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Soegaon, India

Bhosale V.N.,search Center Yeshwant Mahavidyalaya | Khansole G.S.,Dabnarts And Science College | Angulwar J.J.,Dayanand Science College | Choudhare S.S.,Sd College
Der Pharma Chemica | Year: 2015

An environmentally benign tandem Knowvengel-Michael reaction of ethylacetoacetae, hydrazine hydrate,malononitrile and substituted aromatic aldehyde for the synthesis of 6-amino -1,4-dihydro -3- methyl -1,4- phenyl Pyrano [2,3-c] Pyrazoles .

Gupta V.,Maharishi Markandeshwar University | Verma M.,Maharishi Markandeshwar University | Gulati N.,Sd College
Journal of Uncertain Systems | Year: 2016

We prove the existence and uniqueness of common fixed point for a sequence of mapping, considering each pair of mapping satisfies the contractive condition on fuzzy metric space. We also prove same for generalized fuzzy metric spaces. We give the lemma to prove the main result in generalized fuzzy metric space. In this paper we extend the theorem of Gajic [5] in fuzzy metric space as well as in generalized fuzzy metric space for sequence of mappings. We give example to explain our main result. © 2016 World Academic Press, UK. All rights reserved.

Nishanth S.K.,Central Tuber Crops Research Institute | Nambisan B.,Central Tuber Crops Research Institute | Dileep C.,Sd College
Peptides | Year: 2014

In continuation of our search for new bioactive secondary metabolites from Bacillus cereus associated with entomopathogenic nematode (EPN), three cyclic dipeptides (CDPs), cyclo(l-Leu-d-Arg) (1), cyclo(2-hydroxy-Pro-l-Leu) (2), and cyclo(l-Val-l-Pro) (3) were purified from the ethyl acetate extract of B. cereus. The chemical structure of the compounds was identified by 1D, 2D NMR and HR-ESI-MS. Cyclo(l-Leu-d-Arg) recorded best antifungal activity and the highest activity was recorded against Cryptococcus neoformans (1 μg/mL), which is better than the standard antifungal agent amphotericin B. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used for finding cell proliferation inhibition and cyclo(l-Leu-d-Arg) recorded significant activity against breast cancer cell line (MDAM-B231) (IC50 value: 25 μM) and the three cyclic dipeptides recorded no toxicity against normal human cell (fore skin (FS) normal fibroblast) up to 50 μM except cyclo(l-Val-l-Pro). Cyclo(l-Leu-d-Arg) induced significant morphological changes and DNA fragmentation associated with apoptosis in MDAM-B231 cells by acridine orange/ethidium bromide staining and flow cytometry analysis. Out of three cyclic dipeptides tested only cyclo(2-hydroxy-Pro-l-Leu) recorded significant antioxidant activity. The hydroxyl radical scavenging activity of cyclo(2-hydroxy-Pro-l-Leu) is greater than BHA, the standard antioxidant agent. Cyclo(l-Leu-d-Arg) was isolated for the first time from a natural source with a d-arginine residue. To the best of our knowledge, this is the first time that the bioactivity of the isolated cyclic dipeptides is reported against medically important fungi and cancer cells. This study is a significant contribution to the knowledge of cyclo(l-Leu-d-Arg) from B. cereus as potential sources of new drugs in the pharmacological industry, especially as potent antifungal and anticancer agent. © 2013 Elsevier Inc.

Kumar S.N.,Central Tuber Crops Research Institute | Sreekala S.R.,Central Tuber Crops Research Institute | Chandrasekaran D.,Sd College | Nambisan B.,Central Tuber Crops Research Institute | Anto R.J.,Integrated Cancer Research Program
PLoS ONE | Year: 2014

The rhabditid entomopathogenic nematode associated Bacillus cereus and the antifungal compounds produced by this bacterium were evaluated for their activity in reducing postharvest decay of peanut kernels caused by Aspergillus species in in vitro and in vivo tests. The results showed that B. cereus had a significant effect on biocontrol effectiveness in in vitro and in vivo conditions. The antifungal compounds produced by the B. cereus were purified using silica gel column chromatography and their structure was elucidated using extensive spectral analyses. The compounds were identified as diketopiperazines (DKPs) [cyclo-(L-Pro-Gly), cyclo(L-Tyr-L-Tyr), cyclo-(L-Phe-Gly) and cyclo(4-hydroxy-L-Pro-L-Trp)]. The antifungal activities of diketopiperazines were studied against five Aspergillus species and best MIC of 2 μg/ml was recorded against A. flavus by cyclo(4-hydroxy-L-Pro-L-Trp). To investigate the potential application of cyclo(4-hydroxy-L-Pro-L-Trp) to eliminate fungal spoilage in food and feed, peanut kernels was used as a food model system. White mycelia and dark/pale green spores of Aspergillus species were observed in the control peanut kernels after 2 days incubation. However the fungal growth was not observed in peanut kernels treated with cyclo(4-hydroxy-L-Pro-L-Trp). The cyclo(4-hydroxy-L-Pro-L-Trp) was nontoxic to two normal cell lines [fore skin (FS) normal fibroblast and African green monkey kidney (VERO)] up to 200 μg/ml in MTT assay. Thus the cyclo(4-hydroxy-L-Pro-L-Trp) identified in this study may be a promising alternative to chemical preservatives as a potential biopreservative agent which prevent fungal growth in food and feed. To the best of our knowledge, this is the first report demonstrating that the entomopathogenic nematode associated B. cereus and cyclo(4-hydroxy-L-Pro-L-Trp) could be used as a biocontrol agents against postharvest fungal disease caused by Aspergillus species. © 2014 Kumar et al.

Gupta V.,Maharishi Markandeshwar University | Kanwar A.,Maharishi Markandeshwar University | Gulati N.,Sd College
Smart Innovation, Systems and Technologies | Year: 2016

The present study is devoted to use the notion of joint common limit (shortly, JCLR property) for coupled maps and utilize this concept to prove common coupled fixed point theorem on fuzzy metric space. In this paper, we also prove some fixed point theorems using the concept CLR property, E.A property and integral type contractive condition in fuzzy metric space. Illustrative examples supporting main results have been given. © Springer India 2016.

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