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Chen H.,Second Affiliated Hospital Of Xian Jiaotong University | Wang X.,Second Affiliated Hospital Of Xian Jiaotong University | Bai J.,Second Affiliated Hospital Of Xian Jiaotong University | He A.,Second Affiliated Hospital Of Xian Jiaotong University | He A.,Xi'an Jiaotong University
Oncology Letters | Year: 2017

MicroRNA-495 (miR-495) is a small non-coding RNA encoded by a gene located on chromosome 14 (14q32.31). Its expression is regulated by the transcription factors EF12 and EF47, in addition to promoter methylation status and the fusion oncoprotein mixed-lineage leukemia-AF9. Previous studies suggest that miR-495 is involved in various developmental, immunological and inflammatory processes in healthy tissue, and in the proliferation, invasion, metastasis and drug resistance of cancer cells. The role miR-495 serves in tumors is controversial. miR-495 primarily functions as a tumor suppressor; however, in a number of cases it acts as an oncogene. miR-495 has potential applications as a diagnostic and prognostic marker, and as a therapeutic target for genetic and pharmacological manipulation in the treatment of various diseases. © 2017, Spandidos Publications. All rights reserved.


Yongtao Z.,Second Affiliated Hospital Of Xian Jiaotong University | Kunzheng W.,Second Affiliated Hospital Of Xian Jiaotong University | Jianqiang K.,Qingdao University | Ruiyu L.,Second Affiliated Hospital Of Xian Jiaotong University | Chunsheng W.,Second Affiliated Hospital Of Xian Jiaotong University
Endocrine | Year: 2014

Bone metabolism disorder has been identified to play a vital role in the pathogenesis of glucocorticoid-induced osteoporosis (GIOP). The local renin–angiotensin system (RAS) in bone is newly defined to be closely related to the bone metabolism. However, it is unknown whether the local RAS is involved in GIOP. Adult male New Zealand white rabbits were treated with saline, dexamethasone (DXM) alone, or DXM combined with perindopril. The expression of main RAS components in trabecular bone was examined at mRNA and/or protein levels. Bone metabolism was analyzed using dual-energy X-ray absorptiometry, histomorphometry, biomechanics, biochemical techniques, and quantitative RT-PCR. The expressions of local bone angiotensin II, angiotensin types 1 and 2 receptors, and angiotensin-converting enzyme at mRNA and/or protein levels increased when DXM-induced osteoporosis was present. Whereas, perindopril significantly blocked the activation of the local RAS and partially reversed GIOP. Mineralizing surface, mineral apposition rate, and bone formation rate were decreased by DXM, along with serum osteocalcin being downregulated. These changes were then reversed by the use of perindopril. Osteoclast number, osteoclast surface, and eroded surface increased after the administration of DXM, and urinary deoxypyridinoline was upregulated. These were also inhibited when perindopril was given. Quantitative RT-PCR using RNA isolated from the lumbar vertebrae revealed an increase in the SOST expression and a decrease in the Runx2 expression, whereas the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio and the expression of tartrate resistant acid phosphatase were increased, which were all inhibited by perindopril. The results of this study provide evidence for the role of local RAS is involved in GIOP, and GIOP may be ameliorated by blocking the activation of local RAS in the bone. © 2014, Springer Science+Business Media New York.


Liu B.,Xian Central Hospital | Yuan B.,Xian Central Hospital | Zhang L.,Second Affiliated Hospital Of Xian Jiaotong University | Mu W.,Xian Central Hospital | Wang C.,Xian Central Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

