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Cong T.,Institute of Health and Environmental Medicine | Jin X.,International Center for Liver Disease Treatment | Zhao L.,Chinese PLA General Hospital | Ma L.,The Second Artillery General Hospital of PLA | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: We observed the effects of Masson pine pollen aqueous extracts (MPPAE) on CCl4-induced oxidative damage of the human hepatic cell line L-02. Methods: We created an in vitro model of oxidative liver damage by treating L-02 human hepatic cells with 40 mmol/L CCl4. Effects of different concentrations of MPPAE on cell proliferation, morphology, and change of functional indexes were observed after addition of CCl4. Results: CCl4was toxic to proliferation, cell morphology, and functionality of hepatic cells. It decreased proliferation by 29.3-38.4% and increased AST and ALT activities by 22.3% and 99.2%, respectively. The oxidative stress also disrupted hepatic cell growth and induced pyknosis. Although MPPAE did not prevent decreased proliferation of L-02 cells, the treatment alleviated some CCl4-induced cell morphology changes and inhibited the abnormal rise of ALT (39.8%-70.1%) and AST (14.75-27.25%) activities in a dose dependent manner. A high dose of MPPAE (400 mg/L) ameliorated nucleus deformation to an almost normal appearance. Conclusions: According to our in vitro model, MPPAE specifically prevented the changes in cell morphology and functional injury caused by CCL4 treatment; however, it offered limited protection against damage-induced reduction of proliferation. © 2015, E-Century Publishing Corporation. All rights reserved.

Zhang Z.-P.,Liaoning Medical University | Zhang S.-M.,The Second Artillery General Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Cryopreservation can preserve the activity and function of the in vitro tissues in the greatest degree, which create the enormous push and facilitation effect in medical research and clinical treatment. But in the composite tissue cryopreservation, there also has many problems in the application of cryoprotectant waiting for solve. OBJECTIVE: To review the domestic and foreign research progress in the application of cryoprotectant in tissue cryopreservation METHODS: The PubMed database, Chinese journal full-text database and Wanfang database were searched by the first author for the literatures published from January 2003 to December 2012. The key words were "cryopreservation, cryoprotectant, composite tissues, vitrification, transplantation" in Chinese and English. Totally 143 relevant literatures were collected, and 56 of them were in accordance with the inclusion criteria. RESULTS AND CONCLUSION: Since the beginning of the last century, domestic and overseas experts have made great efforts on the research of tissue cryopreservation through a lot of fundamental experimental researches of animal cells and tissues, and variety of cryopreservation techniques and various cryoprotectants were applied and obtained a certain achievements, it found that the application of cryoprotectant played a crucial role in tissue cryopreservation. Cryopreservation is widely applied in some domains, such as tissue engineering, reproductive medicine and transplantation field. But at present, the application of cryoprotectant in composite tissue cryopreservation is still under studying in laboratory. The types and concentrations of cryoprotectant, as well as the addition and elution process can directly determine the ultimate success of cryopreservation.

Wang X.,Shanghai University | Chang X.,Yancheng City First Peoples Hospital | Zhuo G.,The Second Artillery General Hospital of PLA | Sun M.,Shanghai University | Yin K.,Shanghai University
International Journal of Molecular Sciences | Year: 2013

Tumors can induce the generation and accumulation of immunosuppressive cells such as myeloid-derived suppressor cells in the tumor microenvironment, contributing to tumor immunological escapes. Many studies have demonstrated that multiple factors could induce myeloid precursor cells into myeloid-derived suppressor cells, not dendritic cells. In our study, we found that tumor supernatants could induce the generation of myeloid-derived suppressor cells by disturbing the development of dendritic cells. Twist and miR-34a may regulate the effect of tumor cells inducing myeloid-derived suppressor cells via TGF-β and/or IL-10. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Wu C.,Soochow University of China | Xie X.,Soochow University of China | Cui F.,Soochow University of China | Jiao Y.,Soochow University of China | And 6 more authors.
Genes and Genomics | Year: 2014

Radon exposure has been linked to lung carcinogenesis in both human and animal studies. The identification of sequential changes in DNA methylation during tumour progression and the elucidation of their interplay with genetic changes will broaden our molecular understanding of this disease. Rats were exposed to 120 or 400 working level months (WLM) of radon, lung pathological changes were examined by haematoxylin and eosin staining, lung single cell suspension cell cycles were detected by flow cytometry, lung cell cycle regulated gene (Cdkn2a, P53, Cdk4/2, Mdm4/2 and Rb1 genes) expression was quantified by real-time PCR and methylation of CpG islands in the promoters of cell cycle-regulated genes were detected by bisulfite sequencing PCR. The alveolar walls of rat lungs after exposured to radon exhibited papillae and the lung bronchial epithelial cells stained positively for proliferating cell nuclear antigen. The bronchial epithelial cells displayed some hyperplasia after challenged by 400 WLM of radon. Moreover, G1 arrest decreased; Rb1, Mdm2/4, and Cdk2/4 expression decreased and Cdk2 was demethylated at the second and sixth CpG loci from base pairs 3092704 to 3092953 of chromosome 7. Cdk2 demethylation may be applicable as a biomarker of early lung damage that was induced by radon and other environmental carcinogens. © 2014, The Genetics Society of Korea and Springer-Science and Media.

Wen D.,Yanshan University | Bian Z.,Yanshan University | Li Q.,The Second Artillery General Hospital of PLA | Wang L.,The Second Artillery General Hospital of PLA | And 2 more authors.
Clinical Neurophysiology | Year: 2016

Objective: This study was meant to explore whether the coupling strength and direction of resting-state electroencephalogram (rsEEG) could be used as an indicator to distinguish the patients of type 2 diabetes mellitus (T2DM) with or without amnestic mild cognitive impairment (aMCI). Methods: Permutation conditional mutual information (PCMI) was used to calculate the coupling strength and direction of rsEEG signals between different brain areas of 19 aMCI and 20 normal control (NC) with T2DM on 7 frequency bands: Delta, Theta, Alpha1, Alpha2, Beta1, Beta2 and Gamma. The difference in coupling strength or direction of rsEEG between two groups was calculated. The correlation between coupling strength or direction of rsEEG and score of different neuropsychology scales were also calculated. Results: We have demonstrated that PCMI can calculate effectively the coupling strength and directionality of EEG signals between different brain regions. The significant difference in coupling strength and directionality of EEG signals was found between the patients of aMCI and NC with T2DM on different brain regions. There also existed significant correlation between sex or age and coupling strength or coupling directionality of EEG signals between a few different brain regions from all subjects. Conclusions: The coupling strength or directionality of EEG signals calculated by PCMI are significantly different between aMCI and NC with T2DM. Significance: These results showed that the coupling strength or directionality of EEG signals calculated by PCMI might be used as a biomarker in distinguishing the aMCI from NC with T2DM. © 2015 International Federation of Clinical Neurophysiology.

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