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Kawasaki, Japan

Inaoka Y.,Tokai University | Tajima A.,Tokushima University | Tamura T.,Scientific Crime Laboratory | Satoh F.,Tokai University | Osawa M.,Tokai University
Forensic Science International: Genetics Supplement Series | Year: 2011

Pairwise kinship analysis is the basic approach in forensic examinations. The conventional assessment is achieved after statistical calculation of genetic data from a set of STR markers. However, the combined index of likelihood ratio does not separate siblings from unrelated pairs. In the present study, we ensured the effectiveness of SNP microarray assay to the kinship analysis. Reproducible large-scale genotypes data were obtained by the assay. In the setting of more than 0.05 of minor allele frequency and r2=0.2 in linkage disequilibrium, the SNP data was analyzed using a IBD-based coefficient, π, in addition to IBD values. The minimum numbers of SNP subset for separation of full siblings and second degree pairs from unrelated pairs should be 1000 and 5000, respectively. SNP microarray assay will be useful for the analytic approach for full siblings and the second degree pairs. © 2011 Elsevier Ireland Ltd. Source


Azuma K.,Tottori University | Osaki T.,Tottori University | Ifuku S.,Tottori University | Saimoto H.,Tottori University | And 6 more authors.
Bioactive Carbohydrates and Dietary Fibre | Year: 2014

The aim of this study was to evaluate the effects of cellulose nanofibers from Japanese pear (P-CNF) on acute IBD by using a mouse model of this disorder. Furthermore, using this mouse model, we compared the effects between P-CNF and cellulose nanofibers from wood (W-CNF). P-CNF suppressed shortening of the colon length and improved the histological tissue injury in the mice. It also suppressed the activation of nuclear factor-kappa B and fibrosis of the colon, as well as the myeloperoxidase activation of inflammatory cells such as leukocytes. On the other hand, W-CNF did not improve the histological tissue injury, or suppress shortening of the colon length, colon inflammation, and fibrosis in the mice. These results revealed that P-CNF has anti-inflammatory effects in the experimental IBD mouse model. Our results indicate that P-CNF could be a potential source of new dietary fiber for patients with IBD. © 2013 Published by Elsevier Ltd. Source


Morimoto M.,Tottori University | Takatori M.,Tottori University | Hayashi T.,Tottori University | Mori D.,Tottori University | And 8 more authors.
Carbohydrate Research | Year: 2014

Fucoidan and chondroitin sulfate, which are well known sulfated polysaccharides, were depolymerized under hydrothermal conditions (120-180 C, 5-60 min) as a method for the preparation of sulfated polysaccharides with controlled molecular weights. Fucoidan was easily depolymerized, and the change of the molecular weight values depended on the reaction temperature and time. The degree of sulfation and IR spectra of the depolymerized fucoidan did not change compared with those of untreated fucoidan at reaction temperatures below 140 C. However, fucoidan was partially degraded during depolymerization above 160 C. Nearly the same depolymerization was observed for chondroitin sulfate. These results indicate that hydrothermal treatment is applicable for the depolymerization of sulfated polysaccharides, and that low molecular weight products without desulfation and deformation of the initial glycan structures can be obtained under mild hydrothermal conditions.©2013 Elsevier Ltd. All rights reserved. Source


Azuma K.,Tottori University | Ishihara T.,Tottori University | Nakamoto H.,Tottori University | Amaha T.,Tottori University | And 10 more authors.
Marine Drugs | Year: 2012

We evaluated the anti-tumor activities of the oral administration of fucoidan extracted from Cladosiphon okamuranus using a tumor (colon 26)-bearing mouse model. The materials used included low-molecular-weight fucoidan (LMWF: 6.5-40 kDa), intermediate-molecular-weight fucoidan (IMWF: 110-138 kDa) and high-molecular-weight fucoidan (HMWF: 300-330 kDa). The IMWF group showed significantly suppressed tumor growth. The LMWF and HMWF groups showed significantly increased survival times compared with that observed in the control group (mice fed a fucoidan-free diet). The median survival times in the control, LMWF, IMWF and HMWF groups were 23, 46, 40 and 43 days, respectively. It was also found that oral administration of fucoidan increased the population of natural killer cells in the spleen. Furthermore, from the results of the experiment using Myd-88 knockout mice, it was found that these effects are related to gut immunity. These results suggest that fucoidan is a candidate anti-tumor functional food. © 2012 by the authors; licensee MDPI, Basel, Switzerland. Source


Azuma K.,Tottori University | Osaki T.,Tottori University | Ifuku S.,Tottori University | Maeda H.,Tottori University | And 7 more authors.
Bioactive Carbohydrates and Dietary Fibre | Year: 2013

In this study, we investigated the suppressive effects of cellulose nanofibers made from adlay (A-CNF) and seaweed (S-CNF) on colon inflammation using the mouse model of inflammatory bowel disease (IBD). A-CNF and S-CNF suppressed shortening of the colon length and improved the histological tissue injury in mice. A-CNF and S-CNF also suppressed the activation of nuclear factor-kappa B in the colon. Furthermore, A-CNF and S-CNF suppressed the myeloperoxidase activities of inflammatory cells such as leukocytes. On the other hand, cellulose nanofibers made from wood did not improve the histological tissue injury or suppress the shortening of the colon length and colon inflammation in mice. These results revealed that A-CNF and S-CNF have suppressive effects on colon inflammation in an experimental IBD mouse model. Furthermore, our results indicate that A-CNF and S-CNF may be a potential source of dietary fiber for patients with IBD. © 2013 Elsevier Ltd. All rights reserved. Source

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