Li X.,China Japan Union Hospital |
Wang S.,China Japan Union Hospital |
Chen Y.,Second Hospital |
Liu G.,Jilin University |
Yang X.,Jilin University
Onkologie(Czech Republic) | Year: 2013
Aim: To determine the degree of CD40 overexpression in sacral chordomas and its correlation with tumor recurrence. Methods: CD40 or CD31 overexpression was determined by immunohistochemical staining; the microvessel density (MVD) was calculated according to the CD31 expression. The correlation of CD40 over-expression with tumor recurrence was analyzed. Results: 56% of the specimens from 36 cases of sacral chordomas overexpressed CD40, which is a significantly higher percentage than for the 2 specimens in 10 in normal notochordal tissue (p < 0.05). 36.84% of the specimens of the 19 recurrent cases were CD40 overexpressing, in contrast to less than 76.47% in the no-recurrence group (p < 0.05). Multivariate analysis demonstrated that CD40 overexpression and the resection margins were independent factors contributing to tumor recurrence. The MVD value was 25.71 ± 8.86 mm-2 in the sacral chordomas and more than 6.63 ± 2.45 mm-2 in the normal embryonic notochord tissue (p < 0.01). The MVD value in the recurrence group (30.08 ± 7.11 mm-2) was significantly higher than that of the no-recurrence group (20.82 ± 8.18 mm-2; p < 0.05). But the MVD value was significantly lower in the CD40-overexpressing group than in the CD40-less expressing group (p < 0.05). Conclusions: CD40 was overexpressed in sacral chordomas, and the overexpression was not dependent on the intratumoral MVD. CD40 overexpression was correlated with low recurrence of the tumor, implying that CD40 plays an important role in the antitumor response against sacral chordomas and in the inhibition of tumor recurrence. © 2013 S. Karger GmbH, Freiburg.
Li X.W.,Second Hospital |
Li C.L.,Mudanjiang Medical College |
Liang W.D.,Wenzhou Medical College |
Bi Y.T.,Wenzhou Medical College |
And 2 more authors.
Chinese Medical Journal | Year: 2014
Background Protectin D1 (PD1), derived from docosahexaenoic acid, has been shown to control and resolve inflammation in some experimental models of inflammatory disorders. We investigated the protective roles of protectin D1 in pulmonary inflammation and lung injury induced by lipopolysaccharide (LPS). Methods Mice were randomly assigned to six groups (n=6 per group): sham-vehicle group, sham-PD1 group, sham-zVAD-fmk group, LPS-vehicle group, LPS-PD1 group, and LPS-PD1-zVAD-fmk group. Mice were injected intratracheally with 3 mg/kg LPS or saline, followed 24 hours later by intravenous injection of 200 μg/mouse PD1 or vehicle. At the same time, some mice were also injected intraperitoneally with the pan-caspase inhibitor zVAD-fmk. Seventy-two hours after LPS challenge, samples of pulmonary tissue and bronchoalveolar lavage fluid were collected. Optical microscopy was used to examine pathological changes in lungs. Cellularity and protein concentration in bronchoalveolar lavage fluid were analyzed. Lung wet/dry ratios and myeloperoxidase activity were measured. Apoptosis of neutrophils in bronchoalveolar lavage fluid (BALF) was also evaluated by flow cytometry. Results Intratracheal instillation of LPS increased neutrophil counts, protein concentration in bronchoalveolar lavage fluid and myeloperoxidase activity, it induced lung histological injury and edema, and also suppressed apoptosis of neutrophils in BALF. Posttreatment with PD1 inhibited LPS-evoked changes in BALF neutrophil counts and protein concentration and lung myeloperoxidase activity, with the outcome of decreased pulmonary edema and histological injury. In addition, PD1 promoted apoptosis of neutrophils in BALF. The beneficial effects of PD1 were blocked by zVAD-fmk. Conclusion Posttreatment with PD1 enhances resolution of lung inflammation during LPS-induced acute lung injury by enhancing apoptosis in emigrated neutrophils, which is, at least in part, caspase-dependent.
