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Tan B.,University of Sichuan | Tan B.,Sichuan Academy of Medical science | Wei R.-Q.,University of Sichuan | Wei R.-Q.,Second Peoples Hospital of Foshan | And 8 more authors.
Journal of Surgical Research | Year: 2013

Purpose: Acellular porcine small intestinal submucosa (SIS) has been successfully used for esophagoplasty in dogs. However, this has not led to complete epithelialization and muscular regeneration. We undertook the present study to assess the effect of tissue-engineered esophagus generated by seeding bone marrow mesenchymal stem cells (BMSCs) onto an SIS scaffold (BMSCs-SIS) in a canine model. Methods: We cultured, passaged, and measured autologous BMSCs and myoblasts with cell proliferation and immunohistochemical assays. We labeled the third passage of BMSCs with PKH-26, a fluorescent dye, before seeded it onto the SIS. We resected canine cervical esophagus to generate a defect 5 cm in length and 50% in circumference, which we repaired with BMSCs-SIS or SIS alone. Results: Four weeks later, barium esophagram demonstrated that esophageal lumen surface of the patch graft was smoother in the BMSCs-SIS group compared with the SIS group. Histological examination suggested a strong similarity between BMSCs and esophageal myoblasts in terms of morphology and function. Although both BMSCs-SIS and SIS repaired the esophageal defects, we noted complete re-epithelialization with almost no inflammation only in the former group. By 12 wk after the surgery, we observed long bundles of skeletal muscles only in the BMSCs-SIS group, where the microvessel density was also much greater. Conclusions: Bone marrow mesenchymal stem cells on an SIS scaffold can promote re-epithelialization, revascularization, and muscular regeneration. This approach may provide an attractive option for esophageal regeneration.


Wang L.,Second Peoples Hospital of FoShan | Wang L.,PLA Fourth Military Medical University | Huang L.,First Peoples Hospital of GuangZhou | Lin Z.,PLA Fourth Military Medical University | And 2 more authors.
Synthetic Metals | Year: 2011

In this paper, we construct core-shell structured nanoparticles, where magnetic Fe2O3 nanoparticles are used as the inner core and SiO2 doped with phosphorescent Ru(II) complex is used as the outer shell. The obtained magnetic-luminescent composite nanoparticles are identified and characterized by XRD analysis, IR spectrum, field-emission scanning electron microscopy, transmission electron microscopy, and fluorescence image, which confirms the core-shell structure. Then the magnetic and photophysical properties of the composite nanoparticles are investigated in detail. Data suggest that the outer shell of SiO2 weakens the magnetic response of Fe2O3 core by showing a smaller coercivity value compared with that of pure Fe2O3 nanoparticles. The composite nanoparticles are red-emitting ones, and the emission signal is sensitive towards oxygen concentration variations. However, the decreased excited state lifetime and weak emission of the Ru(II) emissive center limit the sensitivity. © 2011 Elsevier B.V. All rights reserved.


Huang D.,Sun Yat Sen University | Luo Q.,Second Peoples Hospital of Foshan | Yang H.,Sun Yat Sen University | Mao Y.,Sun Yat Sen University
Investigative Ophthalmology and Visual Science | Year: 2014

PURPOSE. To explore changes in lacrimal gland and tear inflammatory cytokines in thyroidassociated ophthalmopathy (TAO) patients. METHODS. Patients with TAO were divided into active and inactive TAO groups. These two TAO groups and the control completed the Ocular Surface Disease Index (OSDI), underwent thorough ophthalmologic examinations, and underwent orbital magnetic resonance scan to measure the size of the lacrimal gland. Basal tears, reflex tears induced by nasal stimulation and serum samples, were collected to analyze the concentrations of interleukin (IL)-1β, IL-6, IL-7, IL-17A, interferon γ, and tumor necrosis factor a by multiplex bead analysis. RESULTS. The coronal lacrimal gland area was significantly larger in active (P < 0.000) and inactive TAO (P 1/4 0.002) than in the control, and the axial lacrimal gland area was significantly larger in active (P < 0.000) and inactive TAO (P = 0.001) than in the control. The coronal lacrimal gland width was significantly greater in active (P < 0.000) and inactive TAO (P = 0.001) than in the control, and axial lacrimal gland width was significantly greater in active (P < 0.000) and inactive TAO (P = 0.035) than in the control. In TAO patients, the axial area was positively correlated with IL-1β and IL-17A concentrations in tears (r = 0.357, P = 0.013; r = 0.359, P = 0.012), and both coronal and axial areas were positively correlated with IL-6 concentrations in tears (r = 0.346, P = 0.016; r = 0.340, P = 0.018). CONCLUSIONS. Increased inflammatory cytokines play an important role in ocular surface damages, and might be associated with the inflammatory involvement of the lacrimal gland. © 2014 The Association for Research in Vision and Ophthalmology, Inc.


