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Wang B.,University of Sichuan | Wang B.,Second Peoples Hospital of Chengdu | He P.,Third Peoples Hospital of Chengdu | Dong B.,University of Sichuan
Archives of Gerontology and Geriatrics | Year: 2015

Objective: To explore the associations between social networks, social contacts, and cognitive impairment in the very elderly aged 90-108 years. Methods: Data were from subjects of the Project of Longevity and Ageing in Dujiangyan, China. The socio-demographic, social networks, and social contacts data were collected and cognitive function was assessed in all subjects using the Mini-Mental State Examination (MMSE). Results: 764 Chinese nonagenarians and centenarians (67.41% women, mean age 93.47 years) were included. The mean MMSE score was 14.99. ±. 5.93). The prevalence of cognitive impairment was 64.53%. The mean social contact score was 4.37. ±. 1.86. There were significantly different cognitive function scores among individuals with different marital status, number of close friends, and different social contact levels (all P<. 0.05). Spearman rank correlation analysis showed that number of close friends and social contact scores were significantly positively but single status was significantly negatively correlated with the MMSE scores (all P<. 0.05). Multiple logistic regression analysis showed that there were associations between single status, no close friend, and low level of social contact with cognitive impairment (all P<. 0.05), but not other social network variables. Conclusion: Single status, no close friend, and low level of social contact were associated with increased risk of cognitive impairment in Chinese nonagenarians and centenarians. Our finding might add new information for social networks, social contacts, and cognitive research in the elderly. © 2015 Elsevier Ireland Ltd.

Wang B.,University of Sichuan | Wang B.,Second Peoples Hospital of Chengdu | He P.,Third Peoples Hospital of Chengdu | Dong B.,University of Sichuan
Geriatrics and Gerontology International | Year: 2015

Aim: We explored the association between family functioning and cognitive impairment in the very elderly aged 90-108years. Methods: The present study comprised data from subjects included in the 2005 Project of Longevity and Aging in Dujiangyan, China. Sociodemographic and family functioning data were collected, and cognitive function was assessed in all subjects using the Mini-Mental State Examination. Results: Data from 699 Chinese nonagenarians and centenarians were included. The prevalence of cognitive impairment was 62.8%. The prevalence of family dysfunction was 52.2%, including 8.6% severe and 43.6% moderate dysfunction. There were significant differences among individuals with different family functioning level with regard to cognitive function scores (P=0.005) or cognitive impairment prevalence (P=0.012). Subjects with cognitive impairment had lower family functioning scores than those without cognitive impairment (P=0.004). Pearson's correlation analysis showed that family functioning scores were correlated with Mini-Mental State Examination scores (r=0.13, P=0.001). Multiple logistic regressions showed that severe family dysfunction was a risk factor for cognitive impairment. The effect remained after adjusting for sociodemographic status, life habits and metabolic indicators. Conclusions: Family functioning was related to cognitive impairment among Chinese nonagenarians and centenarians. We found that the higher the family functioning scores, the higher the Mini-Mental State Examination scores. Severe family dysfunction was associated with increased risk of cognitive impairment. © 2015 Japan Geriatrics Society.

Wang T.,Second Peoples Hospital of Chengdu | Liu H.,Sun Yat Sen University | Zheng Z.,Sun Yat Sen University | Li Z.,Sun Yat Sen University | And 3 more authors.
Spine | Year: 2013

