Scientific Veterinary Institute of Serbia

Belgrade, Serbia

Scientific Veterinary Institute of Serbia

Belgrade, Serbia
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Samanc H.,University of Belgrade | Danijela K.,University of Belgrade | Jovanovic M.,University of Belgrade | Vujanac I.,University of Belgrade | And 4 more authors.
Acta Veterinaria | Year: 2010

The aim of this study was to estimate the association between body condition and fatty liver in high-yielding dairy cows. One hundred dry Holstein cows were selected. Cows were scored once for body condition during the dry period, Puerperium and month 2 of lactation, according to the system provided by Elanco Animal Health Buletin Al 8478. Body condition loss was determined as marked if loss was over 0.7 points between two consecutive phases of cycles and over 1.5 points between Puerperium and month 2 of lactation. Liver tissue samples were taken 12 d after calving and tested for lipid content. 50% of cows had mild (3.92 ± 3.33% fat), 33% moderate (19.28 ± 5.18% fat), and 17% severe fatty liver (36.21 ± 4.55% fat). The mean body condition scores were 3.79 ± 0.55 (dry period), 3.18 ± 0.34 (Puerperium) and 2.90 ± 0.29 (month 2 of lactation). Marked body condition loss from the dry period to Puerperium had 38% of cows, 20% from dry period to month 2 of lactation and 8% from Puerperium to month 2 of lactation. Body condition scores out of the physiological range had 39% dry cows, 49 % cows in puerperium and 9% cows at month 2 of lactation. After dividing cows into groups according to fatty liver degree, it was shown that only cows with severe fatty liver were obese during the dry period and that 76.47% of cows from this group had marked condition loss from the dry period to puerperium, 47.06% from dry period to month 2 of lactation and 23.53% from puerperium to month 2 of lactation. These indicate that in cows with severe fatty liver lipomobilisation is intensive and starts around calving, before milk production enhacement. Our results indicate a strong association between body condition and fatty liver in cows, which can be estimated only when body condition is analyzed in details. Besides, our results clearly indicate that body condition is not the only etiological factor that leads to fatty liver in dairy cows.

Stijepic M.,Higher Medical School | Glusac J.,Higher Medical School | Durdevic-milosevic D.,Anahem Laboratory | Pesic-Mikulec D.,Scientific Veterinary Institute of Serbia
Romanian Biotechnological Letters | Year: 2013

The main objectives of this study were (1) to produce control cow's yoghurt that will serve as basis of comparison for produced cow's and soy yoghurts with added probiotic cultures and inulin as prebiotic, (2) to determine trend of change of pH values in yoghurt during the fermentation, (3) to investigate the changes of some physicochemical properties of experimental designed yoghurts during the refrigerated storage. The drop in pH during fermentation was faster in the cow's milk than in soymilk. During the storage at 4 °C the pH value of the fermented products remained mostly unchanged compared to control sample under same condition of storage. No great differences are noticed among the viscosities and water holding capacity (WHC) after 1st and 20th day of storage. Only after 10th day of storage were noted increasing in WHC in all of produced yoghurt sample, except fermented cow's milk with 3% inulin addition. Statistical analysis of experimental results showed no difference in syneresis among observed samples and control sample. This research proves that there are serious confirmations those supporting develop of new soy yoghurt formulation. Stability during storage is important characteristic what is proved observing physicochemical characteristics of produced samples of soy yoghurt. © 2013 University of Bucharest.

Ivanovic S.,Scientific Veterinary Institute of Serbia | Baltic M.Z.,University of Belgrade | Zujovic M.,Serbian Institute for Animal Husbandry | Tomic Z.,Serbian Institute for Animal Husbandry | And 2 more authors.
African Journal of Biotechnology | Year: 2011

In this study, chemical composition, colour and sensory characteristics of Bunte Deutsche Edelziege (BDE) and Balkan goat meat were examined. Both species of goats were fed and raised under semiintensive system of management. Chemical composition (moisture, ash, fat and protein content) and pH value were measured by standard methods and the colour was determined instrumentally. Moisture, protein, fat, ash and colour characteristics were not significantly different in both species of goats. Sensory evaluation revealed that the odour intensity was significantly (P < 0.01) higher in the meat of BDE, whereas, tenderness, softness, taste acceptability and overall impression were significant (P < 0.001) in the meat of Balkan goat. Therefore, it can be concluded that the meat of Balkan goat has better sensory characteristics in relation to the meat of BDE, although chemical composition and colour characteristics were found to be similar in both meat. © 2011 Academic Journals.