Yang Y.,Sun Yat Sen University | Yang Y.,Second Peoples Hospital of Foshan | Liu X.,Sun Yat Sen University | Huang J.,Sun Yat Sen University | And 5 more authors.
Molecular Vision | Year: 2012

Purpose: Oxidative stress induced trabecular meshwork cells death is believed to be involved in the pathogenesis and progression of primary open-angle glaucoma (POAG). However, the intrinsic mechanism is yet to be clarified. This study is to investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in tert-butyl hydroperoxide (tBHP)-induced apoptosis of human trabecular meshwork (iHTM) cells. Methods: The human trabecular meshwork cells were treated with tBHP for 1 or 2 h with or without pretreatment of SB203580, an inhibitor of MAP kinase homologs. Cell viability was analyzed using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5- diphenyl-2h-tetrazolium bromide assay. Reactive oxygen species (ROS) levels were determined using dihydrodichlorofluorescein staining, and the chymotrypsin-like protease activities were measured using the Suc-LLVYaminoluciferin substrate. Cell apoptosis was analyzed by Hoechst 33258 staining and annexin V-PI labeling. The protein level of phospho-p38 was measured using western blot analysis. Results: The intracellular ROS increased more than 50 fold and more than 100 fold after tBHP exposure for 1 h and 2 h, respectively (p<0.05). However, there was no difference in ROS levels between SB203580(-) and SB203580(+) cells (p>0.05). In 1 h tBHP treatment group, the cell viability was significantly improved in SB203580(+) cells (81.08%±1.93%) compared to the SB203580(-) cells (69.35%±1.52%), the chymotrypsin-like proteasome inactivation decreased in SB203580(+) cells (60.94%±0.55%) compared to the SB203580(-) cells (70.59%±0.88%), and apoptosis was impoved in SB203580(+) cells (12.75%±1.91%) compared to the SB203580(-) (28.23%±3.23%) (p<0.05). In 2 h tBHP treatment group, cell viability improved in SB203580(+) cells (76.72%±2.11%) compared to SB203580(-) cells (57.88%±2.20%), chymotrypsin-like proteasome inactivation was improved in SB203580(+) cells (62.99%±0.41%) compared to SB203580(-) cells (74.93%±0.54%), and apoptosis was improved in SB203580(+) cells (20.40%±3.44%) compared to SB203580(-) cells (39.20%±5.91%) (p<0.05). Phosphorylation of p38MAPK was significantly increased after tBHP exposure in SB203580 (-) cells and decreased sharply in SB203580(+) cells than that of control group (p<0.05). While there was no difference on the original form of p38MAPK among SB203580(-) and SB203580(+) cells after tBHP exposure and control group (p>0.05). Conclusions: Activation of p38MAPK plays an important role in tBHP-induced apoptosis of iHTM cells. Further study on the mechanisms of p38MAPK in human TM cell apoptosis may help to illuminate the pathogenesis of POAG. © 2012 Molecular Vision.


