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Sharafizad M.,Seed and Plant Certification and Registration Institute | Lak S.,Islamic Azad University | Naghashzadeh M.,Khorramabad Payamenoor University
Advances in Environmental Biology

In order to study effects of salicylic acid and water stress on proline accumulation and cell membrane stability of wheat (Triticum aestivum L.) cultivar Chamran, two field experiments were conducted in 2012 and 2013. The results of the combined analysis showed that year has not significantly affected measured traits. Water stress has significantly affected cell membrane stability and leaf proline accumulation. The highest leaf proline accumulation was shown in grain filling stage under water stress and low dosage of salicylic acid application in generative stage. Salicylic acid application increase proline accumulation in vegetative, generative stages and decrease electrolyte leakage. These processes lead to increase yield against detrimental effects of water stress. © 2013 AENSI Publisher All rights reserved. Source

Baghaee Ravari S.,Ferdowsi University of Mashhad | Moslemkhani K.,Seed and Plant Certification and Registration Institute | Khodaygan P.,Vali r University
Journal of Plant Pathology

Pectobacterium carotovorum subsp. carotovorum (Pcc) is the causal agent of soft rot of potato tubers. This study was conducted to assess the genetic variation of a collection of 32 Pcc isolates from three different potato cultivars grown in the eastern parts of Iran using subspecies- specific PCR, 16S rDNA sequencing and rep- PCR analyses and comparing these methods with each other. In initial experiments, the identity of isolates was confirmed by specific primers related to Pcc subspecies and differentiated from closely species (P. wasabiae) using restriction enzyme analysis. All isolates were also checked for phenotypic properties and pathogenicity on potato tubers. Phylogenetic analysis, using clustering methods on partial sequences of the 16S rDNA, showed that all Iranian isolates grouped with the Pcc type strain (ATCC1571) except for six isolates from Kashmar. Based on rep-PCR cluster analysis of the pairwise similarity values using UPGMA, bacterial isolates could be separated into three clusters, the third of which (Kashmar isolates) was allocated in a distinct group. The similarity coefficients among strains ranged from 0.2 to 1. Rep-PCR primers disclosed different levels of genetic heterogeneity among Pcc isolates. No clear association was found between clustering based on rep-PCR and the geographical origin of the isolates, original host plant cultivars and the year of collection. Source

Taghdareh G.,Ferdowsi University of Mashhad | Baghaee-Ravari S.,Ferdowsi University of Mashhad | Moslemkhani C.,Seed and Plant Certification and Registration Institute | Mahdikhani-Moghaddam E.,Ferdowsi University of Mashhad
European Journal of Plant Pathology

The plasmid content of 38 Iranian Erwinia amylovora strains isolated from five different plant species and geographical locations from 2005 to 2013 were evaluated. With exception to the pEA29 plasmid, isolates obtained from quince and hawthorns carry the pEU72 plasmid. RFLP analysis of PstI amplified fragment of plasmid pEA29 using HpaII revealed three known fragments in all strains. Based on the observed polymorphism in the larger one that varied between 360 to 400 bp, all Iranian strains clustered into four groups. In order to determine the exact number of short-sequence DNA repeats, 18 strains were chosen for PstI fragment sequencing. About 63 % of the strains carried a repeat number of 4. The remaining 37 % harboured five, seven and eight repeats. Sometimes a correlation was seen between the RFLP patterns and SSR grouping with respect to other factors including the host plant species, geographical region or sampling year. This study reported the genetic variation of E. amylovora strains for the first time in Iran, based on enzymatic digestion of the PstI fragment of pEA29 and evaluation of the short sequence repeat units. It seems that, the latter method may be reliable for E. amylovora strains grouping. © 2014, Koninklijke Nederlandse Planteziektenkundige Vereniging. Source

Forghani S.H.,Seed and Plant Certification and Registration Institute | Marouf A.,Iranian Research Institute of Plant Protection
Biharean Biologist

It has been long reported that storage pests inflict great deal of damages on grain yields across the world. Stores in the central provinces of Iran are specially dominated with pest population. To address the problem, a research project was conducted to identify the main pests of wheat and barley grains in eight provinces during 2011. Different samples were collected from 20 stores, later on were incubated under lab conditions (25±1)°C; (16L:8D) and RH (35±5) %) and finally different species of insects were identified and recorded as followings: Sitophilus granarius (Linné, 1758), Sitophilus oryzae (L), Tribolium custaneum (Herbst, 1797), Trogoderma granarium Everts, Trogoderma variabile Ballion, Tenebrio sp., Dermestes sp.. Comparing the population figures Sitophilus oryzae indentified as the most abundant pest species with in this region. In addition, for the first time in Iran, Trogoderma variabile was indentified and reported in this study. The findings could be applied as the first step at grain stores in decision making for integrated pest management in these areas. © Biharean Biologist, Oradea, Romania, 2015. Source

Tahernezhad Z.,Seed and Plant Certification and Registration Institute | Musavi Z.,University of Zabol | Zamani M.J.,Iranian Seed and Plant Improvement Institute | Jafar Aghaei M.,National Plant Gene Bank of Iran | Rostam Foroudi B.,Iranian Seed and Plant Improvement Institute
Genetic Resources and Crop Evolution

Variation of high molecular weight glutenin subunits (HMW-GS) in 28 Iranian Aegilops tauschii (2n = 2x = 14, DD) accessions studied by sodium dodecyl sulphate electrophoresis method (SDS-PAGE). The results showed high variation of HMW-GS in the accessions. The range of frequency in 14 HMW-GS combinations was 3. 57-25 % in the accessions. AMOVA showed the molecular variance between the geographic areas was lower than within the geographic areas. According to Nei's genetic diversity, the highest diversity levels were in Semnan, Golestan and Azarbayjan, on the other hand the lowest levels of diversity were found in Khorasan, Gilan and Mazandaran accessions. Hence, the Caspian Sea South East accessions also Azerbayjan in Iran have more diversity. AMOVA did not show variance between strangulata and tauschii but there was more genetic diversity in ssp. tauschii subspecies in comparison of ssp. strangulata according to Nei's gene diversity and Shannon information index. It showed Iranian Ae. tauschii have a good potential for bread making quality improvement in bread wheat. © 2012 Springer Science+Business Media Dordrecht. Source

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