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Gu J.,Nanjing Medical University | Shi W.,Second Peoples Hospital of Nantong | Lu Y.,Nanjing Medical University | Zhu Q.,Nanjing Medical University | And 5 more authors.
International Immunopharmacology | Year: 2015

Graft-versus-host disease (GVHD) is an intractable complication in transplant patients. Regulatory T cells (Tregs) have the ability to prevent GVHD and consist of two subsets: natural Tregs (nTregs) and induced Tregs (iTregs). In comparison to nTregs, iTregs originate in the periphery under certain conditions and show improved proliferative and suppressive abilities in an inflammatory milieu. All-trans retinoic acid (atRA) favors Treg expansion and FoxP3 expression in human Tregs. However, whether atRA can affect the function of iTregs from transplant patients remains inconclusive. Therefore, we sorted naïve T cells from liver transplant patients and cultured them in vitro. Further analyses were performed to assess the suppressive function of iTregs in vitro and in vivo. atRA favored expansion and forkhead box P3 expression in iTregs from transplant patients. In comparison to iTregs from healthy donors, iTregs from transplant patients showed decent suppressive abilities in vitro and in vivo. Our findings suggest that atRA can potentially improve the development and function of iTregs from transplant patients. Furthermore, our results provide novel insights into Treg therapy in GVHD clinical trials. © 2015 Elsevier B.V. Source


Wu F.,Soochow University of China | Wu F.,Nantong University | Wang Z.,Soochow University of China | Gu J.-H.,Soochow University of China | And 4 more authors.
Neurochemistry International | Year: 2013

Rotenone is an environmental neurotoxin that induces degeneration of dopaminergic (DA) neurons in substantia nigra pars compacta (SNpc), which ultimately results in parkinsonism, but the molecular mechanisms of selective degeneration of nigral DA neurons are not fully understood. In the present study, we investigated the induction of p38MAPK/p53 and Bax in SNpc of Lewis rats after chronic treatment with rotenone and the contribution of Bax to rotenone-induced apoptotic commitment of differentiated PC12 cells. Lewis rats were subcutaneously treated with rotenone (1.5 mg/kg) twice a day for 50 days and the loss of tyrosine hydroxylase (THase), motor function impairment, and expression of p38MAPK, P-p38MAPK, p53, and Bax were assessed. After differentiated PC cells were treated with rotenone (500 nM) for 6-36 h, protein levels of p38MAPK and P-p38MAPK, p53 nuclear translocation, Bax induction and cell death were measured. The results showed that rotenone administration significantly reduced motor activity and caused a loss of THase immunoreactivity in SNpc of Lewis rats. The degeneration of nigral DA neurons was accompanied by the increases in p38MAPK, P-p38MAPK, p53, and Bax protein levels. In cultured PC12 cells, rotenone also induced an upregulation of p38MAPK, P-p38 MAPK, p53 and Bax. Pharmacological inhibition of p38MAPK with SB203580 (25 μM) blunted rotenone-induced cell apoptosis. Treatment with SB203580 prevented the p53 nuclear translocation and upregulation of Bax. Inhibition of p53 with pifthrin-alpha or Bax with siRNAs significantly reduced rotenone-induced Bax induction and apoptotic cell death. These results suggest that the p38MAPK/p53-dependent induction of Bax contributes to rotenone's neurotoxicity in PD models. © 2013 Elsevier Ltd. All rights reserved. Source


Ge J.-F.,Second Peoples Hospital of Nantong | Cao S.-F.,Second Peoples Hospital of Nantong
Journal of Clinical Dermatology | Year: 2014

Objective: To summarize the experience of diagnosis and treatment of scrub typhus, and to reduce the rate of misdiagnosis and missed diagnosis. Methods: The medical records of 15 inpatients from November 2010 to December 2011 were analyzed. Results; All patients had different degrees of fever, some patients had liver, lung or kidney damages; 14 cases had typical eschar or specific ulcer. Treatment with doxycycline obtained good results. Conclusion; Scrub typhus is of various clinical manifestations, detailed medical history investigation and physical examination are very important in reducing misdiagnosis and missed diagnosis. Doxycycline and azithromycin are effective drugs for treating scrub typhus. Source


Sun X.,Second Peoples Hospital of Nantong | Huang X.,Second Peoples Hospital of Nantong | Zhao R.,Second Peoples Hospital of Nantong | Chen B.,Second Peoples Hospital of Nantong | Xie Q.,Second Peoples Hospital of Nantong
Pancreatology | Year: 2015

Background and aim Questions remain unclear about the association of smoking status and the development of acute pancreatitis (AP). We performed a meta-analysis of observational studies explore this association. Methods A computerized literature search was performed in MEDLINE and EMBASE through November 30, 2014. We also searched the reference lists of pertinent articles. We used a random-effects model to calculate the summary relative risks (SRRs) and their corresponding 95% confidence intervals (CIs). Results A total of 3690 incident cases of AP included 12 observational studies (6 case-control and 6 prospective cohort/nested case-control studies) were identified. Compared with never smokers, the summary RR estimates were 1.54 (95% CI, 1.31-1.80) for ever smokers, 1.71 (95% CI, 1.37-2.14) for current smokers, and 1.21 (95% CI, 1.02-1.43) for former smokers. Smoking is found to be a potential risk factor for alcohol use, idiopathic factors and drugs related AP, but not for gallstone related AP, in the ever and current smokers. A dose-response effect of tobacco use on the risk was ascertained: current smokers had a 40% (95% CI, 30%-51%) increased risk of AP for every additional 10 cigarettes per day. Conclusion The present analysis suggests that smokers have an elevated risk of AP development. Further studies, however, are warranted before definitive conclusions can be drawn. © 2015 IAP and EPC. Source

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