Zhu P.,Fujian Medical University |
Lin H.,Fujian Medical University |
Lin H.,Xiamen Medical College |
Sun C.,Fujian Medical University |
And 6 more authors.
Molecular Medicine Reports | Year: 2012
The aim of this study was to investigate the protective effects of telmisartan and/or pyridoxamine on spontaneously hypertensive rats (SHRs). Rats were treated with telmisartan (T group) or pyridoxamine (P group), or telmisartan and pyridoxamine (TP group). The serum levels of advanced glycation end products (AGEs), superoxide dismutase (SOD), malonaldehyde and the level of 24-h urinary albumin were measured. Morphological changes in renal tissues were observed under light (H&E or Masson's trichrome) and transmission electron microscopy. Expression of NF-κBp65 and p-ERK1/2 in renal tissue was detected by immunohistochemistry. Expression of receptors for advanced glycation end products (RAGE) and TGF-β in the renal cortex was investigated by western blotting. We found that early renal structural and functional damage was alleviated in the three intervention groups. SOD activity was significantly elevated in the P and TP groups (P<0.05) compared to that in the T group. Of note, both the positive expression of NF-κBp65 (P<0.01 vs. the T and P groups) and p-ERK1/2 (P<0.05 vs. the P group) was lowest in the TP group. Our results suggest that the combined use of telmisartan and pyridoxamine is superior to the single use of either drug on renoprotection, which may result from the alleviation of oxidative stress and the reduction of NF-κBp65 and p-ERK1/2 activation.
Zhu H.-C.,Nanjing Medical University |
Yang X.,Nanjing Medical University |
Xu L.-P.,Nanjing Medical University |
Zhao L.-J.,Nanjing Medical University |
And 9 more authors.
Digestive Diseases and Sciences | Year: 2014
Background We conducted a systematic review and meta-analysis of meat intake and esophageal cancer risk, with subgroup analyses based on meat type and histological type of cancer. Aims The purpose of this study was to investigate the association between meat intake and risk of esophageal cancer. Methods We searched MEDLINE, EMBASE and Cochrane Library (April 2013) for cohort and case-control studies that assessed meat intake and esophageal cancer risk. Random-effect or fixed-effect models were used to pool relative risks (RRs) from individual studies with heterogeneity and publication bias analyses carried out. Seven cohort and 28 case-control studies were included. Results The summary RRs for esophageal cancer for the highest versus lowest consumption categories were 1.19 (95 % confidence interval [CI] 0.98-1.46) for total meat, 1.55 (95 % CI 1.22-1.96) for red meat, 1.33 (95 % CI 1.04-1.69) for processed meat, 0.72 (95 % CI 0.60-0.86) for white meat, 0.83 (95 % CI 0.72-0.96) for poultry, and 0.95 (95 % CI 0.76-1.19) for fish. When striated by histological subtype, positive associations were seen among esophageal squamous cell carcinoma and red meat, white meat and poultry, and esophageal adenocarcinoma with total meat and processed meat. Conclusions Meat consumption is associated with esophageal cancer risk, which depends on meat type and histological type of esophageal cancer. High intake of red meat and low intake of poultry are associated with an increased risk of esophageal squamous cell carcinoma. High meat intake, especially processed meat, is likely to increase esophageal adenocarcinoma risk. And fish consumption may not be associated with incidence of esophageal cancer. © Springer Science+Business Media New York 2013.
Zhang S.,Nanjing Medical University |
Zhang S.,Second Peoples Hospital of Lianyungang |
Xu K.,Nanjing Medical University |
Yang N.,Xuzhou Medical College |
Li C.,Nanjing Medical University
Nigerian Journal of Clinical Practice | Year: 2016
A number of treatment strategies for complex coronary lesions have been utilized in varying clinical settings over the last decade. However, cardiologists still encounter some difficult scenarios such as variant coronary artery origins, severely calcified and highly tortuous lesions. We report four cases in which the stents failed to cross lesions using the conventional percutaneous coronary intervention techniques, but all the target lesions were successfully stented finally using a new combined technique of anchor balloon assisted deep intubation of 5F 'child-in-mother' catheter. © 2016 Nigerian Journal of Clinical Practice | Published by Wolters Kluwer - Medknow.