Aim: Emodin was found effective in suppressing proliferation of cancer cells including colorectal cancer (CRC), but the mechanisms were still unclear. This study was aimed to investigate the possible mechanism of emodin’s anti-CRC effects. Methods: Two most frequently used CRC cell lines, SW480 and SW620, were investigated in this study. Serially diluted emodin solutions were used to incubate CRC cells. siRNAs were used to silence the expressions of p38 and Puma respectively. Intracellular ROS production was detected by DCFH-DA staining; proliferation and apoptosis of CRC cells were assessed by MTT assay and Hoechst staining respectively. Western blotting was applied to evaluate the activation of p38/p53/Puma signaling. Results: Both in SW480 and SW620 cells, emodin inhibited proliferation by inducing ROS-mediated apoptosis in a concentration-dependent manner. The p38/p53/Puma signaling was also activated after emodin incubation in a concentration-dependent manner. The ROS scavenger NAC, p38 silencing and Puma silencing impaired the anti-proliferation and apoptosis- inducing effects of emodin. Conclusions: emodin inhibited proliferation of human CRC cells by inducing cell apoptosis by activating ROS/p38/p53/Puma signaling. © 2015, E-Century Publishing Corporation. All rights reserved.


Ma D.L.,National University of Singapore | Qu J.Q.,Second Affiliated Hospital Of Xian Jiaotong University | Goh E.L.K.,National University of Singapore | Goh E.L.K.,Kk Womens And Childrens Hospital | Tang F.R.,National University of Singapore
Frontiers in Neuroanatomy | Year: 2016

In this study, we investigated the reorganized basolateral amygdala (BLA)-subiculum pathway in a status epilepticus (SE) mouse model with epileptic episodes induced by pilocarpine. We have previously observed a dramatic loss of neurons in the CA1-3 fields of the hippocampus in epileptic mice. Herein, we observed a 43-57% reduction in the number of neurons in the BLA of epileptic mice. However, injection of an anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L) into the BLA indicated 25.63% increase in the number of PHA-L-immunopositive terminal-like structures in the ventral subiculum (v-Sub) of epileptic mice as compared to control mice. These data suggest that the projections from the basal nucleus at BLA to the vSub in epileptic mice are resistant to epilepsy-induced damage. Consequently, these epileptic mice exhibit partially impairment but not total loss of context-dependent fear memory. Epileptic mice also show increased c-Fos expression in the BLA and vSub when subjected to contextual memory test, suggesting the participation of these two brain areas in foot shock-dependent fear conditioning. These results indicate the presence of functional neural connections between the BLA-vSub regions that participate in learning and memory in epileptic mice. © 2016 Ma, Qu, Goh and Tang.


Shen Y.,Second Affiliated Hospital Of Xian Jiaotong University | Bai J.,Second Affiliated Hospital Of Xian Jiaotong University | He A.,Second Affiliated Hospital Of Xian Jiaotong University
International Journal of Clinical and Experimental Medicine | Year: 2016

The mammals target protein of rapamycin (mTOR) signaling pathway is crucial to cell survival and proliferation. Alteration of the important components of the mTOR pathway has significant effects on leukemogenesis and resistance to conventional chemotherapy. Thus, we reviewed recent researches of mTOR signaling pathway in acute myeloid leukemia (AML). The inhibitors targeting phosphatidylinositol 3-kinease (PI3K)/AKT/mTOR pathway are applied to many experiments, alone or in combination with cytotoxic drugs. However, some inhibitors alone did not block off the pathway successfully, even lead to reactivation of the pathway because of the existence of feedback mechanism, while the combination of inhibitors or cytotoxic drugs acquire stronger inhibitive effect. Liver kinase B1 (LKB1)/adenosine monophosphate-activated protein kinase (AMPK)/mTOR pathway is known as a tumor suppression axis while some experiments demonstrate it prolongs the proliferation of AML cells. In a word, the role of mTOR pathway in AML is complicated and needs further investigation. © 2016, E-Century Publishing Corporation. All rights reserved.