PubMed | Southern Medical University and Second Peoples Hospital of Foshan
Type: | Journal: Journal of immunological methods | Year: 2016

sDC-SIGN is the soluble form of dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN, CD209), which is a molecule involved with pathogen recognition and immune regulation. However, there is no commercially available ELISA kit for detecting human sDC-SIGN, and the normal range of this molecule is unknown. Here, we describe an ELISA for detecting human sDC-SIGN with high specificity. First, sDC-SIGN protein was expressed and purified. Monoclonal and polyclonal antibodies were then raised against the purified protein and subsequently characterized. A sandwich ELISA was developed using polyclonal antibodies specific for sDC-SIGN for capture and a biotin-labeled monoclonal antibody specific for sDC-SIGN for detection of protein. This method has sensitivity up to 0.2 ng/ml. Using this ELISA, we found that the concentration of sDC-SIGN in sera of healthy volunteers ranges from 0-319 ng/ml with a mean concentration of 27.14 ng/ml. Interestingly, the concentration of sDC-SIGN in sera from patients with cancer or chronic hepatitis B virus (CHB) infection was lower than that of health controls. The mean concentrations of sDC-SIGN in cancer patients and chronic hepatitis B virus infection patients were 3.2 ng/ml and 3.8 ng/ml, respectively. We developed a sandwich ELISA for detecting human sDC-SIGN and demonstrated its use by assessing sera concentrations of sDC-SIGN in patients with cancer and chronic CHB infection compared to that of healthy controls.


Shi Y.,Jinan University | Lv H.,Second Peoples Hospital of Foshan | Fu Y.,Jinan University | Lu Q.,Capital Medical University | And 4 more authors.
Biomedical Materials (Bristol) | Year: 2013

A soft and biocompatible hydrogel exhibiting a higher loading and the sustained release of gatifloxacin (GFLX) was developed as the potential matrix to fabricate a therapeutic contact lens for curing bacterial keratitis. 2-hydroxyethyl methacrylate (HEMA) and five other kinds of vinyl monomers with different side groups were used as co-monomers. Copolymerization took place in a cornea shaped mould via the gradient temperature-elevating method. The results of drug loading and in vitro release experiments showed that P(HEMA-co-MAA) achieved the highest drug loading of 11.78±0.77 μg mg-1 among the obtained hydrogels, as well as a slow release. In addition, its physical properties and cytocompatibility were also proved suitable and safe for wearing on the eye surface. In animal experiments, a rat model of bacterial keratitis was established and employed to evaluate the clinical results of certain treatments employing obtained hydrogels; saline and GFLX eye drops were used as negative and positive controls, respectively. Corneal abscess and opacity caused by epithelial erosion and stromal ulceration were almost healed after wearing the drug loaded P(HEMA-co-MAA) hydrogel for 48 h. Its excellent antibacterial effect was also confirmed by testing the bacterial activity in tear extraction via the streak line method. © 2013 IOP Publishing Ltd.


Huang H.,Guangdong Medical College | Song L.,Guangdong Medical College | Zhang H.,Guangdong Medical College | Zhang J.,Second Peoples Hospital of Foshan | Zhao W.,Guangdong Medical College
Kidney and Blood Pressure Research | Year: 2014

Background/Aims: An increasing body of evidence demonstrates that L-carnitine plays a pivotal role in lipid metabolism of hemodialysis (HD) patients. However, there are still some reservations about its benefits. Therefore, we performed a meta-analysis to assess the effects of L-carnitine supplementation on lipid profile in HD patients. Methods: Literature search was performed to identify the relevant randomized controlled trials that investigated the effects of L-carnitine on the lipid profile of subjects. Two independent authors used an Excel file to extract data and assess trials quality. The primary effect measure was the difference in means of the final lipid measurements between the intervention and control groups. The meta-analysis was performed with the fixed-effects model or random-effects model according to heterogeneity. Results: Twelve studies with a total of 391 patients met the inclusion criteria. The use of L-carnitine was not associated with a reduction in the total cholesterol (SMD, -0.11; 95% CI, -0.31 to 0.09), HDL-cholesterol (SMD, 0.01; 95% CI, -0.36 to 0.39), VLDL-cholesterol (SMD, 0.54; 95% CI, -0.06 to 1.14), and the serum triglycerides (SMD, -0.12; 95% CI, -0.36 to 0.12). However, L-carnitine can significantly decrease the LDL-cholesterol (SMD, -0.29; 95% CI, -0.53 to -0.06) in HD patients. In a subgroup meta-analysis, a significant LDL-cholesterol-lowering effect of L-carnitine supplementation was observed in intravenous application group, and patients with longer interventional duration and renal diseases. Conclusion: The limited evidence suggests that there was no effect of L-carnitine on serum total cholesterol, HDL-cholesterol, VLDL-cholesterol and serum triglycerides. By contrast, this meta-analysis suggests a promising effect of L-carnitine on LDL-cholesterol. Further large-scale, well-designed randomized controlled trials are urgently needed © 2014 S. Karger AG, Basel.


