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Carroll J.,Scientific Services Bureau
AFTE Journal | Year: 2013

Evidence from the medical examiner's office, consisting of ten dimes (United States 10 cent coins) and a modified shotshell wad, was submitted to the laboratory for examination and identification. This prompted a study into the history, feasibility, and practicality of the use of dimes as a projectile to be fired from shotguns.


Johnson D.J.,California State University, Los Angeles | Matthies L.K.,Scientific Services Bureau | Roberts K.A.,California State University, Los Angeles | Yorker B.C.,California State University, Los Angeles
Journal of Forensic Sciences | Year: 2010

Abortion specimens are often submitted to forensic laboratories as the only piece of physical evidence in rape and incest cases. The recovery of conceptus tissues from this evidence permits the use of paternity testing to evaluate suspects. In cases of abandoned newborns, the recovery of maternal tissue from the placenta allows for the direct comparison of genetic profiles between the suspected mother and the biological mother. We report on the identification and isolation of conceptus tissues from embryonic- and fetal-period abortions, and maternal tissues from delivered placentas, by gross and low-magnification examination with manual dissection. Hundreds of single-source samples have been successfully recovered by this method and short tandem repeat typed using standard forensic procedures. We additionally describe extraembryonic tissues that can be recovered and typed in the absence of the embryo proper. We conclude that an expertise and protocols can be developed by forensic laboratories for the routine analysis of this evidence. © 2010 American Academy of Forensic Sciences.


Johnson D.J.,California State University, Los Angeles | Andersen C.,Scientific Services Bureau | Scriven K.A.,800 Paseo Rancho Castilla | Klein A.N.,OC Crime Laboratory | And 3 more authors.
Journal of Forensic Sciences | Year: 2014

Bloodstain pattern analysis to determine the wound-of-origin of bloodstains is problematic with nonspecific patterns. In this proof-of-concept study, the authors examined a molecular approach to correlate bloodstains with injuries using the rat as a model. Specifically, investigations were conducted on the rat brain marker, rno-miR-124-3p, with the QIAGEN miScript System and real-time PCR analysis. Rno-miR-124-3p was detected in brain homogenates diluted 100,000 in 3-week-old, room temperature stored, simulated brain-blood stains; and in bloodstains from head gunshot wounds collected with swabs and subsequently frozen for 9-18 months; however, rno-miR-124-3p was not detected in whole blood. Proof-of-principle was demonstrated by the ability to distinguish bloodstains from a gunshot wound to the head versus bloodstains from a gunshot wound to the chest, by the testing of otherwise identical bloodstains from the two patterns for the presence of the marker. The results suggest a viable approach to a longstanding problem in casework. © 2014 American Academy of Forensic Sciences.

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