Entity

Time filter

Source Type


Wang Z.,Peking University | Qi X.K.,Navy General Hospital | Yao S.,Navy General Hospital | Chen B.,Peking University | And 4 more authors.
Neuropathology | Year: 2010

The 13513G>A mutation in the ND5 gene of mitochondrial DNA (mtDNA) is usually associated with mitochondrial encephalomyopathy with lactate acidosis and stroke-like episodes (MELAS), or Leigh syndrome (LS). In this study, we describe three young Chinese patients with MELAS/LS overlap syndrome who carried the m.13513G>A mutation. Clinical and MRI features were characteristic of both MELAS and LS. Interestingly, the clinical presentation of this overlap syndrome could be variable depending on the degree of relative contribution of MELAS and LS, that is, MELAS as the initial presenting syndrome, LS as the predominant syndrome, or both MELAS and LS appearing at the same time. The final brain MRI showed findings characteristic of both MELAS and LS, with asymmetrical lesions in the cortex and subcortical white matter of the occipital, temporal, and frontal lobes (MELAS), and bilateral and symmetrical lesions in the basal ganglia and brainstem (LS). Brain autopsy in one case revealed infarct-like lesions in the cerebral cortex, basal ganglia and brainstem, providing further insight into the distribution of the pathological lesions in MELAS/LS overlap syndrome. This is the first report of the brain pathological changes in a patient with m.13513G>A mutation. The spatial distribution of infarct-like lesions in the brain could explain the symptoms in MELAS/LS overlap syndrome. © 2010 Japanese Society of Neuropathology. Source


Guo Y.-J.,Chinese Peoples Liberation Army | Liu J.-X.,Second Peoples Hospital of Shenzhen | Guan Y.-W.,Chinese Peoples Liberation Army
European Review for Medical and Pharmacological Sciences | Year: 2016

OBJECTIVE: Previous studies reported that NDRG2 might be a tumor suppressor of prostate cancer. In this study, we investigated the hypoxia-induced expression change of miR-301a/b in prostate cancer cells and explored its regulation on NDRG2 in autophagy and viability of prostate cancer cells. MATERIALS AND METHODS: MiR-301a/b expression in hypoxia and normoxia cultured prostate cancer cells was measured. Its regulation on autophagy was measured by quantifying expression change of LC3B and p62. The direct binding between miR-301a/b and 3'UTR of NDRG2 was verified using dual luciferase, qRT-PCR and Western blot assay. The influence of miR-301a/b-NDRG2 axis on autophagy, viability and apoptosis of prostate cancer cells was further investigated. RESULTS: Hypoxia induced a significant upregulation of miR-301a/b in prostate cancer cells. Enhanced miR-301a/b expression significantly weakened autophagy of prostate cancer cells. Both miR-301a and miR-301b could directly target 3'UTR of NDRG2 and decrease its expression. Decreased NDRG2 expression directly resulted in increased autophagy and cell viability and reduced cell apoptosis. CONCLUSIONS: Taken together, we demonstrated that miR-301a/b-NDRG2 might be an important axis modulating autophagy and viability of prostate cancer cells under hypoxia. Source


Liu L.-X.,Second Peoples Hospital of Shenzhen | Chen L.,Jiamusi Jinjue Institute for Dentistry
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Glass ionomer cements have been gradually employed in many aspects of dental clinical field. However, low mechanical strength and antibacterial effect restrict its further applications. OBJECTIVE: To proportionally explore the effects of titanium dioxide nanoparticles on the mechanical strength and antibacterial effect of traditional glass ionomer cements. METHODS: Base on different mass fractions of titanium dioxide nanoparticles in glass ionomer cements, all the glass ionomer cement samples were divided into control group (no titanium dioxide nanoparticles), low titanium dioxide group (containing 3% titanium dioxide nanoparticles), medium titanium dioxide group (containing 6% titanium dioxide nanoparticles), and high titanium dioxide group (containing 9% titanium dioxide nanoparticles). A universal material testing machine and a hardness tester were used to examine flexural strength, compressive strength, and surface hardness of glass ionomer cement samples, respectively. Glass ionomer cement samples were immerged into the artificial saliva, and fluoride release was measured by using a fluoride ion selective electrode. The direct contact test was used to investigate antibacterial effect of glass ionomer cement samples towards Streptococcus mutans. RESULTS AND CONCLUSION: Compared with the control group, few titanium dioxide nanoparticles (low and medium titanium dioxide group) could significantly improve flexural strength, compressive strength and surface hardness of glass ionomer cement samples (P < 0.05), and high titanium dioxide nanoparticles (high titanium dioxide group) significantly decreased flexural strength, compressive strength and surface hardness (P < 0.05). The introduction of titanium dioxide nanoparticles had little effect on fluorine release behavior of glass ionomer cement samples, and greatly improved antibacterial effect of glass ionomer cement samples towards Streptococcus mutans. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Liu L.-X.,Second Peoples Hospital of Shenzhen | Lin C.,Jinjue Dental Institute of Jiamusi
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Children teeth filled with glass ionomer cement are still susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes. OBJECTIVE: To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children. METHODS: Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filled index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescent in situ hybridization was used to investigate the numerical level of the caries-induced microbe Streptococcus spp. Quantitative PCR was carried out to analyze relative quantity of Streptococcus mutans in total bacteria. RESULTS AND CONCLUSION: Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries. Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios of Streptococcus spp. and Streptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can well reflect caries-related complex microbial community in dental plaque. Source


Zhang Y.,Sun Yat Sen University | Zhang Y.,Southern Medical University | Huang H.,Sun Yat Sen University | Zhou H.,Sun Yat Sen University | And 8 more authors.
Cancer | Year: 2014

BACKGROUND: Nuclear factor jB (NFκB) signaling is strongly associated with tumor progression, and studies have shown that SHANK-associated RH domain interacting protein (SHARPIN) is crucial for NFκB pathway activation. However, the expression and functions of SHARPIN in prostate cancer (PCa) have not yet been defined.METHODS: The expression of SHARPIN in PCa cell lines and tissues was evaluated with western blotting, quantitative real-time polymerase chain reaction, and immunohistochemistry. After SHARPIN was silenced in the PCa cell lines, western blots were used to confirm that SHARPIN physically associated with components of the NFκB pathway and the downstream targets (survivin and livin). The functions of SHARPIN in cell proliferation, migration, and invasion in vitro were measured with 5-(3-carboxymethoxyphenyl)-2-(4,5-dimenthylthiazoly)-3-(4-sulfophenyl)tetrazolium, inner salt (MTS), Transwell, and invasion assays, respectively. Flow cytometry was employed to evaluate cell apoptosis. Furthermore, tumorigenesis in vivo was examined with tumorigenicity assays.RESULTS: SHARPIN expression was upregulated in PCa cell lines and tissues. The knockdown of SHARPIN or incubation with Bay 11-7082 (an NFκB inhibitor) led to dramatically decreased levels of phosphorylated IjBa and phosphorylated p65 in comparison with the control group. Downregulation of survivin and livin due to SHARPIN inhibition was attributable to transcriptional repression (P<.05). Decreases in cell viability, migration, invasion, and survival with a higher sensitivity to docetaxel in vitro and with repressed tumorigenesis in vivo were observed upon SHARPIN silencing, and this was consistent with the results from inhibition of the NFκB pathway and its downstream targets.CONCLUSION: The current study demonstrates that overexpression of SHARPIN promotes activation of the NFκB pathway and downstream targets survivin and livin, which potentially contributes to PCa development. © 2014 American Cancer Society. Source

Discover hidden collaborations