Jiang O.,Second Peoples Hospital of Neijiang |
Yang K.,Second Peoples Hospital of Neijiang |
Cai C.-X.,Second Peoples Hospital of Neijiang |
Liu Y.,Second Peoples Hospital of Neijiang |
And 2 more authors.
World Journal of Gastroenterology | Year: 2016
AIM: To evaluate short-term outcomes following intraoperative biliary lavage for hepatolithiasis. METHODS: A total of 932 patients who were admitted to the West China Medical Center of Sichuan University between January 2010 and January 2014 and underwent bile duct exploration and lithotomy were retrospectively included in our study. The patients were divided into the lavage group and the control group. Related pre-, intra-, and postoperative factors were recorded, analyzed, and compared between the two groups in order to verify the effects of biliary lavage on the short-term outcome of patients with hepatolithiasis. RESULTS: Amongst the patients who were included, 678 patients with hepatolithiasis were included in the lavage group, and the other 254 patients were enrolled in the control group. Data analyses revealed that preoperative baseline and related intraoperative variables were not significantly different. However, patients who underwent intraoperative biliary lavage had prolonged postoperative hospital stays (6.67 d vs 7.82 d, P = 0.024), higher hospitalization fees (RMB 28437.1 vs RMB 32264.2, P = 0.043), higher positive rates of bacterial cultures from blood (13.3% vs 25.8%, P = 0.001) and bile (23.6% vs 40.7%, P = 0.001) samples, and increased usage of advanced antibiotics (26.3% vs 38.2%, P = 0.001). In addition, in the lavage group, more patients had fever (> 37.5 °C, 81.4% vs 91.1%, P = 0.001) and hyperthermia (> 38.5°C,39.7% vs 54.9%, P = 0.001), and higher white blood cell counts within 7 d after the operation compared to the control group. CONCLUSION: Intraoperative biliary lavage might increase the risk of postoperative infection, while not significantly increasing gallstone removal rate. ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Xie C.-M.,Southwest Jiaotong University |
Lu X.,Southwest Jiaotong University |
Wang K.-F.,University of Sichuan |
Meng F.-Z.,Second Peoples Hospital of Neijiang |
And 4 more authors.
ACS Applied Materials and Interfaces | Year: 2014
Research on incorporation of both growth factors and silver (Ag) into hydroxyapatite (HA) coatings on metallic implant surfaces for enhancing osteoinductivity and antibacterial properties is a challenging work. Generally, Ag nanoparticles are easy to agglomerate and lead to a large increase in local Ag concentration, which could potentially affect cell activity. On the other hand, growth factors immobilization requires mild processing conditions so as to maintain their activities. In this study, bone morphology protein-2 (BMP-2) and Ag nanoparticle contained HA coatings were prepared on Ti surfaces by combining electrochemical deposition (ED) of Ag and electrostatic immobilization of BMP-2. During the ED process, chitosan (CS) was selected as the stabilizing agent to chelate Ag ions and generate Ag nanoparticles that are uniformly distributed in the coatings. CS also reduces Ag toxicity while retaining its antibacterial activity. Afterwards, a BMP/heparin solution was absorbed on the CS/Ag/HA coatings. Consequently, BMP-2 was immobilized on the coatings by the electrostatic attraction between CS, heparin, and BMP-2. Sustained release of BMP-2 and Ag ions from HA coatings was successfully demonstrated for a long period. Results of antibacterial tests indicate that the CS/Ag/HA coatings have high antibacterial properties against both Staphylococcus epidermidis and Escherichia coli. Osteoblasts (OB) culture reveals that the CS/Ag/HA coatings exhibit good biocompatibility. Bone marrow stromal cells (BMSCs) culture indicates that the BMP/CS/Ag/HA coatings have good osteoinductivity and promote the differentiation of BMSCs. Ti bars with BMP/CS/Ag/HA coatings were implanted into the femur of rabbits to evaluate the osteoinductivity of the coatings. Results indicate that BMP/CS/Ag/HA coatings favor bone formation in vivo. In summary, this study presents a convenient and effective method for the incorporation of growth factors and antibacterial agents into HA coatings. This method can be utilized to modify a variety of metallic implant surfaces. © 2014 American Chemical Society.
