Guan J.-W.,University of Sichuan |
Guan J.-W.,Second Peoples Hospital of Zhanjiang |
Huang C.-Q.,University of Sichuan |
Huang C.-Q.,Third Hospital of Mianyang |
And 6 more authors.
Journal of Alzheimer's Disease | Year: 2011
This study examined the association between hypertension and AD by using a quantitative meta-analysis of longitudinal studies. EMBASE and MEDLINE were searched for articles published up to February 2011. All studies that examined the association of hypertension or antihypertensive medication use with the onset of AD were included. Pooled relative risks (RR) were calculated using fixed and random effects models. Twelve studies met our inclusion criteria for this meta-analysis. All subjects were without dementia at baseline. Among them, 9 studies compared the incidence of AD between subjects with (7,270) and without (8,022) hypertension. The quantitative meta-analysis showed that there was no significant difference in incidence of AD (RR: 1.02, 95% confidence interval (CI): 0.91-1.14) between subjects with and without hypertension. Seven studies compared the incidence of AD between subjects with (8,703) and without (13,041) antihypertensive medication use. The quantitative meta-analysis showed that there was no significant difference in incidence of AD (RR: 0.90, 95% CI: 0.79-1.03) between subjects with and without antihypertensive medication use. The quantitative meta-analysis showed that neither hypertension nor antihypertensive medication use was associated with risk for incident AD. © 2011-IOS Press and the authors. All rights reserved.
Zhou J.-C.,Second Peoples Hospital of Zhanjiang |
Zheng L.,Second Peoples Hospital of Zhanjiang |
Liu J.-H.,Second Peoples Hospital of Zhanjiang |
Li Y.,Second Peoples Hospital of Zhanjiang |
And 2 more authors.
Journal of Leukemia and Lymphoma | Year: 2013
Objective To explore the inhibitory effect of anti-miRNA-17 oligonucleotide on leukemic K562 cells. Methods K562 cells were transfected with anti-miRNA-17 oligonucleotide, cell viability was analyzed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zoliunbromide (MTT) assay. Apoptosis was detected by flow cytometry, expression of miRNA-17 in K562 cells was measured by real-time PCR. Results MTT results showed transfection of antisense nucleic acid significantly decreased cell proliferation activity, after 24, 48, 72 h they were respectively 0.8719 ±0.001, 0.7102 ±0.002, 0.5507 ±0.001, the difference being statistically significant (P < 0.05) when compared to the random control (t = 182.575, 269.77, 660.4) or control group (t = 537.98, 571.20, 1230.51). FCM test results showed that after 48 h of transfecting antisense nucleic acid apoptosis rate was (20.14 ± 0.01) %, and was statistically significant compared to the randomized control or blank control group (t = 2347.6, 2568.2, P < 0.01). Fluorescence real-time quantitative PCR confirmed antisense nucleic acid significantly decreased the relative expression level of miR-17 in K562 cells (0U07)U The difference was statistically significant compared to the random group or blank group (1, 1.01) (t = 148.63, 147.04, P < 0.05). Conclusion Targeted inhibition of miR-17 with oligonucleotide can suppress K562 cell growth and induce apoptosis.