Study Design.: An in vitro biomechanical study of 3 lumbosacral fixation techniques in human cadaveric lumbar-pelvic spine models. Objective.: To compare the in vitro biomechanical effect of a novel 4-rod lumbosacral reconstruction technique with conventional techniques in a human cadaveric lumbopelvic model, and to evaluate the benefit of adding supplementary rod fixation. Summary of Background Data.: Spinopelvic fixation involving the sacrum remains a difficult clinical challenge. Numerous lumbopelvic reconstruction methods based on the Galveston 2-rod technique have been proposed. Recently, a novel technique using supporting longitudinal rods across the lumbopelvic junction was reported. However, no comparative in vitro biomechanical testing was performed to evaluate the benefit of adding supplementary fixation at the L5-S1 levels. Methods.: Seven fresh-frozen cadaveric lumbar-pelvic spines were prepared and tested for bone mineral density. The intact cadavers underwent a flexibility test, followed by insertion of the instrumented construct. Three constructs were tested: S1 screws alone (group 1), S1 screws plus iliac screws (group 2), and the 4-rod technique (group 3). Rotational angles of the L1-S1 and L5-S1 segments were measured to study the stability of the 3 lumbosacral fixation constructs compared with the intact spine. Nondestructive, multidirectional flexibility tests that included 4 loading methods followed by a destructive flexural load to failure were performed using an material testing machine. The lumbosacral peak range of motion (ROM) (millimeters or degrees) and ultimate failure load (Nm) of the 3 reconstruction techniques were statistically compared using a 1-way analysis of variance combined with a Student-Newman-Keuls post hoc test. Results.: The average bone mineral density of the 7 specimens was 0.81 ± 0.09 g/cm. The ROM of the 3 fixation constructs was significantly smaller than that of the intact group in all 6 directions (P < 0.05). In lateral bending, the ROM of groups 2 and 3 was significantly smaller than that of group 1 (P < 0.05), but groups 2 and 3 were not significantly different from each other (P > 0.05). In flexion-extension, the ROM of groups 1 and 3 was significantly smaller than group 2 (P < 0.05), but groups 1 and 3 were not significantly different from each other (P > 0.05). In axial rotation, the ROM of group 3 was significantly smaller than those of groups 1 and 2 (P < 0.05), but groups 1 and 2 were not significantly different from each other (P > 0.05). Conclusion.: The 4-rod technique achieved stable biomechanical effects in lumbosacral fixation. At the L5-S1 junction, the 4-rod technique demonstrated better stability than the constructs using S1 screws or S1 screws plus iliac screws. Copyright © 2013 Lippincott Williams & Wilkins.

Wang C.,General Hospital of Chengdu Army | Wang M.,Second Peoples Hospital of Chengdu | Liu Y.,General Hospital of Chengdu Army | Zeng P.,General Hospital of Chengdu Army
International Immunopharmacology | Year: 2011

Increasing evidence demonstrates that pathological B cells play an essential role in the triggering and development of human systemic lupus erythematosus (SLE). A rational strategy for treating SLE might be to delete B cells thereby suppressing autoimmunity. Commercial monoclonal anti-CD20 antibody is widely used for treatment of B cell-related autoimmune disorders. However its long term use is limited by several factors including short half-life, high cost, and possible side effects of antibody protein therapy. Therefore, we constructed a recombinant adenovirus encoding the murine anti-CD20 antibody gene, and used it to immunize lupus-prone (BWF1) mice. Our data demonstrated that administration of adenovirus encoding the murine anti-CD20 antibody gene generated murine anti-CD20 antibody, which resulted in elimination of B cells in BWF1 mice. In addition, the anti-CD20 reduced serum anti-dsDNA antibody levels, impeded the development of proteinuria and improved the survival of BWF1 mice. These findings suggested that the adenovirus encoding murine anti-CD20 antibody gene might provide an alternative strategy for B cell-mediated diseases. © 2011 Elsevier B.V. All rights reserved.

Chen L.,North Sichuan Medical College | Jiang K.,North Sichuan Medical College | Jiang H.,Second Peoples Hospital of Chengdu | Wei P.,North Sichuan Medical College
Experimental and Therapeutic Medicine | Year: 2014

Frequent acquisition of drug resistance is often associated with the chemotherapy of malignant tumors, including osteosarcoma. A number of studies have demonstrated a critical role for autophagy in osteosarcoma development, therapy and drug resistance. However, the molecular mechanisms underlying the autophagy-mediated chemotherapy resistance of osteosarcoma cells remain largely unknown. In the present study, we determined the autophagy and microRNA-155 (miR-155) expression induced by chemotherapeutic drugs in osteosarcoma cells. Then we determined the promotory role of miR-155 to the chemotherapy-induced autophagy. Our results demonstrated that microRNA-155 (miR-155) expression was highly induced during chemotherapy of osteosarcoma cells, and this was accompanied by upregulated autophagy. The increased miR-155 expression levels upregulated anticancer drug-induced autophagy in osteosarcoma cells and ameliorated the anticancer drug-induced cell proliferation and viability decrease. Therefore, the results of the present study demonstrated that miR-155 mediated drug-resistance in osteosarcoma cells by inducing autophagy. The present study recognized a novel mechanism of chemoresistance in osteosarcoma cancers.