Jiang J.-D.,Second Peoples Hospital of Lianyungang |
Zhou Y.,Second Peoples Hospital of Lianyungang |
Qian L.,Second Peoples Hospital of Lianyungang |
Bian G.-R.,Second Peoples Hospital of Lianyungang |
And 3 more authors.
Agro Food Industry Hi-Tech | Year: 2015
Hyperlipidaemia is one of major risk factors for the development of coronary artery diseases and atherosclerosis and therapeutic drugs must be developed for it. Lycium barbarum fruit is used in traditional Chinese medicine. In this study, L. barbarum oligosaccharides (LBOs) were prepared through hydrolysis with hydrogen peroxide (H2O2) and their hypolipidaemic activities were evaluated. A total of 24 male Sprague-Dawley rats were categorised into three treatment groups fed with high fat, LBOs and normal-fat diets, respectively. Oral administration of LBOs decreased body weight gain, total cholesterol level, low-density lipoprotein cholesterol level and liver weight. LBOs diet also increased the levels of faecal fat, cholesterol and plasma high-density lipoprotein cholesterol, as well as the activity of liver and serum lipoprotein lipases. Results suggested that LBOs exhibited high hypolipidaemic activity and could be explored as possible therapeutic agents for hyperlipidaemia.
Cui X.,Second Peoples Hospital of Lianyungang |
Meng Q.,Jiangsu University |
Chu Y.,Imperial College London |
Gu X.,Jiangsu University |
And 5 more authors.
RSC Advances | Year: 2016
Glucagon-like peptide-1 (GLP-1) and GLP-1 receptor agonists are actively pursued as therapeutic agents for type 2 diabetes mellitus (T2DM). However, the therapeutic utility of GLP-1 is limited due to its rapid inactivation by dipeptidyl peptidase IV, and many GLP-1 receptor agonists suffer from innegligible adverse effects. In the present study, in order to develop long-acting GLP-1 derivatives with improved hypoglycemic activity, native GLP-1 (7-36)-NH2 was loaded into sterically stabilized phospholipid micelles (SSM), affording GLP-1-SSM. In vitro stability test and in vivo pharmacokinetic study demonstrated that the association of GLP-1 with SSM led to enhanced stability and drug utilization without affecting its insulinotropic and glucose-lowering activities. Single and multiple glucose tolerance tests confirmed that GLP-1-SSM was a long-acting antidiabetic agent comparable or even better than exendin-4. More importantly, preclinical studies found out that a chronic twice daily treatment of GLP-1-SSM in type 2 diabetic db/db mice suppressed body weight gain and food uptake, decreased HbA1c value, and restored the glucose tolerance ability. Collectively, our results suggest that GLP-1-SSM is a promising therapy for the treatment of T2DM and deserves further investigation. © 2016 The Royal Society of Chemistry.
Qian L.,Second Peoples Hospital of Lianyungang |
Zhou Y.,Second Peoples Hospital of Lianyungang |
Ma J.-X.,Second Peoples Hospital of Lianyungang
International Journal of Biological Macromolecules | Year: 2014
This study was performed to investigate the hypolipidemic effect of the polysaccharides extracted from Porphyra yezoensis. Male Sprague-Dawley rats were divided into three groups and orally treated with diets containing either high fat, P. yezoensis polysaccharides (PPs), or normal fat. Treatment of male Sprague-Dawley rats with PPs led not only to significant decreases in plasma triacylglycerol, total cholesterol, and plasma low-density lipoprotein cholesterol and an increase in plasma high-density lipoprotein cholesterol, but also to significant decreases in liver weight, triacylglycerol and cholesterol. Therefore, the results suggest that PPs had a high hypolipidemic activity and could be used as a potential therapeutic agent for hyperlipidemia. © 2014 Elsevier B.V.
