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Tang L.,Shanghai University | Gao X.,Shanghai University | Xu C.-L.,Shanghai University | Chen G.-Q.,Second Hospital of Longyan City | And 3 more authors.
Academic Journal of Second Military Medical University | Year: 2016

Objective To indentify the high risk factors of bone metastasis in Chinese patients at the initial stage of prostate cancer (PCa) diagnosis, so as to elucidate the characteristics of patients with very low risk of bone metastasis at the initial stage of prostate cancer. Methods A consecutive series of 496 patients with newly diagnosed PCa between 2010 and 2015 were enrolled in the present study. All the patients were subjected to ECT scan for presence of bone metastasis (BM) using total-body 99mTc MDP scintigraphy regardless of baseline PCa characteristics. Factors including the age of diagnosis, prostate specific antigen (PSA) level at diagnosis, Gleason score, clinical T stage, bone scan and CT/MRI results were analyzed. Univariate and multivariate logistic regression analyses were performed to identify the predictors of bone metastases. Results Of the 496 patients, 81 patients (16.3%) had bone metastases. The PSA levels of patients with BM were significantly higher than those without BM (P<0.001). The mean age of the patients with BM was not significantly older than that of the patients without BM. Patients with higher PSA level, clinical T stage or Gleason score showed a significantly higher risk of BM (P<0.001). Univariate logistic regression analysis showed that PSA>20 ng/mL at diagnosis, clinical stage at T3-T4 and Gleason score≥8 were the risk factors of bone metastasis in PCa patients. The multivariate analysis showed that the PSA level>50 ng/mL and the Gleason score≥8 were the independent predictors of bone metastases. No bone metastasis was found in 79 patients with PSA≤20 ng/mL and at the same time with Gleason score≤6. Conclusion The bone metastases rate is very low in Chinese patients with a PSA level ≤20 ng/mL and at the same time with Gleason score≤6, so a bone scan is not necessary as a routine for these patients with newly diagnosed prostate cancer. © 2015, Second Military Medical University Press. All rights reserved. Source


Chen Z.-K.,Fujian Medical University | Cai M.-X.,Second Hospital of Longyan City | Yang J.,Fujian Medical University | Lin L.-W.,Fujian Medical University | And 5 more authors.
Medical Oncology | Year: 2012

To investigate the antiangiogenic effect of sustained-release poly (lactic-co-glycolic acid) microspheres containing docetaxel (PMCD) in human hepatoma xenograft. PMCD were prepared by solvent evaporation method with an encapsulation efficiency of 98.7% and a release period of about 3 weeks in vitro. PMCD were intratumorally injected once for mice bearing a human hepatocellular carcinoma. On day 21 post-treatment, the inhibition rate of tumor growth was 72.7% in the high-dose group, indicating a significant antitumor activity. Meanwhile, excellent antiangiogenic effect was observed based on the contrast-enhanced ultrasonography as well as microvessel density determination. Additionally, the realtime fluorescence quantitative PCR revealed that the expressions of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiopoietin-2 (Ang2) genes were down-regulated significantly. Interstitial chemotherapy using PMCD was highly effective and safe for the treatment of the human hepatoma xenograft and that decreasing angiogenesis could be one of the most important mechanisms involved in the antitumor activity. © 2011 Springer Science+Business Media, LLC. Source


Yan T.-H.,Second Hospital of Longyan City | Lu S.-W.,Second Hospital of Longyan City | Huang Y.-Q.,Second Hospital of Longyan City | Que G.-B.,Second Hospital of Longyan City | And 6 more authors.
Tumor Biology | Year: 2014

Stage Ta/T1 urothelial carcinoma of the bladder (Ta/T1 BC) has a marked tendency to recurrence. Long noncoding RNA HOTAIR has been reported to be expressed in some human cancers such as breast cancer, and it may be positively correlated with patient’s prognosis. The aim of our study was to evaluate the prognostic value of HOTAIR in Ta/ T1 BC. HOTAIR expression in Ta/T1 BC tissues and adjacent normal tissues was collected from 110 patients and measured by real-time quantitative PCR. The relationships between HOTAIR and the clinical pathological characteristics of Ta/ T1 BC patients were analyzed. Immunohistochemistry was done to detect the protein of Wnt inhibitory factor 1 (WIF-1) as well. Ninety out of 110 specimens were detected in HOTAIR high expression. Histological grade and expression levels of HOTAIR were positively correlated with the recurrence rate. HOTAIR expression (hazard ratio 4.712; 95 % CI 2.894–8.714; P<0.001) was an independent predictor of recurrence rate in multivariate Cox regression analysis. HOTAIR expression is correlated with patients’ poor prognosis. A significant inverse correlation between HOTAIR and WIF-1 expression was demonstrated in Ta/T1 BC tissues. The expression levels of HOTAIR are an independent prognostic factor of recurrence in Ta/T1 BC patients. © International Society of Oncology and BioMarkers (ISOBM) 2014. Source