Dang C.,Guangxi Medical University |
Dang C.,Second Peoples Hospital of Neijiang |
Yang Z.,Guangxi Medical University |
Li L.,Guangxi Medical University
Chinese Journal of Clinical Oncology | Year: 2012
Objective: This study analyzed the effects of clinical and pathological factors on surgery for recurrent epithelial ovarian cancer (EOC). Methods: Univariate and multivariate step-wise logistic regression analyses were used to compare clinical and pathological factors that influenced the effects of cytoreductive surgery on 60 cases with recurrent EOC. Results: The overall median survival time of the 60 patients with recurrent EOC was 26 months (95% CI: 18.302 to 33.698 months). The median survival time in the patients treated by optimal cytoreductive surgery was 28 months (95% CI: 25.043 to 30.957months). By contrast, the median survival time in the patients treated by suboptimal cytoreductive surgery was 16 months (95% CI: 13.184 to 18.816 months) (P=0.002). Univariate logistic regression analysis showed that the recurrence number, relapse with ascites, and the recurrence site are factors of the surgical effects for recurrent EOC (P<0.05). However, no relationships were observed among the following factors: age, initial surgery, pathological type, grade of tumor cells, FIGO (International Federation of Obstetrics and Gynecology) stage, initial chemotherapeutic program, recurrence time since the last chemotherapy, recurrent CA125 level, largest recurrence lesion, and prior chemotherapy (P>0.053). The logistic regression revealed that the recurrence number, recurrence site, relapse with ascites, and age are significant factors that gradually influence the effects of surgery for recurrent EOC. Conclusion: Multiple clinical and pathological factors will affect the results of surgery for recurrent EOC, The number and site of recurrence, as well as the age and relapse with ascites are independent risk factors.
Wang Z.,Key Laboratory of Advanced Technologies of Materials |
Wang K.,University of Sichuan |
Lu X.,Key Laboratory of Advanced Technologies of Materials |
Li M.,Shandong University |
And 6 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015
Bone morphology protein-2 (BMP-2) encapsulated chitosan/chondrotin sulfate nanoparticles (CHI/CS NPs) are developed to enhance ectopic bone formation on biphasic calcium phosphate (BCP) scaffolds. BMP-2 contained CHI/CS NPs were prepared by a simple and mild polyelectrolyte complexation process. It does not involve harsh organic solvents and high temperature, and therefore retain growth factors activity. These NPs were immobilized on BCP scaffolds, and realize the sustained release of growth factors from the scaffolds. The bare BCP scaffolds, NP loaded scaffolds (BCPNP), and NP loaded and polydopamine coated scaffolds (BCP-Dop-NP) were seeded with bone marrow stroma cells (BMSC) to evaluate the osteoinductivity of the scaffolds. BMSC culture results indicate that all scaffolds favor cell adhesion, proliferation, differentiation. Afterwards, the bare BCP, BCP-NP, and BCP-Dop-NP scaffolds were implanted into rabbits intramuscularly to evaluate the ectopic bone formation of scaffolds. In vivo results indicate that the BCP-NP and BCP-Dop-NP scaffolds enhance more ectopic bone formation than the bare BCP scaffolds. Both the in vitro and in vivo results demonstrate that BMP-2 encapsulated polysaccharide NPs are effective to improve the osteoinductivity of the scaffolds. In addition, BCP-NP scaffolds induce more bone formation than BCP-Dop-NP scaffolds. This is because BCP-NP scaffolds harness the intrinsic osteoinductivity BCP and BMP-2, whereas BCP-Dop-NP scaffolds have polydopamine coatings that inhibit the surfaces biological features of BCP scaffolds, and therefore weaken the bone formation ability of scaffolds. © 2014 Wiley Periodicals, Inc.