Chen J.-C.,Second Peoples Hospital of Lianyungang |
Sun D.-J.,Second Peoples Hospital of Lianyungang |
Ge Z.-L.,Second Peoples Hospital of Lianyungang
Journal of Clinical Neurology | Year: 2010
Objective: To investigate the effects of intensively antihypertensive therapy by Urapidil on hematoma enlargement in patients with hypertensive intracerebral hemorrhage(HICH). Methods: Hundred and four-six HICH patients were randomly divided into intensively antihypertensive group (intensive group, 75 cases, Urapidil was intravenous injected by micro-pump, the target value of mean arterial pressure was 110 mmHg) and the conventional antihypertensive group (control group, 71 cases, Captopril was taken, the target value of mean arterial pressure was 130 mmHg). The changes of hematoma volume and score of NIHSS were observed after treatment, as well as mortality and adverse drug reaction. Results: Twenty-four h after treatment in intensive group, the average volume of hematoma [(11.9 ± 9.5) ml], the rate of hematoma enlargement (14.7%, 11 cases), and the score of NIHSS (7.2 ± 5.0) were significantly differences compared with the control group [(13.2 ± 9.9) ml, (31.0%, 22 cases), (8.9 ± 6.8) scores] (all P < 0.05). Thirty d after onset, the score of NIHSS (4.6 ± 3.4) in intensive group was significant lower than control group (5.7 ± 4.1) (P < 0.01); the mortality rate (5.3%) was lower than control group (9.9%), but the difference was no statistically significant. The adverse reactions between the two groups was no significant different. Conclusion: Therapy of Urapidil intensive antihypertension can prevent hematoma enlargement of HICH patients, and the method is safe and reliable.
Liu Y.,Second Peoples Hospital of Lianyungang |
Yu J.,Second Peoples Hospital of Lianyungang |
Bai J.,Second Peoples Hospital of Lianyungang |
Gu J.-S.,Second Peoples Hospital of Lianyungang |
And 2 more authors.
Chinese Journal of Burns | Year: 2013
Objective: To study the effects of cuttlefish bone-bone morphogenetic protein (BMP) composite material on osteogenesis and revascularization of bone defect in rats. Methods: The cuttlefish bone was formed into cylinder with the diameter of about 5 mm and height of about 2 mm after the shell was removed, and then it was soaked in the recombinant human BMP 2 to make a cuttlefish bone-BMP (CBB) composite material. Thirty SD rats, with a defect of skull in every rat, were divided into the CBB and pure cuttlefish bone (PCB) groups according to the random number table, with 15 rats in each group. The rats in the group CBB and group PCB were transplanted with the corresponding material to repair the skull defect. At post transplantation week (PTW) 4, 6, and 8, 5 rats from every group were sacrificed by exsanguination, and ink perfusion was performed. One day later, all the transplants and part of the skull surrounding the defect were harvested, and general observation was conducted at the same time. The specimens were paraffin sectioned for HE staining and Masson staining. The area of microvessel and the area of newborn bone were observed and analyzed through histopathological techniques and image collection system. Data were processed with the analysis of variance of factorial design and LSD test. The correlation between the area of microvessel and the area of newborn bone of the group CBB was analyzed with Pearson correlation analysis. Results: (1) The general observation of the transplant region showed that the transplants were encapsulated by a capsule of fibrous connective tissue. The texture of capsule was soft and relatively thick at PTW 4. The texture was tenacious and thin, but rather compact at PTW 6 and 8. The transplants became gelatinous at PTW 4, and similar to the cartilage tissue at PTW 6 and 8. (2) Histological observation showed that the structure of the transplants in two groups was damaged at PTW 4. A moderate quantity of inflammatory cell infiltration could be observed. The amounts of the primary bone trabeculae and microvessels in group CBB were more abundant than those of group PCB, while the number of osteoclasts was less than those of group PCB. At PTW 6, the inflammatory cell infiltration in the transplants in both groups decreased obviously, the cuttlefish bone was found to be further degraded, and the number of newborn microvessels was increased. There were mature bone trabeculae around the transplants in both groups. And there were also mature bone trabeculae in the degraded CBB in group CBB. At PTW 8, the inflammatory reaction in the transplants in both groups disappeared; there were more mature bone trabeculae; the structure of the cuttlefish bone was found to be damaged basically. Bone trabeculae in group PCB were found around the transplant, while the bone trabeculae could be observed not only around the transplant but also in the degraded CBB in group CBB. The amount of the microvessels in group CBB was still larger than that of group PCB. (3) From PTW 4 to 8, the area of microvessel in group CBB [(63 ± 4), (136 ± 36), (347 ± 31) μm2] was larger than that in group PCB [(44 ± 7), (73 ± 4), (268 ± 42) μm2, P < 0.05 or P < 0.01]. From PTW 4 to 8, the area of newborn bone in group CBB [(236 ± 26), (339 ± 42), (553 ± 40) μm2] was larger than that in group PCB [(137 ± 15), (243 ± 21), (445 ± 29) μm2, with P values all below 0.01]. (4) The relation between the area of microvessel and the area of newborn bone was significantly positive (r = 0.948, P = 0.001). Conclusions: The CBB may exert good effect on osteogenesis and vascularization of rats with bone defect. It is a good three dimensional scaffold in bone tissue engineering.
