Time filter

Source Type


Bakooie M.,Tarbiat Modares University | Pourjam E.,Tarbiat Modares University | Mahmoudi S.B.,Sugar Beet Seed Institute SBSI | Safaie N.,Tarbiat Modares University | Naderpour M.,Seed and Plant Certification and Registration Institute SPCRI
Journal of Agricultural Science and Technology | Year: 2015

Linked and/or gene-based molecular markers have been used widely in markerassisted selection (MAS) to differentiate resistant and susceptible genotypes. Resistance to Meloidogyne spp. in Beta vulgaris L. is mediated by a single dominant gene (R6m-1). Using allele-specific primers (ASPs), an SNP marker harboring a single nucleotide polymorphism (A/G), linked to the resistance gene was developed to differentiate resistant genotypes. The differentiation among the resistant and susceptible genotypes was elucidated in the polymorphic bands of 555, 478 and 124 bp in size, using PCR amplification. The genotyping data using the SNP marker was firmly associated with the bioassay evaluation in the greenhouse for 100 sugar beet genotypes. This data indicated that the present robust marker allowed reliable, sensitive, faster, and cheaper large scale screening of B. vulgaris genotypes for nematode resistance breeding programs. © 2015, Tarbiat Modares University. All rights reserved. Source


Ahmadi M.,Islamic Azad University at Tehran | Heravan E.M.,Islamic Azad University at Tehran | Sadeghian S.Y.,Seed and Plant Certification and Registration Institute SPCRI | Mesbah M.,Seed and Plant Certification and Registration Institute SPCRI | Darvish F.,Islamic Azad University at Tehran
Euphytica | Year: 2011

Drought tolerance is one of the most important objectives of sugar beet breeding programs in semi-arid regions, particularly during the last decade. Due to global climate changes and limitations of agricultural irrigation water, varieties with drought tolerance are taken into consideration in order to avoid yield losses due to drought. In this study, drought tolerance of 76 S1 lines (full-sib families) that had been extracted from a genetically broad base multigerm sugar beet open pollinated population, were examined. Test crosses were made between the lines as pollinators and a cytoplasmic male sterile (CMS) single cross. The consequent hybrids along with checks were evaluated during 2007 and 23 more tolerant hybrids during 2008, in two adjacent experiments under drought stress and non-stress conditions. Drought tolerance indices calculated based on sugar yield, such as mean productivity (MP), geometric mean productivity (GMP) and stress tolerance index (STI) were used to assess hybrids responses to drought. The results showed significant genetic differences for root yield and sugar yield under both conditions. Drought tolerance indices displayed significant genetic variability for sugar yield among the hybrids. Many hybrids were drought tolerant as compared with the original base population as indicated by their high STI. The estimates of heritability for sugar yield in stress and non-stress conditions were much close to each other (0.31 and 0.34, respectively). Whereas, for root yield the heritability estimate in stress condition (0.46) was relatively higher than that in non-stress condition (0.34). Significant differences were observed among the selected hybrids for root yield and sugar yield, indicating genotypic variability for pollinator lines derived from the population. There were no significant differences for sugar content. For increasing the drought tolerance potential in a breeding population and developing drought-tolerant varieties by male parent, the drought-tolerant lines could be used. © 2010 Springer Science+Business Media B.V. Source


Zareian A.,Seed and Plant Certification and Registration Institute SPCRI | Yari L.,Seed and Plant Certification and Registration Institute SPCRI | Hasani F.,Seed and Plant Certification and Registration Institute SPCRI | Ranjbar G.H.,Iranian National Salinity Research Center
World Applied Sciences Journal | Year: 2012

