Scientific and Technical Research Center in Physico Chemical Analyses

Algiers, Algeria

Scientific and Technical Research Center in Physico Chemical Analyses

Algiers, Algeria

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Terbouche A.,Scientific and Technical Research Center in Physico Chemical Analyses | Terbouche A.,University of Science and Technology Houari Boumediene | Djebbar S.,University of Science and Technology Houari Boumediene | Benali-Baitich O.,University of Science and Technology Houari Boumediene | Bouet G.,Coordination Chemistry Laboratory
Soil and Sediment Contamination | Year: 2010

New humic acid isolated from forest soil of Yakouren (YHA), Algeria, and purified commercial humic acid (PCHA) were analyzed and characterized. Elemental analyses, electronic microscopy, UV-Vis absorbance E 465/E 665 ratio and molecular spectroscopy investigation using CP-MAS 13C-NMR of YHA indicated the presence of a high content of aliphatic and carboxylic acid groups. The higher O/C, H/C and N/C ratios of YHA can be assigned to the low aromatization degree. A study of interaction between Ni(II), Zn(II), Cd(II) and humic acids (HAs) at different metal-ligand ratios has been made by conductimetric and fluorescence techniques. The results obtained by the conductimetry method are interpreted using an excess function (δk), which related the conductivity of the mixture and of the separated components. A positive value of this function is obtained. It indicates the complexation of HAs with metallic ions. The association degree of the heavy metals (M) with both HAs was in the following order: Ni > Zn > Cd and the binding capacity of PCHA is smaller than that of YHA. A fluorescence titration method and a single site model were used for determining metal ion complexing capacities (CC M) and stability constants (log K M) of YHA and PCHA complexes. Titration of HAs with metal ions at pH 7 and ionic strength 0.1 mol/L resulted in a marked decrease of fluorescence intensities of untreated HAs. The quenching constants (K SV) between HAs and M are obtained in terms of Stern-Volmer analysis. K SV show that the YHA binds the higher amount metals than PCHA. CC M of YHA calculated by MATLAB program were higher than those of PCHA and other natural HAs found in the literature at pH 7. Based on these results, YHA may play the role of a biocaptor of polluting metals. © Taylor & Francis Group, LLC.


Terbouche A.,Scientific and Technical Research Center in Physico Chemical Analyses | Terbouche A.,University of Science and Technology Houari Boumediene | Terbouche A.,National Graduate School of Chemistry, Rennes | Djebbar S.,University of Science and Technology Houari Boumediene | And 3 more authors.
Water, Air, and Soil Pollution | Year: 2011

The complexation of heavy metals, present in their dissolved state at relevant trace levels, with new humic acids (HAs) isolated from Yakouren forest (YHA) and Sahara (Tamenrasset: THA) soils has been studied by differential pulse anodic stripping voltammetry (DPASV) at a hanging mercury drop electrode and conductimetry methods. After extraction and purification, humic acids were characterized by elemental analyses, atomic absorption spectroscopy, FT-IR, and solution state 13C-NMR. Taking Zn(II) and Cd(II) as examples, the aim of this study was to gain direct information on the general level of importance of humic acids for the speciation of certain heavy metals in soil to determine the complexing capacities of AHs and stability constant of the complexes formed with these metal ions and to compare the complexation capacity of forest and Sahara soils with the commercial humic acid and other published AHs. The results determined by conductimetry method are interpreted using an excess function (Δk) which related the conductivity of the mixture and of the separated components. A positive value of this function is obtained. It indicates the complexation of humic acids with metallic ions. The DPASV method was used for determining metal ion complexing capacities and stability constants of metal ion complexes of HAs in solution at pH 7. In both types of soils, the commercial humic acid (CHA) is less efficient in complexing Zn(II) and Cd(II) than THA and YHA and the complexing capacity (CC M) decreases in the order: THA > YHA > CHA. In general, the results of complexing capacity for all humic acids and stability constants of Zn(II) and Cd(II) complexes found by DPASV method showed good correlation with those of conductimetry method. CC M of THA and YHA calculated by DPASV were higher than those of CHA and the other natural HAs published in the literature at pH 7 basing on these results. © 2010 Springer Science+Business Media B.V.