Zhang F.,Xian Fourth Hospital | Li Z.-Q.,Xian Fourth Hospital | He X.-J.,Second Affiliated Hospital Of Xian Jiaotong University
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Knee osteoarthritis can be treated by total knee arthroplasty. To improve therapeutic effect and promote postoperative recovery, we should take effective measures to improve the joint space and postoperative range of motion. OBJECTIVE: To explore the effectiveness and feasibility of posterior composite release of the knee joint after total knee arthroplasty. METHODS: 118 knee osteoarthritis patients undergoing unilateral total knee arthroplasty in two hospitals from December 2009 to December 2013 were selected, and were randomly divided into control group (59 cases) and observation group (59 cases). After osteotomy during operation, the control group underwent bone removal of conventional condylar hyperplasia. Observation group underwent posterior composite release of the knee joint. Postoperative extension, flexion gap and the time required for postoperative active flexion 90° and 120° were observed in the two groups. Hospital for Special Surgery Knee Score and maximum flexion angle were recorded in 3 months of follow up, and compared between the two groups. RESULTS AND CONCLUSION: Through the statistics and comparison, no significant difference in flexion gap was found between the two groups (P > 0.05). However, significant differences in extension gap, the time for active flexion 90° and 120°and the maximum flexion angle were detectable between the two groups, and above indexes were better in the observation group than in the control group (P < 0.05). Hospital for Special Surgery Knee Score of each index and total score were significantly higher in the observation group than in the control group (P < 0.05). These results suggest that rear joint composite release after total knee arthroplasty has certain validity and feasibility, can effectively improve knee extensor gap and the postoperative range of motion during replacement, but does not impact flexion gap during replacement. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Zhang H.-Y.,Second Affiliated Hospital Of Xian Jiaotong University | Zhang H.-Y.,Yanan University Affiliated Hospital | Cui J.,Yanan University Affiliated Hospital | Zhang Y.,Yanan University Affiliated Hospital | And 3 more authors.
Chinese Medical Journal | Year: 2016

Objective: The purpose of this review is to discuss some critical issues of isoflavones protective against the development of prostate cancer (PCa). Data Sources: Data cited in this review were obtained primarily from PubMed and Embase from 1975 to 2015. Study Selection: Articles were selected with the search terms “isoflavone”, “Phytoestrogen”, “soy”, “genistin”, and “PCa “. Results: Isoflavones do not play an important role on prostate-specific antigen levels reduction in PCa patients or healthy men. The effect of isoflavones on sex hormone levels and PCa risk may be determined by equol converting bacteria in the intestine, specific polymorphic variation and concentrations of isoflavones. The intake of various types of phytoestrogens with lower concentrations in the daily diet may produce synergistic effects against PCa. Moreover, prostate tissue may concentrate isoflavones to potentially anti-carcinogenic levels. In addition, it is noteworthy that isoflavones may act as an agonist in PCa. Conclusions: Isoflavones play a protective role against the development of PCa. However, careful consideration should be given when isoflavones are used in the prevention and treatment of PCa. © 2016 Chinese Medical Journal.


Gu Q.-Y.,Second Affiliated Hospital Of Xian Jiaotong University | Gu Q.-Y.,Affiliated Hospital Of Yanan University | Zhang J.,Second Affiliated Hospital Of Xian Jiaotong University | Feng Y.-C.,Affiliated Hospital Of Yanan University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: The present study aims to investigate the relationship between genetic polymorphisms in HTR3A and HTR3E and diarrhea predominant irritable bowel syndrome (D-IBS) in a Chinese population. Methods: We enrolled 500 D-IBS patients and 500 age- and sex-matched healthy control subjects to detect the genotypes in HTR3A and HTR3B gene by using of PCR-RFLP method. Results: There were significant difference between the D-IBS patients and the health control subjects in the distribution of genotype and allele of rs1062613 in HTR3A gene. As regarding rs62625044 in HTR3E gene, we found there was a significant different between the case and the control group in the distribution of GA genotype and A allele in female but not in male. Conclusion: The present study suggested that there are associations of D-IBS risk with genetic polymorphisms in HTR3A and HTR3E. © 2015, Int J Clin Exp Med. All rights reserved.


