The use of ISSR markers for species determination and a genetic study of the invasive lionfish in Guanahacabibes, Cuba [Uso de marcadores ISSR para la determinación de especies y estudios genéticos del pez león, especie invasora en Guanahacabibes, Cuba]
Labastida E.,Colegio de Mexico |
Henaut Y.,Conducta |
del Carmen Garcia-Rivas M.,Secretaria de Medio Ambiente y Recursos Naturales |
Chevalier P.P.,Acuario Nacional de Cuba |
MacHkour-M'rabet S.,Colegio de Mexico
Latin American Journal of Aquatic Research | Year: 2015
The red lionfish (Pterois volitans) and devil fire-fish (Pterois miles) are invasive species that pose a threat to the biodiversity and stability of coral reefs in the Western Atlantic, Gulf of Mexico and Caribbean Sea. Species identification of lionfish is uncertain in some parts of Cuba, and research has mainly been focused on their biology and ecology. The principal aim of this study was to determine highly polymorphic markers (Inter Simple Sequence Repeat, ISSR) that could be used in research on lionfish population genetics in addition to confirming the presence of Pterois species in the Guanahacabibes National Park. The genetic profile or “fingerprint” of individuals collected in Mexico, formally identified as P. volitans, was compared with the genetic profile of specimens from Cuba. There were very few “diagnostic bands” and a high number of “common bands”, demonstrating that the same species exists in both countries. Furthermore, Nei's genetic distance and the unrooted tree do not show significant differences between both localities. In light of these results, we can confirm the presence of P. volitans in the Guanahacabibes National Park, Cuba. This study demonstrates the functionality of ISSR as a molecular tool for species identification and their application for genetic population studies of this invasive fish species. © 2015, Escuela de Ciencias del Mar. All rights reserved.
Lacy R.,Metropolitan Autonomous University |
Molina M.,University of California at San Diego |
Molina M.,Centro Mario Molina para Estudios Estrategicos sobre Energia y Medio Ambiente |
Vaca M.,Metropolitan Autonomous University |
And 6 more authors.
International Journal of Greenhouse Gas Control | Year: 2015
Carbon capture, use and geological storage (CCUS) could be feasible when fossil fuel-power plants are close to oil and gas reservoirs where CO2-enhanced oil recovery (EOR) technologies are applicable. In this research, we used a novel "well-to-well" approach that included the operations from natural gas production at oil field to CO2 injection for EOR operations at depleted oil fields. This paper includes estimates for greenhouse gas (GHG) emissions arising from a hypothetical CCUS case with a natural gas combined cycle power plant (NGCC) and Global Warming Potential (GWP) impact, by using Life-Cycle Assessment (LCA) methodology. Our research comprises a comparison with other electricity-generation technologies, including super critical pulverized carbon (SCPC), NGCC without CO2 capture, geothermal, mini-hydro, wind and nuclear. The LCA stages that were undertaken in this study were natural gas supply system, electricity generation, CO2 capture, CO2 transport, EOR operations and environmental monitoring. Three different functional units were used in this study: MJ, kWh and produced oil barrel (bbl). Results indicate that the energy produced by the described CCUS system had an environmental impact on climate change (global warming potential) of 0.044kg CO2e/MJ. The NGCC power plant with carbon capture unit would produce 0.177kg CO2e/kWh, representing about 21% and 36% of the estimated values for the SCPC and NGCC (without CCS) cases respectively, and about 24% less greenhouse gas emissions than the geothermal scenario. The oil produced in the EOR stage had a greenhouse gas emission rate of 38kg CO2e per oil barrel and 251kg CO2e per oil barrel in the whole CCUS system. © 2015 Elsevier Ltd.