Fang J.,Jiangsu University |
Wang H.,Second Peoples Hospital of Wuxi |
Wang H.,Nanjing Medical University |
Liu Y.,Jiangsu University |
And 3 more authors.
Cancer Science | Year: 2017
Keratin8 (KRT8) is the major component of the intermediate filament cytoskeleton and predominantly expressed in simple epithelial tissues. Aberrant expression of KRT8 is associated with multiple tumor progression and metastasis. However, the role of KRT8 in gastric cancer (GC) remains unclear. In this study, KRT8 expression was investigated and it was found to be upregulated along with human GC progression and metastasis at both mRNA and protein levels in human gastric cancer tissues. In addition, KRT8 overexpression enhanced the proliferation and migration of human gastric cancer cells, whereas the knock-down of KRT8 by siRNA only inhibited migration of human gastric cancer cells. Integrinβ1-FAK-induced epithelial-mesenchymal-transition (EMT) only existed in the high KRT8 cells. Furthermore, KRT8 overexpression led to increase in p-smad2/3 levels and TGFβ dependent signaling events. KRT8 expression in GC was related to tumor clinical stage and worse survival. Kaplan–Meier analysis proved that KRT8 was associated with overall survival of patients with GC that patients with high KRT8 expression tend to have unfavorable outcome. Moreover, Cox's proportional hazards analysis showed that high KRT8 expression was a prognostic marker of poor outcome. These results provided that KRT8 expression may therefore be a biomarker or potential therapeutic target to identify patients with worse survival. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
Hu Z.,General Hospital of Jinan Military Command Region |
Sun Y.,Shanghai University |
Wang Q.,Shanghai University |
Han Z.,Second Peoples Hospital of Wuxi |
And 6 more authors.
Clinical Chemistry and Laboratory Medicine | Year: 2013
Background: Red blood cell distribution width (RDW) is increased in liver disease. Its clinical significance, however, remains largely unknown. The aim of this study was to identify whether RDW was a prognostic index for liver disease. Methods: We studied, retrospectively, 33 patients with non-cirrhotic HBV chronic hepatitis, 125 patients with liver cirrhosis after HBV infection, 81 newly diagnosed primary hepatocellular carcinoma (pHCC) patients, 17 alcoholic liver cirrhosis patients and 42 patients with primary biliary cirrhosis (PBC). Sixty-six healthy individuals represented the control cohort. We analyzed the relationship between RDW on admission and clinical features. The association between RDW and hospitalization outcome was estimated by receiver operating curve (ROC) analysis and a multivariable logistic regression model. Results: Increased RDW was observed in liver disease patients. RDW was positively correlated with serum bilirubin and creatinine levels, prothrombin time, and negatively correlated with platelet counts and serum albumin concentration. A subgroup analysis, considering the different etiologies, revealed similar findings. Among the patients with liver cirrhosis, RDW increased with worsening of Child-Pugh grade. In patients with PBC, RDW positively correlated with Mayo risk score. Increased RDW was associated with worse hospital outcome, as shown by the AUC [95% confidence interval (CI)] of 0.76 (0.67-0.84). RDW above 15.15% was independently associated with poor hospital outcome after adjustment for serum bilirubin, platelet count, prothrombin time, albumin and age, with the odds ratio (95% CI) of 13.29 (1.67-105.68). Conclusions: RDW is a potential prognostic index for liver disease. © 2013 by Walter de Gruyter Berlin Boston 2013.
Zhou C.,Jiangsu Taizhou Peoples Hospital |
Zhang S.,First Peoples Hospital of Suqian |
Gong Q.,Jiangsu Taizhou Peoples Hospital |
Hao A.,Second Peoples Hospital of Wuxi
Virology Journal | Year: 2015
Background: Recently, a diverse group of viruses with circular, replication initiator protein(Rep) encoding, single stranded DNA (CRESS-DNA) genomes, were discovered from wide range of eukaryotic organisms ranging from mammals to fungi. Gemycircularvirus belongs to a distinct group of CRESS-DNA genomes and is classified under the genus name of Gemycircularvirus. Findings: Here, a novel gemycircularvirus named GeTz1 from cerebrospinal fluid sample of a child with unexplainable encephalitis was characterized. The novel gemycircularvirus encodes two major proteins, including a capsid protein (Cap) and a replication-associated protein (Rep). Phylogenetic analysis based on the amino acid sequence of Rep indicated that GeTz1 clusters with one gemycircularvirus discovered from bird (KF371633), sharing 46.6 % amino acid sequence identity with each other. Conclusion: A novel gemycircularvirus was discovered from cerebrospinal fluid sample of a child with unexplainable encephalitis. Further studies, such as testing human sera for specific antibodies, should be performed to investigate whether gemycircularvirus infects human and is associated with encephalitis. © 2015 Zhou et al.