Su P.,Second Peoples Hospital Of Neijiang |
Zhang W.-W.,Second Peoples Hospital Of Neijiang |
Yu H.-W.,Second Peoples Hospital Of Neijiang |
Yang H.,University of Sichuan
Chinese Journal of Tissue Engineering Research | Year: 2015
Background: Insufficient source of seed cells is the important factor to restrict the tissue reconstruction and the development of regenerative medicine. objective: To explore the osteogenesis of adipose stem cells cultured with different kinds of artificial bones. Methods: Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cells. Passage 4 adipose-derived mesenchymal stem cells were selected and divided into osteogenic induction group, osteogenic induction+hydroxyapatite group, osteogenic induction+absorbable bone group and osteogenic induction+recombinant bone xenograft group. The latter three groups were subdivided int 3,10, 20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, totally for 12 days. Results And Conclusion: Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction+hydroxyapatite group and low-concentration osteogenic induction+absorbable bone group (both P < 0.05), but no difference was found between the osteogenic induction group and high-concentration osteogenic induction+absorbable bone group (P< 0.05). In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction+ recombinant bone xenograft group than the osteogenic induction (P < 0.05). Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cells, and among them, hydroxyapatite has a better effect an the osteogenic induction af adipose stem cells. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved.
Zeng R.,University of Sichuan |
Xu C.-H.,Second Peoples Hospital of Neijiang |
Xu Y.-N.,University of Sichuan |
Wang Y.-L.,University of Sichuan |
Wang M.,University of Sichuan
Public Health Nutrition | Year: 2015
Objective Folate and vitamin B12 are two vital regulators in the metabolic process of homocysteine, which is a risk factor of atherothrombotic events. Low folate intake or low plasma folate concentration is associated with increased stroke risk. Previous randomized controlled trials presented discordant findings in the effect of folic acid supplementation-based homocysteine lowering on stroke risk. The aim of the present review was to perform a meta-analysis of relevant randomized controlled trials to check the how different folate fortification status might affect the effects of folic acid supplementation in lowering homocysteine and reducing stroke risk. Design Relevant randomized controlled trials were identified through formal literature search. Homocysteine reduction was compared in subgroups stratified by folate fortification status. Relative risks with 95 % confidence intervals were used as a measure to assess the association between folic acid supplementation and stroke risk. Setting The meta-analysis included fourteen randomized controlled trials, Subjects A total of 39 420 patients. Results Homocysteine reductions were 26·99 (sd 1·91) %, 18·38 (sd 3·82) % and 21·30 (sd 1·98) %, respectively, in the subgroups without folate fortification, with folate fortification and with partial folate fortification. Significant difference was observed between the subgroups with folate fortification and without folate fortification (P=0·05). The relative risk of stroke was 0·88 (95 % CI 0·77, 1·00, P=0·05) in the subgroup without folate fortification, 0·94 (95 % CI 0·58, 1·54, P=0·82) in the subgroup with folate fortification and 0·91 (95 % CI 0·82, 1·01, P=0·09) in the subgroup with partial folate fortification. Conclusions Folic acid supplementation might have a modest benefit on stroke prevention in regions without folate fortification. Copyright © The Authors 2014.
Han L.,Southwest Jiaotong University |
Lin H.,Southwest Jiaotong University |
Lu X.,Southwest Jiaotong University |
Zhi W.,Southwest Jiaotong University |
And 3 more authors.
Materials Science and Engineering C | Year: 2014
Bone morphogenic protein-2 (BMP2)-encapsulated chitosan (CS) coatings were prepared to immobilize BMP2 on titanium (Ti) surfaces. The Ti substrates were functionalized through a three-step process: alkali treatment, silanization with 3-aminopropyltriethoxysilane and aldehydation with glutaraldehyde (GA). BMP2-encapsulated CS coatings (BMP2-CS) were bonded to Ti surfaces through reactions between the aldehyde groups of GA and the amine groups of CS. Direct BMP2 immobilization on aldehyde-treated Ti (BMP2-Ti) and pure CS coatings (CS-Ti) were used as controls. The release rate of BMP2-CS-Ti was half of that of BMP2-Ti at initial stage, which indicates that the CS coatings are suitable carriers for sustained BMP2 release. The osteoinductivities of BMP2-CS-Ti, BMP2-Ti, CS-Ti and pristine Ti were examined by both in vitro cell tests and in vivo experiments. Bone marrow stem cell (BMSC) culture indicated that BMP2-CS-Ti is more potent in stimulating the differentiation of the adhering BMSC than the three other groups. Rabbit femur implantation revealed the excellent osteoinductivity of BMP2-CS-coated Ti implants. These results demonstrate that the BMP2-encapsulated CS coatings are stable osteoinductive coatings that realize the sustained release of BMP2 and maintain the activity of the protein. © 2014 Elsevier B.V.