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Liu J.,Second Peoples Hospital of Jinan | Zhang M.,Second Peoples Hospital of Jinan | Li B.,Second Peoples Hospital of Jinan | Wang J.,Second Peoples Hospital of Jinan
Journal of Ophthalmology | Year: 2017

Purpose. To evaluate primary trabeculectomy with adjunctive mitomycin-C (MMC) in diabetic patients without retinopathy with primary angle-closure glaucoma (PACG). Design. This is a retrospective case series comparison. Participants. This retrospective trial compared outcomes of 88 eyes that underwent trabeculectomy in patients with diabetes mellitus (DM) without retinopathy and in 97 patients without DM. Methods. In this study, the intraocular pressure (IOP), visual acuity, visual field, and postoperative complications were compared between the two groups. Qualified surgical success is defined as an IOP between 6 and 18 mmHg with or without topical antiglaucoma medication. Results. After a follow-up of 5 years, the IOP decreased from a mean basal IOP of 27.8±7.3 mmHg to 15.0±5.6 mmHg in the DM group and from 27.3±6.0 mmHg to 12.4±5.3 mmHg in the control group. The mean number of antiglaucoma medications was 3.4±1.3 and 3.3±1.2 preoperatively (P=0.587) whereas it was 1.7±1.5 and 1.1±1.4 at the 5-year follow-up (P=0.049). The 5-year qualified surgical success rates were 42.9% and 65.4% for both groups (P=0.046; log-rank test). Encysted blebs were seen in 21 (23.9%) patients in the DM group and in 12 (12.4%) patients in the control group (P=0.041). Conclusion. PACG patients with DM without retinopathy undergoing primary trabeculectomy with MMC may have a lower long-term surgical survival rate compared with patients without DM. © 2017 Jian Liu et al.


Li W.,Shandong University | Song A.-P.,Second Peoples Hospital of Jinan | Zhao F.,Cornell University | Hu Y.-M.,Shandong University | Hua M.,Chinese Institute of Scientific and Technical Information
Oncology Reports | Year: 2013

Transforming growth factor (TGF)-β-inducible nuclear protein 1 (TINP1) is a novel gene, which is localized at chromosome 5q13 where frequent abnormalities in hairy cell leukemia (HCL) occur. The present study investigated the effects of TINP1 knockdown or overexpression on the viability and gene expression of various tumor cell lines. siTINP1 was designed to knock down TINP1 expression. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to assess gene expression; the cell counting kit-8 (CCK-8) assay was used to detect cell viability, and luciferase and flow cytometry assays were used to determine gene activity. TINP1 was widely expressed in various cell lines. In addition, TINP1 siRNA was able to knock down TINP1 expression in HeLa cells. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.


Li B.,Second Peoples Hospital of Jinan | Zhang M.,Second Peoples Hospital of Jinan | Liu W.,Second Peoples Hospital of Jinan | Wang J.,Second Peoples Hospital of Jinan
Current Eye Research | Year: 2016

Purpose: To compare the efficacy and safety of two different traction suture (superior rectus traction suture and peripheral lamellar corneal traction suture) in patients undergoing trabeculectomy. Methods: This retrospective trial compared outcomes of 385 eyes that underwent trabeculectomy with superior rectus traction suture (SRTS group; 206 eyes; 53.3%) and peripheral lamellar corneal traction suture (CTS group; 179 eyes; 46.7%). Qualified surgical success is defined as an intraocular pressure (IOP) between 6 and 21 mm Hg with or without topical anti-glaucoma medication. Results: After a follow-up of 24 months, the difference of IOP was significant (p = 0.042). The mean number of anti-glaucoma medications was 3.5 ± 0.6 and 3.5 ± 0.5 preoperatively (p = 1.000), whereas 0.18 ± 0.32 and 0.10 ± 0.39 at 24 months (p = 0.028). The 24-month qualified surgical success rates were 88.3% and 94.4% for both groups (p = 0.035; log-rank test). The difference between the two groups in regard to maximal bleb area is statistically significant (p = 0.011), with CTS group exhibiting more diffuse bleb area. The difference between the two groups in regard to the vascularity at the central and peripheral part of bleb is statistically significant (p = 0.019; p = 0.023, respectively). Encysted blebs was seen in 27 (13.1%) eyes in SRTS group and in 12 (6.7%) eyes in CTS group (p = 0.041). Conclusion: The use of superior rectus traction suture is significantly associated with a lower success rate than corneal traction suture, suggesting that surgeons should use a corneal fixation suture if at all possible. © 2016 Taylor & Francis Group, LLC.


