San Miguel de Tucumán, Argentina
San Miguel de Tucumán, Argentina

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Castagnaro A.P.,Seccion Biotecnologia | Filippone M.P.,Seccion Biotecnologia | Noguera A.S.,Seccion Biotecnologia | Perera M.F.,Seccion Biotecnologia | And 20 more authors.
BAG - Journal of Basic and Applied Genetics | Year: 2011

In 1994, a group whose aims were both scientific research and technological development was set up. This team was inspired by the increasing demand of the agro-industrial sector in the North West of Argentina. Besides financial considerations inherent to the productive process, social and environmental issues were borne in mind when research had to be carried out. The conceptual premises of scientific work were the study, preservation and a judicious use of biodiversity; equally important was the relentless search for phytosanitary management alternatives which were friendly to both humans and the environment. Thus, in 1997, the first National Program of Strawberry Genetic Breeding in Argentina started at INSIBIO (CONICET-UNT), Tucumán. This Institute coordinated this program on a national level. Thanks to an agreement between INSIBIO and the Estación Experimental Agroindustrial Obispo Colombres (EEAOC), in 2002, the EEAOC's Biotechnology Department, a Unit Associated to INSIBIO, was founded. One of the main goals of this department was both to develop and adapt technologies to support institutional ongoing programs which intended not only to increase productivity but also to confer more sustainability to those agro-industries related to sugar and alcohol, soybean and citrus. And most importantly, a new scientific and technological team constituted by professionals working with other groups from different research centers nationwide came into existence. Their common goal has been the improvement of agro-industrial productive processes in order to help build a society which is fair and equitable for both the present and the future.


Mamani A.,Instituto Superior Of Investigaciones Biologicas Insibio | Filippone M.P.,Seccion Biotecnologia | Grellet C.,Instituto Superior Of Investigaciones Biologicas Insibio | Welin B.,Seccion Biotecnologia | And 2 more authors.
Molecular Plant-Microbe Interactions | Year: 2012

In an incompatible interaction between Colletotrichum fragariae and strawberry plants, the accumulation of phenolic compounds in plant leaves was observed. A particularly abundant penta-esterified ellagitannin that accumulated in response to pathogen attack was identified as 1-0-galloyl-2,3;4,6-bis- hexahydroxydiphenoyl-β-D-glucopyranose (HeT) by mass spectroscopy and nuclear magnetic resonance. Foliar application of purified HeT prior to inoculation with a virulent pathogen was shown to increase resistance toward C. acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. The induced resistance in strawberry was associated with a rapid oxidative burst, callose deposition, a transient increase of salicylic acid in phloem, and induction of gene expression responsive to salicylic acid. Results obtained suggested that HeT could be a common plant defense response molecule capable of inducing pathogen resistance in different plant species. © 2012 The American Phytopathological Society.


Ontivero M.,Seccion Biotecnologia | Zamora G.M.,Instituto Superior Of Investigaciones Biologicas Insibio | Salazar S.,Instituto Nacional de Tecnologia Agropecuaria | Salazar S.,National University of Tucuman | And 2 more authors.
Genome | Year: 2011

Actin depolymerizing factors (ADFs) have been recently implicated in plant defense against pathogenic fungi, associated with the cytoskeletal rearrangements that contribute to establish an effective barrier against fungal ingress. In this work, we identified a DNA fragment corresponding to a part of a gene predicted to encode an ADF-like protein in genotypes of Fragaria ananassa resistant to the fungus Colletotrichum acutatum. Bulked segregant analysis combined with AFLP was used to identify polymorphisms linked to resistance in hybrids derived from the cross between the resistant cultivar 'Sweet Charlie' and the susceptible cultivar 'Pájaro'. The sequence of one out of three polymorphic bands detected showed significant BLASTX hits to ADF proteins from other plants. Two possible exons were identified and bioinformatic analysis revealed the presence of the ADF homology domain with two actin-binding sites, an N-terminal phosphorylation site, and a nuclear localization signal. In addition to its possible application in strawberry breeding programs, these finding may contribute to investigate the role of ADFs in plant resistance against fungi. © 2011 Published by NRC Research Press.


