Second Hospital of Longyan City

Longyan, China

Second Hospital of Longyan City

Longyan, China
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Xiao J.-N.,Second Hospital of Longyan City | Xiao J.-N.,Humanity | Yan T.-H.,Humanity | Yu R.-M.,Humanity | And 10 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2017

Purpose: Long non-coding RNA (LncRNA) urothelial carcinoma-associated 1 (UCA1) is reported to be dysregulated in hepatocellular carcinoma (HCC) progression. However, the functions of UCA1 in HCC still need further study. The aim is to detect the role of UCA1 involving in HCC cells proliferation and invasion, and epithelial–mesenchymal transition (EMT). Methods: The quantitative real-time PCR was used to detect the UCA1 and miR-203 expression levels in 60 cases’ HCC tissues and adjacent normal tissues. Western blotting analysis was performed to detect the EMT markers E-cadherin, Vimentin and transcription factor Snail1, Snail2 expression. Luciferase reporter assay, RNA immunoprecipitation (RIP) and pull-down assays were used to evaluate whether miR-203 was a target of UCA1. Results: Our results showed that UCA1 was markedly upregulated in HCC tissues and higher UCA1 expression in HCC was positively associated with tumor size, vascular invasion and American Joint Committee on Cancer (AJCC) stage (P < 0.05). Furthermore, gain-of-function and loss-of-function analysis showed that UCA1 knockdown inhibited HCC cells proliferation and invasion in vitro and xenograft tumour growth in vivo. Moreover, UCA1 overexpression promoted cell epithelial–mesenchymal transition (EMT) in HCC via effectively sponging to miR-203 and thereby activating the expression of transcription factor Snail2. Conclusions: Our results identified that UCA1/miR-203/Snail2 pathway might involve in HCC progression. Inhibition of UCA1 acted as a promising therapeutic target for HCC patients. © 2017 Springer-Verlag Berlin Heidelberg


Zhou Z.,Key Laboratory of Tumor Translational Medicine in Fujian Province | Zhou Z.,Fujian Medical University | Jiang J.,Second Hospital of Longyan City | Li J.,Key Laboratory of Tumor Translational Medicine in Fujian Province | And 5 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the enhanced anti-tumor effect of IL-12 through inducing NK cell activition in hepatic carcinoma microenviroment. Methods: The hepatic cacinoma HepG2 cells were subcutaneously injected into NOD/SCID mice,and human peripheral blood lymphocytes(PBL) were introperitoneally injected after tumor formation to establish HCC-huPBL tumor-bearing mouse model. The tumor-bearing mice were randomized into IL-12 group and PBS control group. Mice were intratumoral injected with IL-12, and the changes of tumor volume and body weight as well as general conditions of tumor-bearing mice were observed. ELISA assay was perfomied to examine the expression levels of IL-12 and INFγ in the microenvironment of hepatic carcinoma tissues in tumor-bearing mice, and the aspartate aminotransferase(AST)and alanine minotransferase(ALT)levels in peripheral blood of mice 30 days after IL-12 intra- tumoral injection. Immunohistochemistry assay was used to analyze the expressions of NK-activating receptors: NKG2D, NKp44, NKp30, NKp46,and inhibitory NK receptors: KIR2DL3/CD158b and NKG2A/CD159a in hepatic carcinoma microenvironment after IL-12 treatment. Results: On day 12, 18,24 and 30,the tumor volumes were smaller in the IL-12 group than those in the PBS group ([594.47±205.51]vs[832.10±187.49]mm3,[963.61±427.95]vs[1350.87±468.23]mm3,[1285.02±368.56]vs[1975.49±655.54]mm3,[1903.64±471.34]vs[2568.77±784.68]mm3,P<0.05).The expression levels of IL-12 and IFNγ in the IL-12 group were significantly higher than those in the PBS group ([2.96±1.02]vs[1.35±0.75]pg/ml,[12.26±4.11] vs [7.81±3.46]pg/ml, P<0.05).The serum ALT level significantly increased in the IL-12 group compared to the PBS group on day 7([73.85 ±10.71]vs [41.73±13.13]U/L,P<0.05),and reached a peak at day 14. The expressions of NK-activa-ting receptors NKG2D, NKp44 and NKp30 were statistically higher in the IL-12 group than those in the PBS group (P<0.05),the expression level of NKp46 showed no significant up-regulation, while the expression levels of NK inhibitory receptors CD158b and CD159a were decreased compared to the PBS group (P<0.05). Conclusion: IL-12 intratumoral injection can up-regulate the expressions of NK-activating receptors,IL-12 and IFNγ,and down-regulate the NK inhibitory receptors in the hepatic carcinoma mouse model,therefore effectively inhibiting the tumor growth in mouse model.


