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Sanchez-Casas R.M.,Autonomous University of Nuevo Leon | Alpuche-Delgado R.H.,Secretaria de Salud Del Estado de Quintana Roo. Cancun | Blitvich B.J.,Iowa State University | Diaz-Gonzalez E.E.,Autonomous University of Nuevo Leon | And 11 more authors.
Southwestern Entomologist | Year: 2013

In October 2011, the State Health Department announced that several laboratory-confirmed cases of dengue had occurred among residents in two neighborhoods of Benito Juarez, Quintana Roo State, Mexico To identify the dengue virus serotype(s) temporally and spatially associated with the cases, entomologic-based virus surveillance was initiated in October 2011 in both neighborhoods Adult mosquitoes were collected from 88 houses by CDC-backpack aspirator, and all female Aedes aegypti L (n = 419) were individually homogenized and assayed in pools of as many as 10 by reverse transcriptionpolymerase chain reaction (RT-PCR) using dengue virus-specific primers Five (12%) of 41 pools were positive for dengue virus RNA The individual mosquitoes that comprised the pools were analyzed separately by RT-PCR using dengue virus serotype-specific primers Six mosquitoes were positive for dengue virus serotype-2 (DENV-2) RNA, three of which were collected in the same house The mean number of female Ae aegypti collected in each house was 4.76 ± 6.19 The overall dengue virus-infection rate in female Ae aegypti was 1.4% Interestingly, most (60%) of mosquito females were collected only from 15 (17%) houses In summary, we provide evidence of recent DENV-2 transmission in Quintana Roo State. Source


Mendez-Galvan J.,Autonomous University of Nuevo Leon | Sanchez-Casas R.M.,Autonomous University of Nuevo Leon | Gaitan-Burns A.,Autonomous University of Nuevo Leon | Diaz-Gonzalez E.E.,Autonomous University of Nuevo Leon | And 6 more authors.
Southwestern Entomologist | Year: 2014

Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were grouped into 220 to use semi-nested RT-PCR for DENV, and positive groups were analyzed individually. Five groups (2.27%) were positive for DENV. Individual analysis yielded eight groups that tested positive, six with DENV-2, one DENV-1, and one DENV-4. The latter was not reported by the surveillance system that year. The mean number of female mosquitoes per household was 3.77 ± 5.71, and the rate of viral infection of Ae. aegypti was 0.4%. Most infected mosquitoes (49%) were concentrated in 10% of the houses. Monitoring Ae. aegypti infected with DENV has the potential to complement the current system of clinical and entomological surveillance. Source

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