Ling X.,Chongqing Medical University | Ling X.,Second Peoples Hospital of Chengdu | Linglong P.,Chongqing Medical University | Weixia D.,Chongqing Medical University | Hong W.,Chongqing Medical University
PLoS ONE | Year: 2016

Zonulin protein is a newly discovered modulator which modulates the permeability of the intestinal epithelial barrier by disassembling intercellular tight junctions (TJ). Disruption of TJ is associated with neonatal necrotizing enterocolitis (NEC). It has been shown bifidobacterium could protect the intestinal barrier function and prophylactical administration of bifidobacterium has beneficial effects in NEC patients and animals. However, it is still unknown whether the zonulin is involved in the gut barrier dysfunction of NEC, and the protective mechanisms of bifidobacterium on intestinal barrier function are also not well understood. The present study aims to investigate the effects of bifidobacterium on intestinal barrier function, zonulin regulation, and TJ integrity both in LPS-induced enterocyte barrier injury of Caco-2 monolayers and in a rat NEC model. Our results showed bifidobacterium markedly attenuated the decrease in transepithelial electrical resistance and the increase in paracellular permeability in the Caco-2 monolayers treated with LPS (P < 0.01). Compared with the LPS group, bifidobacterium significantly decreased the production of IL-6 and TNF-α (P < 0.01) and suppressed zonulin release (P < 0.05). In addition, bifidobacterium pretreatment up-regulated occludin, claudin-3 and ZO-1 expression (P < 0.01) and also preserved these proteins localization at TJ compared with the LPS group. In the in vivo study, bifidobacterium decreased the incidence of NEC from 88 to 47% (P < 0.05) and reduced the severity in the NEC model. Increased levels of IL-6 and TNF-α in the ileum of NEC rats were normalized in bifidobacterium treated rats (P < 0.05). Moreover, administration of bifidobacterium attenuated the increase in intestinal permeability (P < 0.01), decreased the levels of serum zonulin (P < 0.05), normalized the expression and localization of TJ proteins in the ileum compared with animals with NEC. We concluded that bifidobacterium may protect against intestinal barrier dysfunction both in vitro and in NEC. This protective effect is associated with inhibition of proinflammatory cytokine secretion, suppression of zonulin protein release and improvement of intestinal TJ integrity. © 2016 Ling et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Wei D.,University of Pittsburgh | Wei D.,Second Peoples Hospital of Chengdu | Li J.,University of Pittsburgh | Li J.,University of Sichuan | And 13 more authors.
Diabetes | Year: 2010

OBJECTIVE - To evaluate the direct impact of n-3 polyunsaturated fatty acids (n-3 PUFAs) on the functions and viability of pancreatic β-cells. RESEARCH DESIGN AND METHODS - We developed an mfat-1 transgenic mouse model in which endogenous production of n-3 PUFAs was achieved through overexpressing a C. elegans n-3 fatty acid desaturase gene, mfat-1. The islets and INS-1 cells expressing mfat-1 were analyzed for insulin secretion and viability in response to cytokine treatment. RESULTS - The transgenic islets contained much higher levels of n-3 PUFAs and lower levels of n-6 PUFAs than the wild type. Insulin secretion stimulated by glucose, amino acids, and glucagon-like peptide-1 (GLP-1) was significantly elevated in the transgenic islets. When challenged with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and γ-interferon (IFN-γ), the transgenic islets completely resisted cytokine-induced cell death. Adenoviral transduction of mfat-1 gene in wild-type islets and in INS-1 cells led to acute changes in the cellular levels of n-3- and n-6 PUFAs and recapitulated the results in the transgenic islets. The expression of mfat-1 led to decreased production of prostaglandin E2 (PGE2), which in turn contributed to the elevation of insulin secretion. We further found that cytokine-induced activation of NF-κB and extracellular signal-related kinase 1/2 (ERK 1/2) was significantly attenuated and that the expression of pancreatic duodenal hemeobox-1 (PDX-1), glucokinase, and insulin-1 was increased as a result of n-3 PUFA production. CONCLUSIONS - Stable cellular production of n-3 PUFAs via mfat-1 can enhance insulin secretion and confers strong resistance to cytokine-induced β-cell destruction. The utility of mfat-1 gene in deterring type 1 diabetes should be further explored in vivo. © 2010 by the American Diabetes Association.

