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Karbyshev G.L.,Scientific Research Antiplaque Institute | Simakova D.I.,Scientific Research Antiplaque Institute | Larionova L.V.,Scientific Research Antiplaque Institute | Terent'ev A.N.,Scientific Research Antiplaque Institute | And 2 more authors.
Applied Biochemistry and Microbiology | Year: 2011

A method for isolation of outer membrane proteins from Yersinia pseudotuberculosis, which are perspective for further application as sensitin for design of species-specific pseudotuberculosis antigenic diagnostic kits, has been modified. Common species-specific antigens of nine Y. pseudotuberculosis serovars (with molecular weight from 80.62 to 12.2 kDa) were detected by SDS-PAAGE electrophoresis and immunoblotting of the outer-membrane protein preparations. These antigens react with neither the rabbit experimental antiplague antiserum nor antiserum to 39 Y. enterocolitica serovars and normal rabbit serum. © 2011 Pleiades Publishing, Ltd.

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