Huang C.,Shandong University | Huang C.,Second Peoples Hospital of Jinan | Fu T.,Second Peoples Hospital of Jinan | Zhang T.,Second Peoples Hospital of Jinan | And 3 more authors.
BMC Ophthalmology | Year: 2013

Background: Scleral buckling surgery and pars plana vitrectomy are competing methods in the treatment of retinal detachment. The recent development of spectral-domain optical coherence tomography (SD-OCT) has dramatically improved the visualization of the photoreceptor layer relative to conventional OCT, and offers new opportunities to investigate the discordances between anatomic and functional outcomes after retinal detachment surgery. Hence, the study aim was to use SD-OCT to compare the postoperative macular recovery between scleral buckling and vitrectomy for macular-off rhegmatogenous retinal detachment. Methods. In this retrospective observational case series, we observed 32 patients who underwent scleral buckling surgery (group 1) and 26 patients who underwent pars plana vitrectomy (group 2) as the primary surgery for macula-off rhegmatogenous retinal detachment. OCT was used to examine microstructural changes in the macular area. Results: The mean visual acuity improvement was 0.4 ± 0.8 logMAR in group 1 and 0.7 ± 0.9 logMAR in group 2. As detected by SD-OCT, subretinal fluid was present in 26 of the group 1 eyes (81.3%) and 5 of the group 2 eyes (19.2%) at 8 weeks postoperatively.This difference was statistically significant (Fisher's exact test, P < 0.05). Moreover, detection by SD-OCT revealed epiretinal membranes in 5 of the group 1 eyes (15.6%) and 11 of the group 2 eyes (42.3%), a difference that was statistically significant (Fisher's exact test, P < 0.05). Conclusions: Macular recovery and the mean visual acuity differed between the 2 groups of patients. With the help of SD-OCT, we observed that subretinal fluids could persist for a relatively longer period after scleral buckling. Based on our results, we conclude that primary vitrectomy surgery is a better choice for macular recovery of the macula-off rhegmatogenous retinal detachment. © 2013 Huang et al.; licensee BioMed Central Ltd.


Zhang Y.,Shandong University | Zhang Y.,Material Evidence Authentication Center | Zou C.,Second Peoples Hospital of Jinan | Liu L.,Material Evidence Authentication Center | And 6 more authors.
Journal of Cataract and Refractive Surgery | Year: 2013

Purpose: To determine the effect of the duration of irradiation on the biomechanical parameters of combined riboflavin-ultraviolet-A (UVA) collagen crosslinking (CXL) in rabbit sclera. Setting: Department of Ophthalmology, Provincial Hospital affiliated with Shandong University, Shandong, China. Design: Experimental study. Methods: Thirty-six New Zealand rabbits were divided into 6 groups based on the duration of irradiation (10, 20, 30, 40, 50, or 60 minutes). After the application of riboflavin 0.1% drops (without dextran) as a photosensitizer, the animals were irradiated with 3 mW/cm2 UVA at 365 nm. Only the left eye of each rabbit was treated. All the animals were humanely killed 24 hours postoperatively. One eye in each treated group was used for light microscopy. The other treated eye and all control eyes were prepared for biomechanical testing. The biomechanical parameters were ultimate stress, Young modulus, and the physiological modulus. Results: The eyes irradiated for 10 or 20 minutes did not differ significantly from the control eyes. Stress-strain measurement of scleral strips irradiated for 40 minutes or longer showed a significant increase in the ultimate stress, Young modulus, and the physiological modulus. There was a significant increase in the physiological modulus of scleral strips irradiated for 30 minutes or longer. Eyes that were irradiated for 50 minutes and 60 minutes had retinal damage. Conclusions: Riboflavin-UVA CXL can lead to a noticeable increase in the biomechanical stiffness of the sclera. The physiological modulus is the most sensitive tool to measure stiffness. In this study, the optimum duration of irradiation was 40 minutes. Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned. © 2013 ASCRS and ESCRS.


