Zhou J.-C.,Second Peoples Hospital of Zhanjiang |
Zheng L.,Second Peoples Hospital of Zhanjiang |
Liu J.-H.,Second Peoples Hospital of Zhanjiang |
Li Y.,Second Peoples Hospital of Zhanjiang |
And 2 more authors.
Journal of Leukemia and Lymphoma | Year: 2013
Objective To explore the inhibitory effect of anti-miRNA-17 oligonucleotide on leukemic K562 cells. Methods K562 cells were transfected with anti-miRNA-17 oligonucleotide, cell viability was analyzed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zoliunbromide (MTT) assay. Apoptosis was detected by flow cytometry, expression of miRNA-17 in K562 cells was measured by real-time PCR. Results MTT results showed transfection of antisense nucleic acid significantly decreased cell proliferation activity, after 24, 48, 72 h they were respectively 0.8719 ±0.001, 0.7102 ±0.002, 0.5507 ±0.001, the difference being statistically significant (P < 0.05) when compared to the random control (t = 182.575, 269.77, 660.4) or control group (t = 537.98, 571.20, 1230.51). FCM test results showed that after 48 h of transfecting antisense nucleic acid apoptosis rate was (20.14 ± 0.01) %, and was statistically significant compared to the randomized control or blank control group (t = 2347.6, 2568.2, P < 0.01). Fluorescence real-time quantitative PCR confirmed antisense nucleic acid significantly decreased the relative expression level of miR-17 in K562 cells (0U07)U The difference was statistically significant compared to the random group or blank group (1, 1.01) (t = 148.63, 147.04, P < 0.05). Conclusion Targeted inhibition of miR-17 with oligonucleotide can suppress K562 cell growth and induce apoptosis.