Bouchoucha A.,University of Science and Technology Houari Boumediene | Terbouche A.,University of Science and Technology Houari Boumediene | Terbouche A.,Scientific and Technical Research Center in Physico Chemical Analyses | Zaouani M.,National Veterinary School of Algiers | And 2 more authors.
Journal of Trace Elements in Medicine and Biology | Year: 2013

The synthesis and characterization by elemental analysis, emission atomic spectroscopy, TG measurements, magnetic measurements, FTIR, 1H NMR, UV-visible spectra and conductivity of a series of iron (II) and nickel (II) complexes with two heterocyclic ligands (L1(SMX): sulfamethoxazole and L2(MIZ): metronidazole) used in pharmaceutical field and with a new ligand derived benzoxazole (L3(MPBO): 2-(5-methylpyridine-2-yl)benzoxazole), were reported. The formulae obtained for the complexes are: [M(L1)2 Cl2]·nH2O, [M(L2)2Cl2(H2O)2]·H2O and [M(L3)2(OH)2]·nH2O. Stability constants of these complexes have been determined by potentiometric methods in water-ethanol (90:10, v/v) mixture at a 0.2molL-1 ionic strength (NaCl) and at 25.0±0.1°C. Sirko program was used to determine the protonation constants as well as the binding constants of three species [ML2H2]2+, [ML2] and [ML]2+. The antimicrobial activity of the ligands and complexes was evaluated in vitro against different human bacteria and fungi using agar diffusion method.Iron sulfamethoxazole complex showed a remarkable inhibition of bacteria growth especially on Staphylococcus aureus and P. aeruginosa. The iron metronidazole complex is active against yeasts especially on Candida tropicalis strain. Nickel complexes presented different antibacterial and antifungal behavior's against bacteria and fungal.The acute toxicity study revealed that the iron complexes are not toxic at 2000. mg/kg dose orally administrated.LD50 for nickel complexes was determined using graphical method.No significant differences in the body weights between the control and the treated groups of both rat sexes in subacute toxicity study using for iron complexes. Hematological and clinical blood chemistry analysis revealed no toxicity effects of the iron complexes. Pathologically, neither gross abnormalities nor histopathological changes were observed for these complexes. © 2013 Elsevier GmbH.


Djouahri A.,University of Science and Technology Houari Boumediene | Boualem S.,University of Science and Technology Houari Boumediene | Boudarene L.,University of Science and Technology Houari Boumediene | Boudarene L.,Scientific and Technical Research Center in Physico Chemical Analyses | And 2 more authors.
Industrial Crops and Products | Year: 2015

The present study reports the variation of the essential oils composition from wood and leaves of Tetraclinis articulata (Vahl) Masters, collected from four locations in Algeria as well as compares their antioxidant and anti-inflammatory activities. 34 compounds were identified, accounting for 90.6-97.6% of the leaves oils. The predominant compounds were α-pinene (16.3-25.3%), camphor (17.2-21.4%) and bornyl acetate (17.6-20.6%). 29 compounds were identified, accounting for 91.2-95.5% of the total wood oil. Camphene (15.5-18.8%), cedrol (10.5-12.7%) and α-acorenol (8.2-10.4%) were the main compounds. The Principal Component Analysis (PCA) revealed chemical variability in the studied essential oils. Although the main components are common, their percentages are different. The investigated essential oils showed substantial antioxidant and anti-inflammatory activities. Although the two assays yielded quantitatively different values, the best antioxidant and anti-inflammatory activities was observed for oil from Zeddine region flowed by that from Mansoura region, while, the lowest activities was observed for oil from Tazoult region. In this work, we revealed significant differences in the yields and chemical composition. Furthermore, our study demonstrates a high variation in the biological activity potentials according to parts of species and populations. This variability points out the importance of the geographic origin of the populations and the growing conditions which could affect significantly the oil compounds both in quantitative or qualitative extent and by there the biological activities. It offers also the opportunity to choose essential oils with preferential compound for pharmaceutical, perfume and food industries. © 2014 Elsevier B.V.


