NORTH BILLERICA, MA, United States
NORTH BILLERICA, MA, United States

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Patent
Seahorse Bioscience, Inc. | Date: 2015-09-18

A method of analyzing cells disposed in media within a vessel includes the steps of providing a vessel having an original volume of media about the cells, reducing the original volume of media about at least a portion of the cells to define a reduced volume of media, and analyzing a constituent related to the cells within the reduced volume of media. An apparatus for analyzing cells includes a stage adapted to receive a vessel holding cells and a volume of media, a plunger adapted to receive a barrier to create a reduced volume of media within the vessel including at least a portion of the cells, the barrier adapted for insertion into the vessel by relative movement of the stage and the plunger, and a sensor in sensing communication with the reduced volume of media, wherein the sensor is configured to analyze a constituent disposed within the reduced volume.


Grant
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 224.84K | Year: 2014

Not Available


The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two chemical compounds: a mitochondrial uncoupler and an ATP synthase inhibitor. In one embodiment, the metabolic energy generating activity of the mitochondrial respiration pathway is determined by measuring the rate of oxygen consumption by the living cells, and the metabolic energy generating activity of the glycolysis pathway is determined from a measurement of extracellular acidification caused by secretion of protons from the cell. Other embodiments are related to an apparatus for determining a metabolic potential of a cell sample in a well of a multiwell plate.


Patent
Seahorse Bioscience, Inc. | Date: 2014-03-04

A method of analyzing cells disposed in media within a vessel includes the steps of providing a vessel having an original volume of media about the cells, reducing the original volume of media about at least a portion of the cells to define a reduced volume of media, and analyzing a constituent related to the cells within the reduced volume of media. An apparatus for analyzing cells includes a stage adapted to receive a vessel holding cells and a volume of media, a plunger adapted to receive a barrier to create a reduced volume of media within the vessel including at least a portion of the cells, the barrier adapted for insertion into the vessel by relative movement of the stage and the plunger, and a sensor in sensing communication with the reduced volume of media, wherein the sensor is configured to analyze a constituent disposed within the reduced volume.


An apparatus including a plurality of wells for conducting analysis of three-dimensional cell samples (e.g., tissue samples) and methods for experimenting with a three-dimensional sample. A removable insert for use with the apparatus enables plunger-driven perfusion of the three-dimensional sample.


Patent
Seahorse Bioscience, Inc. | Date: 2015-08-19

Devices and methods that measure one or more properties of a living cell culture that is contained in liquid media within a vessel, and typically analyzes plural cell cultures contained in plural vessels such as the wells of a multiwell microplate substantially in parallel. The devices incorporate a sensor that remains in equilibrium with, e.g., remains submerged within, the liquid cell media during the performance of a measurement and during addition of one or more cell affecting fluids such as solutions of potential drug compounds.


Patent
Seahorse Bioscience, Inc. | Date: 2014-01-08

Devices and methods that measure one or more properties of a living cell culture that is contained in liquid media within a vessel, and typically analyzes plural cell cultures contained in plural vessels such as the wells of a multiwell microplate substantially in parallel. The devices incorporate a sensor that remains in equilibrium with, e.g., remains submerged within, the liquid cell media during the performance of a measurement and during addition of one or more cell affecting fluids such as solutions of potential drug compounds.


Patent
Seahorse Bioscience, Inc. | Date: 2014-05-21

A method and apparatus for managing curation of the content of a network-based shared folder may comprise: creating, via a user computing device having Internet access, an originating curator created folder; selecting, via the user computing device, originating curator selected content and incorporating the originating curator selected content into the originating curator created folder; designating, via the user computing device, at least one other to act as curator of the content kept in the originating curator created folder to the same extent as the originating curator, for the content kept in the originating curator created folder at the time of the designation of the at least one other curator and up until the at least one other curator is deleted as a curator for the folder. The method and apparatus may allow the deleted curator access to the content at the time the deleted curator is deleted.


Patent
Seahorse Bioscience, Inc. | Date: 2015-06-02

A multiwell microplate for holding liquid samples, and a method of use thereof. The multiwell microplate includes a frame defining a plurality of wells disposed in a single column, each well having an opening with a length l_(1). A moat is disposed about the plurality of wells. A plurality of walls traverses the moat, the walls defining a plurality of compartments, each compartment having a length l_(2 )selected from a range of greater than l_(1 )and less than 6l_(1). A multiwell microplate carrier includes a body defining a plurality of regions configured to hold a plurality of multiwell microplates in parallel, each multiwell microplate defining a single column of wells, and each of the regions defining a plurality of openings that are adapted to mate with the single columns of wells.


Patent
Seahorse Bioscience, Inc. | Date: 2011-05-18

Table 5 demonstrates that, as expected, cell proliferation results in an increase in oxygen consumption and the rate of extracellular acidification. Previous studies have shown that stimulation of transmembrane receptors often causes a rapid increase in extracellular acidification rate, resulting primarily from acute activation of ion exchange pumps. In this experiment, the prototype device was used to detect a change in extracellular acidification rate following treatment of cells with a receptor agonist. Chinese hamster ovary (CHO) cells were transfected to over-express the muscarinic receptor subtype m3. The prototype device described in Example 1 was then used to monitor O_(2) consumption, CO_(2) production, and extracellular acidification rates, following treatment with the well-known, general acetylcholine receptor agonist, Carbachol. Materials and Methods: Cell culture reagents were obtained from Gibco BRL (Grand Island, NY). Carbachol was purchased from Sigma Chemical Co. (St. Louis, MO). Biearbonate-free DMEM medium was obtained from Specialty Media (Phillipsburg, NJ). Polycarbonate membrane snapwells (12 mm diameter, 3 m pore size) were obtained from Corning (Corning, NY). CHO cells expressing m3-muscarinic receptors (CHO-M3 cells) were obtained from the American Type Tissue Culture (ATCC; Manassas, VA). Cells were cultured in Hams F-12 medium supplemented with 10% fetal bovine serum (Hyclone), 1% GlutaMax and 0.1 % Gentamicin and were maintained in a 5% CO_(2) incubator. Cells were subcultured when they reached 80% confluency. CHO-M3 cells were seeded at a density of 2x10^(5) onto a snapwell 24 hours prior to use. Immediately prior to testing, cells on snapwells were switched to bicarbonate-free DMEM medium combined with 3.7 g/l NaCl to maintain osmolarity (medium pH 7.4 - 7.5). Protocol Description: Probes were calibrated immediately prior to testing. The bottom of the test vessel was filled with bicarbonate-tree medium. The snapwell was removed from a 5% CO2 incubator, and the regular growth medium (Hams F-12) was replaced with bicarbonate-free DMEM medium. Thereafter, the snapwell was placed into the test vessel. Bicarbonate-free medium was pipetted onto the top of the snapwell, and the cover piece of the test vessel was placed gently on top of the snapwell and screwed into place, compressing the assembly. The probe software was started, and the pH, CO_(2) and O_(2) analytes were measured over

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