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Yuan J.-M.,Second Military Medical University of Chinese | Dang R.-S.,Second Military Medical University of Chinese | Shen M.-R.,Second Military Medical University of Chinese | Zhang C.-S.,Second Military Medical University of Chinese
Journal of Clinical Rehabilitative Tissue Engineering Research

OBJECTIVE: To review the current research of tissue engineered venous valve at home and abroad, to analyze the developing trend of tissue engineered venous valve in the clinical application. METHODS: A computer retrieve was performed among PubMed, ProQuest Health & Medical Complete database, Springer English Academic Journal Full-text database, Elsevier Full-text database between January 2000 and August 2009, with the key words of "tissue engineering venous valve", and the language was limited to English. At the same time, Chongqing VIP database, Qinghua Academic Journals Database, Chinese Biomedical Literature database were also screened on computer by using the key words "tissue engineered venous valve", and the language was limited to Chinese. In addition, the relevant monographs were manually checked. RESULTS: The comprehensive analysis shows that an ideal method to construct tissue engineered venous valve is to combine progenitor cells with multipotent adult progenitor cells for the batched incubation in allogenic acellular vein scaffolds, in vivo environment can be maximally simulated through constant pressure perfusions on the three-dimensional culture system, thus seed cells can well grow into functional tissue engineered venous valve on the scaffold materials. This study provides experimental basis for clinical application of venous valve tissue engineering. CONCLUSION: At present, tissue engineered venous valve research has made considerable progress and encouraging results, brings first lights for deep venous insufficiency for patients, and exhibits wide application in the field of deep venous insufficiency treatment. Source

Wu S.-X.,Second Military Medical University of Chinese | Yu Z.-H.,Second Military Medical University of Chinese | Liu F.,Second Military Medical University of Chinese | Lin H.-Y.,Second Military Medical University of Chinese | And 2 more authors.
Chinese Journal of Tissue Engineering Research

BACKGROUND: Retinoic acid signaling pathways is very important in the formation pf nervous system, specialization of neurons and outgrowth of axons. The recent studies show that, retinoic acid plays an important role in the process of axonal regeneration, but few research reports its exact molecular mechanism. OBJECTIVE: To analyze and summarize the mechanism underlying retinoic acid signaling pathways in the process of axonal regeneration. METHODS: A computer-based online research was conducted among the VIP, CNKI, PubMed, BioMed Centeral, Springer, The Free Medical Journals, EBSCO and Foreign Journals Integration System between January 2000 and December 2013, with the key words of "retinoic acid, the central nervous system, nerve damage, axon regeneration, and mechanism" in Chinese and English. A total of 43 studies addressing the molecular mechanism of retinoic acid in axonal regeneration were screened. According to the supplementary documents, another five references were added. Repetitive research and atypical reports were excluded. RESULTS AND CONCLUSION: Following acute central nervous system injury, axonal regeneration and functional recovery are extremely limited. For proper functionality following injury, axons must regrow, reinnervate their targets, and remyelinate their axons. When the central nervous system injuries occur, retinoic acid signaling pathways express transcription factor retinoic acid receptor β2 to induce axonal regeneration following injury; in dorsal root ganglion neurons, cAMP levels are greatly increased by lentiviral retinoic acid receptor β2 expression and contribute to neurite outgrowth. More recently, retinoic acid-retinoic acid receptor β2 pathways directly transcriptionally repress a member of the inhibitory Nogo receptor complex, Lingo-1, under an axonal growth inhibitory environment in vitro as well as following spinal cord injury in vivo. Through these molecular mechanisms, retinoic acid signaling pathways play its important role in the process of axonal regeneration. Source

Gao G.-Z.,Second Military Medical University of Chinese | Li D.-W.,Second Military Medical University of Chinese | Li X.,Second Military Medical University of Chinese | Sun T.,Second Military Medical University of Chinese
Journal of Clinical Rehabilitative Tissue Engineering Research

BACKGROUND: The small intestine is extremely sensitive to the hypoxia and ischemia, and it would be got severe damage after ischemia/reperfusion injury. Bone marrow mesenchymal stem cells (BMSCs) are multipotent cells which might participate in the repair of damaged tissue through a variety of ways. OBJECTIVE: To investigate colonization and therapeutic effects of allogeneic rat BMSCs transplantation in the intestine of ischemia/reperfusion injury model. METHODS: Wistar female rats were assigned to three groups. The abdominal cavity was opened and then sutured in the sham operation group. Remaining rats were used to establish ischemia/reperfusion models. Rats in the control group were only infused with saline following intestine with ischemia/reperfusion injury. Rats in the treatment group were treated with BMSCs from Wistar male rats via caudal vein. Following treatment, jejunal tissue was harvested at 12, 24 hours, 3, 7, 14 and 28 days separately to make frozen sections. The distribution of donor cells in the receptor intestine was observed under a fluorescence microscope. Reverse transcription-polymerase chain reaction was employed to determine the sex-determining gene (SRY) in male rats. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the jejunal tissues were detected. RESULTS AND CONCLUSION: No donor cell transfused in intestinal villi was detected under the fluorescence microscope. The expression ratio of the SRY gene was 50%, 66.6%, 33.3%, 16.6% at 3, 7, 14, and 28 days respectively. The levels of MDA were lower, but SOD levels were higher in jejunum tissues in the treatment group than the control group on 12, 24 hours, 3 and 7 days. Results have found that BMSCs from allogeneic rats could colonize in the injured intestine of the receptor rat with ischemia/reperfusion injury. The BMSCs transplantation can promote the recovery of intestinal injury. The beneficial effects of BMSCs were primarily mediated via paracrine actions but not by their direct differentiation into target cells. Source

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