Zhang X.,Anhui Medical University |
Zhang X.,Fudan University |
Tang H.,Anhui Medical University |
Jin X.,Anhui Medical University |
And 67 more authors.
Nature Genetics | Year: 2014
To explore the contribution of functional coding variants to psoriasis, we analyzed nonsynonymous single-nucleotide variants (SNVs) across the genome by exome sequencing in 781 psoriasis cases and 676 controls and through follow-up validation in 1,326 candidate genes by targeted sequencing in 9,946 psoriasis cases and 9,906 controls from the Chinese population. We discovered two independent missense SNVs in IL23R and GJB2 of low frequency and five common missense SNVs in LCE3D, ERAP1, CARD14 and ZNF816A associated with psoriasis at genome-wide significance. Rare missense SNVs in FUT2 and TARBP1 were also observed with suggestive evidence of association. Single-variant and gene-based association analyses of nonsynonymous SNVs did not identify newly associated genes for psoriasis in the regions subjected to targeted resequencing. This suggests that coding variants in the 1,326 targeted genes contribute only a limited fraction of the overall genetic risk for psoriasis. © 2014 Nature America, Inc.
Liu F.-B.,Guangzhou University of Chinese Medicine |
Chen X.-L.,Guangzhou University of Chinese Medicine |
Guo L.,Second Hospital |
Liu X.-B.,Guangzhou University of Chinese Medicine
Chinese Journal of Integrative Medicine | Year: 2012
Objective: To evaluate a scale of patient-reported outcomes for the assessment of myasthenia gravis patients (MG-PRO) in China. Methods: A total of 100 MG patients were interviewed for the field testing. Another 56 MG patients were selected and assessed with the MG-PRO scale before treatment and at 1, 2 and 4 weeks after treatment. The classical test theory and item response theory (IRT) were used to assess the psychometric characteristics of the MG-PRO scale. Results: The MG-PRO scale included 4 dimensions: physical, psychological, social environment, and treatment. Confirmatory factor analysis showed that each dimension was consistent with the theoretical construct. The scores of the physical and psychological dimensions increased significantly at 1 week after treatment (P<0.05). All the dimension scores and the MG-PRO score increased significantly at 2 and 4 weeks after treatment (P<0.05). IRT showed that person separation indices were greater than 0.8, most of the item fit residual statistics were within ±2.5, and no item had uniform or non-uniform differential item functioning (DIF) between gender and age (<40, 40). Conclusions: The MG-PRO scale is valid for measuring the quality of life (QOL) of MG patients, with good reliability, validity, responsiveness, and good psychometric characteristics from IRT. It can be applied to evaluate the QOL of MG patients and to assess treatment effects in clinical trials. © 2011 The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg.
Liu H.-L.,Zhejiang Province Tongde Hospital |
Liu Y.,Zhejiang University |
Liu M.,Second Hospital |
Wang T.,Zhejiang University |
Tuya A.,Chungnam National University
Chinese Physics Letters | Year: 2013
Ni silicide thermal stability is improved by the use of a Ni-V (nickel vanadium) alloy target. The relationship between the formation temperature and the thermal stability of Ni silicide is investigated. The sheet resistance after the formation of Ni silicide with the Ni-V shows stable characteristics up to a rapid-thermal-process temperature of 700°C, while degradation of sheet resistance starts at that temperature in the case of pure-Ni. Moreover, the thermal stability improvement is demonstrated by the post-silicidation annealing. It is considered that the thermal robustness of Ni-V silicide is highly dependent on the formation temperature. With the increasing silicidation temperature (around 700°C), more thermally stable Ni silicide is formed in comparison to the low-temperature case using the Ni-V. A Ni-V alloy target is utilized to form Ni silicide. The V and the V-trap complexes are explained to block the transformation from NiSi to NiSi2 so as to improve the Ni silicide thermal stability. © 2013 Chinese Physical Society and IOP Publishing Ltd.