Chalfoun N.R.,CONICET | Grellet-Bournonville C.F.,CONICET | Martinez-Zamora M.G.,CONICET | Diaz-Perales A.,Technical University of Madrid | And 3 more authors.
Journal of Biological Chemistry | Year: 2013

In this work, the purification and characterization of an extracellular elicitor protein, designated AsES, produced by an avirulent isolate of the strawberry pathogen Acremonium strictum, are reported. The defense eliciting activity present in culture filtrates was recovered and purified by ultrafiltration (cutoff, 30 kDa), anionic exchange (Q-Sepharose, pH 7.5), and hydrophobic interaction (phenyl-Sepharose) chromatographies. Two-dimensional SDS-PAGE of the purified active fraction revealed a single spot of 34 kDa and pI 8.8. HPLC (C2/C18) and MS/MS analysis confirmed purification to homogeneity. Foliar spray with AsES provided a total systemic protection against anthracnose disease in strawberry, accompanied by the expression of defense-related genes (i.e. PR1 and Chi2-1). Accumulation of reactive oxygen species (e.g. H 2O2 and O2 .-) and callose was also observed in Arabidopsis. By using degenerate primers designed from the partial amino acid sequences and rapid amplification reactions of cDNA ends, the complete AsES-coding cDNA of 1167 nucleotides was obtained. The deduced amino acid sequence showed significant identity with fungal serine proteinases of the subtilisin family, indicating that AsES is synthesized as a larger precursor containing a 15-residue secretory signal peptide and a 90-residue peptidase inhibitor I9 domain in addition to the 283-residue mature protein. AsES exhibited proteolytic activity in vitro, and its resistance eliciting activity was eliminated when inhibited with PMSF, suggesting that its proteolytic activity is required to induce the defense response. This is, to our knowledge, the first report of a fungal subtilisin that shows eliciting activity in plants. This finding could contribute to develop disease biocontrol strategies in plants by activating its innate immunity. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.


Debes M.A.,National University of Tucuman | Arias M.E.,National University of Tucuman | Grellet-Bournonville C.F.,CONICET | Wulff A.F.,University of Buenos Aires | And 3 more authors.
American Journal of Botany | Year: 2011

Premise of the study: Duchesnea indica is a wild strawberry-like species that has red fruits. In a recent survey in the highlands of Tucum á n (Argentina), a plant of D. indica with white fruits was discovered. The aim of this study was to investigate whether the white-fruited character was due to a phenotypic or genotypic change. The stability and heritability of the character and the expression of genes involved in anthocyanins synthesis were studied and compared with red-fruited genotypes. This study contributes to understanding the molecular basis of some factors involved in fruit pigmentation, a horticulturally and taxonomically important trait. Methods: Stability and heritability of the white-fruited character were evaluated in plants obtained by asexual propagation or by sexual crosses between the white- and red-fruited genotypes. Asexual multiplications were carried out by stolon rooting and sexual multiplications by germination of achenes obtained from crosses. The expression level of the genes involved in the synthesis and regulation of the anthocyanins pathway (CHS, F3H, DFR, ANS, and MYB10) were evaluated by RT-PCR using specific primers. Key results: Plants with the white-fruited character always yielded white-fruited progeny when propagated asexually, whereas in sexually propagated plants fruit color depended on the mother. Red-fruited mothers yielded red-fruited progeny, and whitefruited mothers yielded fruits ranging from dark pink to white. Molecular analysis suggested that the white-fruited character was due to the low expression of the ANS gene. Conclusions: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or downregulation of the ANS gene. © 2011 Botanical Society of America.


Zamora M.G.M.,CONICET | Bournonville C.G.,CONICET | Castagnaro A.P.,Seccion Biotecnologia | Ricci J.C.D.,CONICET
Functional Plant Biology | Year: 2012

The identification of a full length cDNA encoding an endo-β-1,3- glucanase (FaOGBG-5) from strawberry (Fragaria×ananassa Duch) is reported. The analysis of the deduced amino acid sequence of FaOGBG-5 showed that it shares typical structural features and a high degree of identity with other plant β-1,3-glucanases of the class I. The expression of FaOGBG-5 in plants infected with a virulent isolate of Colletotrichum acutatum and an avirulent isolate of Colletotrichum fragariae was examined. Induction of expression was observed with both pathogens but exhibited a delayed high expression with the virulent one. Additionally, the accumulation of FaOGBG-5 transcripts was also observed after treatments with the stress related hormones salicylic acid and ethylene. Results obtained suggest that the β-1,3-glucanase encoded by FaOGBG-5 may be implicated in plant defence against biotic and abiotic stress. © 2012 CSIRO.

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