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Chen F.-H.,PLA Second Artillery General Hospital | Wang X.-R.,PLA Second Artillery General Hospital | Wu L.-F.,Tianjin Medical University | Ou H.-L.,PLA Second Artillery General Hospital | And 5 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To discover radiosensitization of Tat-SmacN7 fusion peptide to human non-small cell lung cancer cell line H460 and its mechanism. METHODS: The WST-1 assay was used to detect toxicity of Tat-SamcN7 to H460 cell line; Clonogenic assay was done to examine the radiosensitization of Tat-SamcN7 to H460; Flow cytometry were used to examine the apoptosis of H460 cell line. Activation of Caspase-3, Caspase-8 and Caspase-9 was detected by Caspase activity assays based on ELISA. RESULTS: Tat-SmacN7 fusion peptide with concentration range of 10 nmol/L to 100 μmol/L was co-cultured with H460 cells, cell survival rates were 80% or more; Tat-SmacN7 fusion peptide (20 μmol/L) and H460 cells were co-cultured, the cell radiosensitizing ratio was 1.63; Tat-SmacN7 (20 μmol/L) had main effect to cell apoptotic rate [(10.87±0.45)%, F=54.389, P<0.001]; Radiation had main effect to cell apoptotic rate [(19.83±0.75)%, F=641.516, P<0.001]; They had interaction (F=65.756, P<0.001). Tat-SmacN7 had main effect on the activity of Caspase-3 (1.418±0.064, F=67.029, P<0.001); Radiation had main effect on activity of Caspase-3 (1.188±0.102, F=29.509, P=0.001); They had interaction (F= 11.328, P=0.010). Tat-SmacN7 had main effect on activity of Caspase-8 (1.867±0.208, F=149. 705, P<0.001); Radiation had main effect on activity of Caspase-8 (1.227±0.123, F=30.418, P=0.001); They had interaction(F=10.663, P=0.011). Tat-SmacN7 had main effect on activity of Caspase-9 (1.672±0.075, F=127.027, P<0.001); Radiation had main effect on activity of Caspase-9(1.279±0.141, F=44.281, P<0.001); They had interaction (F= 9.782, P=0.014). CONCLUSION: Tat-SmacN7 does not have toxicity to H460 cell line used as a single agent, but can increase the sensitivity of H460 cell to radiation, and the radiosensitization have relation with the increase of activity of Caspase-3, Caspase-8 and Caspase-9.


Wang X.,Liaoning Medical University | Shen H.,PLA Second Artillery General Hospital | Yang Y.-J.,PLA Second Artillery General Hospital | Li Z.-R.,PLA Second Artillery General Hospital
Acta Anatomica Sinica | Year: 2015

Objective To analyse the condylar morphology and location of the scissors-bite patients by cone beam computed tomography (CBCT),and investigate the remodeling changes of condylar after scissors-bite side solved. Methods Twenty cases between 18-28 years old with unilateral scissors-bite were selected as the experimental group and 25 cases between 18-30 years old with Class I malocclusion were selected as the control group. All the forty-five subjects had no orthodontic treatment before. Both groups had three-dimensional CBCT imaging with examvision system. Different measurement points and lines of the condylar morphology and location, and 7 measured values were statistically analyzed. Results None statistically significant was observed in control group (P > 0. 05), The mandibular fossa depth and the condylar height showed statistically significant differences between the scissors-bite and non-scissors-bite sides(t = 13. 271, (= 15. 278, P <0. 01). After treatment and kept stable for 3 month, statistical significances of anterior joint space and posterior joint space were found as compared with before (t = 5. 524,t= - 5. 119, P < 0. 01). Conclusion There are differences in condylar morphology and location of unilateral scissors-bite posterior molar patients, after scissors-bite solved for 3 month. There is a tendency to more downward and forward positions in fossa, and there are no special characteristic changes in the condylar morphology observed in this study.


