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Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'Kov G.N.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Udut E.V.,Russian Academy of Medical Sciences | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2013

Pegylated hyaluronate-endo-β-N-acetylhexosaminidase was shown to potentiate significantly the hemostimulatory effect of pegylated erythropoietin. It was found that enhanced production of hemopoietin by adherent and non-adherent cells of the hemopoiesis-inducing microenvironment and elevated serum content of endogenous erythropoietin along with increased susceptibility of erythroid precursors to pegylated erythropoietin underlay this phenomenon. © 2013 Springer Science+Business Media New York.


Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'Kov G.N.,Russian Academy of Medical Sciences | Gurto R.V.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2013

We studied the effect of hyaluronate-endo-β-N-acetylhexosaminidase on the secretory function of the liver and bone marrow microenvironment cells in chronic hepatitis. Enhanced production of substances stimulating parenchymal tissue-specifi c precursors and stem cell homing factors by liver cells was revealed. At the same time, production of SDF-1 and other chemoattractants and adhesion factors of progenitor cells by bone marrow elements was reduced. © 2013 Springer Science+Business Media New York.


Dygai A.M.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Zyuzkov G.N.,Russian Academy of Medical Sciences | Stavrova L.A.,Russian Academy of Medical Sciences | And 15 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2012

High hepatoprotective activity of granulocytic CSF and hyaluronidase immobilized using electron-beam immobilization technology was demonstrated on the model of CCl4-induced hepatitis: the preparations produced anticholestatic, anti-inflammatory, and antisclerotic effects. These effects developed against the background of stimulation of bone marrow multipotent precursor cells and their mobilization into circulation accompanied by an increase in the content of parenchymatous progenitor cells in the liver. The most pronounced positive effect was observed in combined treatment with the test preparations. © 2012 Springer Science+Business Media, Inc.


Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'kov G.N.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Udut E.V.,Russian Academy of Medical Sciences | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2012

Pegylated hyaluronate-endo-β-N-acetylhexosaminidase considerably potentiates the hemostimulating effects of erythropoietin due to intensification of proliferation and differentiation of erythroid precursors against the background of enhanced secretion of hemopoietins by nonadherent hemopoiesis-inducing environment cells and elevation of serum erythropoietin concentration. The use of the enzyme allows 10-fold reduction of the maximum effective erythropoietin dose. © 2012 Springer Science+Business Media New York.


Dygai A.M.,Russian Academy of Medical Sciences | Skurikhin E.G.,Russian Academy of Medical Sciences | Ermakova N.N.,Russian Academy of Medical Sciences | Pershina O.V.,Russian Academy of Medical Sciences | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2013

Using the model of lung fibrosis induced by intratracheal administration of bleomycin we studied anti-fibrotic activity of combined treatment with neuroleptic haloperidol and hyaluronidase immobilized on polyethylene oxide using electron-beam synthesis. It was shown that successive administration of immobilized hyaluronidase and the neuroleptic drug inhibits deposition of collagen fibers in the bleomycin-treated lungs. Combined treatment with the test compounds reduced swelling of the alveolar epithelium, exudation and infiltration of the alveolar interstitium and alveolar passages by infl ammatory cells, and desquamation of alveolocytes into alveolar lumen, so that the alveolar-capillary membrane function was preserved. © 2013 Springer Science+Business Media New York.


Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'kov G.N.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Madonov P.G.,Scientific Future Management Company | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2011

Granulocytic CSF pegylated using electron-beam synthesis nanotechnology exhibits pronounced granulomonocytopoiesis-stimulating and SC-mobilizing activity. More potent stimulation of committed precursors against the background of less pronounced activation of polypotent hemopoietic cells is a peculiarity of hemostimulating action of pegylated using electron-beam synthesis nanotechnology granulocytic CSF in comparison with its non-modifi ed analog. The mobilizing effect of pegylated using electron-beam synthesis nanotechnology granulocytic CSF on early progenitor elements surpasses that of non-conjugated cytokine. © 2011 Springer Science+Business Media, Inc.


Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'kov G.N.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Udut E.V.,Russian Academy of Medical Sciences | And 12 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2011

In vitro experiments demonstrated increased colony-forming capacity of erythroid, granulo-monocytic, and mesenchymal progenitors of the bone marrow and parenchymal progenitor elements of the liver after treatment with immobilized hyaluronidase. Increased sensitivity of these progenitor cells to erythropoietin, granulocyte colony-stimulating factor, fibroblast growth factor, and stem cell factor, respectively, was demonstrated. Immobilized hyaluronidase enhanced the formation of tissue-specific hepatic CFU against the background of reduced yield of stromal precursors in liver tissue culture containing insulin. © 2011 Springer Science+Business Media, Inc.


Dygai A.M.,Russian Academy of Medical Sciences | Zyuz'kov G.N.,Russian Academy of Medical Sciences | Zhdanov V.V.,Russian Academy of Medical Sciences | Artamonov A.V.,Scientific Future Management Company | And 11 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2011

The hemostimulating effect of erythropoietin immobilized by the nanotechnology method of electron-beam synthesis was studied on the model of carboplatin-induced myelosuppression. Subcutaneous injection or oral administration of immobilized erythropoietin was followed by stimulation of erythropoiesis. The effect was most pronounced after parenteral treatment with this agent. The increase in proliferative activity and maturation of erythroid precursor cells serve as a factor determining acceleration of reparative processes in the hemopoietic tissue. © 2011 Springer Science+Business Media, Inc.

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