Yang J.,Second Peoples Hospital of Chengdu | Zheng M.,Chongqing Medical University | Chen S.,Chongqing Medical University | Ou S.,Chongqing Medical University | And 4 more authors.
PLoS ONE | Year: 2014

Conclusions: In this population-based survey the community residents have poor awareness of thrombolytic therapy for acute ischemic stroke.Background and Purpose: Thrombolytic therapy rate for acute ischemic stroke remains low, and improving public awareness of thrombolytic therapy may be helpful to reduce delay and increase chances of thrombolytic therapy. Our purpose was to survey the level of knowledge about thrombolytic therapy for acute ischemic stroke among community residents in Yuzhong district, Chongqing, China.Methods: In 2011, a population-based face-to-face interview survey was conducted in Yuzhong district, Chongqing. A total of 1500 potential participants aged ≥18 years old were selected using a multi-stage sampling method.Results: A total of 1101 participants completed the survey. Only 23.3% (95% CI = 20.8 to 25.8) were aware of thrombolytic therapy for acute ischemic stroke, of whom 59.9% (95% CI = 53.9 to 65.9) knew the time window. Awareness of thrombolytic therapy was higher among young people, those with higher levels of education and household income, those with health insurance, and those who knew all 5 stroke warning signs, while awareness of the time window was higher among those aged 75 years or older. Multivariate logistic regression analysis showed that awareness of thrombolytic therapy was independently associated with age, education level, health insurance and knowledge of stroke warning signs (P<0.05). © 2014 PLOS ONE.

Huang G.,Second Peoples Hospital of Chengdu | Zhao J.-L.,Second Peoples Hospital of Chengdu | Du H.,Chongqing Medical University | Lan X.-B.,Chongqing Medical University | Yin Y.-H.,Chongqing Medical University
Circulation Journal | Year: 2010

Background: The aim of the present study was to explore the association of 3 coronary scores with major adverse cardiovascular events (MACE) in patients with acute coronary syndrome (ACS). Methods and Results: The 958 consecutive patients with ACS were followed up until either MACE or 31st December 2008 occurred; 257 patients reached clinical endpoints. Cox regression analysis demonstrated that the Gensini score was associated with 90-day MACE (relative risk (RR) 1.021, P=0.004), 6-month MACE (RR 1.021, P<0.001), 1-year MACE (RR 1.017, P=0.002), and MACE during follow-up (RR 1.010, P=0.040). Leaman score was associated with 90-day MACE (RR 1.094, P=0.014), 6-month MACE (RR 1.098, P=0.002), and 1-year MACE (RR 1.074, P=0.009). The logistic regression analysis demonstrated that the Gensini score (odds ratio (OR) 1.037, P=0.001), Leaman score (OR 1.165, P=0.007) and American College of Cardiology/American Heart Association (ACC/AHA) score (OR 1.235, P=0.025) were all associated with cardiogenic death. Conclusions: The Gensini score provides more valuable prognostic information on cardiovascular risk than either the Leaman or ACC/AHA score in patients with ACS.

PubMed | Second Peoples Hospital of Chengdu and Central South University
Type: Journal Article | Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences | Year: 2017

To isolate bone marrow mesenchymal stem cells (BM-MSCs) and establish the model of chronic kidney disease (CKD) of Wuzhishan (WZS) mini-pig, and to study the repairment effect of BM-MSCs on CKD-induced renal fibrosis in vitro. Methods: Density gradient method was used to isolate and culture BM-MSCs. The cells were verified by morphology, phenotype, differentiation and so on. The left partial ureteral obstruction (LPUUO) was used to establish the CKD model, which was evaluated by B-ultrasound, single-photon emission computed tomography (SPECT), HE and Masson staining. The cells were divided into 3 groups, the tissue plus BM-MSCs group, the tissue group, and the BM-MSCs group, respectively. Seven days later, the supernatants were collected to observe the changes of hepatocyte growth factor (HGF) cumulative release. HE and Masson staining was used to observe the changes of renal tissue. Results: The isolated BM-MSCs possessed the features as follow: fibroblast-like adherent growth; positive in CD29 and CD90 expression while negative in CD45 expression; osteogenic induction and alizarin red staining were positive; alcian blue staining were positive after chondrogenic induction. Twelve weeks after the operation of LPUUO, B-ultrasound showed the thin renal cortical with pelvis effusion; SPETCT showed the left kidney delayed filling and renal impairment. The accumulation of HGF in the tissue plus BM-MSCs group was significantly higher than that in the tissue alone group at the 1st, 5th, 6th, 7th day, respectively (P<0.05). HE staining showed the different degree of renal lesions between the tissue plus BM-MSCs+CKD group and the tissue alone group, which was aggravated with the time going. Masson staining showed that the cumulative optical density of blue-stained collagen fibers in tissue plus BM-MSCs group was significantly lower than that in the tissue group at the 5th to 7th day (P<0.05). Conclusion: BM-MSCs from WZS mini-pig can inhibit or delay the progress of CKD-induced renal fibrosis through autocrine HGF in vitro.

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