In order to evaluate the effect of seed size on emergence, yield and components yield of three bread wheat (Triticum aestivum L.) cultivars, an experiment was conducted at Seed and Plant Certification and Registration Research Institute (SPCRI), Karaj, Iran. The experimental factors included five seed sizes (2-2.2, 2.2-2.5, 2.5-2.8, 2.8-3 and >3 mm) and three wheat cultivars (Mahdavi, Pishtaz and Bahar). The experiment was carried out in a 3×5 factorial design (three cultivars, five different seed sizes) based on randomized complete blocks with three replicates. Seedling emergence percentage, green weight of 100 plants, dry weight of 100 plants, the number of spikes per area, number of seeds per spike, 1000-seeds weight and grain yield were measured. Seed size had a significant impact on all of measured traits with the exception of 1000-seeds weight. Results indicated that number of seeds per spike significantly decreased by increasing seed size. The other traits showed significant increase by increasing seed size. Cultivar had significant effect on 1000-seeds weight and number of spikes per area but other traits were similar among cultivars. This study suggested that large seed sizes were superior compared to the other seed size and wheat cultivars had similar performance regarding to the variation in seed sizes. No significant interaction was observed for all traits studied in this experiment. © IDOSI Publications, 2012. Source


Pourabed E.,Islamic Azad University at Miyaneh | Jazayeri Noushabadi M.R.,Seed and Plant Certification and Registration Institute SPCRI | Jamali S.H.,Seed and Plant Certification and Registration Institute SPCRI | Moheb Alipour N.,Islamic Azad University at Miyaneh | And 2 more authors.
International Journal of Plant Genomics | Year: 2015

Identification and registration of new rice varieties are very important to be free from environmental effects and using molecular markers that are more reliable. The objectives of this study were, first, the identification and distinction of 40 rice varieties consisting of local varieties of Iran, improved varieties, and IRRI varieties using PIC, and discriminating power, second, cluster analysis based on Dice similarity coefficient and UPGMA algorithm, and, third, determining the ability of microsatellite markers to separate varieties utilizing the best combination of markers. For this research, 12 microsatellite markers were used. In total, 83 polymorphic alleles (6.91 alleles per locus) were found. In addition, the variation of PIC was calculated from 0.52 to 0.9. The results of cluster analysis showed the complete discrimination of varieties from each other except for IR58025A and IR58025B. Moreover, cluster analysis could detect the most of the improved varieties from local varieties. Based on the best combination of markers analysis, five pair primers together have shown the same results of all markers for detection among all varieties. Considering the results of this research, we can propose that microsatellite markers can be used as a complementary tool for morphological characteristics in DUS tests. © 2015 Ehsan Pourabed et al. Source


Zareian A.,Seed and Plant Certification and Registration Institute SPCRI | Hamidi A.,Seed and Plant Certification and Registration Institute SPCRI | Sadeghi H.,Seed and Plant Certification and Registration Institute SPCRI | Jazaeri M.R.,Seed and Plant Certification and Registration Institute SPCRI
Middle East Journal of Scientific Research | Year: 2013

The effect of seed size on germination, emergence percentage and yield o f three bread wheat (Triticum aestivum L.) cultivars were evaluated in Seed and Plant Certification and Registration Research Institute (SPCRI), Karaj, Iran. The experiment factors included five seed sizes (2-2.2, 2.2-2.5, 2.5-2.8, 2.8-3 and >3 mm) of three wheat cultivars (Mahdavi, Pishtaz and Bahar). Seed size had significant impact on all measured traits in laboratory and field with the exception of germination percentage and harvest index. Results indicated that germination rate significantly decreased by increasing seed size. The other traits showed significant increase by increasing seed size. Mahdavi cultivar had significant effect on seedling dry weight and dry weight of 100 plants. Other traits were similar among cultivars. Significant interaction was observed for seedling dry weight and dry weight of 100 plants. Assessment of treatments in this study showed that seed size had no significant impact on germination percentage, but it changes seedling emergence and grain yield, in this way the best category of seed size was related to >2.2-2.5 size, whereas emergence percentage and yield of seeds with 2-2.2 size was significantly less than other sizes. © IDOSI Publications, 2013. Source

Discover hidden collaborations