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Li W.,Shandong University | Song A.-P.,Second Peoples Hospital of Jinan | Zhao F.,Cornell University | Hu Y.-M.,Shandong University | Hua M.,Chinese Institute of Scientific and Technical Information
Oncology Reports | Year: 2013

Transforming growth factor (TGF)-β-inducible nuclear protein 1 (TINP1) is a novel gene, which is localized at chromosome 5q13 where frequent abnormalities in hairy cell leukemia (HCL) occur. The present study investigated the effects of TINP1 knockdown or overexpression on the viability and gene expression of various tumor cell lines. siTINP1 was designed to knock down TINP1 expression. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to assess gene expression; the cell counting kit-8 (CCK-8) assay was used to detect cell viability, and luciferase and flow cytometry assays were used to determine gene activity. TINP1 was widely expressed in various cell lines. In addition, TINP1 siRNA was able to knock down TINP1 expression in HeLa cells. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

Sun L.,Harbin Medical University | Wang Q.,Second Peoples Hospital of Jinan | Gao X.,Harbin Medical University | Shi D.,Harbin Medical University | And 2 more authors.
FEBS Letters | Year: 2015

MicroRNAs (miRNAs) regulate gene expression by targeted repression of transcription and translation, and are involved in carcinogenesis. In this study, we demonstrated that the expression of miR-454 was up-regulated in uveal melanoma tissues compared to normal tissues. Ectopic expression of miR-454 resulted in significant promotion of cell proliferation, colony formation, invasion and induction of cell cycle in uveal melanoma cells. Furthermore, we identified PTEN as a direct target of miR-454. Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells. These findings support an oncogene role of miR-454 in development of uveal melanoma. © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Zhu C.-B.,Shandong University | Chen L.-L.,Second Peoples Hospital of Jinan | Tian J.-J.,Shandong University | Su L.,Shandong University | And 3 more authors.
Annals of Surgical Oncology | Year: 2012

Background. YKL-40 is a member of the mammalian chitinase-like proteins. Elevated serum YKL-40 levels in patients with gastrointestinal cancer at time of diagnosis are associated with poor prognosis. The aim of this study is to evaluate the prognostic value of serum YKL-40 before surgery and during follow-up in hepatocellular carcinoma (HCC) patients receiving curative resection. Methods. Serum YKL-40 levels were determined by enzyme-linked immunosorbent assay. Overall and recurrence-free survival (RFS) curves were constructed using the Kaplan-Meier method and compared by the log-rank test. A Cox proportional-hazards regression model was performed to identify independent prognostic factors. Median follow-up time was 35 months. Results. Baseline serum YKL-40 was elevated in 56% of patients with HCC receiving curative resection. Patients with elevated serum YKL-40 had significantly shorter overall and RFS than patients with normal serum YKL-40 (P = 0.003 and P = 0.001, respectively). Multivariate Cox regression analyses indicated that baseline serum YKL-40 was an independent prognostic variable for overall and RFS [hazard ratio (HR) = 1.968, 95% confidence interval (CI): 1.093-3.543, P = 0.024; HR = 1.891, 95% CI: 1.106-3.232, P = 0.020; respectively]. After curative resection, high serum YKL-40 (log-transformed continuous variable) within 6 months predicted significantly poorer overall survival (HR = 3.003, 95% CI: 1.323-6.817, P = 0.009). Conclusions. This study indicated that serum YKL-40 was an independent prognostic factor for overall and RFS in HCC patients receiving curative resection. Serial monitoring of serum YKL-40 after curative resection may provide prognostic information. © Society of Surgical Oncology 2011.

Sheng Y.-J.,Second Peoples Hospital of Jinan | Wang Y.,Second Peoples Hospital of Jinan
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2013

Diabetic macular edema (DME) is a leading cause of vision loss in diabetic patients. It is very important to correctly select a treating approach for DME. At present, the treating methods of DME include retinal laser photocoagulation, application of the glucocorticoid, intravitreous injection of anti-vascular endothelial growth factor (VEGF) drugs, administration of inhibitor of protein kinase C, vitrectomy and combined treatment etc. However, each method has its advantage and disadvantage. Retinal photocoagulation, vitrectomy, intravitreous injection and drug delivery system implantation are invasive treatment methods, and they can not rescue damaged retinal photoreceptors. Therefore, it is recommended that DME should be early diagnosed and effective treatment. The research status at home and abroad and future development trends of DME treatment were summarized. Copyright © 2013 by the Chinese Medical Association.

Zhang Y.,Second Peoples Hospital of Jinan | Li Z.,Shandong University | Liu L.,Second Peoples Hospital of Jinan | Han X.,Second Peoples Hospital of Jinan | And 2 more authors.
BioMed Research International | Year: 2014

Purpose. To compare the biomechanical properties of porcine, rabbit, and human sclera before and after riboflavin/ultraviolet-A (UVA) collagen cross-linking (CXL). Methods. Eight rabbits, 8 porcine eyeballs, and 8 human eyeballs were included. One rabbit eye and half of each bisected human and porcine eyeball were treated with riboflavin/UVA CXL. Untreated fellow rabbit eyes and eyeball halves served as controls. A 10 mm × 20 mm scleral band was harvested from each specimen. From this band, two 3.5 mm × 15.0 mm strips were prepared for biomechanical testing. The biomechanical parameters were ultimate stress, stress and Young's modulus. Results. Values of stress, and Young's modulus showed that human sclera was 4 times stiffer than porcine sclera and 3 times stiffer than rabbit sclera. In rabbit sclera, both the stress and Young's modulus were significantly increased by CXL (P < 0.05). In porcine sclera, only the ultimate stress was significantly increased by CXL (P < 0.05). The biomechanical properties of human sclera were not statistically affected by CXL (P > 0.05). Conclusions. Human sclera has higher biomechanical stiffness than porcine and rabbit sclera. With the same irradiation dose, riboflavin/UVA CXL increases the biomechanical stiffness of rabbit sclera but not porcine or human sclera. © 2014 Yali Zhang et al.

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