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Huang D.,Sun Yat Sen University | Luo Q.,Second Peoples Hospital of Foshan | Yang H.,Sun Yat Sen University | Mao Y.,Sun Yat Sen University
Investigative Ophthalmology and Visual Science | Year: 2014

PURPOSE. To explore changes in lacrimal gland and tear inflammatory cytokines in thyroidassociated ophthalmopathy (TAO) patients. METHODS. Patients with TAO were divided into active and inactive TAO groups. These two TAO groups and the control completed the Ocular Surface Disease Index (OSDI), underwent thorough ophthalmologic examinations, and underwent orbital magnetic resonance scan to measure the size of the lacrimal gland. Basal tears, reflex tears induced by nasal stimulation and serum samples, were collected to analyze the concentrations of interleukin (IL)-1β, IL-6, IL-7, IL-17A, interferon γ, and tumor necrosis factor a by multiplex bead analysis. RESULTS. The coronal lacrimal gland area was significantly larger in active (P < 0.000) and inactive TAO (P 1/4 0.002) than in the control, and the axial lacrimal gland area was significantly larger in active (P < 0.000) and inactive TAO (P = 0.001) than in the control. The coronal lacrimal gland width was significantly greater in active (P < 0.000) and inactive TAO (P = 0.001) than in the control, and axial lacrimal gland width was significantly greater in active (P < 0.000) and inactive TAO (P = 0.035) than in the control. In TAO patients, the axial area was positively correlated with IL-1β and IL-17A concentrations in tears (r = 0.357, P = 0.013; r = 0.359, P = 0.012), and both coronal and axial areas were positively correlated with IL-6 concentrations in tears (r = 0.346, P = 0.016; r = 0.340, P = 0.018). CONCLUSIONS. Increased inflammatory cytokines play an important role in ocular surface damages, and might be associated with the inflammatory involvement of the lacrimal gland. © 2014 The Association for Research in Vision and Ophthalmology, Inc.

Shi Y.,Jinan University | Lv H.,Second Peoples Hospital of Foshan | Lu X.,Jinan University | Huang Y.,Jianze Pharmaceutical Company Ltd | And 2 more authors.
Journal of Materials Chemistry | Year: 2012

Herein we have combined the molecular imprinting technique and thermally-initiated precipitation polymerization to develop novel molecularly imprinted poly(methacrylic acid) nanospheres (PMAA MIPNs) for sustained release of gatifloxacin (GFLX). In the process, ethylene glycol dimethacrylate (EGDMA) and GFLX were used as the cross-linker and template, respectively. The effects of reaction medium and concentration of the monomers such as MAA, EGDMA and GFLX were systematically investigated. Uniform nanospheres with diameter equal to 136.0 ± 8.1 nm and particle dispersion index (PDI) equal to 1.01 were obtained when the total monomer concentration was 1 vol%, the EGDMA content was 70 mol% and the GFLX/MAA ratio was 1:15 (mol/mol). The drug loading profiles and in vitro release studies indicated that PMAA MIPNs are better drug delivery vessels and have an improved release profile than non-imprinted ones, which was attributed to the reinforced interaction between GFLX and PMMA side groups due to the specific GFLX molecular arrangement in the PMAA polymeric matrix. Based on these results, these novel PMAA MIPNs are a promising candidate for sustained release of GFLX. © 2012 The Royal Society of Chemistry.

Shi Y.,Jinan University | Fu Y.,Jinan University | Lv H.,Second Peoples Hospital of Foshan | Zhong J.,Jinan University | And 3 more authors.
Materials Letters | Year: 2014

A novel poly(methacrylic acid) (PMAA) microcarriers coated with nanolayered chitosan(Cs) and carboxyl modified multi-walled carbon nanotubes (MWCNTs-COOH) via self-assembly method, has been successfully prepared for better loading and sustained release of gatifloxacin (GFLX). Due to the multi-loading and gradient release properties, PMAA/Cs/CNTs could adsorb a great amount of drug (84.89±9.63 μg mg-1), and 64.74±2.78% of its loading could slowly release in 24 h, which is important to delivery GFLX effectively and to reduce the risk of side effects. © 2014 Elsevier B.V.

Shi Y.,Jinan University | Lv H.,Second Peoples Hospital of Foshan | Fu Y.,Jinan University | Lu Q.,Capital Medical University | And 4 more authors.
Biomedical Materials (Bristol) | Year: 2013

A soft and biocompatible hydrogel exhibiting a higher loading and the sustained release of gatifloxacin (GFLX) was developed as the potential matrix to fabricate a therapeutic contact lens for curing bacterial keratitis. 2-hydroxyethyl methacrylate (HEMA) and five other kinds of vinyl monomers with different side groups were used as co-monomers. Copolymerization took place in a cornea shaped mould via the gradient temperature-elevating method. The results of drug loading and in vitro release experiments showed that P(HEMA-co-MAA) achieved the highest drug loading of 11.78±0.77 μg mg-1 among the obtained hydrogels, as well as a slow release. In addition, its physical properties and cytocompatibility were also proved suitable and safe for wearing on the eye surface. In animal experiments, a rat model of bacterial keratitis was established and employed to evaluate the clinical results of certain treatments employing obtained hydrogels; saline and GFLX eye drops were used as negative and positive controls, respectively. Corneal abscess and opacity caused by epithelial erosion and stromal ulceration were almost healed after wearing the drug loaded P(HEMA-co-MAA) hydrogel for 48 h. Its excellent antibacterial effect was also confirmed by testing the bacterial activity in tear extraction via the streak line method. © 2013 IOP Publishing Ltd.

Lu X.-F.,Jinan University | Shi Y.-F.,Jinan University | Lv H.-L.,Second Peoples Hospital of Foshan | Fu Y.-Y.,Jinan University | And 2 more authors.
Journal of Materials Science: Materials in Medicine | Year: 2014

Molecularly imprinted poly(hydroxyethyl methacrylate) microspheres (PHEMA MIPMs) were prepared via precipitation polymerization in this article, using gatifloxacin (GFLX), hydroxyethyl methacrylate (HEMA), and ethylene glycol dimethacrylate (EGDMA) as template molecule, functional monomer and cross-linker, respectively. The effects of reaction medium, initial total monomers, cross-linker and molecular imprinting on the polymerization were investigated systematically. The interaction between GFLX and HEMA in pre-solution was studied by UV-Visible spectrophotometer, both size and morphology of products were characterized by a scanning electron microscope. When the total initial monomer concentration was 1 vol%, EGDMA content was 70 mol%, a group of uniform PHEMA MIPMs were prepared at different GFLX/MAA molar ratios, with diameter range from 2.06 ± 0.07 to 2.82 ± 0.20 μm. The results of drug loading and in vitro release experiments demonstrated that PHEMA MIPMs could achieve a higher GFLX loading content and a more acceptable sustained release than non-imprinted ones. © 2014 Springer Science+Business Media.

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