Fan C.,Shandong University | Fan C.,Second Peoples Hospital of Jinan | Wang Y.,Second Peoples Hospital of Jinan | Ji Q.,Second Peoples Hospital of Jinan | And 2 more authors.
Current Eye Research | Year: 2014

Purpose: To compare visual outcomes and spectral-domain optical coherence tomography results following treatment with intravitreal ranibizumab (IVR) or IVR combined with intravitreal triamcinolone acetonide (IVTA) for macular edema (ME) secondary to central retinal vein occlusion (CRVO). Methods: This prospective, case-controlled study examined 57 eyes (57 patients) with ME secondary to CRVO, which were treated with IVR (0.5mg, n=30 eyes) or IVR (0.5mg) and IVTA (1mg, n=27eyes) as the initial therapy. Further intravitreal treatment was administered as necessary. Results: All 57 patients completed at least 6 months of follow-up. At baseline, mean (±standard error) best-corrected visual acuity (BCVA) was 45.8±23.2 letters in the IVR group and 47.3±19.3 letters in the IVR+IVTA group (p=0.790). Significant improvement in BCVA over baseline was observed in both groups at all six study visits (IVR group: p=0.0003, 0.0001, 0.0018, 0.0145, 0.0107, 0.005; IVR+IVTA group: p=0.0001, 0.0001, 0.0004, 0.0068, 0.0007, 0.0002), with no significant BCVA differences between groups. Significant reduction in mean central subfield thickness, compared with baseline, was also observed in both groups at all six study visits (IVR group, p=0.0001; IVR+IVTA group, p=0.0001), with no significant difference between groups in the magnitude of macular thickness reduction. The mean number of injections was significantly higher (p=0.0001) in the IVR group (4.23±0.56) than in the IVR+IVTA group (3.42±0.41). Conclusions: Treating ME secondary to CRVO with IVR or IVR+IVTA had similar effects on central macular thickness and BCVA in patients with ME secondary to CRVO over a 6-month follow-up period. The mean number of intravitreal injections was higher in the IVR group than in the IVR+IVTA group. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.


Fan C.,Second Peoples Hospital of Jinan | Ji Q.,Second Peoples Hospital of Jinan | Wang Y.,Second Peoples Hospital of Jinan | Shu X.,Second Peoples Hospital of Jinan | Xie J.,Second Peoples Hospital of Jinan
Journal of Ophthalmology | Year: 2014

Background. To compare visual outcomes and spectral-domain optical coherence tomography results following intravitreal ranibizumab treatment for early and mid-idiopathic choroidal neovascularization (ICNV). Methods. This retrospective, case-controlled study examined 44 patients with ICNV in one eye initially treated with intravitreal ranibizumab (0.5 mg). Further intravitreal treatments were administered as necessary. Patients were divided into two groups according to disease duration, that is, ≤3 months or 3-6 months (early and mid-groups), and the data were compared. Results. All patients completed at least 12 months of follow-up. Significant differences were observed between the groups in best-corrected visual acuity and in central macular thickness (CMT) reduction at all five follow-up visits. At the last follow-up (12 months), 19 early group eyes (79.1%) and 10 mid group eyes (50.0%) had statistically significant visual gains of >15 early treatment diabetic retinopathy study (ETDRS) letters (χ 2 = 4.130, P = 0. 042). The mean number of injections was significantly higher (P = 0. 0 0 0 1) in the mid group (2.53 ± 1. 76) than in the early group (1.22 ± 1.01). Conclusions. Early intravitreal ranibizumab for ICNV can result in better visual prognoses, more obvious decreases in CMT, and fewer injections. © 2014 Chuanfeng Fan et al.