Shi Y.,Jinan University | Lv H.,Second Peoples Hospital of Foshan | Lu X.,Jinan University | Huang Y.,Jianze Pharmaceutical Company Ltd | And 2 more authors.
Journal of Materials Chemistry | Year: 2012

Herein we have combined the molecular imprinting technique and thermally-initiated precipitation polymerization to develop novel molecularly imprinted poly(methacrylic acid) nanospheres (PMAA MIPNs) for sustained release of gatifloxacin (GFLX). In the process, ethylene glycol dimethacrylate (EGDMA) and GFLX were used as the cross-linker and template, respectively. The effects of reaction medium and concentration of the monomers such as MAA, EGDMA and GFLX were systematically investigated. Uniform nanospheres with diameter equal to 136.0 ± 8.1 nm and particle dispersion index (PDI) equal to 1.01 were obtained when the total monomer concentration was 1 vol%, the EGDMA content was 70 mol% and the GFLX/MAA ratio was 1:15 (mol/mol). The drug loading profiles and in vitro release studies indicated that PMAA MIPNs are better drug delivery vessels and have an improved release profile than non-imprinted ones, which was attributed to the reinforced interaction between GFLX and PMMA side groups due to the specific GFLX molecular arrangement in the PMAA polymeric matrix. Based on these results, these novel PMAA MIPNs are a promising candidate for sustained release of GFLX. © 2012 The Royal Society of Chemistry.


Lu X.-F.,Jinan University | Shi Y.-F.,Jinan University | Lv H.-L.,Second Peoples Hospital of Foshan | Fu Y.-Y.,Jinan University | And 2 more authors.
Journal of Materials Science: Materials in Medicine | Year: 2014

Molecularly imprinted poly(hydroxyethyl methacrylate) microspheres (PHEMA MIPMs) were prepared via precipitation polymerization in this article, using gatifloxacin (GFLX), hydroxyethyl methacrylate (HEMA), and ethylene glycol dimethacrylate (EGDMA) as template molecule, functional monomer and cross-linker, respectively. The effects of reaction medium, initial total monomers, cross-linker and molecular imprinting on the polymerization were investigated systematically. The interaction between GFLX and HEMA in pre-solution was studied by UV-Visible spectrophotometer, both size and morphology of products were characterized by a scanning electron microscope. When the total initial monomer concentration was 1 vol%, EGDMA content was 70 mol%, a group of uniform PHEMA MIPMs were prepared at different GFLX/MAA molar ratios, with diameter range from 2.06 ± 0.07 to 2.82 ± 0.20 μm. The results of drug loading and in vitro release experiments demonstrated that PHEMA MIPMs could achieve a higher GFLX loading content and a more acceptable sustained release than non-imprinted ones. © 2014 Springer Science+Business Media.


Zhang X.-X.,Second Peoples Hospital of Foshan
International Journal of Ophthalmology | Year: 2010

• AIM: To explore the surgery effect of the upper eyelid blepharoplasty combined with the levator aponeurosis plication in mild blepharoptosis. • METHODS: We performed upper eyelid blepharoplasty in 326 patients 652 eyes and combined with the levator aponeurosis plication in 36 patients 65 eyes. • RESULTS: Followed up for 1-6 months, the double eyelid was symmetry and showed good shape. The palpebral fissure was enlarged 1.5mm in average. The mild notch deformity of margin was in 2 eyes, and disappered after 3 months by massaging upper eyelid. Under-corrected was in 1 eye. There was lagohthalmus 1-3mm early after operation, average 2.1mm, 1 month later lagohthalmus disappered and no exposure keratitis occurred. • CONCLUSION: The upper eyelid blepharoplasty combined with the levator aponeurosis plication in mild blepharoptosis patient can achieve a high success rate and high satisfactory rate.

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