Cheng X.,Nantong University |
Gan L.,Second Peoples Hospital of Wuxi |
Zhao J.,Nantong University |
Chen M.,Nantong University |
And 2 more authors.
Neurochemical Research | Year: 2013
Ataxin-10 is a cytoplasmic protein that belongs to the family of armadillo repeat proteins and the ataxin proteins are ubiquitously expressed in nervous tissue. A loss of Ataxin-10 in primary neuronal cells causes increased apoptosis of cerebellar neurons. Knockdown of ATXN10 with siRNA in HeLa cells results in cytokinesis defects-multinucleation. Because of the essential role of Ataxin-10 in nervous system and cellular cytokinesis, we investigated the spatiotemporal expression of Ataxin-10 in a rat sciatic nerve crush (SNC) model. After never injury, we observed that Ataxin-10 had a significant up-regulation from 3d, peaked at day 5 and then gradually decreased to the normal level at 4 weeks. At its peak expression, Ataxin-10 expressed mainly in Schwann cells and macrophages of the distal sciatic nerve segment from injury, but had few co-localizations in axons. Besides, the peak expression of Ataxin-10 was in parallel with proliferating cell nuclear antigen (PCNA), and Ataxin-10 co-labeled with PCNA. Thus, all of our findings suggested that Ataxin-10 may be involved in the pathophysiology of sciatic nerve after SNC. © 2013 Springer Science+Business Media New York.
Ji X.,Second Peoples Hospital of Wuxi |
Li J.,Nanjing Medical University |
Yang C.,Second Peoples Hospital of Wuxi
Journal of Immunoassay and Immunochemistry | Year: 2015
Herein, a simple and novel electrochemical method for the detection of potassium ions (K+) was developed. In the presence of potassium ions, the potassium ions aptamer will form a G-quadruplex complex. Thus, further addition of hemin in the presence of potassium ions will lead to the formation of a recombined G-quadruplex. Then the electroactive label, hemin, will give an electrochemical response. The linear range of the method covered a large variation of K+ concentration from 0.1 nM to 0.1 M and the detection limit of 0.1 nM was obtained. Moreover, this assay was able to detect K+ with high selectivity and had great potential applications. © 2015 Taylor & Francis Group, LLC.
Ren T.-L.,Second Peoples Hospital of Wuxi |
Yang C.-J.,Second Peoples Hospital of Wuxi |
Fang D.-Y.,Second Peoples Hospital of Wuxi |
Shi G.-X.,Second Peoples Hospital of Wuxi |
Zhu X.,Jiangsu Institute of Nuclear Medicine
Acta Crystallographica Section E: Structure Reports Online | Year: 2012
The asymmetric unit of the title ion-pair complex, (C9H 12NO2)2[Ni(C4N2S 2)2], contains two 1-(eth-oxy-carbonyl-meth-yl)pyridinium cations and one bis-(1,2-dicyano-ethene-1,2-dithiol-ato)nickelate(II) dianion, which exhibits a slightly distorted square-planar coordination geometry. In the crystal, the cations are linked by strong C - H⋯O hydrogen bonds into C(6) chains along . The cations and anions are linked into a three-dimensional architecture by weak C - H⋯N and C - H⋯S interactions.
Zhang K.,Jiangsu Institute of Nuclear Medicine |
Ren T.,Second Peoples Hospital of Wuxi |
Wang K.,Jiangsu Institute of Nuclear Medicine |
Zhu X.,Jiangsu Institute of Nuclear Medicine |
And 2 more authors.
Chemical Communications | Year: 2014
A new Exo III assisted strand-cleavage cycle and ligand-responsive quadruplex formation strategy for amplified and label-free detection of IFN-γ was reported with a detection limit of 0.1 pM and a visual detection limit of 20 pM by the naked eye. © the Partner Organisations 2014.