Chen G.-Q.,Second Hospital of Longyan City | Luo J.-B.,Second Hospital of Longyan City | Wang G.-Z.,Second Hospital of Longyan City | Ding J.-E.,Second Hospital of Longyan City
Tumor Biology | Year: 2014

Vascular endothelial growth factor (VEGF) plays a crucial role in the regulation of angiogenesis and is involved in the development and metastasis of common cancers. There were several case-controls studies published to assess the associations of VEGF polymorphisms with risk of prostate cancer, but the findings were inconsistent. We performed a meta-analysis to provide a comprehensive assessment of the associations of three VEGF polymorphisms with risk of prostate cancer. The pooled odds ratio (OR) with 95 % confidence interval (95 % CI) was calculated to assess the associations. Eleven individual case-control studies with a total of 5,209 cases of prostate cancer and 5,233 controls were finally included into our meta-analysis. Overall, VEGF rs833061 polymorphism was not associated with risk of prostate cancer (T versus C, OR = 1.14, 95 % CI 0.91-1.44, P = 0.26; TT versus CC, OR = 1.09, 95 % CI 0.67-1.76, P = 0.74; TT versus CC/CT: OR = 1.46, 95 % CI 0.67-3.18, P = 0.34; TT/CT versus CC, OR = 1.08, 95 % CI 0.82-1.43, P = 0.59). VEGF rs3025039 polymorphism was also not associated with risk of prostate cancer (T versus C, OR = 1.03, 95 % CI 0.91-1.16, P = 0.66; TT versus CC, OR = 1.82 95 % CI 0.16-20.53, P = 0.63; TT versus CC/CT, OR = 2.00, 95 % CI 0.18-22.41, P = 0.57; TT/CT versus CC, OR = 0.72, 95 % CI 0.38-1.36, P = 0.31). VEGF rs2010963 polymorphism was not associated with risk of prostate cancer under three models (C versus G, OR = 1.17, 95 % CI 0.92-1.48, P = 0.20; CC versus GG, OR = 2.28, 95 % CI 0.90-5.75, P = 0.08; CC versus GG/GC, OR = 1.57, 95 % CI 0.67-3.68, P = 0.30). In conclusison, current data suggest that those three VEGF polymorphisms are not obviously associated with risk of prostate cancer. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Yan T.-H.,Second Hospital of Longyan City | Lu S.-W.,Second Hospital of Longyan City | Huang Y.-Q.,Second Hospital of Longyan City | Que G.-B.,Second Hospital of Longyan City | And 6 more authors.
Tumor Biology | Year: 2014

Stage Ta/T1 urothelial carcinoma of the bladder (Ta/T1 BC) has a marked tendency to recurrence. Long noncoding RNA HOTAIR has been reported to be expressed in some human cancers such as breast cancer, and it may be positively correlated with patient’s prognosis. The aim of our study was to evaluate the prognostic value of HOTAIR in Ta/ T1 BC. HOTAIR expression in Ta/T1 BC tissues and adjacent normal tissues was collected from 110 patients and measured by real-time quantitative PCR. The relationships between HOTAIR and the clinical pathological characteristics of Ta/ T1 BC patients were analyzed. Immunohistochemistry was done to detect the protein of Wnt inhibitory factor 1 (WIF-1) as well. Ninety out of 110 specimens were detected in HOTAIR high expression. Histological grade and expression levels of HOTAIR were positively correlated with the recurrence rate. HOTAIR expression (hazard ratio 4.712; 95 % CI 2.894–8.714; P<0.001) was an independent predictor of recurrence rate in multivariate Cox regression analysis. HOTAIR expression is correlated with patients’ poor prognosis. A significant inverse correlation between HOTAIR and WIF-1 expression was demonstrated in Ta/T1 BC tissues. The expression levels of HOTAIR are an independent prognostic factor of recurrence in Ta/T1 BC patients. © International Society of Oncology and BioMarkers (ISOBM) 2014.


