Second Workers Hospital of Liaohe Oil Field

Panjin, China

Second Workers Hospital of Liaohe Oil Field

Panjin, China
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Tang X.,Central Hospital of Liaohe Oil Field | Wang Y.,Central Hospital of Liaohe Oil Field | Yi H.-B.,Second Workers Hospital of Liaohe Oil Field | Pang T.-S.,Central Hospital of Liaohe Oil Field
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Intramyocardial transplantation of autologous umbilical cord-derived mesenchymal stem cells for repair of myocardial tissue damage is paid increasing attention in the cardiovascular field. OBJECTIVE: Human umbilical cord mesenchymal stem cells were isolated and cultured. Passage 2 human umbilical cord mesenchymal stem cells were treated with various concentratins of 5-azacytidine (2.5, 5, 10, 20, 40, 80 μmol/L) for 24 hours. After removal of 5-azacytidine, cells were cultured for another 4 weeks. RESULTS AND CONCLUSION: Before 5-azacytidine treatment, filament-like structures or particles were not observed in the cells, but the amount of cytoplasm was less and uniform, nuclear/cytoplasm ratio was high, cells exhibited typical fusiform appearance and grew in a swirl-like manner, and nucleolus was obvious. After treatment with 5-azacytidine for 24 hours, some cells died in each group, and typical fusiform appearance turned into stick-like or column-like appearance, especially in the 40 and 80 μmol/L 5-azacytidine groups. Reverse transcription-PCR results showed that atrial natriuretic peptide and α-skeletal actin gene expression levels were detected on human umbilical cord mesenchymal stem cells after treatment with 2.5 or 40 μmol/L 5-azacytidine for 4 weeks or with 5, 10, 20 μmol/L 5-azacytidine for 1, 2, 3 and 4 weeks. These findings suggest that 5-azacytidine-induced human umbilical cord mesenchymal stem cells express the specific gene of myocardiocytes.


Tang X.,Central Hospital of Liaohe Oil Field | Wang N.-N.,Central Hospital of Liaohe Oil Field | Yi H.-B.,Second Workers Hospital of Liaohe Oil Field | Wang Y.,Central Hospital of Liaohe Oil Field | Pang T.-S.,Central Hospital of Liaohe Oil Field
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Human umbilical cord mesenchymal stem cells possess multi-differentiation potential. There are few studies describing whether human umbilical cord mesenchymal stem cells can be induced to differentiate into cardiomyocytes. Objective: To investigate cardiac troponin I expression in human umbilical cord mesenchymal stem cells after 5-azacytidine induction in vitro. Methods: Passage 2 human umbilical cord mesenchymal stem cells were digested, centrifuged and deposited. Cell suspension was prepared and cell concentration was adjusted to be 2×107/L. Then cells were cultured for 24 hours after addition of various concentrations of 5-azacytidine (80, 40, 20, 10, 5, 2.5, 0 μmol/L). After removal of 5-azacytidine, cells were cultured for another 4 weeks. Results and Conclusion: After treatment with 5-azacytidine for 24 hours, some cells died in each group, and typical fusiform appearance turned into stick-like or column-like appearance, especially in the 40 and 80 μmol/L 5-azacytidine groups. Immunohistochemical staining results showed that cardiac troponin I expression was detected in human umbilical cord mesenchymal stem cells in 5, 10, 20, 40, 80 μmol/L 5-azacytidine groups after induction for 2 weeks, but it was not detected in the control and 2.5 μmol/L 5-azacytidine groups. Five high-fold fields were selected for cell counting. The number of cardiomyocytes was not significantly different between 80, 40, 20, 10 μmol/L 5-azacytidine groups, but it was significantly higher compared to that in the 5 μmol/L 5-azacytidine group. After 5-azacytidine treatment for 4 weeks, typical sarcomeres and cardiac atrium particles were observed in some human umbilical cord mesenchymal stem cells. Such changes were not observed in the control group in which 5-azacytidine induction was not used. These findings suggest that human umbilical cord mesenchymal stem cells were induced to cardiomyocyte-like cells by 5-azacytidine.


Tang X.,The Central Hospital of Liaohe Oil Field | Wang Y.,The Central Hospital of Liaohe Oil Field | Yi H.-b.,Second Workers Hospital of Liaohe Oil Field | Pang T.-s.,The Central Hospital of Liaohe Oil Field
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Studies addressing the induced effect of different-concentration 5-azacytidine on the in vitro differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into cardiomyocytes are fewer. OBJECTIVE: To select a suitable concentration of 5-azacytidine to induce differentiatiown of hUC-MSCs into cardiomyocytes in vitro. METHODS: Second passage hUC-MSCs were cultured in culture medium containing different -concentration 5-azacytidine (2.5, 5, 10, 20, 40, 80 μmol/L, respectively). After incubating for 24 hours, the medium was changed to complete medium without 5-azacytidine. Thereafter, the cells were cultivated for 4 weeks. Two weeks after the cells were treated with 5-azacytidine, cardiac troponin I was assayed by immunohistochemical methods and the ratio of positive cells for cardiac troponin I was counted. RESULTS AND CONCLUSION: Seven days after hUC-MSCs were treated with 5-azacytidine, the cellular morphology began to change. The cells arranged closely and in parallel. The cellular morphology of 40 and 80 μmol/L groups changed obviously. At 4 weeks, the refraction and activity of cultured cells declined in the 40 and 80 μmol/L groups, and some of the cells died. At 2 weeks, cardiac troponin I was positive for hUC-MSCs in 5, 10, 20, 40 and 80 μmol/L groups and negative in 2.5 μmol/L and control groups. The ratio of positive cells for cardiac troponin I in 10, 20, 40 and 80 μmol/L groups was significantly higher than that of 5 μmol/L group (P < 0.05). l0 μmol/L 5-azacytidine is the optimal concentration for hUC-MSCs differentiation into cardiomyoctyes.


Liang J.W.,Liaoning Cancer Hospital and Institute | Zheng Z.C.,Liaoning Cancer Hospital and Institute | Yu T.,Liaoning Cancer Hospital and Institute | Wang X.,Second Workers Hospital of Liaohe Oil Field | Zhang J.J.,Liaoning Cancer Hospital and Institute
European Journal of Surgical Oncology | Year: 2014

Backgroud Adjuvant chemoradiotherapy (CRT) for patients with gastric cancer after D2 lymphadenectomy remains controversial. The objective of the present meta-analysis was to analyze efficacy and safety of postoperative CRT and establish a consensus on whether it is suitable for the patients. Methods We searched PubMed, Ovid, Cochrane, and Web of Science. Statistical analysis was carried out by STATA version 12.0 software. The quality of evidence was assessed by Jadad and the Newcastle-Ottawa quality assessment scale. Results Six studies involving 2135 patients were included for the meta-analysis. The results showed that, compared with non-CRT, postoperative adjuvant CRT was associated with a significant improvement in 5-year overall survival (OS) (HR = 0.79, 95% CI 0.68-0.92, P = 0.002) and 5-year relapse-free survival (RFS) (HR = 0.81, 95% CI 0.70-0.93, P = 0.004). However, there were no differences in distant metastasis (RR = 0.93, 95% CI 0.82-1.06, P = 0.304) and treatment-related toxicity between the two groups. Conclusions From the results of our study, postoperative adjuvant CRT may be associated with longer 5-year OS and 5-year RFS in patients with D2 lymphadenectomy, but might not improve 5-year disease-free survival compared to non-CRT. Methodologically high-quality comparative studies are needed for further evaluation. © 2014 Elsevier Ltd.

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