Han S.,Second Peoples Hospital of Wuxi |
Lv L.,Second Peoples Hospital of Wuxi |
Wang X.,Second Peoples Hospital of Wuxi |
Zhao H.,Second Peoples Hospital of Wuxi
International Journal of Clinical and Experimental Pathology | Year: 2016
Objectives: This study aimed to explore the effect of AXIN2 and MMP7 polymorphisms on non-small cell lung cancer (NSCLC) susceptibility; in addition, the interaction between gene polymorphisms and environment was also displayed. Methods: The genotyping was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 102 patients with NSCLC and 120 healthy controls. Odds ratio (OR) and 95% confidence interval (CI) were calculated to assess the relevance strength of AXIN2 and MMP7 polymorphisms with NSCLC. The χ2 test was used to compare to the frequencies difference of genotypes and alleles in cases and controls and Hardy-Weinberg equilibrium (HWE) test. The haplotype and interaction analyses were performed by haploview and MDR software, respectively. Results: The genotype frequencies of all polymorphisms in the control group conformed to HWE. GG genotype frequency of AXIN2 rs2240307 polymorphism was significantly higher in cases than controls (P=0.041). Similarly, rs2240308 in AXIN2 gene was also increased the susceptibility to NSCLC remarkably (OR=2.412, 95% CI=1.025-5.674). What's more, haplotype A-G-G in AXIN2 might play a protective role in NSCLC (OR=0.462, 95% CI=0.270-0.790). Genotype GG and allele G in MMP7 rs11568818 polymorphism were associated with the risk of NSCLC development (P=0.024 and P=0.038). In addition, the interaction existed in gene polymorphsims and environment (P=0.011). Conclusion: AXIN2 rs2240307 and rs2240308 and MMP7 rs11568818 polymorphisms might be the independent risk factors for NSCLC and showed the interaction with environmental factor.
Xu T.,Second Peoples Hospital of Wuxi |
Fan B.,Second Peoples Hospital of Wuxi |
Lv C.,Second Peoples Hospital of Wuxi |
Xiao D.,Second Peoples Hospital of Wuxi
Oncology Reports | Year: 2015
Slug is involved in the radioresistance and chemoresistance of several types of cancers. In the present study, we first studied the effect of Slug on the radioresistance of nasopharyngeal carcinoma (NPC). We established radioresistant CNE-2 cells (CNE-2-RES) by exposing CNE-2 cells to gradually increasing doses of irradiation (IR). We used lentiviral infection technique to stably knock down Slug and then studied the effects in vitro and in vivo. Western blotting and RT-PCR were applied to detect the protein and mRNA expression in NPC cells or xenograft tumor tissues, respectively. Colony forming assay was applied to detect the cell survival after IR. As a result, CNE-2-RES cells were successfully established, CNE-2-RES cells showed relatively higher expression of Slug, higher expression of p53 and lower expression of PUMA. Following inhibition of Slug, the radiosensitivity of NPC was enhanced both in vitro and in vivo. Slug inversely regulated PUMA and p53 expression in both CNE-2 and CNE-2-RES cells. Animal experiments showed the same trend of protein expression as the in vitro results. In conclusion, our study demonstrated that Slug overexpression in CNE-2-RES cells may result in the radioresistance of cells. Slug mediates CNE-2 radioresistance via downregulation of PUMA in both a p53-dependent and p53-independent manner.
PubMed | Jiangsu University and Second Peoples Hospital of Wuxi
Type: | Journal: Cancer science | Year: 2016
Keratin8 (KRT8) is the major component of the intermediate filament cytoskeleton and predominantly expressed in simple epithelial tissues. Aberrant expression of KRT8 is associated with multiple tumor progression and metastasis. However, the role of KRT8 in Gastric Cancer (GC) remains unclear. In this study, KRT8 expression was investigated and it was found to be up-regulated along with human GC progression and metastasis at both mRNA and protein levels in human gastric cancer tissues. In addition, KRT8 over expression enhanced the proliferation and migration of human gastric cancer cells, whereas the knock-down of KRT8 by siRNA only inhibited migration of human gastric cancer cells. Integrin1-FAK-induced epithelial-mesenchymal-transition (EMT) only existed in the high KRT8 cells. Furthermore, KRT8 over expression led to increase in p-smad2/3 levels and TGF dependent signaling events. KRT8 expression in GC was related to tumor clinical stage and worse survival. Kaplan-Meier analysis proved that KRT8 was associated with overall survival of patients with GC that patients with high KRT8 expression tend to have unfavorable outcome. Moreover, coxs proportional hazards analysis showed that high KRT8 expression was a prognostic marker of poor outcome. These results provided that KRT8 expression may therefore be a biomarker or potential therapeutic target to identify patients with worse survival. This article is protected by copyright. All rights reserved.