Pavlovic I.,Scientific Veterinary Institute of Serbia | Savic B.,Scientific Veterinary Institute of Serbia
Journal of Parasitic Diseases | Year: 2017

The result of helminthological examination of 28 hedgehogs died at traffic accident and 12 samples of feces collected from pets hedgehogs during 2013–2015 were presented. Three species of helminths were found: Crenosoma striatum, Hymenolepis erinacei and Aonchotheca erinacei. This is the first research of parasitic fauna of hedgehogs in Serbia. © 2016, Indian Society for Parasitology.

Milicevic D.R.,Serbian Institute of Meat Hygiene and Technology | Jovanovic M.,University of Belgrade | Matekalo-Sverak V.F.,Serbian Institute of Meat Hygiene and Technology | Radicevic T.,Serbian Institute of Meat Hygiene and Technology | And 2 more authors.
Archive of Oncology | Year: 2011

Background: Toxicological investigations of tissues of normally slaughtered chickens were carried out to provide preliminary evaluation of the incidence of OTA in chicken tissues (n=90). Majority of tissue samples were not found to contain measurable amounts of OTA, while in general, the OTA levels found in the analyzed tissue were low. Methods: The presence of OTA in tissue samples was determined by HPLC-FL after liquid-liquid extraction procedure. Method validation was performed according to the Commission Decision 2002/657/EC. Results: Of the 90 liver, kidney and gizzard samples originating from chicken farms located in the different agricultural areas of Serbia, OTA was reported in 23 (38.33%), 17 (28.3%) and 16 (26.6%) samples, respectively, with levels ranging from 0.14 to 3.9 ng/g in liver, 0.1 to 7.02 ng/g in kidneys and 0.25 to 9.94 ng/g in gizzard. None of the tissue samples contained more than the maximum level (10 ng/g) recommended by the European Commission. Conclusion: Low OTA results also suggested that chicken meat available in the retail market is unlikely to pose an adverse health risk to the consumers in respect to OTA toxicity. © 2011, Oncology Institute of Vojvodina, Sremska Kamenica.

Simin S.,University of Novi Sad | Spasojevic Kosic L.,University of Novi Sad | Kuruca L.,University of Novi Sad | Pavlovic I.,Scientific Veterinary Institute of Serbia | And 2 more authors.
Parasites and Vectors | Year: 2014

Findings: In March 2013, a female hunting dog was presented to a veterinary clinic in Novi Sad, Serbia, for examination of a chronic skin problem. The dog had no history of respiratory or cardiovascular diseases. Faecal and urine samples were collected and examined for the presence of parasite ova/cysts. A modified Baermann test detected 8.8 larvae per gram of faeces. Based on their overall body length (mean 381.7 ± 15.9 μm; range from 342.5 to 404.3 μm; n = 12) and characteristic tail morphology, they were identified as the first-stage larvae of A. vasorum.Background: Angiostrongylus vasorum is a cardiopulmonary canine nematode, potentially fatal to its host. In the last decade, there has been an increasing number of autochthonous cases in areas previously considered non-endemic. However, information about the parasite's occurrence and distribution among Central and Eastern (Southeastern) European countries are scarce. This paper reports the first recorded case of autochthonous A. vasorum infection in a hunting dog from Serbia.Conclusions: The spread of A. vasorum to the southeast of Europe is further confirmed after finding autochthonous infected dog from Serbia. Therefore, veterinary professionals in Serbia should consider A. vasorum in differential diagnosis of dogs. © 2014 Simin et al.; licensee BioMed Central Ltd.