Yan T.-H.,Second Hospital of Longyan City | Lin Z.-H.,Fujian Medical University | Jiang J.-H.,Fujian Medical University | Jiang J.-H.,Humanity | And 7 more authors.
Archives of Medical Research | Year: 2015

Background and Aims. Matrix metalloproteinase 14 (MMP14) has been identified to play a significant role in several types of cancers, but little is known about the significance of MMP14 in nasopharyngeal carcinoma (NPC) patients. The aim of this study was to explore the association of MMP14 expression with clinicopathologic features and prognosis in NPC. Methods. MMP14 mRNA and protein expressions were examined in NPC and nasopharyngeal tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of MMP14 expression levels with clinical features and prognosis of NPC patients was analyzed. Results. MMP14 mRNA expression was markedly higher in NPC tissues than in nasopharyngeal epithelium tissues (p = 0.002). Using immunohistochemistry, staining for MMP14 protein was found in the normal nasopharyngeal epithelial cells and malignant epithelial cells, but increased expression of MMP14 was observed in NPC samples compared with normal nasopharyngeal epithelium samples (p = 0.027). In addition, high levels of MMP14 protein were positively correlated with the status of clinical stage (p = 0.009), N classification (p = 0.006), and distant metastasis (p = 0.005) of NPC patients. Patients with higher MMP14 expression had a significantly shorter overall survival time than did patients with low MMP14 expression. Multivariate analysis indicated that the level of MMP14 expression was an independent prognostic indicator (p < 0.001) for the survival of patients with NPC. Conclusions. MMP14 overexpression is a potentially unfavorable prognostic factor for NPC patients. © 2015 IMS.


Yan T.,Second Hospital of Longyan City | Lin Z.,Fujian Medical University | Jiang J.,Fujian Medical University | Jiang J.,Humanity | And 8 more authors.
American Journal of Translational Research | Year: 2015

Matrix metalloproteinase 14 (MMP14) has been shown to play a significant role in several types of cancers, but little is known about the function of MMP14 in nasopharyngeal carcinoma (NPC) carcinogenesis. The aim of this study was to investigate the role of MMP14 in NPC using NPC tumor samples or tissue microarray. We have shown that MMP14 was increased in NPC samples compared with normal nasopharynx (NP) tissues in microar-ray data (GSE13597). Both MMP14 mRNA and protein expression were markedly higher in NPC tissues than in NP tissues. High levels of MMP14 protein were found positively correlate with the status of late clinical stages of tumor and tumor with lymph node metastasis. Moreover, we have shown that MMP14 expression promoted the cell migration and invasion of NPC cells in vitro and regulated the expression of EMT-associated genes. Our data demonstrated that MMP14 plays an important role in regulation of migration and invasion of NPC cells, and constitutes a potential novel therapeutic target for NPC. © 2015, E-Century Publishing Corporation. All rights reserved.


Chen Z.-K.,Fujian Medical University | Cai M.-X.,Second Hospital of Longyan City | Yang J.,Fujian Medical University | Lin L.-W.,Fujian Medical University | And 5 more authors.
Medical Oncology | Year: 2012

To investigate the antiangiogenic effect of sustained-release poly (lactic-co-glycolic acid) microspheres containing docetaxel (PMCD) in human hepatoma xenograft. PMCD were prepared by solvent evaporation method with an encapsulation efficiency of 98.7% and a release period of about 3 weeks in vitro. PMCD were intratumorally injected once for mice bearing a human hepatocellular carcinoma. On day 21 post-treatment, the inhibition rate of tumor growth was 72.7% in the high-dose group, indicating a significant antitumor activity. Meanwhile, excellent antiangiogenic effect was observed based on the contrast-enhanced ultrasonography as well as microvessel density determination. Additionally, the realtime fluorescence quantitative PCR revealed that the expressions of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiopoietin-2 (Ang2) genes were down-regulated significantly. Interstitial chemotherapy using PMCD was highly effective and safe for the treatment of the human hepatoma xenograft and that decreasing angiogenesis could be one of the most important mechanisms involved in the antitumor activity. © 2011 Springer Science+Business Media, LLC.


