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Li J.,Zhejiang University | Wu T.,Zhejiang University | Xu Z.,Zhejiang University | Gu X.,Second Hospital of Jiaxing
European Journal of Orthopaedic Surgery and Traumatology | Year: 2014

The aim of this study was to explore the application value of the lower limbs robot-assisted training system for post-total knee replacement (TKR) gait rehabilitation. A total of 60 patients with osteoarthritis of the knee were equally randomized into the traditional and robot-assisted rehabilitation training groups within 1 week after TKR. All patients received 2-week training. Scores of hospital for special surgery (HSS), knee kinesthesia grades, knee proprioception grades, functional ambulation (FAC) scores, Berg balance scores, 10-m sitting-standing time, and 6-min walking distances were compared between the groups. The HSS score, Berg score, 10-m sitting-standing time, and 6-min walking distance of the robot-assisted training group were significantly higher than the control group (P < 0.05). Its knee kinesthesia grade, knee proprioception grade, and FAC score were better than the control group but not significantly (P > 0.05). Lower limbs robot-assisted rehabilitation training improves post-TKR patients' knee proprioception and stability more effectively compared with the traditional method. It improves patients' gait and symptoms, increases their walking speed, and prolongs their walking distances, which benefit their return to family and society. © 2013 Springer-Verlag France.

Zou N.,Zhejiang University | Wu X.,Zhejiang University | Jin Y.-Y.,Zhejiang University | He M.-Z.,Second Hospital of Jiaxing | And 6 more authors.
Journal of Membrane Biology | Year: 2013

Pancreatic beta cells act as glucose sensors, in which intracellular ATP ([ATP]i) are altered with glucose concentration change. The characterization of voltage-gated sodium channels under different [ATP] i remains unclear. Here, we demonstrated that increasing [ATP] i within a certain range of concentrations (2-8 mM) significantly enhanced the voltage-gated sodium channel currents, compared with 2 mM cytosolic ATP. This enhancement was attenuated by even high intracellular ATP (12 mM). Furthermore, elevated ATP modulated the sodium channel kinetics in a dose-dependent manner. Increased [ATP]i shifted both the current-voltage curve and the voltage-dependent inactivation curve of sodium channel to the right. Finally, the sodium channel recovery from inactivation was significantly faster when the intracellular ATP level was increased, especially in 8 mM [ATP]i, which is an attainable concentration by the high glucose stimulation. In summary, our data suggested that elevated cytosolic ATP enhanced the activity of Na+ channels, which may play essential roles in modulating β cell excitability and insulin release when blood glucose concentration increases. © 2013 Springer Science+Business Media New York.

Wu H.,Zhejiang University | Zhu S.,Zhejiang University | Yu S.,Zhejiang University | Ding G.,Zhejiang University | And 8 more authors.
Journal of Urology | Year: 2015

Purpose Uroseptic shock is a life threatening complication after upper urinary tract endoscopic lithotripsy. Prompt diagnosis and treatment are paramount for patient survival. We identified a practical predictor of upper urinary tract endoscopic lithotripsy induced uroseptic shock in an animal model. We validated the results by retrospectively reviewing multicenter clinical records. Materials and Methods We ligated the rabbit ureter and injected Escherichia coli solution in the renal pelvis to create a uroseptic shock model. White blood count and other parameters were measured at different intervals and bacterial concentrations. Results were compared with clinical findings in 48 patients who experienced uroseptic shock after upper urinary tract endoscopic lithotripsy at a total of 8 medical centers. We used a ROC curve to evaluate the predictive value of parameters for uroseptic shock. Results We established a stable rabbit model by injecting 9.0 × 108 cfu/ml E. coli in the renal pelvis at a dose of 2 ml/kg. In patients and rabbits with uroseptic shock the white blood count decreased in the first 2 hours after the procedure. The ROC curve showed that the white blood count threshold within the first 2 hours of uroseptic shock was 2.85 × 109/L. Sensitivity and specificity for predicting uroseptic shock were 95.9% and 92.7%, respectively. Conclusions The white blood count decreased drastically within the first 2 hours during the development of uroseptic shock. This could be an ideal predictor of uroseptic shock after upper urinary tract endoscopic lithotripsy. In patients with high risk factors for uroseptic shock the white blood count should be measured within 2 hours after upper urinary tract endoscopic lithotripsy. © 2015 American Urological Association Education and Research, Inc.

Zhu Y.,Second Hospital of Jiaxing | Xiao X.,First Peoples Hospital of Kunshan | Dong L.,Second Hospital of Jiaxing | Liu Z.,Guangxi Medical University
Tumor Biology | Year: 2012

MicroRNAs are small noncoding RNA molecules that control the expression of target genes. Our previous studies show that let-7a decreased in gastric carcinoma and that up-regulation of let-7a by gene augmentation inhibited gastric carcinoma cell growth both in vitro and in vivo, whereas it remains largely unclear as to how let-7a affects tumor growth. In this study, proteins associated with the function of let-7a were detected in high-throughput screening. The cell line of SGC-7901 stably overexpressing let-7a was successfully established by gene clone. Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. Ten differential protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and they may be the proteins associated with let-7a function. The overexpressed proteins include antioxidant protein 2, insulin-like growth factor binding protein 2, protein disulfide isomerase A2, C-1-tetrahydrofolate synthase, cyclin-dependent kinase inhibitor1 (CDKN1) and Rho-GTPase activating protein 4. The underexpressed proteins consisted of S-phase kinase-associated protein 2 (Spk2), platelet membrane glycoprotein, fibronectin and Cks1 protein. Furthermore, the different expression levels of the partial proteins (CDKN1,Spk2 and Fibronectin) were confirmed by Western blot analysis. The data suggest that these differential proteins are involved in novel let-7a signal pathway, and these findings provided the basis to comprehensively investigate the functional mechanisms of let-7a in gastric carcinoma. © 2012 International Society of Oncology and BioMarkers (ISOBM).

Tian Y.,Zhejiang University | Tian Y.,Zhejiang Chinese Medical University | Wang Y.-F.,Second Hospital of Jiaxing | Du H.-G.,Zhejiang Chinese Medical University | And 3 more authors.
Surgical and Radiologic Anatomy | Year: 2015

Persistent proatlantal artery is one rare kind of persistent primitive anastomoses between carotid and basilar vascular system. This case firstly introduces a type I proatlantal artery with complex vascular anomalies of bilateral vertebral arteries and a ruptured aneurysm, which is extremely uncommon. A 43-year-old female was hospitalised for SAH and ventricular hematocele. The subsequent digital subtraction angiography and computed tomography angiography revealed a type I proatlantal artery which arises from left internal carotid artery, associating with a hypoplastic right vertebral artery, an aplastic left vertebral artery and a ruptured left posterior inferior cerebellar artery aneurysm. An interventional procedure was taken later. The present case raises awareness on the incidence of persistent primitive anastomoses which combined other complex vascular anomalies before surgical or interventional procedures, especially in view of unique blood supply to posterior circulation from the primitive vessel. © 2014, Springer-Verlag France.

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