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Miao Y.-L.,Kunming Medical University | Xiao Y.-L.,The second peoples hospital of Yunnan province | Du Y.,Kunming Medical University | Duan L.-P.,Kunming Medical University
World Journal of Gastroenterology | Year: 2013

AIM: To identify peripheral blood mononuclear cell (PBMC) gene expression profiles of ulcerative colitis (UC) patients, using oligonucleotide microarrays, to gain insights into UC molecular mechanisms. METHODS: The Human OneArray microarrays were used for a complete genome-wide transcript profiling of PBMCs from 12 UC patients and 6 controls. Differential analysis per gene was performed with a random variance model; t test and P values were adjusted to control the false discovery rate (5%). Gene ontology (GO) was deployed to analyze differentially expressed genes at significant levels between patients and controls to identify the biological processes involved in UC. RESULTS: Comparative analysis revealed that 4438 probes (4188 genes) were differentially expressed between the two groups, of which 3689 probes (3590 genes) were down-regulated whereas 749 probes (598 genes) were up-regulated. Many disregulated genes in our data have been reported by previous microarray studies carried out on intestinal mucosa samples, such as S100A8, CEACAM1 and S100A9. GO enrichment analysis revealed 67 high enrichment up-regulated categories and one significant down-regulated category. The up-regulated genes were mainly involved in immune and inflammatory response, cell cycle and proliferation, DNA metabolism and repair. CONCLUSION: Gene expression profiling of PBMCs from patients with UC has highlighted several novel gene categories that could contribute to the pathogenesis of UC. © 2013 Baishideng. All rights reserved. Source


Zhang H.,The second peoples hospital of Yunnan province
International Journal of Ophthalmology | Year: 2011

AIM: To estimate the therapeutic effect and safety of small incision phacoemulsification and IOL implantation on uveitis coexisting with cataract. METHODS: Phacoemulsification with small incision and IOL implantation were performed in 40 eyes of 32 patients through a small corneal incision, and the small pupil and posterior synechia were carefully handled, then the postoperative visual acuity and complications were retrospectively analyzed. RESULTS: Follow-up 1-13 months (7.6 months in average), all cases were achieved round pupil and visual acuity was improved. Posterior capsule ruptured was in 3 eyes and tridemia occurred in 2 eyes during surgery. Corneal edema, fibrous exudates in anterior chamber, posterior synechia and uveitis recurrence were the main postoperative complications. CONCLUSION: This method with a small corneal incision can protect iris from injury. It is a safe and effective method for management of uveitis coexisting with cataract, there is little inflammatory reaction, and visual acuity is satisfactory. Source


Zhou Y.-H.,CAS Kunming Institute of Zoology | Zhou Y.-H.,University of Chinese Academy of Sciences | Liu F.-L.,CAS Kunming Institute of Zoology | Liu F.-L.,University of Chinese Academy of Sciences | And 6 more authors.
PLoS ONE | Year: 2011

Background: Co-infection with HIV and HCV and/or HBV is highly prevalent in intravenous drug users (IDUs). Because of the proximity to the "Golden Triangle", HIV prevalence among the IDUs is very high in the China-Myanmar border region. However, there are few studies about co-infection with HIV and HCV and/or HBV, especially in the region that belongs to Myanmar. Methods: 721 IDUs, including 403 Chinese and 318 Burmese, were investigated for their HIV, hepatitis B virus (HBV), and hepatitis C virus (HCV) serological status. Statistical analysis was performed to evaluate the differences of the epidemic situation between the Chinese IDUs and the Burmese IDUs. Results: Among the Chinese IDUs and the Burmese IDUs, HCV infection was the most prevalent (69.0% vs 48.1%, P<0.001), followed by HBV (51.6% vs 43.1%, P<0.05) and HIV (33.7% vs 27.0%, P>0.05). Besides, there were more HIV-HBV co-infected IDUs (20.1% vs 11.3%, P<0.005), and HIV-HCV co-infected IDUs (31.8% vs 23.9%, P<0.05) in China than in Myanmar, as well as HIV-HBV-HCV triple infection (19.1% vs 10.4%, P<0.005). Conclusion: Co-infection with HIV and HCV and/or HBV is highly prevalent among the IDUs in the China-Myanmar border region. The HIV epidemic appears to be in a downward trend, compared with previous reports. However, all infections were more prevalent among the Chinese IDUs than among the Burmese. © 2011 Zhou et al. Source


Chen X.-Z.,The second peoples hospital of Yunnan province | Fang Y.,Kunming Medical University | Ling B.,The second peoples hospital of Yunnan province
Mitochondrial DNA | Year: 2014

To characterize the somatic mutation spectrum of mitochondrial DNA at D310 in Chinese lung cancer patients and evaluate its potential significance in Chinese lung cancer diagnosis, in this study, 237 samples, including lung tumor, adjacent normal tissue and blood samples of 79 lung cancer patients were analyzed. By comparing sequences of D310 between lung cancer tissues, adjacent normal tissue and blood samples, the somatic mutations at D310 were detected in 17.72% (14/79) of Chinese lung cancer patients; this implied that somatic mutations at D310 could be served as valuable biomarker for diagnostic of Chinese lung cancer. Further analyses indicated that deletion and heterogeneity were the predominant characters for somatic mutations detected at D310 of Chinese lung cancer patients. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted Source


Xu J.,The second peoples hospital of Yunnan province | Yin J.,The second peoples hospital of Yunnan province
International Journal of Ophthalmology | Year: 2011

AIM: To discuss the clinical effectiveness of superior rectus and inferior rectus muscle Hummelscheim displacement in treating abducent paralytic strabismus. METHODS: Totally 20 cases (21 eyes) of severe esotropia caused by abducent paralysis were treated by superior rectus and inferior rectus muscle Hummelscheim displacement during January 2004 to June 2010 in our hospital. The effectiveness was analyzed. RESULTS: Treated by superior rectus and inferior rectus muscle Hummelscheim displacement, esotropia of 20 cases of abducent paralytic strabismus was corrected significantly. After 6 months to 3 years follow-up, the eye position kept stable and no obvious change occurred. CONCLUSION: Superior rectus and inferior rectus muscle Hummelscheim displacement is practical in treating abducent paralytic strabismus and its outcome is stable. Source

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