Ivanovic S.D.,Scientific Veterinary Institute of Serbia | Stojanovic Z.M.,Serbian Environmental Protection Agency SEPA | Baltic M.Z.,University of Belgrade | Pisinov B.P.,Scientific Veterinary Institute of Serbia | Nesic K.D.,Scientific Veterinary Institute of Serbia
Hemijska Industrija | Year: 2013

Chemical composition, pH value, fatty acids profile, cholesterol content, color and sensory analysis of pork meat from Duroc×Yorkshire (D×Y), Duroc×Yorkshire×wild boar (D×Y×WB) crossbreeds and wild boars (WB) was investigated. Samples for all tests were taken from m. longissimus dorsi. The chemical composition and pH value were tested by ISO methods. Fatty acid and cholesterol determination was performed by gas chromatography with external standard. The color was determined instrumentally using the thristimulus colouri-meter. The overall sensory quality (appearance, texture and smell) of samples of raw meat was evaluated. A scoring system was used in the evaluation of the results. Statistically significant differences (p < 0.05) were found in the chemical composition (moisture, fat, protein and ash) and pH values between each of the examined groups, as well as fatty acids and cholesterol content among all the examined groups. Measurments of the colour of meat from all three groups showed that the L*, a*, b*, Chroma and Hue angle were also statistically significantly different (p < 0.01).

Milicevic D.R.,Serbian Institute of Meat Hygiene and Technology | Jovanovic M.,University of Belgrade | Juric V.B.,University of Novi Sad | Petrovic Z.I.,Serbian Institute of Meat Hygiene and Technology | Vukovic D.Z.,Scientific Veterinary Institute of Serbia
Archive of Oncology | Year: 2010

Background: In order to ensure the safety of consumers in Serbia, the toxic elements' (Cd, Hg, As, Pb) content in swine kidneys collected from three different sites in Serbia (n=90), were determined by atomic absorption spectrometry. Also, in order to find information on the effects of accumulation of toxic elements in swine kidneys, histopathological examination of kidneys was carried out. Methods: Determination of toxic elements (Cd, Hg, As, Pb) in swine kidneys was performed by atomic absorption spectrometry. For microscopic examination, kidney samples were fixed in 10% neutral buffered formalin and absolute alcohol for 5 to 7 days, processed by routine methods, sectioned at 5-8 μm, and stained with hematoxylin and eosin (HE) for light microscopy. Results: The presence of mercury was found in 33.3% of kidney samples in the range of 0.005-0.055 mg/kg, while presence of cadmium was found in less degree (27.7%) but in higher content (0.05-1,23 mg/kg). The presence of arsenic was found only in one sample, while presence of lead was not found. The metal-to-metal correlation analysis supported the theory that there were different sources of contamination. Histopathological examination of kidneys confirms tubulopathies with edema and cell vacuolization. In addition, hemorrhages and necrosis of proximal kidney tubules' cells were found. Conclusion: This study shows the presence of toxic elements in pigs butchered in Serbia at levels comparable to those reported in other countries., and consequently do not represent any concern from a consumer safety point of view. The lack of strong correlation between histopathological changes and incidence of toxic elements found in our trial may explain the possible synergism among toxic elements and other nephrotoxic compounds, which enhances the toxicity, especially in cases of low contamination. © 2010, Oncology Institute of Vojvodina, Sremska Kamenica.

Pavlovic I.,Scientific Veterinary Institute of Serbia | Ivanovic S.,Scientific Veterinary Institute of Serbia | Zujovic M.,Serbian Institute for Animal Husbandry | Tomic Z.,Serbian Institute for Animal Husbandry
CEFood 2012 - Proceedings of 6th Central European Congress on Food | Year: 2012

Goat's gastrointestinal helminths infection was of great importance to health status of goats and performances. This was parasitic infection caused by helminths from genus Ostertagia, Trichostrongylus, Nematodirus, Chabertia, Haemonchus, Marshallagia, Skrjabinema and Oesophagostomum. Goars infection had high prevalence, moderate morbidity and low mortality rate. Animals had abdominal pain, anemia, lost of appetite, dehydration, tenisms, weakens and lost of weight. Clinical signs are greenish or yellow diarrhea with smell, and some time is presented a blood. Young animals are depressive and inactive. Consequence is significant increase of kid accrescense, its weakens and less growth.

Pavlovic I.,Scientific Veterinary Institute of Serbia | Savic B.,Scientific Veterinary Institute of Serbia | Ivanovic S.,Scientific Veterinary Institute of Serbia | Cirovic D.,University of Belgrade
Journal of Wildlife Diseases | Year: 2012

Paramphistomum parasites identified by histology as Paramphistomum microbothrium were found in 18 of 34 roe deer (Capreolus capreolus) intestines from central Serbia, examined between 1998 and 2004. This represents the first record of P. microbothrium in roe deer in Serbia. © Wildlife Disease Association 2012.

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