PubMed | PLA Second Artillery General Hospital
Type: Journal Article | Journal: World journal of gastroenterology | Year: 2012

To investigate the incidence of clinically detected port-site metastasis (PSM) in patients who underwent robotic surgery for biliary malignancies.Using a prospective database, the patients undergoing fully robotic surgery for biliary malignancies between January 2009 and January 2011 were included. Records of patients with confirmed malignancy were reviewed for clinicopathological data and information about PSM.Sixty-four patients with biliary tract cancers underwent robotic surgery, and sixty patients met the inclusion criteria. The median age was 67 year (range: 40-85 year). During a median 15-mo follow-up period, two female patients were detected solitary PSM after robotic surgery. The incidence of PSM was 3.3%. Patient 1 underwent robotic anatomatic left hemihepatectomy and extraction of biliary tumor thrombi for an Klatskin tumor. She had a subcutaneous mass located at the right lateral abdominal wall near a trocar scar. Patient 2 underwent robotic pancreaticoduodenectomy for distal biliary cancer. She had two metachronous subcutaneous mass situated at the right lateral abdominal wall under a same trocar scar at 7 and 26 mo. The pathology of the excised PSM masses confirmed metastatic biliary adenocarcinoma.The incidence of PSMs after robotic surgery for biliary malignancies is relatively low, and biliary cancer can be an indication of robotic surgery.


Liu Q.-D.,PLA Second Artillery General Hospital | Chen J.-Z.,PLA Second Artillery General Hospital | Xu X.-Y.,PLA Second Artillery General Hospital | Zhang T.,PLA Second Artillery General Hospital | Zhou N.-X.,PLA Second Artillery General Hospital
World Journal of Gastroenterology | Year: 2012

AIM: To investigate the incidence of clinically detected port-site metastasis (PSM) in patients who underwent robotic surgery for biliary malignancies. METHODS: Using a prospective database, the patients undergoing fully robotic surgery for biliary malignancies between January 2009 and January 2011 were included. Records of patients with confirmed malignancy were reviewed for clinicopathological data and information about PSM. RESULTS: Sixty-four patients with biliary tract cancers underwent robotic surgery, and sixty patients met the inclusion criteria. The median age was 67 year (range: 40-85 year). During a median 15-mo follow-up period, two female patients were detected solitary PSM after robotic surgery. The incidence of PSM was 3.3%. Patient 1 underwent robotic anatomatic left hemihepatectomy and extraction of biliary tumor thrombi for an Klatskin tumor. She had a subcutaneous mass located at the right lateral abdominal wall near a trocar scar. Patient 2 underwent robotic pancreaticoduodenectomy for distal biliary cancer. She had two metachronous subcutaneous mass situated at the right lateral abdominal wall under a same trocar scar at 7 and 26 mo. The pathology of the excised PSM masses confirmed metastatic biliary adenocarcinoma. CONCLUSION: The incidence of PSMs after robotic surgery for biliary malignancies is relatively low, and biliary cancer can be an indication of robotic surgery. © 2012 Baishideng. All rights reserved.


Wang Z.,PLA Second Artillery General Hospital
Surgical laparoscopy, endoscopy & percutaneous techniques | Year: 2013