Tian C.-R.,Second Peoples Hospital of Lianyungang |
Qian L.,Second Peoples Hospital of Lianyungang |
Shen X.-Z.,Second Peoples Hospital of Lianyungang |
Li J.-J.,Second Peoples Hospital of Lianyungang |
Wen J.-T.,Second Peoples Hospital of Lianyungang
PLoS ONE | Year: 2014
The serum total protein levels of the elderly possibly decrease gradually with aging. However, serum total protein levels are not suitable as a uniform reference standard for the elderly at different ages and genders. Thus, we investigated the total serum protein distribution in different gender and age groups of 11,453 elderly individuals aged ≥60 years and without liver or renal disease from Lianyungang, Jiangsu, China. The total protein levels (TPL) of these individuals exhibited normal distribution (Z = 1.206, P = 0.109), whereas the reference range (95% CI) was 54.1 g/L to 82.3 g/L. TPL was higher in females than in males for those aged between 60 and 75 years, whereas no significant difference was observed for those aged between 80 and 95 years. TPL was negatively correlated with age in males (r = -0.1342, P<0.05), females (r = -0.304, P<0.05), and the total group (r = -0.2136, P<0.05). TPL also decreased with aging and showed a faster rate in women than in men. These results indicated that an appropriate range of serum total protein based on age and gender differences should be used for clinical applications. © 2014 Tian et al.
Xu J.,Xuzhou Medical College |
Zhang Y.,Xuzhou Medical College |
Wei H.,Second Peoples Hospital Of Lianyungang |
Xu Y.,Xuzhou Medical College |
And 3 more authors.
Neurological Research | Year: 2015
Background and purpose: Thrombolytic treatment criteria vary significantly between China and the USA. We reviewed current intravenous (IV) thrombolytic therapy practices in China and the USA to determine the most appropriate.Methods: We conducted a systematic review of studies that used IV recombinant tissue plasminogen activator (rt-PA) therapy in China and the USA published between January 1950 and April 2012.Results: Literature search identified 17 American and 9 Chinese studies with a total of 2545 subjects. We found a significantly lower mortality rate in the US data compared with China (8% versus 13%; Chi-square = 24.412, P < 0.001). Our meta-regression analysis uncovered significant factors influencing mortality including male sex, hypertension, high cholesterol, smoking, and onset to treatment time (all P < 0.05). There were significantly more favorable outcomes in China than in the USA (61% versus 49%, Chi-square = 19.159, P < 0.001). No prior history of stroke and shorter onset to IV time were also significantly associated with a favorable outcome (P < 0.05).Conclusions: Onset to IV time is critical for reducing mortality and improving favorable outcomes. We suggest Chinese acute ischemic stroke treatment guidelines be revised to include an increase in the age limit of 80 years, removing contraindications such as a history of previous sever heart, liver, and kidney dysfunction, and placing more emphasis on physician expertise. © W. S. Maney & Son Ltd 2015.