Lu T.,Second Affiliated Hospital Of Xian Jiaotong University | Liu C.,Second Affiliated Hospital Of Xian Jiaotong University | Dong J.,Second Affiliated Hospital Of Xian Jiaotong University | Lu M.,Second Affiliated Hospital Of Xian Jiaotong University | And 2 more authors.
International Journal of Computer Assisted Radiology and Surgery | Year: 2016

Purpose: Due to the high screw malposition rate and the potential risk of neurovascular injury in cervical fixation surgeries, guided tools, mainly computer-assisted surgery navigation systems and rapid prototyping drill templates (RPDTs) have increasingly been developed to help surgeons improve screw placement accuracy. Although RPDTs have been used in cervical surgeries for almost 2 decades, no specific review has been performed detailing the state of this technique. Thus, in the current review, we fully discuss the status of applying RPDTs in cervical surgeries. Methods: Studies that tested the accuracy and reliability of RPDTs in guiding cervical screw placements were included in this review. The fabrication workflow and usage of RPDTs, the accuracy and reliability of using RPDTs for screw and plate placement, the advantages and disadvantages of RPDTs and their prospects for future applications as a part of cervical fixation instrumentation are discussed. Results: As the design of RPDTs becomes more rational, the accuracy and reliability of these devices have significantly improved in cervical fixation surgeries. Moreover, RPDTs decrease the intraoperative radiation exposure for surgeons and patients relative to conventional methods. However, some disadvantages also exist. The fabrication of RPDTs is time-consuming, and the time required to learn the related software is long. Conclusion: We believe that because of their merits, the RPDT technique is worth promoting for use in cervical surgeries. However, the time-consuming fabrication workflow and the long period required to learn the related software might limit its widespread use. In the future, the workflow should be simplified to reduce the extra workload for surgeons. Moreover, more clinical studies with high-level evidence are still needed to further test its accuracy and feasibility. © 2016 CARS


Dong J.,Second Affiliated Hospital Of Xian Jiaotong University | Lu M.,Second Affiliated Hospital Of Xian Jiaotong University | Liang B.,Second Affiliated Hospital Of Xian Jiaotong University | Zhai X.,Second Affiliated Hospital Of Xian Jiaotong University | He X.,Second Affiliated Hospital Of Xian Jiaotong University
International Journal of Clinical and Experimental Medicine | Year: 2016

Anterior cervical corpectomy and fusion is an effective procedure in the treatment of cervical spondylotic myelopathy but accompanied with motion loss in the fused levels. A novel hemisphere type of cervical artificial disc and vertebra system (H-ADVS) has been designed to preserve the intervertebral motion function after cervical corpectomy. The aim of study is to evaluate the range of motion of the goat cervical spine model after implanted with this prosthesis in comparison with the fused or intact specimen. A total of 12 male goats were included and divided into experimental and control group with 6 animals in each group. The goats in experimental group were implanted with H-ADVS. All the cervical spine specimens were harvested 6 months after the operation followed by euthanasia. Biomechanics test on the fresh cervical spine specimens was performed. Specimens in experimental group were tested as in vivo H-ADVS group. Specimens in control were tested in sequence as intact group, fusion group and in vitro H-ADVS group. The ranges of motion in flexion-extension, lateral bending and axial rotation of C2-3, C3-4, C4-5 and C2-5 from each group were compared. Significant increased range of motion of C2-3 in most directions in fusion group was observed comparing with the intact group (P < 0.01). The range of motion of operative levels (C3-4, C4-5) in in vivo H-ADVS group and in vitro H-ADVS group were remarkably preserved comparing the intact group (P < 0.01). Significant differences in comparing the range of motion of C2-5 in all the specimens were detected between groups (P < 0.01). Our result suggested that H-ADVS replacement could reduce the range of motion in the upper adjacent level and restore the range of motion of the operative levels comparing with the fusion procedure. In conclusion, H-ADVS provided a new method to restore the range of motion after cervical corpectomy. However, improvements on the design of this prosthesis should be continued. © 2016, E-Century Publishing Corporation. All rights reserved.

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