Since the introduction of Da Vinci robotic surgery, more and more complicated surgeries can now be performed robotically, yet there have been very few on robotic hepatectomy, especially when billiary reconstruction is involved. The video shows our initial experience with an anatomic hepatectomy using Da Vinci surgical robot. In this case, we also conducted billiary reconstruction due to the anatomic abnormality of bile duct, while applying the choledochoscopy. The preoperative diagnosis is primary liver carcinoma, tumor thrombi in bile duct, and hepatitis B. First, the gallbladder was resected, and cystic artery and duct were identified. After opening of the common bile duct above the junction, the choledochoscopy was performed. Tumor thrombi were found in common bile duct and left hepatic duct, and they were all removed. Left branches hepatic artery and portal vein were dissected, ligated, and divided. Thrombi in the left hepatic duct were removed also. After marking the cutting line along the ischemic boarder, liver parenchyma was transected using robotic harmonic scalpel. Branches of ducts were encountered and managed by either direct coagulating or dividing after clipping. The left hepatic vein was visualized, exposed, and divided during hepatectomy. Two T tubes were placed into common hepatic duct and the proximal cutting end of right anterior bile duct which was found to join the left hepatic duct, respectively. The operation went on successfully. The operation time was 410 minutes, the blood loss was 200 mL. The pathologic diagnosis was introductal papillary adenocarcinoma of left hepatic duct. The patient went on well postoperatively and was followed up for 22 months till now. Postoperative computed tomography examination showed no recurrence. Da Vinci-assisted robotic hepatectomy can be performed safely in the hands of experienced hepatobilliary surgeons, and choledochoscopy can be combined for bile duct exploration. With the advantages of Da Vinci robot system, complicated billiary reconstruction can be performed (http://links.lww.com/SLE/A74).


PubMed | PLA Second Artillery General Hospital
Type: Journal Article | Journal: International journal of nursing studies | Year: 2013

The population is ageing globally. Older people are more likely to have chronic diseases and disabilities and have contact with health services. Attitudes of healthcare professionals affect the quality of care provided and individual career preferences.To examine the international research relating to registered and student nurses attitudes towards older people and the potential underpinning variables.A systematic search of 8 databases covering English and Chinese language publications since 2000 was undertaken which identified 25 papers.Reported attitudes towards older people were inconsistent with positive, negative and neutral attitudes being noted across registered and student nurses and appear to be slightly less positive since 2000. A range of variables have been examined as potential predictors of nurses attitudes with age, gender and education level being investigated most frequently but none were consistent predictors. Preference to work with older people and knowledge of ageing appeared to be associated with positive attitudes towards older people.There is a growing need for registered nurses committed to working with older people, however, there is a dearth of well designed studies which investigate both the attitudes of registered and student nurses and the associated factors, and test interventions to inform workforce strategies.


PubMed | PLA Second Artillery General Hospital
Type: Journal Article | Journal: Surgical laparoscopy, endoscopy & percutaneous techniques | Year: 2013

Since the introduction of Da Vinci robotic surgery, more and more complicated surgeries can now be performed robotically, yet there have been very few on robotic hepatectomy, especially when billiary reconstruction is involved. The video shows our initial experience with an anatomic hepatectomy using Da Vinci surgical robot. In this case, we also conducted billiary reconstruction due to the anatomic abnormality of bile duct, while applying the choledochoscopy. The preoperative diagnosis is primary liver carcinoma, tumor thrombi in bile duct, and hepatitis B.First, the gallbladder was resected, and cystic artery and duct were identified. After opening of the common bile duct above the junction, the choledochoscopy was performed. Tumor thrombi were found in common bile duct and left hepatic duct, and they were all removed. Left branches hepatic artery and portal vein were dissected, ligated, and divided. Thrombi in the left hepatic duct were removed also. After marking the cutting line along the ischemic boarder, liver parenchyma was transected using robotic harmonic scalpel. Branches of ducts were encountered and managed by either direct coagulating or dividing after clipping. The left hepatic vein was visualized, exposed, and divided during hepatectomy. Two T tubes were placed into common hepatic duct and the proximal cutting end of right anterior bile duct which was found to join the left hepatic duct, respectively.The operation went on successfully. The operation time was 410 minutes, the blood loss was 200 mL. The pathologic diagnosis was introductal papillary adenocarcinoma of left hepatic duct. The patient went on well postoperatively and was followed up for 22 months till now. Postoperative computed tomography examination showed no recurrence.Da Vinci-assisted robotic hepatectomy can be performed safely in the hands of experienced hepatobilliary surgeons, and choledochoscopy can be combined for bile duct exploration. With the advantages of Da Vinci robot system, complicated billiary reconstruction can be performed (http://links.lww.com/SLE/A74).

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