Sheng Y.-J.,Second Peoples Hospital of Jinan | Wang Y.,Second Peoples Hospital of Jinan
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2013

Diabetic macular edema (DME) is a leading cause of vision loss in diabetic patients. It is very important to correctly select a treating approach for DME. At present, the treating methods of DME include retinal laser photocoagulation, application of the glucocorticoid, intravitreous injection of anti-vascular endothelial growth factor (VEGF) drugs, administration of inhibitor of protein kinase C, vitrectomy and combined treatment etc. However, each method has its advantage and disadvantage. Retinal photocoagulation, vitrectomy, intravitreous injection and drug delivery system implantation are invasive treatment methods, and they can not rescue damaged retinal photoreceptors. Therefore, it is recommended that DME should be early diagnosed and effective treatment. The research status at home and abroad and future development trends of DME treatment were summarized. Copyright © 2013 by the Chinese Medical Association.


Zhu C.-B.,Shandong University | Chen L.-L.,Second Peoples Hospital of Jinan | Tian J.-J.,Shandong University | Su L.,Shandong University | And 4 more authors.
Annals of Surgical Oncology | Year: 2012

Background. YKL-40 is a member of the mammalian chitinase-like proteins. Elevated serum YKL-40 levels in patients with gastrointestinal cancer at time of diagnosis are associated with poor prognosis. The aim of this study is to evaluate the prognostic value of serum YKL-40 before surgery and during follow-up in hepatocellular carcinoma (HCC) patients receiving curative resection. Methods. Serum YKL-40 levels were determined by enzyme-linked immunosorbent assay. Overall and recurrence-free survival (RFS) curves were constructed using the Kaplan-Meier method and compared by the log-rank test. A Cox proportional-hazards regression model was performed to identify independent prognostic factors. Median follow-up time was 35 months. Results. Baseline serum YKL-40 was elevated in 56% of patients with HCC receiving curative resection. Patients with elevated serum YKL-40 had significantly shorter overall and RFS than patients with normal serum YKL-40 (P = 0.003 and P = 0.001, respectively). Multivariate Cox regression analyses indicated that baseline serum YKL-40 was an independent prognostic variable for overall and RFS [hazard ratio (HR) = 1.968, 95% confidence interval (CI): 1.093-3.543, P = 0.024; HR = 1.891, 95% CI: 1.106-3.232, P = 0.020; respectively]. After curative resection, high serum YKL-40 (log-transformed continuous variable) within 6 months predicted significantly poorer overall survival (HR = 3.003, 95% CI: 1.323-6.817, P = 0.009). Conclusions. This study indicated that serum YKL-40 was an independent prognostic factor for overall and RFS in HCC patients receiving curative resection. Serial monitoring of serum YKL-40 after curative resection may provide prognostic information. © Society of Surgical Oncology 2011.


PubMed | Second Peoples Hospital of Jinan and Shandong University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2015

Endostatin (ES) is an endogenous angiogenesis inhibitor that has the ability to inhibit tumor growth and metastasis. However, its clinical application is limited by a number of disadvantages, such as poor stability, short half-life and the requirement of high doses to maintain its efficacy. The chemical modification on ES may offer a solution to these disadvantages. The aim of the present study was to evaluate the effects of ES, polysulfated heparin-endostatin (PSH-ES) and polyethylene glycol-endostatin (PEG-ES) on the endothelial cell proliferation and angiogenesis associated with corneal neovascularization (CNV) and to determine their mechanisms of action. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) was used to study the effects of ES and its derivatives on endothelial cell proliferation

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