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Wang M.,Second Peoples Hospital of Chengdu
International Immunopharmacology | Year: 2011

Increasing evidence demonstrates that pathological B cells play an essential role in the triggering and development of human systemic lupus erythematosus (SLE). A rational strategy for treating SLE might be to delete B cells thereby suppressing autoimmunity. Commercial monoclonal anti-CD20 antibody is widely used for treatment of B cell-related autoimmune disorders. However its long term use is limited by several factors including short half-life, high cost, and possible side effects of antibody protein therapy. Therefore, we constructed a recombinant adenovirus encoding the murine anti-CD20 antibody gene, and used it to immunize lupus-prone (BWF1) mice. Our data demonstrated that administration of adenovirus encoding the murine anti-CD20 antibody gene generated murine anti-CD20 antibody, which resulted in elimination of B cells in BWF1 mice. In addition, the anti-CD20 reduced serum anti-dsDNA antibody levels, impeded the development of proteinuria and improved the survival of BWF1 mice. These findings suggested that the adenovirus encoding murine anti-CD20 antibody gene might provide an alternative strategy for B cell-mediated diseases. © 2011 Elsevier B.V. All rights reserved.

Huang G.,University of Hong Kong | Yeung C.-Y.,University of Hong Kong | Lee K.K.,University of Hong Kong | Liu J.,Second Peoples Hospital of Chengdu | And 6 more authors.
Journal of Oncology | Year: 2014

Background. Androgen deprivation therapy (ADT) in nonmetastatic prostate cancer is unclear. Recent data suggests possible increase in the cardiovascular risks receiving ADT. The aim of the study was to investigate the cardiovascular outcomes in a cohort of Chinese nonmetastatic prostate cancer patients with no previously documented cardiovascular disease. Methods and Results. 745 patients with no previously documented cardiovascular disease and/or diabetes mellitus diagnosed to have nonmetastatic prostate cancer were recruited. Of these, 517 patients received ADT and the remaining 228 did not. After a mean follow-up of 5.3 years, 60 patients developed primary composite endpoint including (1) coronary artery disease, (2) congestive heart failure, and (3) ischemic stroke. Higher proportion of patients on ADT (51 patients, 9.9%) developed composite endpoint compared with those not on ADT (9 patients, 3.9%) with hazard ratio (HR) of 2.06 (95% confidence interval (CI): 1.03-3.24, P = 0.04). Furthermore, Cox regression analysis revealed that only the use of ADT (HR: 2.1, 95% CI: 1.03-4.25, P = 0.04) and hypertension (HR: 2.0, 95% CI: 1.21-3.33, P < 0.01) were independent predictors for primary composite endpoint. Conclusion. ADT in Chinese patients with nonmetastatic prostate cancer with no previously documented cardiovascular disease was associated with subsequent development of cardiovascular events. © 2014 Gang Huang et al.

Wang T.,Second Peoples Hospital of Chengdu | Liu H.,Sun Yat Sen University | Zheng Z.,Sun Yat Sen University | Li Z.,Sun Yat Sen University | And 3 more authors.
Spine | Year: 2013

Study Design.: An in vitro biomechanical study of 3 lumbosacral fixation techniques in human cadaveric lumbar-pelvic spine models. Objective.: To compare the in vitro biomechanical effect of a novel 4-rod lumbosacral reconstruction technique with conventional techniques in a human cadaveric lumbopelvic model, and to evaluate the benefit of adding supplementary rod fixation. Summary of Background Data.: Spinopelvic fixation involving the sacrum remains a difficult clinical challenge. Numerous lumbopelvic reconstruction methods based on the Galveston 2-rod technique have been proposed. Recently, a novel technique using supporting longitudinal rods across the lumbopelvic junction was reported. However, no comparative in vitro biomechanical testing was performed to evaluate the benefit of adding supplementary fixation at the L5-S1 levels. Methods.: Seven fresh-frozen cadaveric lumbar-pelvic spines were prepared and tested for bone mineral density. The intact cadavers underwent a flexibility test, followed by insertion of the instrumented construct. Three constructs were tested: S1 screws alone (group 1), S1 screws plus iliac screws (group 2), and the 4-rod technique (group 3). Rotational angles of the L1-S1 and L5-S1 segments were measured to study the stability of the 3 lumbosacral fixation constructs compared with the intact spine. Nondestructive, multidirectional flexibility tests that included 4 loading methods followed by a destructive flexural load to failure were performed using an material testing machine. The lumbosacral peak range of motion (ROM) (millimeters or degrees) and ultimate failure load (Nm) of the 3 reconstruction techniques were statistically compared using a 1-way analysis of variance combined with a Student-Newman-Keuls post hoc test. Results.: The average bone mineral density of the 7 specimens was 0.81 ± 0.09 g/cm. The ROM of the 3 fixation constructs was significantly smaller than that of the intact group in all 6 directions (P < 0.05). In lateral bending, the ROM of groups 2 and 3 was significantly smaller than that of group 1 (P < 0.05), but groups 2 and 3 were not significantly different from each other (P > 0.05). In flexion-extension, the ROM of groups 1 and 3 was significantly smaller than group 2 (P < 0.05), but groups 1 and 3 were not significantly different from each other (P > 0.05). In axial rotation, the ROM of group 3 was significantly smaller than those of groups 1 and 2 (P < 0.05), but groups 1 and 2 were not significantly different from each other (P > 0.05). Conclusion.: The 4-rod technique achieved stable biomechanical effects in lumbosacral fixation. At the L5-S1 junction, the 4-rod technique demonstrated better stability than the constructs using S1 screws or S1 screws plus iliac screws. Copyright © 2013 Lippincott Williams & Wilkins.