Djouahri A.,University of Science and Technology Houari Boumediene | Boudarene L.,University of Science and Technology Houari Boumediene | Boudarene L.,Scientific and Technical Research Center in Physico Chemical Analyses | Meklati B.Y.,Scientific and Technical Research Center in Physico Chemical Analyses
Industrial Crops and Products | Year: 2013

Two extraction methods, microwave-assisted hydrodistillation (MAHD) and conventional hydrodistillation (HD), were used to extract the volatile compounds from the leaves of Tetraclinis articulata (Vahl) Masters, which were subsequently analyzed by GC and GC-MS. The results of both oils were compared in terms of extraction time, extraction yield/efficiency, chemical composition, antioxidant and anti-inflammatory activities. MAHD is more advantageous than HD in terms of energy savings and extraction time (90. min vs. 180. min for MAHD vs. HD), extraction yield (0.54% vs. 0.56% for MAHD vs. HD). There were significant differences between the quantities of the main abundant compounds. The oil of MAHD was characterized by a higher amounts of oxygenated compounds, while, the oil of HD was characterized by a higher amounts of hydrocarbons compounds. It was found that the essential oil of MAHD exhibited higher antioxidant and anti-inflammatory activities than the essential oil of HD. These results suggest that these oils might be a valuable source of bioactive compounds, and would seem to be applicable in both the health and food, feasible alternatives as antioxidant and anti-inflammatory agents. Significantly lower energy consumption with MAHD renders this technology being more environmentally friendly than HD. Compared to many solvent extraction techniques such as Soxhlet and accelerated solvent extraction, MAHD is modern, green and fast. © 2012.


Djouahri A.,University of Science and Technology Houari Boumediene | Saka B.,University of Science and Technology Houari Boumediene | Boudarene L.,University of Science and Technology Houari Boumediene | Boudarene L.,Scientific and Technical Research Center in Physico Chemical Analyses | And 8 more authors.
Industrial Crops and Products | Year: 2014

The aim of this work was to study for the first time the phenolic composition and synergistic/antagonistic antibacterial and anti-inflammatory effect between various extracts/essential oil from cones of Tetraclinis articulata (Vahl) Masters and antibiotic/anti-inflammatory agents. 12 components were identified in essential oil, which are mostly oxygenated monoterpenes (94.47%). HPLC-DAD analysis showed that the tannic acid (0.45. ±. 0.038. 1.85. ±. 0.18. μg/g) and gallic acid (0.72. ±. 0.044. - 1.98. ±. 0.20. μg/g) are present in all extracts. The ethanol 70% extract was the most effective against the tested bacteria and exhibits the highest anti-inflammatory activity. Even, the combination of antibiotic with this extract was more effective, showing synergism or partial synergism effect in 100% against the tested bacteria. While, the methanol 100% extract was less effective, showing antagonism effect in 100% against the tested bacteria. In the same way, the combination of the specify inhibitors of lipooxygenase and xanthine oxidase (NDGA/allopurinol) with ethanol 70% extract was more effective, showing synergism inhibition effect against both enzymes. According to these results, the ethanol 70% extract may represent a potential valuable source of bioactive and anti-inflammatory agents and an alternative for the treatment of infectious diseases caused by the tested bacteria and the inflammation where the lipooxygenase and xanthine oxidase are involved. Even, this extract can be used in combination with the antibiotics and anti-inflammatory agents to give synergism effect, which allow us to reduce their effective antimicrobial and anti-inflammatory dose and thereby reducing their undesirable problems and side effects. © 2014 Elsevier B.V.

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