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Zhang K.,Jiangsu Institute of Nuclear Medicine | Ren T.,Second Peoples Hospital of Wuxi | Wang K.,Jiangsu Institute of Nuclear Medicine | Zhu X.,Jiangsu Institute of Nuclear Medicine | And 2 more authors.
Chemical Communications | Year: 2014

A new Exo III assisted strand-cleavage cycle and ligand-responsive quadruplex formation strategy for amplified and label-free detection of IFN-γ was reported with a detection limit of 0.1 pM and a visual detection limit of 20 pM by the naked eye. © the Partner Organisations 2014.

Ji X.,Second Peoples Hospital of Wuxi | Li J.,Nanjing Medical University | Yang C.,Second Peoples Hospital of Wuxi
Journal of Immunoassay and Immunochemistry | Year: 2015

Herein, a simple and novel electrochemical method for the detection of potassium ions (K+) was developed. In the presence of potassium ions, the potassium ions aptamer will form a G-quadruplex complex. Thus, further addition of hemin in the presence of potassium ions will lead to the formation of a recombined G-quadruplex. Then the electroactive label, hemin, will give an electrochemical response. The linear range of the method covered a large variation of K+ concentration from 0.1 nM to 0.1 M and the detection limit of 0.1 nM was obtained. Moreover, this assay was able to detect K+ with high selectivity and had great potential applications. © 2015 Taylor & Francis Group, LLC.

Ren T.-L.,Second Peoples Hospital of Wuxi | Yang C.-J.,Second Peoples Hospital of Wuxi | Fang D.-Y.,Second Peoples Hospital of Wuxi | Shi G.-X.,Second Peoples Hospital of Wuxi | Zhu X.,Jiangsu Institute of Nuclear Medicine
Acta Crystallographica Section E: Structure Reports Online | Year: 2012

The asymmetric unit of the title ion-pair complex, (C9H 12NO2)2[Ni(C4N2S 2)2], contains two 1-(eth-oxy-carbonyl-meth-yl)pyridinium cations and one bis-(1,2-dicyano-ethene-1,2-dithiol-ato)nickelate(II) dianion, which exhibits a slightly distorted square-planar coordination geometry. In the crystal, the cations are linked by strong C - H⋯O hydrogen bonds into C(6) chains along [100]. The cations and anions are linked into a three-dimensional architecture by weak C - H⋯N and C - H⋯S interactions.

Xu T.,Second Peoples Hospital of Wuxi | Fan B.,Second Peoples Hospital of Wuxi | Lv C.,Second Peoples Hospital of Wuxi | Xiao D.,Second Peoples Hospital of Wuxi
Oncology Reports | Year: 2015

Slug is involved in the radioresistance and chemoresistance of several types of cancers. In the present study, we first studied the effect of Slug on the radioresistance of nasopharyngeal carcinoma (NPC). We established radioresistant CNE-2 cells (CNE-2-RES) by exposing CNE-2 cells to gradually increasing doses of irradiation (IR). We used lentiviral infection technique to stably knock down Slug and then studied the effects in vitro and in vivo. Western blotting and RT-PCR were applied to detect the protein and mRNA expression in NPC cells or xenograft tumor tissues, respectively. Colony forming assay was applied to detect the cell survival after IR. As a result, CNE-2-RES cells were successfully established, CNE-2-RES cells showed relatively higher expression of Slug, higher expression of p53 and lower expression of PUMA. Following inhibition of Slug, the radiosensitivity of NPC was enhanced both in vitro and in vivo. Slug inversely regulated PUMA and p53 expression in both CNE-2 and CNE-2-RES cells. Animal experiments showed the same trend of protein expression as the in vitro results. In conclusion, our study demonstrated that Slug overexpression in CNE-2-RES cells may result in the radioresistance of cells. Slug mediates CNE-2 radioresistance via downregulation of PUMA in both a p53-dependent and p53-independent manner.

Han S.,Second Peoples Hospital of Wuxi | Lv L.,Second Peoples Hospital of Wuxi | Wang X.,Second Peoples Hospital of Wuxi | Zhao H.,Second Peoples Hospital of Wuxi
International Journal of Clinical and Experimental Pathology | Year: 2016

Objectives: This study aimed to explore the effect of AXIN2 and MMP7 polymorphisms on non-small cell lung cancer (NSCLC) susceptibility; in addition, the interaction between gene polymorphisms and environment was also displayed. Methods: The genotyping was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 102 patients with NSCLC and 120 healthy controls. Odds ratio (OR) and 95% confidence interval (CI) were calculated to assess the relevance strength of AXIN2 and MMP7 polymorphisms with NSCLC. The χ2 test was used to compare to the frequencies difference of genotypes and alleles in cases and controls and Hardy-Weinberg equilibrium (HWE) test. The haplotype and interaction analyses were performed by haploview and MDR software, respectively. Results: The genotype frequencies of all polymorphisms in the control group conformed to HWE. GG genotype frequency of AXIN2 rs2240307 polymorphism was significantly higher in cases than controls (P=0.041). Similarly, rs2240308 in AXIN2 gene was also increased the susceptibility to NSCLC remarkably (OR=2.412, 95% CI=1.025-5.674). What's more, haplotype A-G-G in AXIN2 might play a protective role in NSCLC (OR=0.462, 95% CI=0.270-0.790). Genotype GG and allele G in MMP7 rs11568818 polymorphism were associated with the risk of NSCLC development (P=0.024 and P=0.038). In addition, the interaction existed in gene polymorphsims and environment (P=0.011). Conclusion: AXIN2 rs2240307 and rs2240308 and MMP7 rs11568818 polymorphisms might be the independent risk factors for NSCLC and showed the interaction with environmental factor.

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