PubMed | Second Hospital of Longyan City
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

Vascular endothelial growth factor (VEGF) plays a crucial role in the regulation of angiogenesis and is involved in the development and metastasis of common cancers. There were several case-controls studies published to assess the associations of VEGF polymorphisms with risk of prostate cancer, but the findings were inconsistent. We performed a meta-analysis to provide a comprehensive assessment of the associations of three VEGF polymorphisms with risk of prostate cancer. The pooled odds ratio (OR) with 95% confidence interval (95% CI) was calculated to assess the associations. Eleven individual case-control studies with a total of 5,209 cases of prostate cancer and 5,233 controls were finally included into our meta-analysis. Overall, VEGF rs833061 polymorphism was not associated with risk of prostate cancer (T versus C, OR=1.14, 95% CI 0.91-1.44, P=0.26; TT versus CC, OR=1.09, 95% CI 0.67-1.76, P=0.74; TT versusOR=1.46, 95% CI 0.67-3.18, P=0.34; TT/CT versus CC, OR=1.08, 95% CI 0.82-1.43, P=0.59). VEGF rs3025039 polymorphism was also not associated with risk of prostate cancer (T versus C, OR=1.03, 95% CI 0.91-1.16, P=0.66; TT versus CC, OR=1.82 95% CI 0.16-20.53, P=0.63; TT versus CC/CT, OR=2.00, 95% CI 0.18-22.41, P=0.57; TT/CT versus CC, OR=0.72, 95% CI 0.38-1.36, P=0.31). VEGF rs2010963 polymorphism was not associated with risk of prostate cancer under three models (C versus G, OR=1.17, 95% CI 0.92-1.48, P=0.20; CC versus GG, OR=2.28, 95% CI 0.90-5.75, P=0.08; CC versus GG/GC, OR=1.57, 95% CI 0.67-3.68, P=0.30). In conclusison, current data suggest that those three VEGF polymorphisms are not obviously associated with risk of prostate cancer.


PubMed | Second Hospital of Longyan City
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

Stage Ta/T1 urothelial carcinoma of the bladder (Ta/T1 BC) has a marked tendency to recurrence. Long noncoding RNA HOTAIR has been reported to be expressed in some human cancers such as breast cancer, and it may be positively correlated with patients prognosis. The aim of our study was to evaluate the prognostic value of HOTAIR in Ta/T1 BC. HOTAIR expression in Ta/T1 BC tissues and adjacent normal tissues was collected from 110 patients and measured by real-time quantitative PCR. The relationships between HOTAIR and the clinical pathological characteristics of Ta/T1 BC patients were analyzed. Immunohistochemistry was done to detect the protein of Wnt inhibitory factor 1 (WIF-1) as well. Ninety out of 110 specimens were detected in HOTAIR high expression. Histological grade and expression levels of HOTAIR were positively correlated with the recurrence rate. HOTAIR expression (hazard ratio 4.712; 95 % CI 2.894-8.714; P<0.001) was an independent predictor of recurrence rate in multivariate Cox regression analysis. HOTAIR expression is correlated with patients poor prognosis. A significant inverse correlation between HOTAIR and WIF-1 expression was demonstrated in Ta/T1 BC tissues. The expression levels of HOTAIR are an independent prognostic factor of recurrence in Ta/T1 BC patients.


PubMed | Fujian Medical University and Second Hospital of Longyan City
Type: Journal Article | Journal: Archives of medical research | Year: 2015

Matrix metalloproteinase 14 (MMP14) has been identified to play a significant role in several types of cancers, but little is known about the significance of MMP14 in nasopharyngeal carcinoma (NPC) patients. The aim of this study was to explore the association of MMP14 expression with clinicopathologic features and prognosis in NPC.MMP14 mRNA and protein expressions were examined in NPC and nasopharyngeal tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of MMP14 expression levels with clinical features and prognosis of NPC patients was analyzed.MMP14 mRNA expression was markedly higher in NPC tissues than in nasopharyngeal epithelium tissues (p = 0.002). Using immunohistochemistry, staining for MMP14 protein was found in the normal nasopharyngeal epithelial cells and malignant epithelial cells, but increased expression of MMP14 was observed in NPC samples compared with normal nasopharyngeal epithelium samples (p = 0.027). In addition, high levels of MMP14 protein were positively correlated with the status of clinical stage (p = 0.009), N classification (p = 0.006), and distant metastasis (p = 0.005) of NPC patients. Patients with higher MMP14 expression had a significantly shorter overall survival time than did patients with low MMP14 expression. Multivariate analysis indicated that the level of MMP14 expression was an independent prognostic indicator (p < 0.001) for the survival of patients with NPC.MMP14 overexpression is a potentially unfavorable prognostic factor for NPC patients.

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