Zhou Z.,Key Laboratory of Tumor Translational Medicine in Fujian Province | Zhou Z.,Fujian Medical University | Jiang J.,Second Hospital of Longyan City | Li J.,Key Laboratory of Tumor Translational Medicine in Fujian Province | And 5 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the enhanced anti-tumor effect of IL-12 through inducing NK cell activition in hepatic carcinoma microenviroment. Methods: The hepatic cacinoma HepG2 cells were subcutaneously injected into NOD/SCID mice,and human peripheral blood lymphocytes(PBL) were introperitoneally injected after tumor formation to establish HCC-huPBL tumor-bearing mouse model. The tumor-bearing mice were randomized into IL-12 group and PBS control group. Mice were intratumoral injected with IL-12, and the changes of tumor volume and body weight as well as general conditions of tumor-bearing mice were observed. ELISA assay was perfomied to examine the expression levels of IL-12 and INFγ in the microenvironment of hepatic carcinoma tissues in tumor-bearing mice, and the aspartate aminotransferase(AST)and alanine minotransferase(ALT)levels in peripheral blood of mice 30 days after IL-12 intra- tumoral injection. Immunohistochemistry assay was used to analyze the expressions of NK-activating receptors: NKG2D, NKp44, NKp30, NKp46,and inhibitory NK receptors: KIR2DL3/CD158b and NKG2A/CD159a in hepatic carcinoma microenvironment after IL-12 treatment. Results: On day 12, 18,24 and 30,the tumor volumes were smaller in the IL-12 group than those in the PBS group ([594.47±205.51]vs[832.10±187.49]mm3,[963.61±427.95]vs[1350.87±468.23]mm3,[1285.02±368.56]vs[1975.49±655.54]mm3,[1903.64±471.34]vs[2568.77±784.68]mm3,P<0.05).The expression levels of IL-12 and IFNγ in the IL-12 group were significantly higher than those in the PBS group ([2.96±1.02]vs[1.35±0.75]pg/ml,[12.26±4.11] vs [7.81±3.46]pg/ml, P<0.05).The serum ALT level significantly increased in the IL-12 group compared to the PBS group on day 7([73.85 ±10.71]vs [41.73±13.13]U/L,P<0.05),and reached a peak at day 14. The expressions of NK-activa-ting receptors NKG2D, NKp44 and NKp30 were statistically higher in the IL-12 group than those in the PBS group (P<0.05),the expression level of NKp46 showed no significant up-regulation, while the expression levels of NK inhibitory receptors CD158b and CD159a were decreased compared to the PBS group (P<0.05). Conclusion: IL-12 intratumoral injection can up-regulate the expressions of NK-activating receptors,IL-12 and IFNγ,and down-regulate the NK inhibitory receptors in the hepatic carcinoma mouse model,therefore effectively inhibiting the tumor growth in mouse model. Source


Yan T.-H.,Second Hospital of Longyan City | Lin Z.-H.,Fujian Medical University | Jiang J.-H.,Fujian Medical University | Jiang J.-H.,Humanity | And 7 more authors.
Archives of Medical Research | Year: 2015

Background and Aims. Matrix metalloproteinase 14 (MMP14) has been identified to play a significant role in several types of cancers, but little is known about the significance of MMP14 in nasopharyngeal carcinoma (NPC) patients. The aim of this study was to explore the association of MMP14 expression with clinicopathologic features and prognosis in NPC. Methods. MMP14 mRNA and protein expressions were examined in NPC and nasopharyngeal tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of MMP14 expression levels with clinical features and prognosis of NPC patients was analyzed. Results. MMP14 mRNA expression was markedly higher in NPC tissues than in nasopharyngeal epithelium tissues (p = 0.002). Using immunohistochemistry, staining for MMP14 protein was found in the normal nasopharyngeal epithelial cells and malignant epithelial cells, but increased expression of MMP14 was observed in NPC samples compared with normal nasopharyngeal epithelium samples (p = 0.027). In addition, high levels of MMP14 protein were positively correlated with the status of clinical stage (p = 0.009), N classification (p = 0.006), and distant metastasis (p = 0.005) of NPC patients. Patients with higher MMP14 expression had a significantly shorter overall survival time than did patients with low MMP14 expression. Multivariate analysis indicated that the level of MMP14 expression was an independent prognostic indicator (p < 0.001) for the survival of patients with NPC. Conclusions. MMP14 overexpression is a potentially unfavorable prognostic factor for NPC patients. © 2015 IMS. Source

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