Wiik-Nielsen C.R.,Section of Food Bacteriology and GMO |
Wiik-Nielsen C.R.,National Veterinary Institute |
Holst-Jensen A.,Section of Food Bacteriology and GMO |
Boydler C.,Section of Food Bacteriology and GMO |
Berdal K.G.,Section of Food Bacteriology and GMO
Aquaculture Nutrition | Year: 2011
The impact of dietary DNA on metabolism and health of animals and humans has received little attention, except in the context of genetically modified organisms (GMOs) and horizontal gene transfer. In a series of studies, we have investigated the uptake and persistence of dietary DNA in Atlantic salmon (Salmo salar L.). The objective of this study was to investigate the uptake and persistence of dietary DNA of soybean and maize origin. A feeding experiment on salmon was started at late yolk sac stage and lasted for 7months. The fish were randomly distributed in groups in indoor tanks and fed different types of feed. After the last feeding, the fish were starved for 24h before samples were dissected. Using the polymerase chain reaction (PCR) amplification of short targets from the chloroplast ribulose-1,5-carboxylase large subunit (rbcL) gene present in some of the feed components, the uptake and transport of dietary DNA from plant ingredients to tissues could be studied. The dietary DNA, of plant origin, was found to be present in all tissues investigated and their concentrations were determined. © 2010 Blackwell Publishing Ltd.