Chen L.,North Sichuan Medical College | Jiang K.,North Sichuan Medical College | Jiang H.,Second Peoples Hospital of Chengdu | Wei P.,North Sichuan Medical College
Experimental and Therapeutic Medicine | Year: 2014

Frequent acquisition of drug resistance is often associated with the chemotherapy of malignant tumors, including osteosarcoma. A number of studies have demonstrated a critical role for autophagy in osteosarcoma development, therapy and drug resistance. However, the molecular mechanisms underlying the autophagy-mediated chemotherapy resistance of osteosarcoma cells remain largely unknown. In the present study, we determined the autophagy and microRNA-155 (miR-155) expression induced by chemotherapeutic drugs in osteosarcoma cells. Then we determined the promotory role of miR-155 to the chemotherapy-induced autophagy. Our results demonstrated that microRNA-155 (miR-155) expression was highly induced during chemotherapy of osteosarcoma cells, and this was accompanied by upregulated autophagy. The increased miR-155 expression levels upregulated anticancer drug-induced autophagy in osteosarcoma cells and ameliorated the anticancer drug-induced cell proliferation and viability decrease. Therefore, the results of the present study demonstrated that miR-155 mediated drug-resistance in osteosarcoma cells by inducing autophagy. The present study recognized a novel mechanism of chemoresistance in osteosarcoma cancers.

Chen H.,University of Sichuan | Liang B.,University of Sichuan | Tang Y.,University of Sichuan | Xu Z.,Second Peoples Hospital of Chengdu | And 4 more authors.
Chinese Medical Journal | Year: 2012

Background The relationship between the 6-minute walk test (6MWT) and pulmonary function test in stable chronic obstructive pulmonary disease (COPD) remains unclear. We evaluate the correlation of 6MWT and spirometric parameters in stable COPD with different severities. 6MWT data assessed included three variables: the 6-minute walk distance (6MWD), 6-minute walk work (6MWORK), and pulse oxygen desaturation rate (SPO2%). Methods 6MWT and pulmonary function test were assessed for 150 stable COPD patients with different severities. Means and standard deviations were calculated for the variables of interest. Analysis of variance was performed to compare means. Correlation coefficients were calculated for 6MWT data with the spirometric parameters and dyspnea Borg scale. Multiple stepwise regression analysis was used to screen pulmonary function-related predictors of 6MWT data. Results The three variables of 6MWT all varied as the severities of the disease. The 6MWD and 6MWORK both correlated with some spirometric parameters (positive or negative correlation; the absolute value of r ranging from 0.34 to 0.67; P <0.05) in severe and very severe patients, and the SPO2% correlated with the dyspnea Borg scale in four severities (r= -0.33, -0.34, -0.39, -0.53 respectively; P <0.05). The 6MWD was correlated with the 6MWORK in four severities (r=0.56, 0.57, 0.72, 0.81 respectively, P <0.05), and neither of them correlated with the SPO2%. The percent of predicted forced expiratory volume in 1 second (FEV1% predicted) and residual volume to total lung capacity ratio (RV/TLC) were predictors of the 6MWD, and the maximum voluntary ventilation (MVV) was the predictor of the 6MWORK. Conclusions 6MWT correlated with the spirometric parameters in severe and very severe COPD patients. 6MWT may be used to monitor changes of pulmonary function in these patients.

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